Advanced Chromatographic And Electromigration Methods In Biosciences

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Advanced Chromatographic and Electromigration Methods in BioSciences

I. Mikšík,F. Tagliaro,E. Tesarová,Z. Deyl
Advanced Chromatographic and Electromigration Methods in BioSciences Book PDF

Author : I. Mikšík,F. Tagliaro,E. Tesarová,Z. Deyl
Publisher : Elsevier
Page : 1125 pages
File Size : 51,8 Mb
Release : 1998-09-28
Category : Science
ISBN : 0080858686

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Advanced Chromatographic and Electromigration Methods in BioSciences by I. Mikšík,F. Tagliaro,E. Tesarová,Z. Deyl Pdf

This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted. Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Chromatography

Robert L. Wixom,Charles W. Gehrke
Chromatography Book PDF

Author : Robert L. Wixom,Charles W. Gehrke
Publisher : John Wiley & Sons
Page : 357 pages
File Size : 49,5 Mb
Release : 2011-01-31
Category : Science
ISBN : 9781118060292

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Chromatography by Robert L. Wixom,Charles W. Gehrke Pdf

Leading researchers discuss the past and present of chromatography More than one hundred years after Mikhail Tswett pioneered adsorption chromatography, his separation technique has developed into an important branch of scientific study. Providing a full portrait of the discipline, Chromatography: A Science of Discovery bridges the gap between early, twentieth-century chromatography and the cutting edge of today’s research. Featuring contributions from more than fifty award-winning chromatographers, Chromatography offers a multifaceted look at the development and maturation of this field into its current state, as well as its importance across various scientific endeavors. The coverage includes: Consideration of chromatography as a unified science rather than just a separation method Key breakthroughs, revolutions, and paradigm shifts in chromatography Profiles of Nobel laureates who used chromatography in their research, and the role it played Recent advances in column technology Chromatography’s contributions to the agricultural, space, biological/medical sciences; pharmaceutical science; and environmental, natural products, and chemical analysis Future trends in chromatography With numerous references and an engaging series of voices, Chromatography: A Science of Discovery offers a diverse look at an essential area of science. It is a unique and invaluable resource for researchers, students, and other interested readers who seek a broader understanding of this field.

Quantitation of Amino Acids and Amines by Chromatography

Ibolya Molnár-Perl
Quantitation of Amino Acids and Amines by Chromatography Book PDF

Author : Ibolya Molnár-Perl
Publisher : Elsevier
Page : 669 pages
File Size : 44,5 Mb
Release : 2005-06-27
Category : Science
ISBN : 9780080458458

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Quantitation of Amino Acids and Amines by Chromatography by Ibolya Molnár-Perl Pdf

Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC). The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines. * Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes * Compares advantages/disadvantages of various chromatographic techniques * Readers can undertake practical tasks using detailed protocols given in the book

Encyclopedia of Chromatography (Print)

Jack Cazes
Encyclopedia of Chromatography Print Book PDF

Author : Jack Cazes
Publisher : CRC Press
Page : 970 pages
File Size : 52,6 Mb
Release : 2001-06-29
Category : Science
ISBN : 0824705114

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Encyclopedia of Chromatography (Print) by Jack Cazes Pdf

This practical, single-volume source collects up-to-date information on chromatographic techniques and methodologies for the solution of analytical and preparative problems applicable across a broad spectrum of disciplines including biotechnology, pharmaceuticals, environmental sciences, polymers, food additives and nutrients, pathology, toxicology, fossil fuels, and nuclear chemistry. It highlights real-world applications, easy-to-read fundamentals of problem solving and material identification methods, and detailed references. Written by over 180 esteemed international authorities and containing over 300 chapters, 2600 works cited, and 1000 drawings, equations, tables, and photographs, the Encyclopedia of Chromatography covers high-performance liquid, thin-layer, gas, affinity, countercurrent, supercritical fluid, gel permeation, and size exclusion chromatographies as well as capillary electrophoresis, field-flow fractionation, hyphenated techniques, and more. PRINT/ONLINE PRICING OPTIONS AVAILABLE UPON REQUEST AT [email protected]

The Proteome Revisited

A. Stoyanov,M. Zhukov,Pier Giorgio Righetti
The Proteome Revisited Book PDF

Author : A. Stoyanov,M. Zhukov,Pier Giorgio Righetti
Publisher : Elsevier
Page : 410 pages
File Size : 48,9 Mb
Release : 2001-10-25
Category : Science
ISBN : 0080518966

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The Proteome Revisited by A. Stoyanov,M. Zhukov,Pier Giorgio Righetti Pdf

The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time.

Quantitative In Silico Chromatography

Toshihiko Hanai
Quantitative In Silico Chromatography Book PDF

Author : Toshihiko Hanai
Publisher : Royal Society of Chemistry
Page : 128 pages
File Size : 46,6 Mb
Release : 2014-05-07
Category : Science
ISBN : 9781782621508

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Quantitative In Silico Chromatography by Toshihiko Hanai Pdf

The coupling of mass spectrometry or nuclear magnetic resonance to chromatography has broadened the possibilities for determining organic reaction mechanisms. And while many results have been published reporting these, even more can be achieved through modern computational methods. Combining computational and theoretical techniques with advanced chromatographic methods offers a powerful tool for quantitatively determining molecular interactions . This book presents the possibilities for characterising biological applications by combining analytical and computational chemistries. Written by the author of “HPLC: A Practical Guide” (RSC, 1999), the book examines not only the behaviour of biological reactions per se, but also describes the behaviour of biological molecules in chromatography systems. Various software packages are reviewed, and most computations can be performed on a standard PC using accessible software. Consideration is given to a variety of chromatographic techniques and strategies for high-sensitivity detection are presented. The first book of its kind, it will inspire readers to explore the possibilities of combining these techniques in their own work, whether at an industrial or academic level.

Chromatography

E. Heftmann
Chromatography Book PDF

Author : E. Heftmann
Publisher : Elsevier
Page : 584 pages
File Size : 43,7 Mb
Release : 2004-04-16
Category : Science
ISBN : 9780080472249

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Chromatography by E. Heftmann Pdf

Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis. In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated. 1. Each chapter written by an authority 2. Thorough treatment of the theoretical basis of separation methods 3. Practical guide for performing analyses

Use of Humic Substances to Remediate Polluted Environments: From Theory to Practice

Irina V. Perminova,Kirk Hatfield,Norbert Hertkorn
Use of Humic Substances to Remediate Polluted Environments From Theory to Practice Book PDF

Author : Irina V. Perminova,Kirk Hatfield,Norbert Hertkorn
Publisher : Springer Science & Business Media
Page : 544 pages
File Size : 53,8 Mb
Release : 2005-03-24
Category : Science
ISBN : 1402032501

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Use of Humic Substances to Remediate Polluted Environments: From Theory to Practice by Irina V. Perminova,Kirk Hatfield,Norbert Hertkorn Pdf

Effective remediation of polluted environments is a priority in both Eastern and Western countries. In the U.S. and Europe, remediation costs generally exceed the net economic value of the land. As a result, scientists and engineers on both sides of the Atlantic have aggressively tried to develop novel technologies to meet regulatory standards at a fraction of the costs. In situ remediation shows considerable promise from both technical and economic perspectives. In situ technologies that deploy natural attenuating agents such as humic substances (HS) may be even more cost effective. Numerous studies have shown humics capable of altering both the chemical and the physical speciation of the ecotoxicants and in turn attenuate potential adverse environmental repercussions. Furthermore, the reserves of inexpensive humic materials are immense. Which suggests HS portend great promise as inexpensive amendments to mitigate the environmental impacts of ecotoxicants and as active agents in remediation. To elucidate emerging concepts of humics-based remediation technologies, we organized the NATO Advanced Research Workshop (ARW), entitled "Use of humates to remediate polluted environments: from theory to practice", held on September 23-29, 2002 in Zvenigorod, Russia (see the web-site http://www.mgumus.chem.msu.ru/arw).

Protein Liquid Chromatography

M. Kastner
Protein Liquid Chromatography Book PDF

Author : M. Kastner
Publisher : Elsevier
Page : 976 pages
File Size : 46,9 Mb
Release : 1999-12-13
Category : Science
ISBN : 9780080858692

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Protein Liquid Chromatography by M. Kastner Pdf

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Handbook of Thin-Layer Chromatography

Joseph Sherma,Bernard Fried
Handbook of Thin Layer Chromatography Book PDF

Author : Joseph Sherma,Bernard Fried
Publisher : CRC Press
Page : 1356 pages
File Size : 44,9 Mb
Release : 2003-04-18
Category : Science
ISBN : 9780824748661

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Handbook of Thin-Layer Chromatography by Joseph Sherma,Bernard Fried Pdf

In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

Monolithic Materials

F. Svec,T.B. Tennikova,Z. Deyl
Monolithic Materials Book PDF

Author : F. Svec,T.B. Tennikova,Z. Deyl
Publisher : Elsevier
Page : 800 pages
File Size : 50,6 Mb
Release : 2003-04-29
Category : Science
ISBN : 0080536972

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Monolithic Materials by F. Svec,T.B. Tennikova,Z. Deyl Pdf

During the past decade, monolithic materials in the shape of discs, stacked layers, rolled sheets, sponges, irregular chunks, tubes, and cylinders have all been successfully demonstrated. These formats were prepared from a wide variety of materials including natural polymers such as cellulose, synthetic polymers that involved porous styrene-, methacrylate-, and acrylamide-based polymers, and inorganic materials, mainly silica. Each approach is interesting from the point of view of both preparation and application. Although the current papers and patents concerned with monolithic separation media are quite numerous, the information is scattered throughout a vast number of journals. This book therefore fills the gap in the market for a comprehensive reference book on this subject. Monolithic materials concerns all of the current formats of monolithic materials and provides an integrated view of this novel format of separation media. Since the flow pattern in monolithic devices is different from that in packed beds, the hydrodynamics of the system and mass transport differ considerably from those derived for packed columns. Therefore, this book presents contributions concerned with both flow and mass transfer in the monolithic materials. A significant proportion of the book is devoted to the applications of monolithic materials. It also provides the reader with valuable information about the sources of the specific materials, their properties, and potential applications. · Monolithic materials are currently very popular within several scientific areas such as chromatography, optics, catalysis, diagnostics, genomics, proteomics, and microfluidics. · Provides valuable information about the sources of the specific materials, their properties, and potential applications. · Chapters written by leading experts in the area.

Emerging Technologies in Protein and Genomic Material Analysis

Gyorgy Marko-Varga,Peter Oroszlan
Emerging Technologies in Protein and Genomic Material Analysis Book PDF

Author : Gyorgy Marko-Varga,Peter Oroszlan
Publisher : Elsevier
Page : 294 pages
File Size : 49,6 Mb
Release : 2003-07-02
Category : Science
ISBN : 9780080530840

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Emerging Technologies in Protein and Genomic Material Analysis by Gyorgy Marko-Varga,Peter Oroszlan Pdf

It is widely recognized that analytical technologies and techniques are playing a pioneering role in a range of today's foremost challenging scientific endeavours, including especially biological and biomedical research. Worthy of mention, for example, are the role that high performance separation techniques played in mapping the human genome and the pioneering work done within mass spectrometry. It is also apparent that state-of-the-art pharmaceutical and biomedical research is the major driving force of the development of new analytical techniques. Advancements in genomics research has provided the opportunity for a call for new drug targets for new technologies, which has speeded up drug discovery and helped to counteract the trend towards inflation of R&D costs. This book has been designed to be a reference covering a wide range of protein and genomic material analysis techniques. Emerging developments are presented with applications where relevant, and biological examples are included. It was developed to meet the ever growing need for a comprehensive and balanced text on an analytical technique which has generated a tremendous amount of interest in recent years. In addition, this book also serves as a modern textbook for advanced undergraduate and graduate courses in various disciplines including chemistry, biology and pharmacy. Authors of the individual chapters are recognized champions of their individual research disciplines and also represent contemporary major research centres in this field. · Contains state-of-the-art knowledge of the field and detailed descriptions of new technologies · Provides examples of relevant applications and case studies · Contributing authors are leading scientists in their own respective research fields

Handbook of Forensic Drug Analysis

Fred Smith
Handbook of Forensic Drug Analysis Book PDF

Author : Fred Smith
Publisher : Elsevier
Page : 584 pages
File Size : 52,5 Mb
Release : 2004-12-31
Category : Law
ISBN : 0080472893

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Handbook of Forensic Drug Analysis by Fred Smith Pdf

The Handbook of Forensic Drug Analysis is a comprehensive chemical and analytic reference for the forensic analysis of illicit drugs. With chapters written by leading researchers in the field, the book provides in-depth, up-to-date methods and results of forensic drug analyses. This Handbook discusses various forms of the drug as well as the origin and nature of samples. It explains how to perform various tests, the use of best practices, and the analysis of results. Numerous forensic and chemical analytic techniques are covered including immunoassay, gas chromatography, and mass spectrometry. Topics range from the use of immunoassay technologies for drugs-of-abuse testing, to methods of forensic analysis for cannabis, hallucinogens, cocaine, opioids, and amphetamine. The book also looks at synthetic methods and law enforcement concerns regarding the manufacture of illicit drugs, with an emphasis on clandestine methamphetamine production. This Handbook should serve as a widely used reference for forensic scientists, toxicologists, pharmacologists, drug companies, and professionals working in toxicology testing labs, libraries, and poison control centers. It may also be used by chemists, physicians and those in legal and regulatory professions, and students of graduate courses in forensic science. Contributed to by leading scientists from around the world The only analysis book dedicated to illicit drugs of abuse Comprehensive coverage of sampling methods and various forms of analysis

Capillary Electrophoresis for Food Analysis

Richard A Frazier,Jennifer M Ames,H E Nursten
Capillary Electrophoresis for Food Analysis Book PDF

Author : Richard A Frazier,Jennifer M Ames,H E Nursten
Publisher : Royal Society of Chemistry
Page : 143 pages
File Size : 44,6 Mb
Release : 2007-10-31
Category : Technology & Engineering
ISBN : 9781847550316

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Capillary Electrophoresis for Food Analysis by Richard A Frazier,Jennifer M Ames,H E Nursten Pdf

Since its inception in the early 1980s, capillary electrophoresis (CE) offers a great deal of flexibility as a modern analytical technique, and has found applications within many fields of analysis, particularly pharmaceutical science and biochemistry. Until now, food analysts have had difficulties in adopting the technique due to the lack of written guidance. Capillary Electrophoresis for Food Analysis: Method Development provides basic information and the support needed to enable food analysts to utilise the technique for the development of new separation methods. Designed specifically for the needs of food analysts, the book takes the reader step by step through the process of developing and troubleshooting CE methods. Worked examples are included to make it ideal as a laboratory companion as well as a library reference source.

Mechanochromic Fluorescent Materials

Jiarui Xu,Zhenguo Chi
Mechanochromic Fluorescent Materials Book PDF

Author : Jiarui Xu,Zhenguo Chi
Publisher : Royal Society of Chemistry
Page : 274 pages
File Size : 48,5 Mb
Release : 2014-05-08
Category : Technology & Engineering
ISBN : 9781782621539

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Mechanochromic Fluorescent Materials by Jiarui Xu,Zhenguo Chi Pdf

Mechanochromic fluorescent (or mechanofluorochromic) materials change their emission colours (spectra) when an appropriate external mechanical force stimulus is applied. This is an important group of materials with a huge range of applications, including use in sensors, memory chips, security inks, and light devices. Mechanochromic Fluorescent Materials introduces the reader to the concept of mechanofluorochromism and the variety of applications of this group of materials. Prominent international figures in mechanofluorochromism consider the innovative research in this field over the last ten years. Chapters provide in depth coverage of most reported mechanofluorochromic systems, including organic and organic-inorganic complexes; polymer and polymer composites; and aggregation-induced emission. This book is aimed to inform all students and researchers with an interest in mechanofluorochromism, and to help researchers identify and synthesize more of these materials, and develop the study and application of mechanofluorochromic materials.