Rna Structure And Folding

Author : Dagmar Klostermeier,Christian Hammann
Publisher : Walter de Gruyter
Page : 432 pages
File Size : 51,6 Mb
Release : 2013-10-14
Category : Science
ISBN : 9783110284959

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RNA Structure and Folding by Dagmar Klostermeier,Christian Hammann Pdf

While structure-function relationships of proteins have been studied for a long time, structural studies of RNA face additional challenges. Nevertheless, with the continuous discovery of novel RNA molecules with key cellular functions and of novel pathways and interaction networks, the need for structural information of RNA is still increasing. This volume provides an introduction into techniques to assess structure and folding of RNA. Each chapter explains the theoretical background of one technique, and illustrates possibilities and limitations in selected application examples.

Author : Daniel Herschlag
Publisher : Academic Press
Page : 496 pages
File Size : 54,6 Mb
Release : 2009-11-16
Category : Science
ISBN : 0080922139

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Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: by Daniel Herschlag Pdf

This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. Expert researchers introduce the most advanced technologies and techniques in protein structure and folding Includes techniques on tiling assays

Author : Neocles Leontis,Eric Westhof
Publisher : Springer Science & Business Media
Page : 402 pages
File Size : 46,9 Mb
Release : 2012-06-05
Category : Science
ISBN : 9783642257407

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RNA 3D Structure Analysis and Prediction by Neocles Leontis,Eric Westhof Pdf

With the dramatic increase in RNA 3D structure determination in recent years, we now know that RNA molecules are highly structured. Moreover, knowledge of RNA 3D structures has proven crucial for understanding in atomic detail how they carry out their biological functions. Because of the huge number of potentially important RNA molecules in biology, many more than can be studied experimentally, we need theoretical approaches for predicting 3D structures on the basis of sequences alone. This volume provides a comprehensive overview of current progress in the field by leading practitioners employing a variety of methods to model RNA 3D structures by homology, by fragment assembly, and by de novo energy and knowledge-based approaches.

Author : Rick Russell
Publisher : Springer Science & Business Media
Page : 238 pages
File Size : 55,9 Mb
Release : 2012-12-09
Category : Science
ISBN : 9781461449546

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Biophysics of RNA Folding by Rick Russell Pdf

This volume, written by experts in the field, discusses the current understanding of the biophysical principles that govern RNA folding, with featured RNAs including the ribosomal RNAs, viral RNAs, and self-splicing introns. In addition to the fundamental features of RNA folding, the central experimental and computational approaches in the field are presented with an emphasis on their individual strengths and limitations, and how they can be combined to be more powerful than any method alone; these approaches include NMR, single molecule fluorescence, site-directed spin labeling, structure mapping, comparative sequence analysis, graph theory, course-grained 3D modeling, and more. This volume will be of interest to professional researchers and advanced students entering the field of RNA folding.

Author : Daniel Herschlag
Publisher : Academic Press
Page : 567 pages
File Size : 50,5 Mb
Release : 2009-11-16
Category : Science
ISBN : 9780123809230

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Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B by Daniel Herschlag Pdf

This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. Expert researchers introduce the most advanced technologies and techniques in protein structure and folding Includes techniques on tiling assays

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 45,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Christina Waldsich
Publisher : Humana
Page : 0 pages
File Size : 52,5 Mb
Release : 2016-08-23
Category : Medical
ISBN : 149396058X

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RNA Folding by Christina Waldsich Pdf

Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.

Author : Ronny Lorenz
Publisher : Springer Nature
Page : 407 pages
File Size : 44,6 Mb
Release : 2024-06-29
Category : Electronic
ISBN : 9781071635193

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RNA Folding by Ronny Lorenz Pdf

Author : Douglas H. Turner,David H. Mathews
Publisher : Humana
Page : 0 pages
File Size : 40,9 Mb
Release : 2016-09-24
Category : Medical
ISBN : 1493964313

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RNA Structure Determination by Douglas H. Turner,David H. Mathews Pdf

This volume provides protocols and procedures for determining and modeling RNA structure. Chapters guide the reader through protocols for RNA secondary structure prediction, single sequence modeling, Crumple, RNAstructure to model conserved secondary structures with multiple homologs, the prediction of bimolecular secondary structures with RNAstructure, STarMir, protocols for structure mapping, mapping data to constrain or restrain RNA secondary structure prediction with RNAstructure, unassigned NMR resonances, modeling protocols for Rosetta FARFAR, RNAComposer , ModeRNA, and MC-Fold. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, RNA Structure Determination: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : Christina Waldsich
Publisher : Unknown
Page : 340 pages
File Size : 45,5 Mb
Release : 2014
Category : RNA
ISBN : 1627036679

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RNA Folding by Christina Waldsich Pdf

Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.

Author : Risa Karakida Kawaguchi,Junichi Iwakiri
Publisher : Springer Nature
Page : 304 pages
File Size : 45,7 Mb
Release : 2023-01-27
Category : Science
ISBN : 9781071627686

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RNA Structure Prediction by Risa Karakida Kawaguchi,Junichi Iwakiri Pdf

This book explores recent progress in RNA secondary, tertiary structure prediction, and its application from an expansive point of view. Because of advancements in experimental protocols and devices, the integration of new types of data as well as new analysis techniques is necessary, and this volume discusses additional topics that are closely related to RNA structure prediction, such as the detection of structure-disrupting mutations, high-throughput structure analysis, and 3D structure design. Written for the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed implementation advice that leads to quality research results. Authoritative and practical, RNA Structure Prediction serves as a valuable guide for both experimental and computational RNA researchers.

Author : Jienyu Ding,Jason R. Stagno,Yun-Xing Wang
Publisher : Springer Nature
Page : 256 pages
File Size : 47,6 Mb
Release : 2022-10-13
Category : Science
ISBN : 9781071626870

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RNA Structure and Dynamics by Jienyu Ding,Jason R. Stagno,Yun-Xing Wang Pdf

This volume provides a wide spectrum of multidisciplinary approaches for studying RNA structure and dynamics, including detailed accounts of experimental and computational procedures. Chapters guide readers through cryo-electron microscopy, crystallography, isothermal titration calorimetry, small angle X-ray scattering, single-molecule Förster Energy transfer, X-ray free electron laser, atomic force microscopy, computational simulation, and prediction. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, RNA Structure and Dynamics aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Author : Nils Walter,Sarah A. Woodson,Robert T. Batey
Publisher : Springer Science & Business Media
Page : 400 pages
File Size : 44,5 Mb
Release : 2008-11-18
Category : Science
ISBN : 9783540708407

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Non-Protein Coding RNAs by Nils Walter,Sarah A. Woodson,Robert T. Batey Pdf

The 2006 Nobel Prize in Physiology or Medicine was awarded to the discoverers of RNA interference, Andrew Fire and Craig Mello. This prize, which follows “RNA” Nobels for splicing and RNA catalysis, highlights just one class of recently discovered non-protein coding RNAs. Remarkably, non-coding RNAs are thought to outnumber protein coding genes in mammals by perhaps as much as four-fold. In fact, it appears that the complexity of an organism correlates with the fraction of its genome devoted to non-protein coding RNAs. Essential biological processes as diverse as cell differentiation, suppression of infecting viruses and parasitic tra- posons, higher-level organization of eukaryotic chromosomes, and gene expression are found to be largely directed by non-protein coding RNAs. Currently, bioinformatic, high-throughput sequencing, and biochemical approaches are identifying an increasing number of these RNAs. Unfortunately, our ability to characterize the molecular details of these RNAs is significantly lacking. The biophysical study of these RNAs is an emergent field that is unraveling the molecular underpinnings of how RNA fulfills its multitude of roles in sustaining cellular life. The resulting understanding of the physical and chemical processes at the molecular level is critical to our ability to harness RNA for use in biotechnology and human therapy, a prospect that has recently spawned a multi-billion dollar industry.

Author : Angus I. Lamond
Publisher : Springer Science & Business Media
Page : 230 pages
File Size : 51,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783662223253

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Pre-mRNA Processing by Angus I. Lamond Pdf

he past fifteen years have seen tremendous growth in our understanding of T the many post-transcriptional processing steps involved in producing func tional eukaryotic mRNA from primary gene transcripts (pre-mRNA). New processing reactions, such as splicing and RNA editing, have been discovered and detailed biochemical and genetic studies continue to yield important new insights into the reaction mechanisms and molecular interactions involved. It is now apparent that regulation of RNA processing plays a significant role in the control of gene expression and development. An increased understanding of RNA processing mechanisms has also proved to be of considerable clinical importance in the pathology of inherited disease and viral infection. This volume seeks to review the rapid progress being made in the study of how mRNA precursors are processed into mRNA and to convey the broad scope of the RNA field and its relevance to other areas of cell biology and medicine. Since one of the major themes of RNA processing is the recognition of specific RNA sequences and structures by protein factors, we begin with reviews of RNA-protein interactions. In chapter 1 David Lilley presents an overview of RNA structure and illustrates how the structural features of RNA molecules are exploited for specific recognition by protein, while in chapter 2 Maurice Swanson discusses the structure and function of the large family of hnRNP proteins that bind to pre-mRNA. The next four chapters focus on pre-mRNA splicing.

Author : Daniel Herschlag
Publisher : Unknown
Page : 400 pages
File Size : 41,6 Mb
Release : 2009
Category : Proteins
ISBN : 0123743990

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Biophysical Approaches to RNA Structure and Folding by Daniel Herschlag Pdf

This volume provides laboratory techniques that aim to predict the structure of a protein which can have implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells.