Antibody Engineering Volume 2

Author : Roland E. Kontermann,Stefan Dübel
Publisher : Springer Science & Business Media
Page : 578 pages
File Size : 45,5 Mb
Release : 2010-03-10
Category : Medical
ISBN : 9783642011474

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Antibody Engineering Volume 2 by Roland E. Kontermann,Stefan Dübel Pdf

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Author : Anonim
Publisher : Springer
Page : 604 pages
File Size : 50,8 Mb
Release : 2011-07-14
Category : Electronic
ISBN : 3642011489

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Antibody Engineering by Anonim Pdf

Author : Roland E. Kontermann,Stefan Dübel
Publisher : Springer Science & Business Media
Page : 778 pages
File Size : 40,7 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783662046050

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Antibody Engineering by Roland E. Kontermann,Stefan Dübel Pdf

Interest in recombinant antibody technologies has rapidly increased because of its wide range of possible applications in therapy, diagnosis, and especially, cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has facilitated the development of antibody engineering technologies. This manual presents a comprehensive collection of detailed step-by-step protocols, provided by experts. The text covers all basic methods needed in antibody engineering as well as recently developed and emerging technologies.

Author : Roland E. Kontermann,Stefan Dübel
Publisher : Springer
Page : 800 pages
File Size : 47,7 Mb
Release : 2010-03-26
Category : Medical
ISBN : 3662495945

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Antibody Engineering by Roland E. Kontermann,Stefan Dübel Pdf

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Author : Roland E. Kontermann,Stefan Dübel
Publisher : Springer Science & Business Media
Page : 788 pages
File Size : 44,9 Mb
Release : 2010-03-10
Category : Medical
ISBN : 9783642011443

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Antibody Engineering Volume 1 by Roland E. Kontermann,Stefan Dübel Pdf

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Author : G. Subramanian
Publisher : Springer Science & Business Media
Page : 252 pages
File Size : 52,9 Mb
Release : 2004-03-31
Category : Medical
ISBN : 0306483157

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Antibodies by G. Subramanian Pdf

It is now over one hundred years since von Behring and Kitsato first concluded experiments that led to the use of passive immunisation, employing antibodies raised in animals against tetanus and diphtheria toxins. The advancement of technology both in manufacturing purity product in a cost effective way and the clinical research has proved that antibodies are one ofthe most successful products in biotechnology. Monoclonal antibodies account for between one-third and one-halfof all pharmaceutical products in development and human clinical trials. Both the nature of monoclonal antibody therapies and the relatively large size of the monoclonal antibody dictate the production requirements, for many ofthese therapeutics the monoclonal antibody product will be 100 kilogrammes or more per year. It is widely acknowledged that there is currently a worldwide shortage of biomanufacturing capacity, and the active pharmaceutical ingredient material requirements for these products are expected to increase. Thus the industry is looking for new sources and extensive studies are being carried out not only for alternative technology to meet the needs but also to reveal the new therapeutic applicationsofantibodies. This book brings to the forefront current advances in novel technologies for the manufacturing of monoclonal antibodies and also their extensive clinical importance. The first four chapters give an overview of the new technologies and the successful application in the manufacture of monoclonal antibodies with clinical purity. The next chapters address the application of antibodies in cancer therapy and functional genomic therapy.

Author : William R Strohl,Lila M Strohl
Publisher : Elsevier
Page : 696 pages
File Size : 40,7 Mb
Release : 2012-10-16
Category : Science
ISBN : 9781908818096

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Therapeutic Antibody Engineering by William R Strohl,Lila M Strohl Pdf

The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering. Goes beyond the standard engineering issues covered by most books and delves into structure-function relationships Integration of knowledge across all areas of antibody engineering, development, and marketing Discusses how current and future genetic engineering of cell lines will pave the way for much higher productivity

Author : Damien Nevoltris,Patrick Chames
Publisher : Humana Press
Page : 543 pages
File Size : 46,7 Mb
Release : 2018-09-24
Category : Medical
ISBN : 1493986473

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Antibody Engineering by Damien Nevoltris,Patrick Chames Pdf

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Author : Florian Rüker,Gordana Wozniak-Knopp
Publisher : Springer Nature
Page : 388 pages
File Size : 53,6 Mb
Release : 2021-01-04
Category : Medical
ISBN : 9783030546304

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Introduction to Antibody Engineering by Florian Rüker,Gordana Wozniak-Knopp Pdf

This highly readable textbook serves as a concise and engaging primer to the emerging field of antibody engineering and its various applications. It introduces readers to the basic science and molecular structure of antibodies, and explores how to characterize and engineer them. Readers will find an overview of the latest methods in antibody identification, improvement and biochemical engineering. Furthermore, alternative antibody formats and bispecific antibodies are discussed. The book’s content is based on lectures for the specializations “Protein Engineering” and “Medical Biotechnology” within the Master’s curriculum in “Biotechnology.” The lectures have been held at the University of Natural Resources and Life Sciences, Vienna, in cooperation with the Medical University of Vienna, since 2012 and are continuously adapted to reflect the latest developments in the field. The book addresses Master’s and PhD students in biotechnology, molecular biology and immunology, and all those who are interested in antibody engineering.

Author : Benny K. C. Lo
Publisher : Springer Science & Business Media
Page : 555 pages
File Size : 52,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596669

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Antibody Engineering by Benny K. C. Lo Pdf

The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Köhler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious—high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host’s immune s- tem for a therapeutic response. The initial excitement in finding Paul Ehrlich’s “magic bullet,” however, was met with widespread disappointment when it was demonstrated that murine antibodies frequently elicit the human anti-murine an- body (HAMA) response, thus rendering them ineffective and potentially unsafe in humans. Despite this setback, advances in recombinant DNA techniques over the last 15–20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA. The ability to p- duce bulk quantities of recombinant proteins from bacterial fermentation also fueled the design of numerous creative antibody constructs. To date, the United States Food and Drug Administration has approved more than 10 recombinant antibodies for human use, and hundreds more are in the development pipeline. The recent explosion in genomic and proteomic information appears ready to deliver many more disease targets amenable to antibody-based therapy.

Author : Carl A. K. Borrebaeck
Publisher : Unknown
Page : 180 pages
File Size : 44,7 Mb
Release : 1992
Category : Immunoglobulins
ISBN : 1561590088

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Antibody Engineering by Carl A. K. Borrebaeck Pdf

Author : Roland E. Kontermann
Publisher : Springer Science & Business Media
Page : 373 pages
File Size : 52,7 Mb
Release : 2011-07-21
Category : Medical
ISBN : 3642209106

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Bispecific Antibodies by Roland E. Kontermann Pdf

The concept of using bispecific antibodies for cancer therapy by retargeting immune effector cells was developed several years ago. Initial clinical studies were rather disappointing mainly due to low efficacy, severe side effects and the immunogenicity of the bispecific antibodies. The progress in antibody engineering finally led to the generation of new classes of bispecific antibodies lacking these obstacles. In addition, new applications were established, such as pre-targeting strategies in radioimmunotherapy and dual targeting approaches in order to improve binding, selectivity and efficacy. In this book, the different ways of generating bispecific antibodies are described, with emphasis on recombinant formats. The various applications of bispecific antibodies, e.g. in cellular cancer immunotherapy, radioimmunotherapy and pretargeting strategies are covered, and emerging applications such as dual targeting strategies, which involve the simultaneous inhibition of two targets, are addressed.

Author : Damien Nevoltris,Patrick Chames
Publisher : Humana
Page : 543 pages
File Size : 55,8 Mb
Release : 2019-12-10
Category : Medical
ISBN : 1493993534

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Antibody Engineering by Damien Nevoltris,Patrick Chames Pdf

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Author : Stefan Dübel,Janice M. Reichert
Publisher : John Wiley & Sons
Page : 2538 pages
File Size : 53,5 Mb
Release : 2014-12-03
Category : Science
ISBN : 9783527329373

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Handbook of Therapeutic Antibodies by Stefan Dübel,Janice M. Reichert Pdf

Still the most comprehensive reference source on the development, production and therapeutic application of antibodies, this second edition is thoroughly updated and now has 30% more content. Volume 1 covers selection and engineering strategies for new antibodies, while the second volume presents novel therapeutic concepts and antibodies in clinical study, as well as their potential. Volumes 3 and 4 feature detailed and specific information about each antibody approved for therapeutic purposes, including clinical data. This unique handbook concludes with a compendium of marketed monoclonal antibodies and an extensive index. Beyond providing current knowledge, the authors discuss emerging technologies, future developments, and intellectual property issues, such that this handbook meets the needs of academic researchers, decision makers in industry and healthcare professionals in the clinic.

Author : G. Subramanian
Publisher : Springer
Page : 550 pages
File Size : 50,8 Mb
Release : 2004-01-31
Category : Medical
ISBN : 0306483343

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Antibodies by G. Subramanian Pdf

If the antibody industry is to achieve its full potential in the next decade, the individual technical potentials must be exploited, the limitations must be addressed, and lessons learned must be applied both to current purification methods and to the new technologies that continue to emerge. This book presents an overview of the current advances applied in the manufacture of monoclonal antibody including: -concepts in development of manufacturing strategies, -importance of antibody fragments, -application of chromatography method development, -quality control, -effect of expression on antibody properties, -virus removal and safety, -pharmacokinetics, -regulatory aspects.