Rna Technologies And Their Applications

Rna Technologies And Their Applications Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Rna Technologies And Their Applications book. This book definitely worth reading, it is an incredibly well-written.

RNA Technologies and Their Applications

Volker A. Erdmann,Jan Barciszewski
RNA Technologies and Their Applications Book PDF

Author : Volker A. Erdmann,Jan Barciszewski
Publisher : Springer Science & Business Media
Page : 460 pages
File Size : 45,5 Mb
Release : 2010-09-02
Category : Medical
ISBN : 9783642121685

Get Book

RNA Technologies and Their Applications by Volker A. Erdmann,Jan Barciszewski Pdf

RNA technologies are the driving forces of modern medicine and biotechnology. They combine the fields of biochemistry, chemistry, molecular biology, cell biology, physics, nanotechnology and bioinformatics. The combination of these topics is set to revolutionize the medicine of tomorrow. After more than 15 years of extensive research in the field of RNA technologies, the first therapeutics are ready to reach the first patients. Thus we are witnessing the birth of a very exciting time in the development of molecular medicine, which will be based on the methods of RNA technologies. This volume is the first of a series. It covers various aspects of RNA interference and microRNAs, although antisense RNA applications, hammerhead ribozyme structure and function as well as non-coding RNAs are also discussed. The authors are internationally highly respected experts in the field of RNA technologies.

Gene Silencing by RNA Interference

Muhammad Sohail
Gene Silencing by RNA Interference Book PDF

Author : Muhammad Sohail
Publisher : CRC Press
Page : 400 pages
File Size : 52,7 Mb
Release : 2004-08-27
Category : Science
ISBN : 9780203489253

Get Book

Gene Silencing by RNA Interference by Muhammad Sohail Pdf

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.

Gene Silencing by RNA Interference

Muhammad Sohail
Gene Silencing by RNA Interference Book PDF

Author : Muhammad Sohail
Publisher : CRC Press
Page : 400 pages
File Size : 53,5 Mb
Release : 2004-08-27
Category : Science
ISBN : 0849321417

Get Book

Gene Silencing by RNA Interference by Muhammad Sohail Pdf

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.

Gene Expression and Regulation in Mammalian Cells

Fumiaki Uchiumi
Gene Expression and Regulation in Mammalian Cells Book PDF

Author : Fumiaki Uchiumi
Publisher : BoD – Books on Demand
Page : 374 pages
File Size : 45,5 Mb
Release : 2018-02-28
Category : Medical
ISBN : 9789535138679

Get Book

Gene Expression and Regulation in Mammalian Cells by Fumiaki Uchiumi Pdf

Sixty years after the "central dogma," great achievements have been developed in molecular biology. We have also learned the important functions of noncoding RNAs and epigenetic regulations. More importantly, whole genome sequencing and transcriptome analyses enabled us to diagnose specific diseases. This book is not only intended for students and researchers working in laboratory but also physicians and pharmacists. This volume consists of 14 chapters, divided into 4 parts. Each chapter is written by experts investigating biological stresses, epigenetic regulation, and functions of transcription factors in human diseases. All articles presented in this volume by excellent investigators provide new insights into the studies in transcriptional control in mammalian cells and will inspire us to develop or establish novel therapeutics against human diseases.

RNA-Based Technologies for Functional Genomics in Plants

Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh
RNA Based Technologies for Functional Genomics in Plants Book PDF

Author : Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh
Publisher : Springer Nature
Page : 428 pages
File Size : 52,8 Mb
Release : 2021-05-13
Category : Science
ISBN : 9783030649944

Get Book

RNA-Based Technologies for Functional Genomics in Plants by Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh Pdf

This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for genes. Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further development and applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.

RNA Interference and CRISPR Technologies

Mouldy Sioud
RNA Interference and CRISPR Technologies Book PDF

Author : Mouldy Sioud
Publisher : Unknown
Page : 486 pages
File Size : 55,6 Mb
Release : 2020
Category : Gene expression
ISBN : 107160290X

Get Book

RNA Interference and CRISPR Technologies by Mouldy Sioud Pdf

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. .

RNAi Technology

R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
RNAi Technology Book PDF

Author : R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
Publisher : CRC Press
Page : 380 pages
File Size : 48,9 Mb
Release : 2016-04-19
Category : Science
ISBN : 9781439873250

Get Book

RNAi Technology by R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta Pdf

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

RNA Interference Technology

Krishnarao Appasani
RNA Interference Technology Book PDF

Author : Krishnarao Appasani
Publisher : Cambridge University Press
Page : 544 pages
File Size : 47,8 Mb
Release : 2005-01-17
Category : Science
ISBN : 0521836778

Get Book

RNA Interference Technology by Krishnarao Appasani Pdf

RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this 2005 overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.

Epitranscriptomics

Stefan Jurga,Jan Barciszewski
Epitranscriptomics Book PDF

Author : Stefan Jurga,Jan Barciszewski
Publisher : Springer Nature
Page : 632 pages
File Size : 51,5 Mb
Release : 2021-07-28
Category : Science
ISBN : 9783030716127

Get Book

Epitranscriptomics by Stefan Jurga,Jan Barciszewski Pdf

This book reviews a novel and exciting field of cellular and molecular biology called epitranscriptomics, which focuses on changes in an organism’s cells resulting from the posttranscriptional modification of cellular RNA. RNA-binding proteins (RBPs) play a crucial role in these posttranscriptional modifications and also support several cellular processes necessary for maintaining RNA homeostasis. Exploring the mechanisms underlying RNA modifications and RBP function is an emerging area of biomedical research, taking the study of gene regulation a step beyond epigenetics. This book reveals that the RNA molecule is not just an information-carrying molecule with some secondary structures. Accordingly, how RNA is modified, regulated, packaged, and controlled is an important aspect. Leading experts address questions such as where the over 170 distinct posttranscriptional RNA modifications are located on the genome, what percentage of mRNAs and noncoding RNAs these modifications include, and how an RNA modification impacts a person’s biology. In closing, the book reviews the role of RNA modifications and RBPs in a variety of diseases and their pathogenesis. Addressing some of the most exciting challenges in epitranscriptomics, this book provides a valuable and engaging resource for researchers in academia and industry studying the phenomena of RNA modification.

Evolution of Translational Omics

Institute of Medicine,Board on Health Sciences Policy,Board on Health Care Services,Committee on the Review of Omics-Based Tests for Predicting Patient Outcomes in Clinical Trials
Evolution of Translational Omics Book PDF

Author : Institute of Medicine,Board on Health Sciences Policy,Board on Health Care Services,Committee on the Review of Omics-Based Tests for Predicting Patient Outcomes in Clinical Trials
Publisher : National Academies Press
Page : 354 pages
File Size : 43,5 Mb
Release : 2012-09-13
Category : Science
ISBN : 9780309224185

Get Book

Evolution of Translational Omics by Institute of Medicine,Board on Health Sciences Policy,Board on Health Care Services,Committee on the Review of Omics-Based Tests for Predicting Patient Outcomes in Clinical Trials Pdf

Technologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials.

RNA Therapeutics

Mouldy Sioud
RNA Therapeutics Book PDF

Author : Mouldy Sioud
Publisher : Humana Press
Page : 527 pages
File Size : 47,7 Mb
Release : 2011-03-04
Category : Science
ISBN : 1607616645

Get Book

RNA Therapeutics by Mouldy Sioud Pdf

Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular BiologyTM series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes. Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.

Plant Small RNA

Praveen Guleria,Vineet Kumar
Plant Small RNA Book PDF

Author : Praveen Guleria,Vineet Kumar
Publisher : Academic Press
Page : 638 pages
File Size : 52,7 Mb
Release : 2020-02-19
Category : Science
ISBN : 9780128173367

Get Book

Plant Small RNA by Praveen Guleria,Vineet Kumar Pdf

Plant Small RNA: Biogenesis, Regulation and Application describes the biosynthesis of small RNA in plant systems. With an emphasis on the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival, this books presents the basics and most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. In addition, it emphasizes the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival. Final sections cover the most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. Presents foundational information about small RNA biology and regulation in plants Includes small RNA pathway advances Describes the application and scope of small RNA technology for agricultural stability

MicroRNA and Non-Coding RNA

James C. Johnson
MicroRNA and Non Coding RNA Book PDF

Author : James C. Johnson
Publisher : Nova Biomedical Books
Page : 165 pages
File Size : 49,7 Mb
Release : 2014-05-14
Category : Medical
ISBN : 1626184437

Get Book

MicroRNA and Non-Coding RNA by James C. Johnson Pdf

This book presents current research in the study of new technologies, developments and applications of microRNA and non-coding RNA. Topics include the role of microRNA and short interfering RNAs in plants; microRNAs, major affective disorders and suicidal behavior; microRNA in endoparasites; the functional roles of non-coding RNAs in glioma and their clinical implications; identifying miRNA function in innate immunity; and how multiple IsomiRs and diversity of miRNA sequences unveil evolutionary roles and functional relationships across animals.

RNA Technologies in Cardiovascular Medicine and Research

Volker A. Erdmann,Wolfgang Poller,Jan Barciszewski
RNA Technologies in Cardiovascular Medicine and Research Book PDF

Author : Volker A. Erdmann,Wolfgang Poller,Jan Barciszewski
Publisher : Springer
Page : 358 pages
File Size : 53,9 Mb
Release : 2009-08-29
Category : Science
ISBN : 3540871233

Get Book

RNA Technologies in Cardiovascular Medicine and Research by Volker A. Erdmann,Wolfgang Poller,Jan Barciszewski Pdf

In this book the authors review the field and explore the potential role of RNAi and other RNA technologies in cardiovascular medicine and research. They highlight the impressive recent progress but also the hurdles that still must be overcome before this promising technology is finally ready for translation and clinical use.

Innovative Medicine

Kazuwa Nakao,Nagahiro Minato,Shinji Uemoto
Innovative Medicine Book PDF

Author : Kazuwa Nakao,Nagahiro Minato,Shinji Uemoto
Publisher : Springer
Page : 330 pages
File Size : 54,8 Mb
Release : 2015-10-13
Category : Science
ISBN : 9784431556510

Get Book

Innovative Medicine by Kazuwa Nakao,Nagahiro Minato,Shinji Uemoto Pdf

This book is devoted to innovative medicine, comprising the proceedings of the Uehara Memorial Foundation Symposium 2014. It remains extremely rare for the findings of basic research to be developed into clinical applications, and it takes a long time for the process to be achieved. The task of advancing the development of basic research into clinical reality lies with translational science, yet the field seems to struggle to find a way to move forward. To create innovative medical technology, many steps need to be taken: development and analysis of optimal animal models of human diseases, elucidation of genomic and epidemiological data, and establishment of “proof of concept”. There is also considerable demand for progress in drug research, new surgical procedures, and new clinical devices and equipment. While the original research target may be rare diseases, it is also important to apply those findings more broadly to common diseases. The book covers a wide range of topics and is organized into three complementary parts. The first part is basic research for innovative medicine, the second is translational research for innovative medicine, and the third is new technology for innovative medicine. This book helps to understand innovative medicine and to make progress in its realization.