A Beginner S Guide To Microarrays

Author : Eric M. Blalock
Publisher : Springer Science & Business Media
Page : 356 pages
File Size : 51,9 Mb
Release : 2011-06-27
Category : Science
ISBN : 9781441987600

Get Book

A Beginner’s Guide to Microarrays by Eric M. Blalock Pdf

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.

Author : Blalock Eric
Publisher : Unknown
Page : 368 pages
File Size : 42,9 Mb
Release : 2007-12-01
Category : Electronic
ISBN : 818128867X

Get Book

A Beginner'S Guide To Microarrays by Blalock Eric Pdf

Author : Helen Causton,John Quackenbush,Alvis Brazma
Publisher : John Wiley & Sons
Page : 176 pages
File Size : 55,7 Mb
Release : 2009-04-01
Category : Science
ISBN : 9781444311563

Get Book

Microarray Gene Expression Data Analysis by Helen Causton,John Quackenbush,Alvis Brazma Pdf

This guide covers aspects of designing microarray experiments and analysing the data generated, including information on some of the tools that are available from non-commercial sources. Concepts and principles underpinning gene expression analysis are emphasised and wherever possible, the mathematics has been simplified. The guide is intended for use by graduates and researchers in bioinformatics and the life sciences and is also suitable for statisticians who are interested in the approaches currently used to study gene expression. Microarrays are an automated way of carrying out thousands of experiments at once, and allows scientists to obtain huge amounts of information very quickly Short, concise text on this difficult topic area Clear illustrations throughout Written by well-known teachers in the subject Provides insight into how to analyse the data produced from microarrays

Author : Robert S. Matson
Publisher : CRC Press
Page : 216 pages
File Size : 54,7 Mb
Release : 2009-01-20
Category : Medical
ISBN : 9781420046663

Get Book

Microarray Methods and Protocols by Robert S. Matson Pdf

A Step-by-Step Guide to Present and Future Uses of Microarray TechnologyMicroarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts

Author : Steen Knudsen
Publisher : John Wiley & Sons
Page : 194 pages
File Size : 47,5 Mb
Release : 2005-03-04
Category : Science
ISBN : 9780471670261

Get Book

Guide to Analysis of DNA Microarray Data by Steen Knudsen Pdf

Written for biologists and medical researchers who don't have any special training in data analysis and statistics, Guide to Analysis of DNA Microarray Data, Second Edition begins where DNA array equipment leaves off: the image produced by the microarray. The text deals with the questions that arise starting at this point, providing an introduction to microarray technology, then moving on to image analysis, data analysis, cluster analysis, and beyond. With all chapters rewritten, updated, and expanded to include the latest generation of technology and methods, Guide to Analysis of DNA Microarray Data, Second Edition offers practitioners reliable information using concrete examples and a clear, comprehensible style. This Second Edition features entirely new chapters on: * Image analysis * Experiment design * Automated analysis, integrated analysis, and systems biology * Interpretation of results Intended for readers seeking practical applications, this text covers a broad spectrum of proven approaches in this rapidly growing technology. Additional features include further reading suggestions for each chapter, as well as a thorough review of available analysis software.

Author : Jang B. Rampal
Publisher : Springer Science & Business Media
Page : 923 pages
File Size : 43,5 Mb
Release : 2007-07-27
Category : Medical
ISBN : 9781588295897

Get Book

Microarrays by Jang B. Rampal Pdf

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

Author : Steen Knudsen
Publisher : John Wiley & Sons
Page : 148 pages
File Size : 54,9 Mb
Release : 2011-09-23
Category : Science
ISBN : 9780471461180

Get Book

A Biologist's Guide to Analysis of DNA Microarray Data by Steen Knudsen Pdf

A great introductory book that details reliable approaches to problems met in standard microarray data analyses. It provides examples of established approaches such as cluster analysis, function prediction, and principle component analysis. Discover real examples to illustrate the key concepts of data analysis. Written for those without any advanced background in math, statistics, or computer sciences, this book is essential for anyone interested in harnessing the immense potential of microarrays in biology and medicine.

Author : Steve Russell,Lisa A. Meadows,Roslin R. Russell
Publisher : Academic Press
Page : 460 pages
File Size : 53,8 Mb
Release : 2008-11-21
Category : Science
ISBN : 9780080919768

Get Book

Microarray Technology in Practice by Steve Russell,Lisa A. Meadows,Roslin R. Russell Pdf

Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays

Author : Dov Stekel
Publisher : Cambridge University Press
Page : 296 pages
File Size : 49,7 Mb
Release : 2003-09-08
Category : Medical
ISBN : 052152587X

Get Book

Microarray Bioinformatics by Dov Stekel Pdf

This book is a comprehensive guide to all of the mathematics, statistics and computing you will need to successfully operate DNA microarray experiments. It is written for researchers, clinicians, laboratory heads and managers, from both biology and bioinformatics backgrounds, who work with, or who intend to work with microarrays. The book covers all aspects of microarray bioinformatics, giving you the tools to design arrays and experiments, to analyze your data, and to share your results with your organisation or with the international community. There are chapters covering sequence databases, oligonucleotide design, experimental design, image processing, normalisation, identifying differentially expressed genes, clustering, classification and data standards. The book is based on the highly successful Microarray Bioinformatics course at Oxford University, and therefore is ideally suited for teaching the subject at postgraduate or professional level.

Author : Daniel H. Geschwind,Jeffrey P. Gregg
Publisher : MIT Press
Page : 366 pages
File Size : 40,9 Mb
Release : 2002
Category : DNA microarrays
ISBN : 0262072297

Get Book

Microarrays for the Neurosciences by Daniel H. Geschwind,Jeffrey P. Gregg Pdf

The effort to sequence the human genome has generated a new discipline, functional genomics, or the study of the relationship between the genetic code and its biologic potential. Gene expression studies are made possible not only by the decoding of the human genome, but by the development of new technologies. The preeminent technology in this area, DNA microarrays, is helping to revolutionize the field of neuroscience.

Author : Pierre Baldi,G. Wesley Hatfield
Publisher : Cambridge University Press
Page : 238 pages
File Size : 47,5 Mb
Release : 2011-04-28
Category : Science
ISBN : 1139437607

Get Book

DNA Microarrays and Gene Expression by Pierre Baldi,G. Wesley Hatfield Pdf

Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.

Author : Geoffrey J. McLachlan,Kim-Anh Do,Christophe Ambroise
Publisher : John Wiley & Sons
Page : 366 pages
File Size : 51,5 Mb
Release : 2005-02-18
Category : Mathematics
ISBN : 9780471726128

Get Book

Analyzing Microarray Gene Expression Data by Geoffrey J. McLachlan,Kim-Anh Do,Christophe Ambroise Pdf

A multi-discipline, hands-on guide to microarray analysis of biological processes Analyzing Microarray Gene Expression Data provides a comprehensive review of available methodologies for the analysis of data derived from the latest DNA microarray technologies. Designed for biostatisticians entering the field of microarray analysis as well as biologists seeking to more effectively analyze their own experimental data, the text features a unique interdisciplinary approach and a combined academic and practical perspective that offers readers the most complete and applied coverage of the subject matter to date. Following a basic overview of the biological and technical principles behind microarray experimentation, the text provides a look at some of the most effective tools and procedures for achieving optimum reliability and reproducibility of research results, including: An in-depth account of the detection of genes that are differentially expressed across a number of classes of tissues Extensive coverage of both cluster analysis and discriminant analysis of microarray data and the growing applications of both methodologies A model-based approach to cluster analysis, with emphasis on the use of the EMMIX-GENE procedure for the clustering of tissue samples The latest data cleaning and normalization procedures The uses of microarray expression data for providing important prognostic information on the outcome of disease

Author : Bertrand Jordan
Publisher : Springer Science & Business Media
Page : 158 pages
File Size : 50,8 Mb
Release : 2001-08-28
Category : Medical
ISBN : 3540415084

Get Book

DNA Microarrays by Bertrand Jordan Pdf

DNA Arrays for expression measurement: an historical perspective. (Bertrand R. Jordan). Expression profiling with cDNA microarrays: a user's perspective and guide. (Sean Grimmond and Andy Greenfield). cDNA microarrays on nylon membranes with enzyme colorimetric detection. (Konan Peck and Yuh-Pyng Sher). cDNA macroarrays and microarrays on nylon membranes with radioactive detection. (Beatrice Loriod, Geneviève Victorero and Catherine Nguyen). Oligonucleotide chips for expression analysis: principles and practical procedures. (Pierre Casellas, Annick Peleraux and Sylvaine Galiegue). Gene expression Data mining and analysis (Alvis Brazma, Alan Robinson and Jaak Vilo). Future trends in the use of DNA arrays for expression measurement. (Bertrand R. Jordan).

Author : Robert S. Matson
Publisher : CRC Press
Page : 260 pages
File Size : 40,5 Mb
Release : 2004-12-28
Category : Medical
ISBN : 9780203491133

Get Book

Applying Genomic and Proteomic Microarray Technology in Drug Discovery by Robert S. Matson Pdf

Microarrays play an increasingly significant role in drug discovery. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. The author addresses the drawbacks, helping

Author : Mark Schena
Publisher : Scion Publishing Ltd
Page : 289 pages
File Size : 46,9 Mb
Release : 2007-10-01
Category : Science
ISBN : 9781907904394

Get Book

DNA Microarrays by Mark Schena Pdf

DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. This book is a 'must have' manual for researchers in all fields of biology, medicine and agriculture.