Animal Cell Culture

Author : R. Ian Freshney
Publisher : Wiley-Liss
Page : 510 pages
File Size : 43,6 Mb
Release : 1993-12-29
Category : Science
ISBN : 0471589667

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Culture of Animal Cells by R. Ian Freshney Pdf

This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Author : Michael Butler
Publisher : Garland Science
Page : 299 pages
File Size : 42,9 Mb
Release : 2004-08-02
Category : Medical
ISBN : 9781135325374

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Animal Cell Culture and Technology by Michael Butler Pdf

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Author : John M. Davis
Publisher : John Wiley & Sons
Page : 389 pages
File Size : 52,9 Mb
Release : 2011-03-21
Category : Science
ISBN : 9780470666586

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Animal Cell Culture by John M. Davis Pdf

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Author : Martin Clynes
Publisher : Springer Science & Business Media
Page : 624 pages
File Size : 52,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642804120

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Animal Cell Culture Techniques by Martin Clynes Pdf

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Author : Glyn N. Stacey,John Davis
Publisher : John Wiley & Sons
Page : 696 pages
File Size : 44,7 Mb
Release : 2007-06-29
Category : Science
ISBN : 0470723785

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Medicines from Animal Cell Culture by Glyn N. Stacey,John Davis Pdf

Medicines from Animal Cell Culture focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells. Medicines from Animal Cell Culture: Provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterisation of both the cells and the derived products Describes the preparation of stem cells and others for use in cell-based therapies – an area of burgeoning research Includes experimental examples to indicate expected results Covers regulatory issues from the UK, the EU and the USA and reviews how these are developing around the world Addresses the key issues of standardisation and validation with chapters on GLP and GMP for cell culture processes Delivering insight into the exciting world of biological medicines and directions for further investigation into specific topics, Medicines from Animal Cell Culture is an essential resource for researchers and technicians at all levels using cell culture within the pharmaceutical, biotechnology and biomedical industries. It is of value to laboratory managers in these industries and to all those interested in this topic alike.

Author : A Wilson Aruni,P Ramadass
Publisher : MJP Publisher
Page : 219 pages
File Size : 42,9 Mb
Release : 2019-06-10
Category : Fiction
ISBN : 8210379456XXX

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Animal Tissue Culture by A Wilson Aruni,P Ramadass Pdf

Cell and tissues culture has been one of the first and foremost techniques paving for recent cutting-edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. It has played a substantial role in the developments of health care and prophylactics industries, thus serving the mankind. It has made the dream of producing cost-effective prophylactics, diagnostics and therapeutics come true and affordable. In the recent past, with the explosion of knowledge in the field of biotechnology, intensive research in being carried out, where undergraduate and post-graduate courses are being offered in this field. Even through more emphasis is being given to theory, a dearth of practical knowledge is lacking due to paucity of established tissue culture facilities.

Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 763 pages
File Size : 55,7 Mb
Release : 2014-11-28
Category : Medical
ISBN : 9783319103204

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Animal Cell Culture by Mohamed Al-Rubeai Pdf

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Author : Ralf Pörtner
Publisher : Humana
Page : 0 pages
File Size : 44,8 Mb
Release : 2016-08-23
Category : Science
ISBN : 149396318X

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Animal Cell Biotechnology by Ralf Pörtner Pdf

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Author : D. C. Darling,S. J. Morgan
Publisher : Wiley-Blackwell
Page : 186 pages
File Size : 48,7 Mb
Release : 1994
Category : Medical
ISBN : UOM:39015033318224

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Animal Cells: Culture and Media by D. C. Darling,S. J. Morgan Pdf

This reference guide covers the fundamentals of animal cell cultures. It includes details of culture media, supplements, culture vessels, standard cell lines, passaging, cell separation techniques, cryopreservation, transfection, cell cloning and the creation of cell lines.

Author : Anonim
Publisher : Universities Press
Page : 248 pages
File Size : 53,7 Mb
Release : 2024-06-15
Category : Electronic
ISBN : 9788173717192

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Principles and Practice of Animal Tissue Culture (Second Edition) by Anonim Pdf

Author : Sara J. Morgan,David C. Darling
Publisher : Taylor & Francis
Page : 184 pages
File Size : 54,7 Mb
Release : 1993
Category : Medical
ISBN : UOM:39015029117788

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Animal Cell Culture by Sara J. Morgan,David C. Darling Pdf

This volume is intended as comprehensive introduction to current techniques in animal cell culture and the equipment needed to set up a tissue culture facility. The emphasis throughout, is on the practical aspects of cell culture required by advanced undergraduate students and postgraduates. It is intended for 2nd and 3rd year undergraduates in the biological sciences, postgraduates, research technicians and all who are new to working with tissue culture. Experienced workers should also find the book useful.

Author : John M. Davis
Publisher : John Wiley & Sons
Page : 389 pages
File Size : 48,7 Mb
Release : 2011-03-16
Category : Science
ISBN : 9780470975633

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Animal Cell Culture by John M. Davis Pdf

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Author : Jeffrey W. Pollard,John M. Walker
Publisher : Springer Science & Business Media
Page : 477 pages
File Size : 40,6 Mb
Release : 1997
Category : Animals
ISBN : 9780896034419

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Basic Cell Culture Protocols by Jeffrey W. Pollard,John M. Walker Pdf

Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Author : Chester S. Ho
Publisher : Butterworth-Heinemann
Page : 512 pages
File Size : 53,5 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483289472

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Animal Cell Bioreactors by Chester S. Ho Pdf

Animal Cell Bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. It addresses engineering aspects such as mass transfer, instrumentation, and control ensuring successful design and operation of animal cell bioreactors. The goal is to provide a comprehensive analysis and review in the advancement of the bioreactor systems for large-scale animal cell cultures. The book is organized into four parts. Part I traces the historical development of animal cell biotechnology. It presents examples of work in progress that seeks to make animal cell biotechnology processes as productive on a cost per unit of product basis as that achieved by other microbial systems. Part II includes chapters dealing with the implications of cell biology in animal cell biotechnology; protein-bound oligosaccharides and their structures; the development of serum-free media and its use in the production of biologically active substances; and the metabolism of mammalian cells. Part III focuses on animal cell cultivation, covering topics such as the fixed bed immobilized culture; three-dimensional microcarriers; and hydrodynamic phenomena in microcarrier cultures. Part IV discusses the design, operation, and control of animal cell bioreactors.

Author : Hansjörg Hauser,Roland Wagner
Publisher : Walter de Gruyter GmbH & Co KG
Page : 717 pages
File Size : 42,7 Mb
Release : 2014-11-10
Category : Science
ISBN : 9783110278965

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Animal Cell Biotechnology by Hansjörg Hauser,Roland Wagner Pdf

This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.