Antisense Technology

Author : Stanley T. Crooke
Publisher : CRC Press
Page : 2004 pages
File Size : 55,6 Mb
Release : 2001-09-14
Category : Medical
ISBN : 0824705661

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Antisense Drug Technology by Stanley T. Crooke Pdf

This state-of-the-art reference provides comprehensive coverage of the development of antisense oligonucleotides to inhibit cancer cells as well as those involved in infectious, inflammatory, and immune-mediated diseases-highlighting new tools and technologies in medicinal chemistry, RNA biochemistry, and molecular and cellular biology to produce new therapeutic compounds. Presents previously unpublished data on the use of antisense technology to dissect pharmacological processes and confirm the roles of various genes! Showcasing the benefits of antisense drug use, including reduced toxicity and earlier disease detection, Antisense Drug Technology discusses novel formulations of antisense drugs practical methods to design effective isotype selective inhibitors molecular mechanisms of antisense drugs mRNA as a current biological template modern postreceptor binding mechanisms and more! With contributions by over 60 seasoned experts in the field and containing more than 3000 helpful references, tables, drawings, and photographs, Antisense Drug Technology is an illuminating source for organic, medicinal, and pharmaceutical chemists; biochemists; geneticists; hematologists; oncologists; molecular and cell biologists; virologists; immunologists; and medical school and graduate students in these disciplines.

Author : Conrad Lichtenstein,Wolfgang Nellen (ed)
Publisher : IRL Press
Page : 334 pages
File Size : 41,8 Mb
Release : 1997
Category : Antisense DNA.
ISBN : 0199635838

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Antisense Technology by Conrad Lichtenstein,Wolfgang Nellen (ed) Pdf

Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologies are then described, with chapters on antisense techniques in IDictyostelium, plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo. Finally, non-antisense effects of oligonucleotides, "anti-sense rescue", shotgun antisense mutagenesis, and in vivo, in vitro and in situ detection of sense: antisense duplexes by structure-specific anti-RNA antibodies are discussed. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, or protein function.

Author : Stanley T. Crooke
Publisher : CRC Press
Page : 848 pages
File Size : 49,7 Mb
Release : 2007-07-25
Category : Medical
ISBN : 9781439832509

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Antisense Drug Technology by Stanley T. Crooke Pdf

Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research. Interpreting lessons learned from the clinical trials of first generati

Author : Anonim
Publisher : Elsevier
Page : 614 pages
File Size : 42,6 Mb
Release : 1999-11-01
Category : Science
ISBN : 9780080496702

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Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies by Anonim Pdf

Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Author : Stanley T. Crooke
Publisher : Springer Science & Business Media
Page : 646 pages
File Size : 54,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642587856

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Antisense Research and Application by Stanley T. Crooke Pdf

Antisense technology may result in dramatic changes in the therapy of many diseases and may provide tools to dissect pharmacological processes and to confirm the roles of various genes. In this volume, progress in the understanding of antisense technology and its use in creating new drugs is discussed. Potential caveats, pitfalls and limitations of the technology are also presented. In the next few years the pace at which new molecular targets will be identified will increase exponentially as the sequencing of the human genome and of other genomes proceeds.

Author : Anonim
Publisher : Elsevier
Page : 683 pages
File Size : 54,6 Mb
Release : 1999-11-10
Category : Science
ISBN : 9780080496719

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Antisense Technology, Part B: Applications by Anonim Pdf

Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedically-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.

Author : Ronald A. Leslie,Jackie Hunter,Harry A. Robertson,Harold A. Robertson
Publisher : Oxford University Press, USA
Page : 280 pages
File Size : 50,5 Mb
Release : 1999
Category : Antisense nucleic acids
ISBN : UOM:39015050759342

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Antisense Technology in the Central Nervous System by Ronald A. Leslie,Jackie Hunter,Harry A. Robertson,Harold A. Robertson Pdf

Antisense technology has been used for several years to "knock down" the expression of specific genes in living tissues to study the functions of their protein products. Specifically designed antisense oligonucleotides (ODNs) are short lengths of man-made genetic material designed to interferewith the production of the protein encoded by the gene. This can shed light on the function of the protein; alternatively, if a disease process results from an excess of a certain protein, the method can in theory correct the problem. Faster than transgenic techniques, but more problematic inpractice, antisense has been seen as something of a "black art". There are many technical problems to understand and overcome.This is the first book to provide a critical review of the uses of this technology in the central nervous system. It includes: Background to the technology problems of design and synthesis of antisense molecules administration in the brain protection of ODNs from metabolic destruction potent forCNS research and therapy. Antisense Technology in the Central Nervous System describes the promises and the pitfalls of this approach, and compares it to other approaches. The practical experience of leading researchers is combined with a thorough description of the theoretical foundation of thetechnique, making the book ideal for researchers and clinicians alike.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 40,7 Mb
Release : 1993
Category : Electronic
ISBN : OCLC:64150672

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Antisense technology by Anonim Pdf

Author : Stanley T. Crooke,Bernard Lebleu
Publisher : CRC Press
Page : 620 pages
File Size : 45,7 Mb
Release : 1993-05-27
Category : Medical
ISBN : 084934705X

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Antisense Research and Applications by Stanley T. Crooke,Bernard Lebleu Pdf

Antisense Research and Applications is a comprehensive review of oligonucleotide research covering molecular biological advances in this field, the current status of antisense drug research, and strategies for future research and therapeutic applications. In bringing together the latest research from an array of authoritative scientists, Antisense Research and Applications provides an integrated conceptual basis for considering oligonucleotide therapeutics. Topics covered in the 32 chapters of this book include nucleic acid structure and function, antisense RNA, medicinal chemistry of oligonucleotides, analogs, pharmacokinetics and toxicology, and activities of current antisense drugs. This volume addresses advances in a broad range of disciplines and is an excellent resource for basic researchers and applied investigators in pharmaceutical laboratories and in such fields as biochemistry and molecular biology.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 49,9 Mb
Release : 2000
Category : Electronic
ISBN : OCLC:760565886

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Antisense Technology Part A by Anonim Pdf

Author : Macy Rose
Publisher : Callisto Reference
Page : 0 pages
File Size : 45,6 Mb
Release : 2023-09-26
Category : Science
ISBN : 1641167858

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Antisense Technology: Methods of Delivery and RNA Studies by Macy Rose Pdf

Antisense RNA (asRNA) refers to a type of single-stranded RNA. It hybridizes with protein coding messenger RNA (mRNA) and complements it to inhibit its translation into a protein. Eukaryotes and prokaryotes, both have naturally occuring asRNAs in them. They can be divided into two types including short and long non-coding RNAs (ncRNAs). asRNAs primarily control the gene expression and are widely utilized as tools for gene knockdown. Antisense technology offers effective experimental methods for examining gene function and gene regulation by modifying gene expression in mammalian cells. Many RNA-based antisense therapies are being developed currently. However, effective delivery of these therapies to target tissues remains the primary barrier in their clinical application. Antisense technology has a wide range of applications in the treatment of numerous diseases such as viral infections, cancer, and immunological, cardiovascular, ocular and neurological diseases. This book contains some path-breaking studies in antisense technology. It consists of contributions made by international experts. This book is a vital tool for all researching or studying antisense RNA as it gives incredible insights into its design and delivery.

Author : Volker A. Erdmann,Jan Barciszewski
Publisher : Springer Science & Business Media
Page : 460 pages
File Size : 40,5 Mb
Release : 2010-09-02
Category : Medical
ISBN : 9783642121685

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RNA Technologies and Their Applications by Volker A. Erdmann,Jan Barciszewski Pdf

RNA technologies are the driving forces of modern medicine and biotechnology. They combine the fields of biochemistry, chemistry, molecular biology, cell biology, physics, nanotechnology and bioinformatics. The combination of these topics is set to revolutionize the medicine of tomorrow. After more than 15 years of extensive research in the field of RNA technologies, the first therapeutics are ready to reach the first patients. Thus we are witnessing the birth of a very exciting time in the development of molecular medicine, which will be based on the methods of RNA technologies. This volume is the first of a series. It covers various aspects of RNA interference and microRNAs, although antisense RNA applications, hammerhead ribozyme structure and function as well as non-coding RNAs are also discussed. The authors are internationally highly respected experts in the field of RNA technologies.

Author : Cy A. Stein,Arthur M. Krieg
Publisher : Wiley-Liss
Page : 556 pages
File Size : 41,8 Mb
Release : 1998-03-19
Category : Medical
ISBN : UOM:39015047084978

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Applied Antisense Oligonucleotide Technology by Cy A. Stein,Arthur M. Krieg Pdf

Applied Antisense Oligonucleotide Technology provides the basic concepts as well as the practical concerns associated with the use of antisense oligonucleotides to modify gene expression. Chapters include oligonucleotide chemistry, DNA triplex formation, delivery mechanisms, pharmocokinetics, toxicity, oligonucleotides.

Author : Virginia Arechavala-Gomeza,Alejandro Garanto
Publisher : Humana
Page : 422 pages
File Size : 55,8 Mb
Release : 2022-02-26
Category : Psychology
ISBN : 1071620096

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Antisense RNA Design, Delivery, and Analysis by Virginia Arechavala-Gomeza,Alejandro Garanto Pdf

This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antisense RNA Design, Delivery, and Analysis provides basic knowledge and a large collection of methods to facilitate the work of newcomers to this vibrant and expanding field. This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics). DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to enhance interaction and collaborations between researchers in Europe and other countries.

Author : Margaret M. McCarthy
Publisher : Springer Science & Business Media
Page : 179 pages
File Size : 44,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461549338

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Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning by Margaret M. McCarthy Pdf

When first introduced, antisense oligonucleotides were hailed as the long awaited magic bullet that would provide an unprecedented level of specificity in controlling gene expression. Following this initial enthusiasm, antisense oligonucleotides have been maligned as nonspecific, toxic, and essentially useless. However, application of antisense oligonucleotide technology in the nervous system stands apart from the use of this technique in peripheral systems, largely because of its enormous success. The source of this success remains a matter of some controversy. Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning addresses the origins of that controversy and determines whether the nervous system is a privileged site for antisense oligonucleotide action and not subject to the same vagaries and pitfalls as non-neuronal systems. Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning contains chapters by experts in the field that focus on the use of this technique in a variety of behavioral systems, as well as rapid and nonspecific effects and the uptake and metabolism of antisense oligonucleotides by the nervous system. Modulating Gene Expression by Antisense Oligonucleotides to Understand Neural Functioning features: experts in the field reporting on the use of antisense oligonucleotide technology in a variety of behavioral systems, including pain control, circadian rhythms, ingestion and control of water balance, and reproductive behaviors; the novel use of plasmids to express antisense RNA in the nervous system; the biodistribution and metabolism of antisense oligonucleotides in the nervous system; rapid and unusual effects; and non-specific effects.