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Author : Stephen A. Bustin
Publisher : Cambridge University Press
Page : 327 pages
File Size : 40,8 Mb
Release : 2010
Category : Science
ISBN : 9780521882316
Examines the latest innovations and the overall impact of PCR on areas of molecular research.
Author : Sarika Garg
Publisher : Arcler Press
Page : 0 pages
File Size : 46,9 Mb
Release : 2017-11
Category : Electronic
ISBN : 1773611232
The polymerase chain reaction (PCR) is a widely used molecular biology technique. It was invented in 1983 by Kary Mullis, who was awarded a Nobel Prize in Chemistry in 1993 for his innovation. Since its introduction, the PCR technology has brought a true revolution in scientific research involving applied areas such as diagnosis and genetic improvements for plants and animals. PCR is a method of replicating deoxyribonucleic acid (DNA) by the selective amplification of a target region of a DNA molecule. Only few cycles of PCR can generate millions of copies of a particular DNA sequence quickly and precisely. Routinely, a PCR reaction consists of three distinct steps- denaturation, annealing and extension. In the first step of denaturation (at 95°C), double stranded DNA denatures into two single strands. In the annealing step (at 56°C), short complementary sequences of DNA known as primers, anneal to the single stranded target DNA. In the final step of extension, temperature is raised to around 72℃. DNA polymerase extends the primer to form a second complimentary strand of DNA. The elaboration of a PCR technique for amplification and for analyzing even very small amounts of DNA or a damaged DNA has generated wide applications in analysis of gene, diagnosis of several genetic diseases and detection of infectious agents like viruses, bacteria, fungi, protozoa, etc. Another beneficial application of PCR is in the generation of a genetic fingerprint. PCR is also used to monitor pathogens in the environment. PCR is an imperative technique for research in molecular biology such as in cloning procedure which has considerable potential in forensic science. PCR has numerous applications in various other fields such as agriculture sciences, personalized medicine, archeology, etc. This book gathers the recently published works related to PCR and its applications. The first chapter highlights the introduction to polymerase chain reaction. The second chapter features the application of reverse transcriptase-polymerase chain reaction (RT-PCR) in the rapid detection of pathogen in water samples. In chapter 3, discussion is on the PCR methods useful for accurate and rapid screening of the genetically modified organisms in foods. PCR applications in forensic science are described in chapter 4. Application of PCR for the diagnosis of ankylosing spondylitis is discussed in chapter 5. In chapters 6-12, use of PCR technique for the detection of pathogens and diagnosis of infectious diseases is described. PCR methods for the detection of Alzheimer's disease-related single nucleotide polymorphism and cancer related genes are illustrated in chapters 13 and 14, respectively. Chapter 15 discusses the PCR in exploring endodontic infections. Analysis of the archaeological skeletal remains using PCR is described in the last chapter of the book. I hope that this book will be advantageous for students, researchers, teachers, and industrial experts.
Author : National Research Council,Division on Engineering and Physical Sciences,Commission on Physical Sciences, Mathematics, and Applications,Committee on the Mathematical Sciences in Genome and Protein Structure Research
Publisher : National Academies Press
Page : 300 pages
File Size : 45,6 Mb
Release : 1995-04-06
Category : Science
ISBN : 9780309048866
As researchers have pursued biology's secrets to the molecular level, mathematical and computer sciences have played an increasingly important roleâ€"in genome mapping, population genetics, and even the controversial search for "Eve," hypothetical mother of the human race. In this first-ever survey of the partnership between the two fields, leading experts look at how mathematical research and methods have made possible important discoveries in biology. The volume explores how differential geometry, topology, and differential mechanics have allowed researchers to "wind" and "unwind" DNA's double helix to understand the phenomenon of supercoiling. It explains how mathematical tools are revealing the workings of enzymes and proteins. And it describes how mathematicians are detecting echoes from the origin of life by applying stochastic and statistical theory to the study of DNA sequences. This informative and motivational book will be of interest to researchers, research administrators, and educators and students in mathematics, computer sciences, and biology.
Author : Robert Lindstrom,Jill Scarson
Publisher : Unknown
Page : 128 pages
File Size : 40,9 Mb
Release : 2017-07
Category : Electronic
ISBN : 153233172X
Author : Tania Nolan,Stephen A. Bustin
Publisher : CRC Press
Page : 461 pages
File Size : 52,8 Mb
Release : 2013-06-13
Category : Law
ISBN : 9781439848050
PCR’s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology. Topics in this forward-thinking volume include: The purification and handling of PCR templates The effect of the manufacture and purification of the oligonucleotide on PCR behavior Optimum buffer composition Probe options The design and optimization of qPCR assays Issues surrounding the development and refinement of instrumentation Effective controls to protect against uncertainties due to reaction variability Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.
Author : Paul Rabinow
Publisher : University of Chicago Press
Page : 200 pages
File Size : 54,8 Mb
Release : 2011-11-27
Category : Science
ISBN : 9780226216874
Making PCR is the fascinating, behind-the-scenes account of the invention of one of the most significant biotech discoveries in our time—the polymerase chain reaction. Transforming the practice and potential of molecular biology, PCR extends scientists' ability to identify and manipulate genetic materials and accurately reproduces millions of copies of a given segment in a short period of time. It makes abundant what was once scarce—the genetic material required for experimentation. Making PCR explores the culture of biotechnology as it emerged at Certus Corporation during the 1980s and focuses on its distinctive configuration of scientific, technical, social, economic, political, and legal elements, each of which had its own separate trajectory over the preceding decade. The book contains interviews with the remarkable cast of characters who made PCR, including Kary Mullin, the maverick who received the Nobel prize for "discovering" it, as well as the team of young scientists and the company's business leaders. This book shows how a contingently assembled practice emerged, composed of distinctive subjects, the site where they worked, and the object they invented. "Paul Rabinow paints a . . . picture of the process of discovery in Making PCR: A Story of Biotechnology [and] teases out every possible detail. . . . Makes for an intriguing read that raises many questions about our understanding of the twisting process of discovery itself."—David Bradley, New Scientist "Rabinow's book belongs to a burgeoning genre: ethnographic studies of what scientists actually do in the lab. . . . A bold move."—Daniel Zalewski, Lingua Franca "[Making PCR is] exotic territory, biomedical research, explored. . . . Rabinow describes a dance: the immigration and repatriation of scientists to and from the academic and business worlds."—Nancy Maull, New York Times Book Review
Author : Henry Erlich
Publisher : Springer
Page : 246 pages
File Size : 41,5 Mb
Release : 2015-12-31
Category : Science
ISBN : 9781349202355
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Author : Ferric C. Fang,Arturo Casadevall
Publisher : John Wiley & Sons
Page : 549 pages
File Size : 53,5 Mb
Release : 2023-10-09
Category : Science
ISBN : 9781683674368
Thinking about Science: Good Science, Bad Science, and How to Make It Better A riveting exploration of the world of science, diving headfirst into its triumphs and tribulations. Penned by seasoned microbiologists Ferric C. Fang and Arturo Casadevall, this book offers a comprehensive analysis of the scientific enterprise through various lenses, including historical, philosophical, and personal. From their unique vantage points as researchers, clinicians, and educators, Fang and Casadevall dissect the intricate mechanisms of science, shedding light on its strengths and weaknesses. Through engaging historical anecdotes, personal narratives, and insightful academic studies, they present a candid evaluation of sciences performance, including a thought-provoking examination of its role during the COVID-19 pandemic. A must-read for anyone curious about the present predicaments and future potential of science, Thinking about Science: Good Science, Bad Science, and How to Make It Better is more than just a book; its a roadmap to understanding and improving the scientific endeavor for the benefit of society at large. The authors have given us a thoughtful description of science and the joy of discovery, an unflinching diagnosis of where improvements are needed, and recommendations for remedies well worth considering. Scientists, science and society would benefit if this book were read by both future and established scientists, as well as the administrators, policymakers, and regulators who are in a position to help us do better. Michael Kalichman, UC San Diego With a deep understanding of the profound impact of science on society, the authors provide thought-provoking perspectives on changes in the scientific enterprise that will support sustainable, equitable practices, and engender public trust. An engaging read for everyone with an interest in science or science policy. Stanley Maloy, San Diego State University
Author : John McGreal
Publisher : Troubador Publishing Ltd
Page : 284 pages
File Size : 54,5 Mb
Release : 2017-04-18
Category : Art
ISBN : 9781788033541
John McGreal's three new books – It’s Reproduction, Contently, It’s Revolution, Actively and It’s Transformation, Contently – continue the ‘It’ Series published by Matador since 2010.
Author : Kary B. Mullis,Francois Ferre,Richard A. Gibbs
Publisher : Springer Science & Business Media
Page : 464 pages
File Size : 55,9 Mb
Release : 2012-02-02
Category : Medical
ISBN : 9781461202578
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Author : Douglas D. Colwell,Martin J. R. Hall,Philip J. Scholl
Publisher : CABI
Page : 371 pages
File Size : 47,6 Mb
Release : 2006
Category : Science
ISBN : 9781845931681
This book provides an in-depth review and analysis of the biology of adults and larvae of the Family Oestridae (commonly known as botflies, or warble flies). Oestrid flies cause myiasis (invasion of living tissue by the larvae), and are a major pest of both domestic and wild animals worldwide. The book presents a comparative investigation of the life histories and adaptation to parasitism exhibited by this unique family of flies. It also gives a detailed survey of each genus and provides a synopsis of the taxonomy of the family. It contains chapters on morphology, life history, host-parasite relationships, taxonomy and behaviour.
Author : Lorette C. Javois
Publisher : Springer Science & Business Media
Page : 928 pages
File Size : 53,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592135
Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
Author : Tania Nolan,Stephen A. Bustin
Publisher : CRC Press
Page : 457 pages
File Size : 48,9 Mb
Release : 2013-06-13
Category : Law
ISBN : 9781439848135
PCR's simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that
Author : C. Simon Herrington,John J. O'Leary
Publisher : Oxford University Press
Page : 223 pages
File Size : 50,8 Mb
Release : 1997-10-30
Category : Science
ISBN : 9780191565595
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
Author : Stephen J Meltzer
Publisher : Springer Science & Business Media
Page : 286 pages
File Size : 55,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592595754
PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.