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Somatic Embryogenesis: Fundamental Aspects and Applications by Víctor M Loyola-Vargas,Neftalí Ochoa-Alejo Pdf
This book discusses basic and applied aspects of somatic embryogenesis, one of the most powerful tools in plant biotechnology. It is divided into three parts; Part I includes topics such as the history of this research field, how differentiated plant cells can (re)acquire totipotency, molecular features, as well as the epigenetics and proteomics of somatic embryogenesis. Part II covers the somatic embryogenesis of different crops, such as Agave spp. maize, Cocos nucifera, Bixa orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and Arabidopsis thaliana. Various applications, like scale-up propagation and genetic engineering are discussed in detail in Part III. The book will appeal to plant scientists, plant breeders and experts working in industry.
Somatic Embryogenesis by Abdul Mujib,Jozef Šamaj Pdf
Somatic embryogenesis, the initiation of embryos from previously differentiated somatic cells, is a unique process in plants. This volume expands our view of a subject that is important for plant biotechnology, genetics, cell biology, development, and agricultural applications. All chapters present the latest research progress, including functional genomic, genetic, and proteomic approaches. A special focus is placed on the effects of stress, environment, and plant growth regulators on embryogenesis. The role of genes such as Leafy Cotyledons and Baby Boom in defining and maintaining cell competence is discussed.
Somatic Embryogenesis by Marco A. Ramírez-Mosqueda Pdf
This volume provides an overview of methods for plant species, mainly species with high economic value, to achieve in vitro propagation through somatic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species to this morphogenetic route. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Embryogenesis: Methods and Protocols aims to guide future researcher to achieve success in obtaining and regenerating somatic embryos. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Somatic Embryogenesis in Woody Plants by S. Mohan Jain,Pramod K. Gupta,R.J. Newton Pdf
The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com mercial companies. Generally, tissue culture of woody plants has been recal citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalurn album), Citrus, Mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propa gation for large-scale production of plants or "emblings" or "somatic embryo plants", b) protoplast work, c) cryopreservation, d) genetic transformation, and e) artificial or manufactured seed production.
Author : Professor Dr. Y. P. S. Bajaj Publisher : Springer Science & Business Media Page : 504 pages File Size : 44,8 Mb Release : 1995-05-16 Category : Technology & Engineering ISBN : 3540574484
Somatic Embryogenesis and Synthetic Seed I by Professor Dr. Y. P. S. Bajaj Pdf
While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro propagation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.
Protocol for Somatic Embryogenesis in Woody Plants by Shri Mohan Jain,Pramod K. Gupta Pdf
World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.
Somatic Embryogenesis and Gene Expression by Junaid Aslam,Prem S. Srivastava,M. P. Sharma Pdf
Provides an insight into current advances in the development of somatic embryogenesis, as well as the cellular and molecular mechanism, differential gene expression, and extracellular protein markers during different developing stages of somatic embryogenesis. It cover somatic embryogenesis in dicotyledons and monocotyledons plants, different factors regulating somatic embryogenesis (plant genotype, level of sugar in the medium, type and concentration of growth regulators, photoperiod, gelling agents, time exposure, induction and maturation medium), application of complex organic additives (often involves fruit juices, pulp, extracts and homogenates as well as liquid endosperms, protein hydrolysates like casein hydrolysate, tryptone, and yeast lysate like yeast extract), genetic fidelity of somatic embryos derived plantlets and role of connecting stress leading in expression of transcription factor that appears to connect stress and developmental signalling pathways and is essential for the subsequent signal transduction cascade that leads to the formation of somatic embryos. Expression of different genes associated with the accumulation of storage reserve during somatic embryogenesis along with isolation and molecular characterization of miRNAs at different developmental stages of embryogenesis, and finally proteomics during somatic embryogenesis has been discussed in detail.
Author : Professor Dr. Y. P. S. Bajaj Publisher : Springer Science & Business Media Page : 489 pages File Size : 49,8 Mb Release : 2013-03-14 Category : Technology & Engineering ISBN : 9783662030912
Somatic Embryogenesis and Synthetic Seed I by Professor Dr. Y. P. S. Bajaj Pdf
While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro propagation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.
Somatic Embryogenesis in Woody Plants by S.M. Jain,Pramod P.K. Gupta,R.J. Newton Pdf
The rapid progress made on somatic embryogenesis and its prospects for potential applications in improving woody plants prompted us to edit this book initially in three volumes, and now to add two more volumes. The editors were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This Volume 4 has been divided into three sections and contains 23 chapters. Section A contains eleven chapters covering studies of embryo development and cell biology of white spruce, proliferative somatic embryogenesis in woody species, somatic embryo germination and desiccation tolerance in conifers, performance of conifer somatic seedlings, apoptosis during early somatic embryogenesis, water relation parameters in conifer embryos, image analysis of somatic embryos, somatic embryogenesis in woody legumes, cold storage and cryopreservation, and commercialization of plant somatic embryogenesis. Section B contains six chapters dealing with angiosperm woody plants, such as somatic embryogenesis in myrtaceous plants, Laurus nobilis, Simarouba glauca, Magnolia spp., Juglans cinera, and somatic embryogenesis and evaluation of variability in somatic seedlings of Quercus serrata by RAPD markers. The chapters contained in Section C are focused on somatic embryogenesis in gymnosperms, including Pinus patula, Encephalartos, Picea wilsonii, Pinus banksiana, hybrid firs, and Taxus. All the chapters have been peer-reviewed and revised accordingly to improve their quality.
Protocol for Somatic Embryogenesis in Woody Plants by Shri Mohan Jain,Pramod K. Gupta Pdf
World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.
Somatic Embryogenesis in Woody Plants by S. Mohan Jain,Pramod P.K. Gupta,R.J. Newton Pdf
These books provide an update to progress on somatic embryogenesis in woody plants including both angiosperm and gymnosperm trees. In the past, most of the information on this subject was scattered in proceedings volumes, journals, biotechnology books, etc. It has been difficult for the researchers and students to obtain comprehensive information on this rapidly growing subject from a single source. These books enable readers to get a clear view of this subject on historical, anatomical, physiological, biochemical and molecular aspects, and applications including protoplasts, cryopreservation, manufactured seed (artificial seed), genetic transformation, bioreactors, mutations, and future uses in forest plantations. Each selected woody plant mentioned in the book is briefly introduced first, covering botany and genetics, importance and geographical distribution, breeding problems, and in vitro propagation and problems of each selected woody plant and then is followed by the description on the initiation and maintenance of embryogenic cultures, embryo development and germination, and field trials (if any) of these plants. These books are meant for graduate students and researchers in forestry and horticulture as well as biotechnologists.
Somatic Embryogenesis in Woody Plants by S. Mohan Jain,P.K. Gupta,R.J. Newton Pdf
The rapid progress on somatic embryogenesis and its prospects for potential application to improving woody plants prompted us to edit this book initially in three volumes, and now an additional three more volumes. We were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This volume 6 is dedicated to Prof. Harry Waris, Helsinki, Finland, who did pioneer work on somatic embryogenesis during the time when Prof. Steward and others were actively engaged in this area. His former student Prof. Liisa Simols, University of Helsinki, Finland, has written a dedication `Harry Waris, a pioneer in somatic embryogenesis' to her teacher Prof. Waris. This volume is divided into three sections and contains a total of 26 chapters. Section A comprises seven chapters covering topics such as: Historical insights into some contemporary problems in somatic embryogenesis (SE); Thin cell layer for somatic embryogenesis induction in woody trees; SE in tropical fruit and forest trees; SE in fruit and forest arid trees; Status of SE in Indian forest trees; SE research in fruit trees in India; Applications of SE for the improvement of tropical fruit trees. Section B comprises 15 chapters, dealing with: SE in oil palm, hazelnut (Corylus avellana L.), pistachio (Pistacia vera L.), Araucaria angustifolia, Quercus suber, Aspidosperma polyneuron, Acacia senegal, Simmondsia chiensis, Cupressus sempervirens, pecan (Carya illinoinensis), rattan (Calamus spp.), tamarillo (Cyphomandra betacea, longan (Dimocarpus longan Lor.), Aegle marmelos, and Euonymus europaeus. Section C comprises three chapters related to cryo-storage of citrus, conifers and rubber. All the chapters have been peer-reviewed and revised accordingly to improve the quality of the chapters. We are thankful to all: (a) contributory authors for their co-operation in submitting manuscripts in time, and (b) reviewers for spending their valuable time in reviewing the manuscripts.
Somatic Embryogenesis in Woody Plants by S.M. Jain,Pramod P.K. Gupta,R.J. Newton Pdf
The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environmental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding could become a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Generally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus and mango (Mangifera indica), offer a ray of hope for inexpensive clonal propagation for large-scale production of plants or 'emblings' or somatic seedlings; protoplast work; cryopreservation; genetic transformation; and synthetic or artificial or manufactured seed production.
Somatic Embryogenesis and Synthetic Seed II by Y. P. S. Bajaj Pdf
While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro prop a gation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.
Somatic Embryogenesis in Woody Plants by S.M. Jain,P.K. Gupta,R.J. Newton Pdf
The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.
