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Author : D. M. Kemeny
Publisher : Pergamon
Page : 115 pages
File Size : 47,6 Mb
Release : 1991
Category : Medical
ISBN : 0080375081
"Written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay":- cover .
Author : John R. Crowther
Publisher : Springer Science & Business Media
Page : 426 pages
File Size : 40,6 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592590490
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Author : Brian Law
Publisher : CRC Press
Page : 242 pages
File Size : 40,9 Mb
Release : 2002-09-11
Category : Medical
ISBN : 9780203483497
Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.
Author : John R. Crowther
Publisher : Humana
Page : 0 pages
File Size : 41,6 Mb
Release : 2010-11-19
Category : Medical
ISBN : 1617378844
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author : John R. Crowther
Publisher : Springer Science & Business Media
Page : 231 pages
File Size : 42,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592595297
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Author : David Wild
Publisher : Newnes
Page : 1037 pages
File Size : 54,7 Mb
Release : 2013-01-21
Category : Science
ISBN : 9780080970387
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Author : J. Eryl Liddell,A. Cryer
Publisher : John Wiley & Sons
Page : 212 pages
File Size : 51,5 Mb
Release : 1991-08-26
Category : Medical
ISBN : 0471929050
Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Author : Robert Hnasko
Publisher : Humana
Page : 0 pages
File Size : 47,7 Mb
Release : 2015-07-10
Category : Medical
ISBN : 1493927418
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Author : D. M. Kemeny
Publisher : Pergamon
Page : 132 pages
File Size : 50,9 Mb
Release : 1991
Category : Enzyme-linked immunosorbent assay
ISBN : UOM:39015021858249
This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.
Author : Daniel Wan-Yui Chan
Publisher : Unknown
Page : 388 pages
File Size : 41,6 Mb
Release : 1992
Category : Medical
ISBN : WISC:89046269353
Immunoassay Automation: A Practical Guide describes automation of immunoassay from the practical viewpoint of the clinical laboratory. General introduction and evaluation sections demonstrate principles and practice. A comprehensive selection of available systems are detailed by experts, with a view towards popularity, technical advances, and operational efficiency. This laboratory guide is essential for practitioners in clinical chemistry laboratories, and will have lasting value in the evolution of automated systems. Focuses on automation of immunoassay for the clinical laboratory Emphasizes principles, method evaluation, and the systems approach Aids system selection by evaluation of technical, clinical, operational, and economical parameters Contains complete descriptions by experts on the latest automated immunoassay systems Based upon the editor's well-received workshops on automated immunoassay
Author : D. M. Kemeny,S. J. Challacombe
Publisher : John Wiley & Sons
Page : 384 pages
File Size : 48,6 Mb
Release : 1991-01-08
Category : Science
ISBN : 0471909823
This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.
Author : Elisa MacDonald
Publisher : Corwin Press
Page : 209 pages
File Size : 55,8 Mb
Release : 2013-03-13
Category : Education
ISBN : 9781452218830
All teams face hurdles. What distinguishes the skillful team leader from a less effective one is his or her approach in overcoming them. Whether you are a team leader or a trainer of team leaders, this book is an essential resource for you.Elisa MacDonald offers a skillful approach to team leadership rooted in values, mindset, intelligence, and skill. Readers will learn from reality-based examples illustrating common team hurdles in collaboration, shared leadership, goal setting and attainment, rigorous discourse, and continuous improvement.
Author : A. Voller
Publisher : Unknown
Page : 128 pages
File Size : 48,9 Mb
Release : 1979
Category : Electronic
ISBN : OCLC:28882779
Author : Alexander E. Kalyuzhny
Publisher : Springer Science & Business Media
Page : 323 pages
File Size : 44,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592599035
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
Author : James Ryan,Peter F. Mahoney,Ian Greaves,Gavin Bowyer
Publisher : Springer Science & Business Media
Page : 379 pages
File Size : 54,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781447102151
Conflict and Catastrophe Medicine - A Practical Guide provides a framework for use by health professionals visiting a resource-constrained environment. Encompassing problems brought about by local conflict or natural disasters, the book covers preparation, organisation, logistics, treatment of major trauma and medical emergencies, and the special problems of delivering medicine in a hostile environment. Conflict and Catastrophe Medicine - A Practical Guide comprehensively tackles: - self-preparation of health professionals to face a range of medical and related problems which occur in hostile and remote environments; - war and disaster medicine, covering acute management, rehabilitation, reconstruction and prevention; - bridging the fields of medicine, nursing, international relations, history, politics and economics. The book also touches on nutrition, infection, trauma, psychiatry and psychological medicine and training. James Ryan, Leonard Cheshire Professor of Conflict Recovery, Royal Free and University College Medical School, London, UK Peter F Mahoney, Consultant Anaesthetist and Honorary Senior Lecturer in Conflict Medicine, Leonard Cheshire Centre, Royal Free and University College Medical School, London, UK Ian Greaves, Lecturer in Conflict Medicine, Leonard Cheshire Centre, Royal Free and University College Medical School, London, UK Gavin Bowyer, Consultant in Orthopaedic Surgery, Southampton General Hospital, UK.