Topics In Fluorescence Spectroscopy

Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 346 pages
File Size : 50,6 Mb
Release : 2005-06-28
Category : Science
ISBN : 0387233342

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Advanced Concepts in Fluorescence Sensing by Chris D. Geddes,Joseph R. Lakowicz Pdf

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 50,9 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461576587

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 524 pages
File Size : 51,5 Mb
Release : 1994-11-30
Category : Science
ISBN : 9780306447846

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Topics in Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 460 pages
File Size : 52,8 Mb
Release : 2007-12-29
Category : Science
ISBN : 9780387330150

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Glucose Sensing by Chris D. Geddes,Joseph R. Lakowicz Pdf

An essential reference for any laboratory working in the analytical fluorescence glucose sensing field. The increasing importance of these techniques is typified in one emerging area by developing non-invasive and continuous approaches for physiological glucose monitoring. This volume incorporates analytical fluorescence-based glucose sensing reviews, specialized enough to be attractive to professional researchers, yet appealing to a wider audience of scientists in related disciplines of fluorescence.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 502 pages
File Size : 43,9 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306470608

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Topics in Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Author : Joseph R. Lakowicz
Publisher : Unknown
Page : 564 pages
File Size : 50,7 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 1475787812

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Topics in Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 51,6 Mb
Release : 2002
Category : Electronic
ISBN : OCLC:723910791

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Topics in Fluorescence Spectroscopy by Anonim Pdf

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 432 pages
File Size : 55,5 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306470585

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Topics in Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

Author : Bernard Valeur,Jean-Claude Brochon
Publisher : Springer Science & Business Media
Page : 496 pages
File Size : 46,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642568534

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New Trends in Fluorescence Spectroscopy by Bernard Valeur,Jean-Claude Brochon Pdf

This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Author : Joseph R. Lacowicz
Publisher : Springer
Page : 310 pages
File Size : 51,8 Mb
Release : 2013-11-20
Category : Science
ISBN : 1475781946

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Protein Fluorescence by Joseph R. Lacowicz Pdf

The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with each other by resonance energy transfer (RET) decreasing the tyrosine emission. In this sense a protein is similar to a three-particle or mul- particle problem in quantum mechanics where the interaction between particles precludes an exact description of the system. In comparison, it has been easier to interpret the fluorescence data from labeled proteins because the fluorophore density and locations could be controlled so the probes did not interact with each other. From the origins of biochemical fluorescence in the 1950s with Prof- sor G. Weber until the mid-1980s, intrinsic protein fluorescence was more qualitative than quantitative. An early report in 1976 by A. Grindvald and I. Z. Steinberg described protein intensity decays to be multi-exponential. Attempts to resolve these decays into the contributions of individual tryp- phan residues were mostly unsuccessful due to the difficulties in resolving closely spaced lifetimes.

Author : Martin Hof,Rudolf Hutterer,V. Fidler
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 53,5 Mb
Release : 2004-12-08
Category : Science
ISBN : 354022338X

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Fluorescence Spectroscopy in Biology by Martin Hof,Rudolf Hutterer,V. Fidler Pdf

Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 544 pages
File Size : 52,9 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306470707

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Topics in Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970’s. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980’s. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one’s self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.

Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 313 pages
File Size : 42,5 Mb
Release : 2010-07-18
Category : Science
ISBN : 9780387236476

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Advanced Concepts in Fluorescence Sensing by Chris D. Geddes,Joseph R. Lakowicz Pdf

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 & 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 708 pages
File Size : 52,9 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475730616

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 462 pages
File Size : 41,8 Mb
Release : 1992-01-31
Category : Science
ISBN : 9780306438745

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Techniques by Joseph R. Lakowicz Pdf

In this inaugural volume of a new series, experts in the field help biochemists, analytical chemists, spectroscopists, biophysicists, and other specialists keep up with the latest techniques and technologies available in fluorescence spectroscopy.