Chromatography

Author : Elsa Lundanes,Léon Reubsaet,Tyge Greibrokk
Publisher : John Wiley & Sons
Page : 228 pages
File Size : 48,7 Mb
Release : 2013-12-04
Category : Science
ISBN : 9783527336203

Get Book

Chromatography by Elsa Lundanes,Léon Reubsaet,Tyge Greibrokk Pdf

Finally a book on chromatography which is easy to grasp for undergraduates and technicians; covers the area in sufficient depth while still being concise. The book includes all recent technology advances and has core textbook features further improving the learning experience. This book is the perfect introduction into a methodology which is the underlying principle of the vast majority of separation methods worldwide. Everyone working in a lab environment must be familiar with the basis of these technologies and Tyge Greibrokk, Elsa Lundanes and Leon Reubsaet succeed in delivering a text which is easy to read for undergraduates and laboratory technicians, and covers the area in sufficient depth while still being concise. The book includes all recent technology advances and has core textbook features further improving the learning experience. Importantly, the text does not only cover all major modern chromatography technology (thin layer, gas, high pressure liquid, and supercritical fluid chromatography) but also related methods, in particular electrophoretic technologies.

Author : H. Schmidt-Traub
Publisher : John Wiley & Sons
Page : 485 pages
File Size : 51,8 Mb
Release : 2006-03-06
Category : Technology & Engineering
ISBN : 9783527605903

Get Book

Preparative Chromatography by H. Schmidt-Traub Pdf

This interdisciplinary approach combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. The book covers recent developments in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on solid materials and packed columns process concepts. Final chapters on modeling and determination of model parameters, the design and optimization of preparative chromatographic processes and chromatographic reactors allow for the optimum selection of chromatographic systems. Essential for chemists and engineers working in the chemicals and pharmaceutical industries as well as for food technologies, due to the interdisciplinary nature of these processes.

Author : C. F. Poole
Publisher : Elsevier
Page : 952 pages
File Size : 55,9 Mb
Release : 2003
Category : Science
ISBN : 0444501991

Get Book

The Essence of Chromatography by C. F. Poole Pdf

The Essence of Chromatography presents a comprehensive survey of modern chromatography and is intended as a suitable text for graduate level courses in the separation sciences and as a self-study guide for professional chromatographers wishing to refresh their background in this rapidly expanding field.This title is an effective replacement for Chromatography Today, written by the same author with Salwa K. Poole, which is considered to be one of the definitive chromatographic texts of the last decade. Its format is modular, with extensive cross-references to permit rapid location of re.

Author : Georges Guiochon,Dean G. Shirazi,Attila Felinger
Publisher : Academic Press
Page : 991 pages
File Size : 42,7 Mb
Release : 2006-03-21
Category : Medical
ISBN : 9780123705372

Get Book

Fundamentals of Preparative and Nonlinear Chromatography by Georges Guiochon,Dean G. Shirazi,Attila Felinger Pdf

The second edition of Fundamentals of Preparative and Nonlinear Chromatography is devoted to the fundamentals of a new process of purification or extraction of chemicals or proteins widely used in the pharmaceutical industry and in preparative chromatography. This process permits the preparation of extremely pure compounds satisfying the requests of the US Food and Drug Administration. The book describes the fundamentals of thermodynamics, mass transfer kinetics, and flow through porous media that are relevant to chromatography. It presents the models used in chromatography and their solutions, discusses the applications made, describes the different processes used, their numerous applications, and the methods of optimization of the experimental conditions of this process.

Author : M. Ranny
Publisher : Springer Science & Business Media
Page : 206 pages
File Size : 53,9 Mb
Release : 1987-06-30
Category : Science
ISBN : 902771973X

Get Book

Thin-Layer Chromatography with Flame Ionization Detection by M. Ranny Pdf

Thin-layer chromatography (TLC) has become a common and much favoured separation technique in laboratories in widely varied fields in recent years. Much of the credit for the introduction of this technique into analytical practice at the l 2 end of the 1950s is due to E. Stahl • • This method is simple and is characterized by high separation ability and sufficient sensitivity3; however, some analysts feel that it has passed the peak in its development and will gradually be replaced by the more modem high-performance liquid chromatography (HPLC). This is undoubtedly a very important analytical technique utilizing the specific separa tion properties of a large number of sorbents and the possibility of regulating 4 the flow-rate of the mobile phase by adjusting the pressure • Standardization of the experimental conditions is simpler in HPLC than in TLC, where the activity of the sorbent and flow-rate of the eitlent in the thin layer depend markedly on the relative humidity of the laboratory atmosphere and on the composition of the gaseous phase in the elution chamber. In addition, systems for quantitative detection of the separated ~ones are better developed for HPLC than for classical TLC, where, until recently, cumbersome and often even insufficiently reproducible chemical or gravimetric analysis of the extracts of scraped-off spots or densitometry of the separated zones, located first by pyrolysis or reaction s with suitable detection agents, were the predominant determination methods .

Author : James M. Miller
Publisher : John Wiley & Sons
Page : 518 pages
File Size : 54,5 Mb
Release : 2005-12-16
Category : Science
ISBN : 9780471980599

Get Book

Chromatography by James M. Miller Pdf

The first edition of Chromatography: Concepts and Contrasts, published in 1988, was one of the first books to discuss all the different types of chromatography under one cover. The second edition continues with these principles but has been updated to include new chapters on sampling and sample preparation, capillary electrophoresis and capillary electrochromatography (CEC), chromatography with mass spec detection, and industrial and governmental practices in regulated industries. Covers extraction, solid phase extraction (SPE), and solid phase microextraction (SPME), and introduces mass spectrometry Updated with the latest techniques in chromatography Discusses both liquid chromatography (LC)and gas chromatography(GC)

Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Page : 831 pages
File Size : 49,7 Mb
Release : 2011-09-20
Category : Science
ISBN : 9781118210390

Get Book

Introduction to Modern Liquid Chromatography by Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan Pdf

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Author : Giorgio Carta,Alois Jungbauer
Publisher : John Wiley & Sons
Page : 440 pages
File Size : 47,5 Mb
Release : 2020-06-02
Category : Science
ISBN : 9783527346660

Get Book

Protein Chromatography by Giorgio Carta,Alois Jungbauer Pdf

An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.

Author : Andrea Weston,Phyllis R. Brown
Publisher : Elsevier
Page : 280 pages
File Size : 54,5 Mb
Release : 1997-07-21
Category : Science
ISBN : 0080534163

Get Book

High Performance Liquid Chromatography & Capillary Electrophoresis by Andrea Weston,Phyllis R. Brown Pdf

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature

Author : Eugene W. Hammond
Publisher : CRC Press
Page : 206 pages
File Size : 50,9 Mb
Release : 1993-02-09
Category : Science
ISBN : 0849342554

Get Book

Chromatography for the Analysis of Lipids by Eugene W. Hammond Pdf

Chromatography for the Analysis of Lipids focuses on the three main chromatographic techniques for analyzing lipids: thin layer (TLC), gas liquid (GLC), and high performance liquid chromatography (HPLC). It also covers supercritical fluid chromatography (SFC). Applications for each chromatographic technique are discussed with emphasis on their quantitative aspects and suitability for use, and variations in technique are covered in detail. Figures are used throughout the book to illustrate typical results, which shows end points that can be compared in your own laboratory. This important technical book will be an excellent workbench companion for chromatographers, food scientists, lipid chemists, analytical chemists, and others who use chromatographic techniques for lipid analysis.

Author : Łukasz Komsta,Yvan Vander Heyden,Joseph Sherma
Publisher : CRC Press
Page : 506 pages
File Size : 54,7 Mb
Release : 2018-02-02
Category : Science
ISBN : 9781498772549

Get Book

Chemometrics in Chromatography by Łukasz Komsta,Yvan Vander Heyden,Joseph Sherma Pdf

Chemometrics uses advanced mathematical and statistical algorithms to provide maximum chemical information by analyzing chemical data, and obtain knowledge of chemical systems. Chemometrics significantly extends the possibilities of chromatography and with the technological advances of the personal computer and continuous development of open-source software, many laboratories are interested in incorporating chemometrics into their chromatographic methods. This book is an up-to-date reference that presents the most important information about each area of chemometrics used in chromatography, demonstrating its effective use when applied to a chromatographic separation.

Author : Sadao Mori,Howard G. Barth
Publisher : Springer Science & Business Media
Page : 236 pages
File Size : 42,7 Mb
Release : 2013-03-09
Category : Technology & Engineering
ISBN : 9783662039106

Get Book

Size Exclusion Chromatography by Sadao Mori,Howard G. Barth Pdf

Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

Author : Anonim
Publisher : Krishna Prakashan Media
Page : 412 pages
File Size : 50,7 Mb
Release : 2024-06-28
Category : Electronic
ISBN : 8210379456XXX

Get Book

Chromatography by Anonim Pdf

Author : Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Publisher : Elsevier
Page : 808 pages
File Size : 43,8 Mb
Release : 2017-06-22
Category : Science
ISBN : 9780128093450

Get Book

Liquid Chromatography by Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola Pdf

Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Author : Kumar D. Mukherjee,Nikolaus Weber
Publisher : CRC Press
Page : 546 pages
File Size : 45,8 Mb
Release : 1993-05-06
Category : Science
ISBN : 0849330394

Get Book

CRC Handbook of Chromatography by Kumar D. Mukherjee,Nikolaus Weber Pdf

Handbook of Chromatography: Analysis of Lipids provides a valuable review of state-of-the-art applications of chromatographic techniques (TLC, GC, HPLC) and other analytical techniques. Much of this volume is devoted to applications of HPLC (including supercritical fluid chromatography) in the analysis of lipids such as fatty acids, oxygenated fatty acids, enantiomeric acyl- and alkylglycerols, and lipoproteins. The handbook also provides extensive coverage of applications of combinations of various chromatographic techniques used in the analysis of ozonides, anacardic acids, glycerophospholipids, products of lipolysis, artifacts and contaminants in edible fats, acylated proteins, non-caloric lipids, lipophilic vitamins, acyl-Coenzyme A thioesters, dolichols, mycolic acids, technical fats and fat products, and liposomes. Handbook of Chromatography: Analysis of Lipids will be a useful reference for oil chemists, biochemists, fat science technologists, and other scientists involved in lipid research.