Analytical Techniques In Biosciences

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Analytical Techniques in Biosciences

Chukwuebuka Egbuna,Kingsley C. Patrick-Iwuanyanwu,Muhammad Ajmal Shah,Jonathan C. Ifemeje,Azhar Rasul
Analytical Techniques in Biosciences Book PDF

Author : Chukwuebuka Egbuna,Kingsley C. Patrick-Iwuanyanwu,Muhammad Ajmal Shah,Jonathan C. Ifemeje,Azhar Rasul
Publisher : Academic Press
Page : 302 pages
File Size : 45,7 Mb
Release : 2021-10-21
Category : Science
ISBN : 9780128227992

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Analytical Techniques in Biosciences by Chukwuebuka Egbuna,Kingsley C. Patrick-Iwuanyanwu,Muhammad Ajmal Shah,Jonathan C. Ifemeje,Azhar Rasul Pdf

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences

Bioanalytics

Friedrich Lottspeich,Joachim W. Engels
Bioanalytics Book PDF

Author : Friedrich Lottspeich,Joachim W. Engels
Publisher : John Wiley & Sons
Page : 450 pages
File Size : 48,9 Mb
Release : 2018-03-08
Category : Science
ISBN : 9783527694440

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Bioanalytics by Friedrich Lottspeich,Joachim W. Engels Pdf

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Bioanalytics

Friedrich Lottspeich,Joachim W. Engels
Bioanalytics Book PDF

Author : Friedrich Lottspeich,Joachim W. Engels
Publisher : John Wiley & Sons
Page : 1151 pages
File Size : 45,8 Mb
Release : 2018-05-29
Category : Science
ISBN : 9783527339198

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Bioanalytics by Friedrich Lottspeich,Joachim W. Engels Pdf

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Analytical Techniques in Biochemistry and Molecular Biology

Rajan Katoch
Analytical Techniques in Biochemistry and Molecular Biology Book PDF

Author : Rajan Katoch
Publisher : Springer Science & Business Media
Page : 441 pages
File Size : 53,6 Mb
Release : 2011-07-19
Category : Science
ISBN : 1441997857

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Analytical Techniques in Biochemistry and Molecular Biology by Rajan Katoch Pdf

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Bioanalytical Techniques

Abhilasha Shourie
Bioanalytical Techniques Book PDF

Author : Abhilasha Shourie
Publisher : The Energy and Resources Institute (TERI)
Page : 424 pages
File Size : 50,6 Mb
Release : 2005-01-01
Category : Science
ISBN : 9788179935293

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Bioanalytical Techniques by Abhilasha Shourie Pdf

Bioanalytical Techniques form an integral part of applied biology and biomedical sciences. The various principles of bioanalytical techniques used in biomedical sciences, environmental studies, life sciences, pharmaceutical analysis, molecular biology, and biotechnological research are comprehensively discussed in this book. Analytical instrumentation is also explained in as concise a manner as possible. Microscopy, centrifugation, chromatography, electrophoresis, spectroscopy, and radioisotope and immunodiagnostic techniques are the main topics focussed in this book. Techniques in molecular biology and recombinant DNA technology have also been described in detail.

Analytical Biotechnology

Thomas G.M. Schalkhammer
Analytical Biotechnology Book PDF

Author : Thomas G.M. Schalkhammer
Publisher : Birkhäuser
Page : 340 pages
File Size : 49,8 Mb
Release : 2012-11-28
Category : Science
ISBN : 9783034881012

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Analytical Biotechnology by Thomas G.M. Schalkhammer Pdf

Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Analytical Techniques in Biotechnology

Suzy Hill
Analytical Techniques in Biotechnology Book PDF

Author : Suzy Hill
Publisher : Unknown
Page : 0 pages
File Size : 45,9 Mb
Release : 2016-05-26
Category : Electronic
ISBN : 1682862380

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Analytical Techniques in Biotechnology by Suzy Hill Pdf

Biotechnology is a rapidly progressing field of science and technology. It integrates principles and applications from diverse fields such as genomics, immunology, and many more. This book strives to equip the reader with thorough knowledge about various techniques of applied biotechnology, its applications in the areas of medicine, agriculture, environment, etc., along with details about the latest equipment and analytical tools which would help the reader gain an in-depth perspective about the subject. This book elucidates new techniques and their applications in a multidisciplinary approach and is apt for students of biotechnology and associated disciplines of study.

Analytical Biotechnology

Thomas G.M. Schalkhammer
Analytical Biotechnology Book PDF

Author : Thomas G.M. Schalkhammer
Publisher : Springer Science & Business Media
Page : 356 pages
File Size : 54,7 Mb
Release : 2002-09-23
Category : Science
ISBN : 3764365900

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Analytical Biotechnology by Thomas G.M. Schalkhammer Pdf

Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Analytical Techniques in Forensic Science

Rosalind Wolstenholme,Sue Jickells,Shari Forbes
Analytical Techniques in Forensic Science Book PDF

Author : Rosalind Wolstenholme,Sue Jickells,Shari Forbes
Publisher : John Wiley & Sons
Page : 464 pages
File Size : 51,5 Mb
Release : 2021-01-26
Category : Medical
ISBN : 9781119978282

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Analytical Techniques in Forensic Science by Rosalind Wolstenholme,Sue Jickells,Shari Forbes Pdf

An in-depth text that explores the interface between analytical chemistry and trace evidence Analytical Techniques in Forensic Science is a comprehensive guide written in accessible terms that examines the interface between analytical chemistry and trace evidence in forensic science. With contributions from noted experts on the topic, the text features a detailed introduction analysis in forensic science and then subsequent chapters explore the laboratory techniques grouped by shared operating principles. For each technique, the authors incorporate specific theory, application to forensic analytics, interpretation, forensic specific developments, and illustrative case studies. Forensic techniques covered include UV-Vis and vibrational spectroscopy, mass spectrometry and gas and liquid chromatography. The applications reviewed include evidence types such as fibers, paint, drugs and explosives. The authors highlight data collection, subsequent analysis, what information has been obtained and what this means in the context of a case. The text shows how analytical chemistry and trace evidence can problem solve the nature of much of forensic analysis. This important text: Puts the focus on trace evidence and analytical science Contains case studies that illustrate theory in practice Includes contributions from experts on the topics of instrumentation, theory, and case examples Explores novel and future applications for analytical techniques Written for undergraduate and graduate students in forensic chemistry and forensic practitioners and researchers, Analytical Techniques in Forensic Science offers a text that bridges the gap between introductory textbooks and professional level literature.

Advances in Biological Science Research

Surya Nandan Meena,Milind Naik
Advances in Biological Science Research Book PDF

Author : Surya Nandan Meena,Milind Naik
Publisher : Academic Press
Page : 580 pages
File Size : 47,6 Mb
Release : 2019-05-17
Category : Science
ISBN : 9780128174982

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Advances in Biological Science Research by Surya Nandan Meena,Milind Naik Pdf

Advances in Biological Science Research: A Practical Approach provides discussions on diverse research topics and methods in the biological sciences in a single platform. This book provides the latest technologies, advanced methods, and untapped research areas involved in diverse fields of biological science research such as bioinformatics, proteomics, microbiology, medicinal chemistry, and marine science. Each chapter is written by renowned researchers in their respective fields of biosciences and includes future advancements in life science research. Discusses various research topics and methods in the biological sciences in a single platform Comprises the latest updates in advanced research techniques, protocols, and methods in biological sciences Incorporates the fundamentals, advanced instruments, and applications of life science experiments Offers troubleshooting for many common problems faced while performing research experiments

Principles and Techniques of Biochemistry and Molecular Biology

Keith Wilson,John Walker
Principles and Techniques of Biochemistry and Molecular Biology Book PDF

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 53,8 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

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Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Analytical Molecular Biology

Helen Parkes,V (Ginny) Saunders
Analytical Molecular Biology Book PDF

Author : Helen Parkes,V (Ginny) Saunders
Publisher : Royal Society of Chemistry
Page : 209 pages
File Size : 47,7 Mb
Release : 2007-10-31
Category : Science
ISBN : 9781847559296

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Analytical Molecular Biology by Helen Parkes,V (Ginny) Saunders Pdf

In spite of the wide variety and complexity of biological materials, nucleic acids are ubiquitous. DNA is becoming the bioanalyte of choice due to the vast amount of information embedded in its sequence, its robust chemical nature and the range of highly sensitive analytical techniques that have been developed. The results of such analyses can have an important impact on our society both commercially and in terms of the quality of life. Absolute confidence in the data generated is therefore of the utmost importance. This book, produced by LGC as part of the VAM (Valid Analytical Measurement) Programme, introduces the issues of validation and quality to the bioanalytical community, specifically addressing DNA-based analyses. It aims to raise awareness of the factors that can influence the validity of DNA analysis and the production of quality data. Emphasis is placed on VAM principles, as well as additional challenges that are associated with the analysis of real samples, for example, complex food matrices or forensic samples that have been subjected to environmental insult. Information is collated from a variety of sources including literature, discussions and LGC research, and offers constructive advice where possible.

Cluster and Classification Techniques for the Biosciences

Alan H. Fielding
Cluster and Classification Techniques for the Biosciences Book PDF

Author : Alan H. Fielding
Publisher : Cambridge University Press
Page : 4 pages
File Size : 44,9 Mb
Release : 2006-12-14
Category : Medical
ISBN : 9781139460064

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Cluster and Classification Techniques for the Biosciences by Alan H. Fielding Pdf

Advances in experimental methods have resulted in the generation of enormous volumes of data across the life sciences. Hence clustering and classification techniques that were once predominantly the domain of ecologists are now being used more widely. This 2006 book provides an overview of these important data analysis methods, from long-established statistical methods to more recent machine learning techniques. It aims to provide a framework that will enable the reader to recognise the assumptions and constraints that are implicit in all such techniques. Important generic issues are discussed first and then the major families of algorithms are described. Throughout the focus is on explanation and understanding and readers are directed to other resources that provide additional mathematical rigour when it is required. Examples taken from across the whole of biology, including bioinformatics, are provided throughout the book to illustrate the key concepts and each technique's potential.

Instrumental Analysis in the Biological Sciences

M. H. Gordon
Instrumental Analysis in the Biological Sciences Book PDF

Author : M. H. Gordon
Publisher : Springer Science & Business Media
Page : 244 pages
File Size : 48,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468415216

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Instrumental Analysis in the Biological Sciences by M. H. Gordon Pdf

Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biological materials that is not equipped with an extensive range of instrumentation. Recent years have also seen a dramatic improvement in the ease with which such instruments can be used, and the quality and quantity of the analytical data that they can produce. This is due in no sm all part to the ubiquitous use of microprocessors and computers for instrumental control. However, under these circumstances there is areal danger of the analyst adopting a 'black box' mentality and not treating the analytical data produced in accordance with the limitations that may be inherent in the method used. Such a problem can only be overcome if the operator is fully aware of both the theoretical and instrumental constraints relevant to the technique in question. As the complexity and sheer volume of material in undergraduate courses increases, there is a tendency to reduce the amount of fundamental material that is taught prior to embarking on the more applied aspects. This is nowhere more apparent than in the teaching of instrumental techniques of analysis.

Calculations for Molecular Biology and Biotechnology

Frank H. Stephenson
Calculations for Molecular Biology and Biotechnology Book PDF

Author : Frank H. Stephenson
Publisher : Academic Press
Page : 460 pages
File Size : 45,6 Mb
Release : 2010-07-30
Category : Science
ISBN : 012375691X

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Calculations for Molecular Biology and Biotechnology by Frank H. Stephenson Pdf

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts