Tandem Mass Spectrometry

Author : Michael Kinter,Nicholas E. Sherman
Publisher : John Wiley & Sons
Page : 321 pages
File Size : 48,8 Mb
Release : 2005-04-12
Category : Science
ISBN : 9780471231882

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Protein Sequencing and Identification Using Tandem Mass Spectrometry by Michael Kinter,Nicholas E. Sherman Pdf

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more

Author : Robert C Murphy
Publisher : Royal Society of Chemistry
Page : 280 pages
File Size : 54,7 Mb
Release : 2014-12-02
Category : Science
ISBN : 9781782626350

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Tandem Mass Spectrometry of Lipids by Robert C Murphy Pdf

The emerging field of lipidomics has been made possible because of advances in mass spectrometry, and in particular tandem mass spectrometry of lipid ions generated by electrospray ionization. The ability to carry out basic biochemical studies of lipids using electrospray ionization is predicated upon understanding the behaviour of lipid derived ions following collision induced decomposition and mechanisms of product ion formation. During the past 20 years, a wealth of information has been generated about lipid molecules that are now analysed by mass spectrometry, however there is no central source where one can obtain basic information about how these very diverse biomolecules behave following collisional activation. This book brings together, in one volume, this information so that investigators considering using tandem mass spectrometry to structurally characterize lipids or to quantitate their occurrence in a biological matrix, will have a convenient source to review mechanism of decomposition reactions related to the diversity of lipid structures. A separate chapter is devoted to each of seven major lipid classes including fatty acids, eicosanoids and bioactive lipid mediators, fatty acyl esters and amides, glycerol esters, glycerophospholipids, sphingolipids, and steroids. Mechanistic details are provided for understanding the pathways of formation of major product ions and ions used for structural characterization. In most cases specific ancillary information has been critical to understand the pathways, including isotope labeling and high resolution analysis of precursor and product ions. For a few specific examples such data is missing and pathways are proposed as a means to initiate further mass spectral experiments to prove or disprove pathway hypotheses. While this work largely centres on the lipid biochemistry of animal (mammalian) systems, general principles can be taken from the specific examples and applied to lipid biochemistry found in plants, fungi, prokaryotes and archeal organisms.

Author : Kenneth L. Busch,Gary L. Glish,Scott A. McLuckey
Publisher : Wiley-VCH
Page : 360 pages
File Size : 43,6 Mb
Release : 1988
Category : Science
ISBN : UOM:39015016085675

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Mass Spectrometry/mass Spectrometry by Kenneth L. Busch,Gary L. Glish,Scott A. McLuckey Pdf

Author : Jürgen H Gross
Publisher : Springer Science & Business Media
Page : 534 pages
File Size : 43,5 Mb
Release : 2006-04-05
Category : Science
ISBN : 9783540407393

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Mass Spectrometry by Jürgen H Gross Pdf

Mass Spectrometry is an ideal textbook for students and professionals as well as newcomers to the field. Starting from the very first principles of gas-phase ion chemistry and isotopic properties, the textbook takes the reader through the design of mass analyzers and ionization methods all the way to mass spectral interpretation and coupling techniques. Step-by-step, the reader learns how mass spectrometry works and what it can do. The book comprises a balanced mixture of practice-oriented information and theoretical background. It features a clear layout and a wealth of high-quality figures. Exercises and solutions are located on the Springer Global Web.

Author : Ana Varela Coelho,Catarina De Matos Ferraz Franco
Publisher : BoD – Books on Demand
Page : 208 pages
File Size : 42,6 Mb
Release : 2013-05-29
Category : Medical
ISBN : 9789535111368

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Tandem Mass Spectrometry by Ana Varela Coelho,Catarina De Matos Ferraz Franco Pdf

Tandem Mass Spectrometry - Molecular Characterization presents a comprehensive coverage of theory, instrumentation and description of experimental strategies and MS/MS data interpretation for the structural characterization of relevant molecular compounds. The areas covered include the analysis of drugs, metabolites, carbohydrates and protein post-translational modifications. The book series in Tandem Mass Spectrometry serves multiple groups of audiences; professional (academic and industry), graduate students and general readers interested in the use of modern mass spectrometry in solving critical questions of chemical and biological sciences.

Author : Haroun N. Shah,Saheer E. Gharbia
Publisher : John Wiley & Sons
Page : 648 pages
File Size : 42,9 Mb
Release : 2017-03-31
Category : Science
ISBN : 9781118960233

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MALDI-TOF and Tandem MS for Clinical Microbiology by Haroun N. Shah,Saheer E. Gharbia Pdf

This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

Author : Oscar Alzate
Publisher : CRC Press
Page : 356 pages
File Size : 52,5 Mb
Release : 2009-10-26
Category : Medical
ISBN : 9781420076264

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Neuroproteomics by Oscar Alzate Pdf

In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg

Author : Fred W. McLafferty
Publisher : Wiley-Interscience
Page : 536 pages
File Size : 54,9 Mb
Release : 1983
Category : Science
ISBN : UOM:39015050440497

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Tandem Mass Spectrometry by Fred W. McLafferty Pdf

This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1909 edition. Excerpt: ... well satisfied with the impression he had made upon all concerned. Bertha had mounted to her room and waited, first sending her maid away. Presently her door had been opened and her husband entered, and for an hour she had been listening to his outburst of distorted jealousy. The attack was finally losing its force, and turning a shoulder she seated herself at the dressingtable and began to cut the threads with which a jeweled ornament was sewn to her bodice. In the mirror his face looked at her with all the sneering, abandoned malevolence to which she was so accustomed. "If you are quite through, I will ring for my maid," she said at last. "Im through for now, but you and your discarded lover--your former kept man" She cut a thread very carefully. "Ill have the truth from one of you or the other--do you hear me, you common hussy? You vile street" His elbow brushed some roses. He dashed the vase to the floor and stamped petals and Sevres into the rug. She unhooked her pearl collar, and drew it from her neck. "All I want you to do," said the hoarse whine behind her--the mans anger was evidently ex "In the mirror his face looked at her with all the sneering. malevolence to which she was so accustomed." hausted on the vase--"all I ask of you is just to confess. Tell me that he was your lover and I will ask you no more--just say it--tell me that he was--tell me that, and Ill be satisfied." She laid the collar in its case. Would a lie" soothe him? She knew it would not. He would want details. She smiled with the thought of the whole mad, foolish, trivial thing. With a scream he clutched her. She twisted away, but his fist in her laces tore them to the girdle. Still holding the torn strip, he thrust his twitching face close to hers. Her...

Author : Marek Smoluch,Giuseppe Grasso,Piotr Suder,Jerzy Silberring
Publisher : John Wiley & Sons
Page : 450 pages
File Size : 47,9 Mb
Release : 2019-07-11
Category : Science
ISBN : 9781119377306

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Mass Spectrometry by Marek Smoluch,Giuseppe Grasso,Piotr Suder,Jerzy Silberring Pdf

Provides a comprehensive description of mass spectrometry basics, applications, and perspectives Mass spectrometry is a modern analytical technique, allowing for fast and ultrasensitive detection and identification of chemical species. It can serve for analysis of narcotics, counterfeit medicines, components of explosives, but also in clinical chemistry, forensic research and anti-doping analysis, for identification of clinically relevant molecules as biomarkers of various diseases. This book describes everything readers need to know about mass spectrometry—from the instrumentation to the theory and applications. It looks at all aspects of mass spectrometry, including inorganic, organic, forensic, and biological MS (paying special attention to various methodologies and data interpretation). It also contains a list of key terms for easier and faster understanding of the material by newcomers to the subject and test questions to assist lecturers. Knowing how crucial it is for young researchers to fully understand both the power of mass spectrometry and the importance of other complementary methodologies, Mass Spectrometry: An Applied Approach teaches that it should be used in conjunction with other techniques such as NMR, pharmacological tests, structural identification, molecular biology, in order to reveal the true function(s) of the identified molecule. Provides a description of mass spectrometry basics, applications and perspectives of the technique Oriented to a broad audience with limited or basic knowledge in mass spectrometry instrumentation, theory, and its applications in order to enhance their competence in this field Covers all aspects of mass spectrometry, including inorganic, organic, forensic, and biological MS with special attention to application of various methodologies and data interpretation Includes a list of key terms, and test questions, for easier and faster understanding of the material Mass Spectrometry: An Applied Approach is highly recommended for advanced students, young scientists, and anyone involved in a field that utilizes the technique.

Author : Edmond de Hoffmann,Vincent Stroobant
Publisher : John Wiley & Sons
Page : 502 pages
File Size : 48,9 Mb
Release : 2013-04-03
Category : Science
ISBN : 9781118681947

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Mass Spectrometry by Edmond de Hoffmann,Vincent Stroobant Pdf

The latest edition of a highly successful textbook, Mass Spectrometry, Third Edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analysers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter. Emphasis is placed throughout the text on optimal utilisation conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra. This new edition has been thoroughly revised and updated and has been redesigned to give the book a more contemporary look. As with previous editions it contains numerous examples, references and a series of exercises of increasing difficulty to encourage student understanding. Updates include: Increased coverage of MALDI and ESI, more detailed description of time of flight spectrometers, new material on isotope ratio mass spectrometry, and an expanded range of applications. Mass Spectrometry, Third Edition is an invaluable resource for all undergraduate and postgraduate students using this technique in departments of chemistry, biochemistry, medicine, pharmacology, agriculture, material science and food science. It is also of interest for researchers looking for an overview of the latest techniques and developments.

Author : Chhabil Dass
Publisher : John Wiley & Sons
Page : 512 pages
File Size : 48,8 Mb
Release : 2007-05-11
Category : Science
ISBN : 9780470118481

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Fundamentals of Contemporary Mass Spectrometry by Chhabil Dass Pdf

Modern mass spectrometry - the instrumentation and applications in diverse fields Mass spectrometry has played a pivotal role in a variety of scientific disciplines. Today it is an integral part of proteomics and drug discovery process. Fundamentals of Contemporary Mass Spectrometry gives readers a concise and authoritative overview of modern mass spectrometry instrumentation, techniques, and applications, including the latest developments. After an introduction to the history of mass spectrometry and the basic underlying concepts, it covers: Instrumentation, including modes of ionization, condensed phase ionization techniques, mass analysis and ion detection, tandem mass spectrometry, and hyphenated separation techniques Organic and inorganic mass spectrometry Biological mass spectrometry, including the analysis of proteins and peptides, oligosaccharides, lipids, oligonucleotides, and other biological materials Applications to quantitative analysis Based on proven teaching principles, each chapter is complete with a concise overview, highlighted key points, practice exercises, and references to additional resources. Hints and solutions to the exercises are provided in an appendix. To facilitate learning and improve problem-solving skills, several worked-out examples are included. This is a great textbook for graduate students in chemistry, and a robust, practical resource for researchers and scientists, professors, laboratory managers, technicians, and others. It gives scientists in diverse disciplines a practical foundation in modern mass spectrometry.

Author : Edmond de Hoffmann,Vincent Stroobant
Publisher : Wiley
Page : 420 pages
File Size : 50,5 Mb
Release : 2001-10-10
Category : Science
ISBN : 0471485659

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Mass Spectrometry by Edmond de Hoffmann,Vincent Stroobant Pdf

Offers a complete overview of the principles, theories and key applications of modern mass spectrometry in this introductory textbook. Following on from the highly successful first edition, this edition is extensively updated including new techniques and applications. All instrumental aspects of mass spectrometry are clearly and concisely described; sources, analysers and detectors. * Revised and updated * Numerous examples and illustrations are combined with a series of exercises to help encourage student understanding * Includes biological applications, which have been significantly expanded and updated * Also includes coverage of ESI and MALDI

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 54,8 Mb
Release : 1993
Category : Electronic
ISBN : OCLC:80111535

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Tandem Mass Spectrometry by Anonim Pdf

Author : Jeroen Kool,Wilfried M. A. Niessen
Publisher : John Wiley & Sons
Page : 402 pages
File Size : 48,8 Mb
Release : 2015-05-04
Category : Science
ISBN : 9783527334643

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Analyzing Biomolecular Interactions by Mass Spectrometry by Jeroen Kool,Wilfried M. A. Niessen Pdf

This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.

Author : Kenneth L. Busch,Gary L. Glish,Scott A. McLuckey
Publisher : Unknown
Page : 360 pages
File Size : 46,9 Mb
Release : 1988
Category : Mass spectrometry
ISBN : UCSD:31822004036471

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Mass Spectrometry/mass Spectrometry by Kenneth L. Busch,Gary L. Glish,Scott A. McLuckey Pdf