Animal Cell Biotechnology

Author : Ralf Pörtner
Publisher : Humana
Page : 0 pages
File Size : 54,8 Mb
Release : 2016-08-23
Category : Science
ISBN : 149396318X

Get Book

Animal Cell Biotechnology by Ralf Pörtner Pdf

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Author : Anonim
Publisher : Elsevier
Page : 384 pages
File Size : 40,8 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080925998

Get Book

Animal Cell Biotechnology by Anonim Pdf

There has been a dramatic increase in the perception of the value of animal cell biotechnology to the research and manufacturing communities in recent years. This volume seeks to keep the reader up-to-date with this progress. This sixth and final volume in the series concentrates on the biology of animal cells in culture, giving special attention to the relationship between biology and the ability to use such cells productively. As the search continues for greater productivity, there is a need to understand the switches within cells that control expression. Additional abilities to manipulate those switches in a controllable manner are also required. In the last five years, considerable progress has been made in the elucidation of the mechanisms for cell signaling and control of gene expression. The 13 chapters of this volume are devoted to these subjects and to techniques in areas of particular concern in manufacturing circles. The achievements in the field to date are described in this book, which, together with its five companion volumes in the series, will provide a building block for the future development of animal cell biotechnology.

Author : Ralf Pörtner
Publisher : Springer Science & Business Media
Page : 1035 pages
File Size : 46,8 Mb
Release : 2007-04-05
Category : Science
ISBN : 9781588296603

Get Book

Animal Cell Biotechnology by Ralf Pörtner Pdf

The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

Author : Hansjörg Hauser,Roland Wagner
Publisher : Walter de Gruyter GmbH & Co KG
Page : 717 pages
File Size : 52,9 Mb
Release : 2014-11-10
Category : Science
ISBN : 9783110278965

Get Book

Animal Cell Biotechnology by Hansjörg Hauser,Roland Wagner Pdf

This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Author : Ashish Verma,Anchal Singh
Publisher : Academic Press
Page : 668 pages
File Size : 55,6 Mb
Release : 2013-11-04
Category : Science
ISBN : 9780123914347

Get Book

Animal Biotechnology by Ashish Verma,Anchal Singh Pdf

Animal Biotechnology introduces applications of animal biotechnology and implications for human health and welfare. It begins with an introduction to animal cell cultures and genome sequencing analysis and provides readers with a review of available cell and molecular tools. Topics here include the use of transgenic animal models, tissue engineering, nanobiotechnology, and proteomics. The book then delivers in-depth examples of applications in human health and prospects for the future, including cytogenetics and molecular genetics, xenografts, and treatment of HIV and cancers. All this is complemented by a discussion of the ethical and safety considerations in the field. Animal biotechnology is a broad field encompassing the polarities of fundamental and applied research, including molecular modeling, gene manipulation, development of diagnostics and vaccines, and manipulation of tissue. Given the tools that are currently available and the translational potential for these studies, animal biotechnology has become one of the most essential subjects for those studying life sciences. Highlights the latest biomedical applications of genetically modified and cloned animals with a focus on cancer and infectious diseases Provides firsthand accounts of the use of biotechnology tools, including molecular markers, stem cells, and tissue engineering

Author : Jack G. Chirikjian,Edward Clement Kisailus
Publisher : Jones & Bartlett Learning
Page : 220 pages
File Size : 51,5 Mb
Release : 1995
Category : Animal cell biotechnology
ISBN : 0867208953

Get Book

Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology by Jack G. Chirikjian,Edward Clement Kisailus Pdf

Vol. I: The work presented in these two volumes is the collaborative effort of over twenty undergraduate science faculty, whose common goal was to develop a text of unique and flexible laboratory activities focusing on the theory and practice of biotechnology for undergraduate students. The books are designed to provide flexibility for easy integration into any course in the life sciences with an experimental emphasis.

Author : M. K. Sateesh
Publisher : New Age International
Page : 214 pages
File Size : 42,6 Mb
Release : 2003
Category : Animal cell biotechnology
ISBN : 8122414516

Get Book

Biotechnology-5: Animal Cells, Immunology & Plant Biotechnology by M. K. Sateesh Pdf

This Book Is Designed As Per The Syllabus Of Biotechnology Paper-5 Prescribed By Bangalore University And Other Indian Universities. The Book Is Divided Into Three Parts As Follows: * Animal Cell Biotechnology * Immunology * Plant BiotechnologyThe Presentation In Each Part Is Simple And Systematic. The Basic Concepts Have Been Clearly Explained And Their Functions Are Adequately Highlighted. A Few Recent Developments Have Also Been Included To Provide A Contemporary Understanding Of The Subject.

Author : R. E. Spier,J. B. Griffiths,W. Berthold
Publisher : Elsevier
Page : 852 pages
File Size : 42,9 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483162935

Get Book

Animal Cell Technology by R. E. Spier,J. B. Griffiths,W. Berthold Pdf

Animal Cell Technology: Products of Today, Prospects for Tomorrow is a collection of papers that discusses the advancement and future of biotechnology. The book presents a total of 164 materials that are organized into 22 sections. The coverage of the text includes the various methodologies involved in animal cell technology, such as post translational modifications; kinetics and modeling; and measurement and assay. The book also covers product safety and consistency testing; products from animal cells in culture; and apoptosis and cell biology. The text will be of great use to biologists, biotechnicians, and biological engineers. Readers who have an interest in the advancement of biotechnology will also benefit from the book.

Author : R. E. Spier,J. B. Griffiths,B. Meignier
Publisher : Butterworth-Heinemann
Page : 852 pages
File Size : 50,7 Mb
Release : 2013-09-24
Category : Medical
ISBN : 9781483165158

Get Book

Production of Biologicals from Animal Cells in Culture by R. E. Spier,J. B. Griffiths,B. Meignier Pdf

Production of Biologicals from Animal Cells in Culture reviews the state of the art in animal cell biotechnology, with emphasis on the sequence of events that occur when generating a biological from animal cells in culture. Methods that enable adjustment of nutrient feed streams into perfusion bioreactors so as to increase productivity are described. A number of issues are also addressed, such as the usefulness of the fingerprint method for cell characterization. Comprised of 135 chapters, this book begins with an overview of the problems and benefits of animal cell culture, followed by a discussion on the isolation of immortal murine macrophage cell lines. The reader is systematically introduced to the use of DNA fingerprinting to characterize cell banks; immortalization of cells with oncogenes; lipid metabolism of animal cells in culture; and energetics of glutaminolysis. Subsequent chapters explore serum-free and protein-free media; the physiology of animal cells; gene expression in animal cell systems; and animal cell bioreactors. The monitoring and assay of animal cell parameters are also considered, along with downstream processing and regulatory issues. This monograph will be of interest to students, practitioners, and investigators in the fields of microbiology and biotechnology.

Author : Chester S. Ho
Publisher : Butterworth-Heinemann
Page : 512 pages
File Size : 50,9 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483289472

Get Book

Animal Cell Bioreactors by Chester S. Ho Pdf

Animal Cell Bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. It addresses engineering aspects such as mass transfer, instrumentation, and control ensuring successful design and operation of animal cell bioreactors. The goal is to provide a comprehensive analysis and review in the advancement of the bioreactor systems for large-scale animal cell cultures. The book is organized into four parts. Part I traces the historical development of animal cell biotechnology. It presents examples of work in progress that seeks to make animal cell biotechnology processes as productive on a cost per unit of product basis as that achieved by other microbial systems. Part II includes chapters dealing with the implications of cell biology in animal cell biotechnology; protein-bound oligosaccharides and their structures; the development of serum-free media and its use in the production of biologically active substances; and the metabolism of mammalian cells. Part III focuses on animal cell cultivation, covering topics such as the fixed bed immobilized culture; three-dimensional microcarriers; and hydrodynamic phenomena in microcarrier cultures. Part IV discusses the design, operation, and control of animal cell bioreactors.

Author : National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Board on Agriculture and Natural Resources,Committee on Agricultural Biotechnology, Health, and the Environment,Committee on Defining Science-Based Concerns Associated with Products of Animal Biotechnology
Publisher : National Academies Press
Page : 201 pages
File Size : 40,9 Mb
Release : 2002-12-29
Category : Technology & Engineering
ISBN : 9780309084390

Get Book

Animal Biotechnology by National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Board on Agriculture and Natural Resources,Committee on Agricultural Biotechnology, Health, and the Environment,Committee on Defining Science-Based Concerns Associated with Products of Animal Biotechnology Pdf

Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.

Author : Mell Carter &
Publisher : Scientific e-Resources
Page : 316 pages
File Size : 45,9 Mb
Release : 2019-10-15
Category : Electronic
ISBN : 9781839471575

Get Book

Animal Cell Culture by Mell Carter & Pdf

Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favourable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established. Stem cells retain the capacity to self renew as well as to produce progeny with a restricted mitotic potential and restricted range of distinct types of differentiated cell they give rise to. The formation of blood cells, also called haematopoiesis, is the classical example of concept of stem cells. Animal cell and tissue culture is an integral part of biotechnology and this book covers all the aspects of animal cell culture. Animal cells are used for making new vaccines, specific animal proteins such as intergerons, blood factors and hormones, monoclonal antibodies for use as diagnostic and therapeutics, gene probes as diagnostic too, enzymes and last but not the least many new and important compounds. This book contains eleven Chapters, which deal with historic developments, laboratory design, sterilization procedures and various facets of animal cell culture. This includes preservation, characterizations, storage and transport of cells, their monitoring and technologies for cell banking.

Author : Nigel Jenkins
Publisher : Unknown
Page : 128 pages
File Size : 55,8 Mb
Release : 1999
Category : Electronic
ISBN : OCLC:748540439

Get Book

Animal Cell Biotechnology by Nigel Jenkins Pdf

Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

Author : Michael Butler
Publisher : Taylor & Francis
Page : 299 pages
File Size : 52,8 Mb
Release : 2004-08-02
Category : Medical
ISBN : 9781135325381

Get Book

Animal Cell Culture and Technology by Michael Butler Pdf

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Author : Raymond Spier
Publisher : Unknown
Page : 128 pages
File Size : 51,5 Mb
Release : 1990
Category : Electronic
ISBN : OCLC:632839584

Get Book

Animal cell biotechnology by Raymond Spier Pdf