Tissue Culture Technique

Author : Bernice M. Martin
Publisher : Springer Science & Business Media
Page : 251 pages
File Size : 51,7 Mb
Release : 2013-12-01
Category : Medical
ISBN : 9781461202479

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Tissue Culture Techniques by Bernice M. Martin Pdf

ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Author : Robert H. Smith
Publisher : Academic Press
Page : 190 pages
File Size : 43,8 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323160476

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Plant Tissue Culture by Robert H. Smith Pdf

Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Author : Jennie P. Mather,Penelope E. Roberts
Publisher : Springer Science & Business Media
Page : 247 pages
File Size : 45,7 Mb
Release : 2007-08-20
Category : Science
ISBN : 9780585275710

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Introduction to Cell and Tissue Culture by Jennie P. Mather,Penelope E. Roberts Pdf

It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Author : Kenneth C. Torres
Publisher : Springer Science & Business Media
Page : 282 pages
File Size : 50,7 Mb
Release : 2012-12-06
Category : Technology & Engineering
ISBN : 9781461597568

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Tissue Culture Techniques for Horticultural Crops by Kenneth C. Torres Pdf

This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.

Author : Paul F. Jr. Kruse
Publisher : Elsevier
Page : 897 pages
File Size : 40,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323142076

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Tissue Culture by Paul F. Jr. Kruse Pdf

Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Author : Maureen A. Harrison,Ian F. Rae
Publisher : Cambridge University Press
Page : 178 pages
File Size : 54,6 Mb
Release : 1997-10-13
Category : Science
ISBN : 052157496X

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General Techniques of Cell Culture by Maureen A. Harrison,Ian F. Rae Pdf

Concise introduction to a major technique of cell biology laboratories for those new to the field.

Author : Saurabh Bhatia,Kiran Sharma,Randhir Dahiya,Tanmoy Bera
Publisher : Academic Press
Page : 452 pages
File Size : 47,6 Mb
Release : 2015-07-22
Category : Science
ISBN : 9780128024980

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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences by Saurabh Bhatia,Kiran Sharma,Randhir Dahiya,Tanmoy Bera Pdf

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions

Author : Roberta H. Smith
Publisher : Gulf Professional Publishing
Page : 260 pages
File Size : 44,8 Mb
Release : 2000
Category : Nature
ISBN : 0126503427

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Plant Tissue Culture by Roberta H. Smith Pdf

This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media.

Author : Gladys Cameron
Publisher : Academic Press
Page : 205 pages
File Size : 55,9 Mb
Release : 2013-09-03
Category : Medical
ISBN : 9781483269030

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Tissue Culture Technique by Gladys Cameron Pdf

Tissue Culture Technique, Second Edition, provides an introduction to tissue culture techniques. An attempt has been made to reduce all equipment and procedure to their simplest forms without omitting steps necessary to ensure successful cultures. Sufficient detail is given to enable acquisition of the essentials of the techniques and avoidance of the many pitfalls which may be encountered by beginners, and may sometimes beset those more experienced. The first few chapters of this book are devoted to the choice and organization of the laboratory rooms and their equipment, including glassware, instruments, etc. Attention is also given to methods of preparation of supplies for use in the various techniques. The succeeding chapters describe the preparation of the culture media and the tissues, as well as the preparation of the cultures in various ways. Also discussed are the types of cells one may expect to see growing from a given tissue, methods of recording their behavior and measuring their growth, as well as their significance in the interpretation of experimental results. The last chapters treat methods of applying micrurgical, histological, and photomicrographic techniques to tissue cultures. It is hoped that the carefully considered data presented in this book, and the many details which are the result of long experience, may be of real service to the prospective worker.

Author : Roberta H. Smith
Publisher : Academic Press
Page : 206 pages
File Size : 42,5 Mb
Release : 2013
Category : Science
ISBN : 9780124159204

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Plant Tissue Culture by Roberta H. Smith Pdf

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Author : B. N. Sathyanarayana,Dalia B. Varghese
Publisher : I. K. International Pvt Ltd
Page : 299 pages
File Size : 51,7 Mb
Release : 2007
Category : Botany, Experimental
ISBN : 9788189866112

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Plant Tissue Culture by B. N. Sathyanarayana,Dalia B. Varghese Pdf

Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Author : Michael R. Davey,Paul Anthony
Publisher : John Wiley & Sons
Page : 358 pages
File Size : 45,9 Mb
Release : 2010-05-20
Category : Science
ISBN : 0470686510

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Plant Cell Culture by Michael R. Davey,Paul Anthony Pdf

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Author : Bernice Michaelene Martin
Publisher : Birkhauser
Page : 247 pages
File Size : 46,8 Mb
Release : 1994-01-01
Category : Tissue culture
ISBN : 3764336439

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Tissue Culture Techniques by Bernice Michaelene Martin Pdf

Author : M.P. Singh,Sunil Kumar
Publisher : APH Publishing
Page : 294 pages
File Size : 43,7 Mb
Release : 2009
Category : Plant tissue culture
ISBN : 8131304396

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Plant Tissue Culture by M.P. Singh,Sunil Kumar Pdf

Author : Víctor M. Loyola-Vargas,Felipe Vázquez-Flota
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 53,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592599592

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Plant Cell Culture Protocols by Víctor M. Loyola-Vargas,Felipe Vázquez-Flota Pdf

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.