Short Protocols In Molecular Biology

Author : Frederick M. Ausubel
Publisher : Current Protocols
Page : 1140 pages
File Size : 46,9 Mb
Release : 1999-05-03
Category : Medical
ISBN : UOM:39015048647104

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Short Protocols in Molecular Biology by Frederick M. Ausubel Pdf

Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include: * Informatics for Molecular Biologists * Analysis of Protein Interactions * Epitope Tagging * Mathematics and Statistics for Molecular Biologists Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.

Author : Frederick M. Ausubel
Publisher : Unknown
Page : 1020 pages
File Size : 43,8 Mb
Release : 1987
Category : Molecular biology
ISBN : UOM:39015041905467

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Current Protocols in Molecular Biology by Frederick M. Ausubel Pdf

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 47,6 Mb
Release : 2024-06-27
Category : Molecular biology
ISBN : 047150338X

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Current Protocols in Molecular Biology by Anonim Pdf

Author : Frederick M. Ausubel
Publisher : Unknown
Page : 128 pages
File Size : 45,9 Mb
Release : 2003-05-01
Category : Electronic
ISBN : 0555012417

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Current Protocols in Molecular Biology Core by Frederick M. Ausubel Pdf

Author : Frederick M. Ausubel
Publisher : Unknown
Page : 854 pages
File Size : 44,5 Mb
Release : 1992
Category : Biología molecular
ISBN : UCSD:31822007888142

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Short Protocols in Molecular Biology by Frederick M. Ausubel Pdf

Author : Frederick M. Ausubel
Publisher : Unknown
Page : 128 pages
File Size : 48,9 Mb
Release : 2003-05-01
Category : Electronic
ISBN : 0555012433

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Current Protocols in Molecular Biology Tabs by Frederick M. Ausubel Pdf

Author : Frederick M. Ausubel
Publisher : Wiley-Interscience
Page : 422 pages
File Size : 42,7 Mb
Release : 1989-09-25
Category : Science
ISBN : UOM:39015042058654

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Short Protocols in Molecular Biology by Frederick M. Ausubel Pdf

This volume contains shortened versions of the methods published in the looseleaf manual Current Protocols in Molecular Biology. It presents fully-tested, current techniques based on material from the core manual and from the quarterly update service. Includes all step-by-step descriptions of methods covered in the first ten chapters of CPMB and provides enough detail to perform the experiments (only introductions, annotations, and commentary have been omitted). Marginal notes explain the hows and whys of many steps, and provide tips on safety, storage, and anticipated results. Includes references and recipes for all reagents and media and helpful tables and illustrations.

Author : Frederick M. Ausubel
Publisher : Unknown
Page : 128 pages
File Size : 46,5 Mb
Release : 2002-04-01
Category : Electronic
ISBN : 0555001407

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Current Protocols in Molecular Biology Tabs Reprint by Frederick M. Ausubel Pdf

Author : Frederick M. Ausubel
Publisher : Current Protocols
Page : 924 pages
File Size : 44,9 Mb
Release : 1995-10-06
Category : Medical
ISBN : UOM:39015037279778

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Short Protocols in Molecular Biology by Frederick M. Ausubel Pdf

A desktop companion to the three-volume Current Protocols in Molecular Biology, the recognized leader in bioscience laboratory manuals. This edition contains over 220 protocols from leading laboratories worldwide. All methods are lab-tested and include step-by-step instructions, equipment and materials necessary to successfully conduct an experiment.

Author : Sean R. Gallagher,Emily A. Wiley
Publisher : John Wiley & Sons
Page : 679 pages
File Size : 55,8 Mb
Release : 2012-03-19
Category : Science
ISBN : 9780470942413

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Current Protocols Essential Laboratory Techniques by Sean R. Gallagher,Emily A. Wiley Pdf

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Author : John M. S. Bartlett,David Stirling
Publisher : Springer Science & Business Media
Page : 1083 pages
File Size : 49,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593842

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PCR Protocols by John M. S. Bartlett,David Stirling Pdf

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Author : Bruce A. White
Publisher : Springer Science & Business Media
Page : 397 pages
File Size : 54,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595020

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PCR Protocols by Bruce A. White Pdf

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

Author : Sandeep Singh,Dhiraj Kumar
Publisher : Bentham Science Publishers
Page : 186 pages
File Size : 54,7 Mb
Release : 2020-01-23
Category : Science
ISBN : 9789811439292

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Protocols used in Molecular Biology by Sandeep Singh,Dhiraj Kumar Pdf

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Author : John E. Coligan
Publisher : Current Protocols
Page : 846 pages
File Size : 53,8 Mb
Release : 1991
Category : Immunology
ISBN : UCLA:L0064730518

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Current Protocols in Immunology by John E. Coligan Pdf

Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, assays for cytokines and their receptors, isolation and analysis of proteins and peptides, biochemistry of cell activation, molecular immunology, and animal models of autoimmune and inflammatory diseases. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Bimonthly updates, which are filed into the looseleaf, keep the set current with the latest developments in immunology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.

Author : Juan S. Bonifacino
Publisher : Wiley-Blackwell
Page : 844 pages
File Size : 52,7 Mb
Release : 2004-02-10
Category : Science
ISBN : UOM:39015058708820

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Short Protocols in Cell Biology by Juan S. Bonifacino Pdf

Providing condensed descriptions of more than 500 methods compiled from Current Protocols in Cell Biology, this text thoroughly explores cell biology in an easily accessible, hands-on format. Short Protocols in Cell Biology is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of cell biology methods. Key Features: Designed to provide quick access to step-by-step instructions for the essential methods used in every major area of cell biological research Contains methods from every aspect of cell biology?everything needed to study the basic structure and functions of cells at both the molecular and cellular levels