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Author : Anonim
Publisher : Universities Press
Page : 248 pages
File Size : 53,5 Mb
Release : 2024-06-26
Category : Electronic
ISBN : 9788173717192
Author : Shalini Mani,Manisha Singh,Anil Kumar
Publisher : Springer Nature
Page : 332 pages
File Size : 52,9 Mb
Release : 2023-01-31
Category : Science
ISBN : 9783031194856
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
Author : R. Ian Freshney,Amanda Capes-Davis
Publisher : John Wiley & Sons
Page : 832 pages
File Size : 43,6 Mb
Release : 2021-02-17
Category : Science
ISBN : 9781119513032
FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.
Author : Sinha Basant K.,Kumar Rinesh
Publisher : IBDC Publishers
Page : 310 pages
File Size : 48,7 Mb
Release : 2008
Category : Electronic
ISBN : 8181892488
Author : A Wilson Aruni,P Ramadass
Publisher : MJP Publisher
Page : 219 pages
File Size : 46,7 Mb
Release : 2019-06-10
Category : Fiction
ISBN : 8210379456XXX
Cell and tissues culture has been one of the first and foremost techniques paving for recent cutting-edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. It has played a substantial role in the developments of health care and prophylactics industries, thus serving the mankind. It has made the dream of producing cost-effective prophylactics, diagnostics and therapeutics come true and affordable. In the recent past, with the explosion of knowledge in the field of biotechnology, intensive research in being carried out, where undergraduate and post-graduate courses are being offered in this field. Even through more emphasis is being given to theory, a dearth of practical knowledge is lacking due to paucity of established tissue culture facilities.
Author : John M. Davis
Publisher : John Wiley & Sons
Page : 389 pages
File Size : 45,7 Mb
Release : 2011-03-16
Category : Science
ISBN : 9780470975633
This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
Author : R. Ian Freshney
Publisher : Wiley-Liss
Page : 696 pages
File Size : 44,9 Mb
Release : 2005-07-29
Category : Science
ISBN : 0471453293
The most complete resource on the techniques, equipment,principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: * An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols * New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering * An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment * Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text * Fully updated references * The clearest, most consistent presentation of step-by-step protocols available * Numerous diagrams, photographs, tables, and charts * Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Author : R. Ian Freshney
Publisher : John Wiley & Sons
Page : 766 pages
File Size : 54,5 Mb
Release : 2011-02-02
Category : Science
ISBN : 9780470649350
This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture
Author : Anonim
Publisher : Academic Press
Page : 368 pages
File Size : 50,6 Mb
Release : 1998-07-03
Category : Science
ISBN : 0080859550
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features * Reviews basic principles of cell culture * Gives options and techniques on how to look at cells
Author : Martin Clynes
Publisher : Springer Science & Business Media
Page : 624 pages
File Size : 51,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642804120
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Author : R. Ian Freshney
Publisher : Wiley-Liss
Page : 608 pages
File Size : 46,8 Mb
Release : 2000-07-13
Category : Science
ISBN : 0471388769
The fourth edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting. The advantages of tissue culture go beyond control of the physiochemical environment and physiological conditions as shown in the comprehensive coverage of tissue culture topics, both organ culture and cell culture, provided in this manual. A wide range of essential information from basic to specialized procedures is presented, highlighting advantages and limitations, and illustrating the properties of different types of culture. This crucial reference for cell culture techniques includes: New Atlas of Cells section in full-color presentation Extended coverage of molecular techniques, scale-up, and serum-free medium New chapter on problem solving Photographs of cell lines, contaminations, and equipment Clear and concise tables and charts Educated recommendations on safety issues, ethical consent, and ownership Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference.
Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 766 pages
File Size : 44,9 Mb
Release : 2014-11-28
Category : Medical
ISBN : 9783319103204
Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.
Author : Michael Butler
Publisher : Taylor & Francis
Page : 299 pages
File Size : 49,5 Mb
Release : 2004-08-02
Category : Medical
ISBN : 9781135325381
Provides all essential practical information for establishing a laboratory animal cell culture. Comprehensive glossary of terms.
Author : John Masters
Publisher : OUP Oxford
Page : 334 pages
File Size : 44,9 Mb
Release : 2000-06-29
Category : Science
ISBN : 9780191566042
This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practising cell culture will benefit greatly from this book.
Author : Mather, Jennie P.
Publisher : Unknown
Page : 368 pages
File Size : 51,9 Mb
Release : 2006
Category : Tissue culture
ISBN : OCLC:808866854
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features* Reviews basic principles of cell culture* Gives options and techniques on how to look at cells.
