Techniques In Free Radical Research

Author : A.T. Diplock,M.C.R. Symons,C.A. Rice-Evans
Publisher : Elsevier
Page : 290 pages
File Size : 53,9 Mb
Release : 1991-12-11
Category : Science
ISBN : 0080858910

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Techniques in Free Radical Research by A.T. Diplock,M.C.R. Symons,C.A. Rice-Evans Pdf

Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage. Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.

Author : Catherine A. Rice-Evans
Publisher : Unknown
Page : 291 pages
File Size : 41,6 Mb
Release : 1991
Category : Electronic
ISBN : 0720442001

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Techniques in Free Radical Research by Catherine A. Rice-Evans Pdf

Author : Robert A. Greenwald
Publisher : CRC Press
Page : 467 pages
File Size : 55,5 Mb
Release : 2018-02-01
Category : Medical
ISBN : 9781351081375

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Handbook Methods For Oxygen Radical Research by Robert A. Greenwald Pdf

This volume is divided into five sections. Section I deals with preparative methodology for isolation and purification of the components of the oxy radical experimental systems use most frequently, including all three forms of SOD and several other important scavengers. Section II provides the experimenter with a choice of a dozen oxy radical generating systems which can be used for testing of scavengers or for evaluation of the effects of oxy radicals on target tissues. The reader will note that in this section, as well as in most of the rest of the volume, the orientation is primarily biochemical, biologic, and medical, rather than pure chemistry or pure physics. Nevertheless, the techniques are widely applicable to a variety of disciplines.

Author : Robert A. Greenwald
Publisher : Unknown
Page : 447 pages
File Size : 49,8 Mb
Release : 1987
Category : Electronic
ISBN : OCLC:246072183

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CRC Handbook of Methods for Oxygen Radical Research by Robert A. Greenwald Pdf

Author : John Schostak,Jill Schostak
Publisher : Routledge
Page : 499 pages
File Size : 40,5 Mb
Release : 2007-09-05
Category : Education
ISBN : 9781134149483

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Radical Research by John Schostak,Jill Schostak Pdf

Radical Research explores the view that research is not a neutral tool to be employed without bias in the search for truth. Rather the radical roots of research are to be seen in the focus on freedom and emancipation from blind allegiance to tradition, ‘common sense’, religion, or powerful individuals and organisations. Radical Research introduces and draws upon leading contemporary debates and data gathered from a diversity of funded projects in; health, education, police training, youth and community, schools, business, and the use of information technology. This book presents a radical view of research in a way that enables both beginner and the experienced professional researcher to explore its approaches in the formation of their own views and practices. It progressively leads the reader from discussions of case studies to critical explorations of the philosophical and methodological concepts, theories and arguments that are central to contemporary debates. In essence, this book shows how to design, develop and write radical research under conditions where ‘normal’ research rules apply and it offers a ground-breaking and proven alternative to traditional research techniques.

Author : Lester Packer
Publisher : Elsevier
Page : 758 pages
File Size : 53,9 Mb
Release : 1994
Category : Active oxygen
ISBN : 012182134X

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Oxygen Radicals in Biological Systems by Lester Packer Pdf

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features* Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical* Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damage.

Author : Neville A. Punchard,Frank J. Kelly
Publisher : IRL Press
Page : 310 pages
File Size : 43,6 Mb
Release : 1996
Category : History
ISBN : 0199635595

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Free Radicals by Neville A. Punchard,Frank J. Kelly Pdf

With the recognition that oxygen and nitrogen radicals are involved in normal cell metabolism, free radical research has begun to feature in most disciplines in the life sciences. Increasingly, their implication in a number of human disease processes is being accepted, altough conclusive evidence is lacking in many instances, partly due to the difficulties in detecting and measuring free radicals that have lifetimes measured in microseconds. This book outlines the latest techniques for quantifying radicals and their effects, and features detailed protocols, hints, and tips for success, troubleshooting comments, sample data, and key literature citations.

Author : William Pryor
Publisher : Elsevier
Page : 304 pages
File Size : 50,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323156820

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Free Radicals in Biology V1 by William Pryor Pdf

Free Radicals in Biology, Volume 1, provides an introduction to some of the basic concepts of free radical biology. The book emerged from the President's Symposium of the American Society for Experimental Pathology held in Atlantic City in April 1970. The book's opening chapter introduces a mechanistic view of free radical reactions using examples from biological systems. This is followed by a discussion of autoxidative damage to lipids in membranes. Some of the special chemistry of lipid oxidation are presented, and a number of novel ideas are suggested. The third chapter deals with the uses of electron-spin resonance in biology. Spin labeling is also discussed, which is one of the most useful methods of probing the chemical and physical environment of molecules in cells. The remaining chapters cover free radicals which occur in photosynthesis and the importance of the enzyme, superoxide dismutase. The study of the occurrence and reactions of this enzyme provides an important and extensive body of data clearly pointing to the significance of free radicals in cellular chemistry.

Author : Ana Čipak Gašparović
Publisher : MDPI
Page : 192 pages
File Size : 49,5 Mb
Release : 2020-06-18
Category : Science
ISBN : 9783039360888

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Free Radical Research in Cancer by Ana Čipak Gašparović Pdf

Cancer is a great challenge to efficient therapy due to biological diversity. Disturbed oxidative homeostasis in cancer cells certainly contributes to differential therapy response. Further, one of the hallmarks of cancer cells is adaptation which includes fine tuning of the cellular metabolic and signalling pathways as well as transcription profiles. There are several factors which contribute to the tumor diversity and therapy response, and oxidative stress is certainly one of them. Changes in oxygen levels due to hypoxia/reoxygenation during tumor growth modulate antioxidative patterns finally supporting increased cell diversity and adaptation to stressing conditions. Additionally, cancer chemotherapy based on ROS production can also induce also adaptation. To counteract these negative effects natural products are often used for their antioxidant activities as well as photodynamic therapy supported by novel chemosensitizers. Understanding of possible pathways which can trigger antioxidant defence at a certain time during cancer development can also provide possible strategies in fighting cancer.

Author : Free Radical Research Society. Meeting
Publisher : Unknown
Page : 266 pages
File Size : 53,6 Mb
Release : 1985
Category : Electronic
ISBN : OCLC:756229387

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Free Radicals in Biology and Medicine by Free Radical Research Society. Meeting Pdf

Author : Alain E. Favier,Jean Neve,Patrice Faure
Publisher : The American Oil Chemists Society
Page : 330 pages
File Size : 50,9 Mb
Release : 1994-05-30
Category : Medical
ISBN : 0935315535

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Trace Elements and Free Radicals in Oxidative Diseases by Alain E. Favier,Jean Neve,Patrice Faure Pdf

Author : A. Favier,J. Cadet,B. Kalyanaraman,M. Fontecave,J.L. Pierre
Publisher : Birkhäuser
Page : 314 pages
File Size : 54,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783034890748

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Analysis of Free Radicals in Biological Systems by A. Favier,J. Cadet,B. Kalyanaraman,M. Fontecave,J.L. Pierre Pdf

"Oxidative stress" is used as the generic term describing the involve ment of reactive oxygen species in various human diseases. The scope of such a topic is becoming increasingly wide. The recent interest in radicals such as nitric oxide and the discovery of new mechanisms such as the effect of free radicals on redox sensitive proteins and genes are enlarging our understanding of the physiological role of free radicals. Oxidative stress is involved in numerous pathological. processes such as ageing, respiratory or cardiovascular diseases, cancer, neurological pathologies such as dementia or Parkinson's disease. It still remains difficult, however, to demonstrate by chemical measurement the in vivo production of free radicals and even more to realise their speciation. Therefore, the development of new tools and indicators is engrossing many researchers working in this field. Reliable indicators are abso lutely necessary not only to monitor the evolution of oxidative stress in patients but also to evaluate the efficiency of new antioxidant treat ments. The French Free radical club of Grenoble, the CERLIB has been involved for many years in the organisation of international training programs on methodology, in order to provide both theoretical and practical help to researchers from various countries. Such training sessions have been highly successful and participants value the oppor tunity to learn reliable techniques. This positive echo explains why the researchers of CERLIB decided, with the help of Prof. Dr. B. Kalyanaraman, to publish selected techniques on free radical re search.

Author : Malcolm D. Forbes
Publisher : John Wiley & Sons
Page : 300 pages
File Size : 40,6 Mb
Release : 2010-01-22
Category : Science
ISBN : 0470584106

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Carbon-Centered Free Radicals and Radical Cations by Malcolm D. Forbes Pdf

Covers the most advanced computational and experimental methods for studying carbon-centered radical intermediates With its focus on the chemistry of carbon-centered radicals and radical cations, this book helps readers fully exploit the synthetic utility of these intermediates in order to prepare fine chemicals and pharmaceutical products. Moreover, it helps readers better understand their role in complex atmospheric reactions and biological systems. Thoroughly up to date, the book highlights the most advanced computational and experimental methods available for studying and using these critically important intermediates. Carbon-Centered Free Radicals and Radical Cations begins with a short history of the field of free radical chemistry, and then covers: A discussion of the relevant theory Mechanistic chemistry, with an emphasis on synthetic utility Molecular structure and mechanism, focusing on computational methods Spectroscopic investigations of radical structure and kinetics, including demonstrations of spin chemistry techniques such as CIDNP and magnetic field effects Free radical chemistry in macromolecules Each chapter, written by one or more leading experts, explains difficult concepts clearly and concisely, with references to facilitate further investigation of individual topics. The authors were selected in order to provide insight into a broad range of topics, including small molecule synthesis, polymer degradation, computational chemistry as well as highly detailed experimental work in the solid, liquid, and gaseous states. This volume is essential for students or researchers interested in building their understanding of the role of carbon-centered radical intermediates in complex systems and how they may be used to develop a broad range of useful products.

Author : International Society for Free Radical Research. Congress,Society for Free Radical Research International,International Society for Free Radical Research
Publisher : Unknown
Page : 352 pages
File Size : 43,7 Mb
Release : 2006
Category : Medical
ISBN : 8875872740

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Thirteenth Congress of the Society for Free Radical Research International by International Society for Free Radical Research. Congress,Society for Free Radical Research International,International Society for Free Radical Research Pdf

Author : Y. Robert Li
Publisher : Bentham Science Publishers
Page : 343 pages
File Size : 47,5 Mb
Release : 2012-03-31
Category : Medical
ISBN : 9781608053223

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Free Radical Biomedicine: Principles, Clinical Correlations, and Methodologies by Y. Robert Li Pdf

Free radicals and related reactive species, including reactive oxygen and nitrogen species (ROS/RNS) play a critical role in the pathophysiological processes of various human diseases, including cardiovascular diseases, diabetes and metabolic syndrome, neurological disorders, and cancer among many others. This peer-reviewed E-book covers both the fundamental principles and the recent advances in free radical biomedicine along with in-depth discussions of the clinical correlations. It also provides a thorough coverage of the commonly used methods in free radical and antioxidant research with detailed schematic illustrations as well as succinct descriptions of the procedures. This volume should also be a valuable source of information for readers who wish to gain a sound understanding of the research methodologies in this rapidly evolving field.