Applications Of Flow Cytometry In Stem Cell Research And Tissue Regeneration

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Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Awtar Krishan,H. Krishnamurthy,Satish Totey
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration Book PDF

Author : Awtar Krishan,H. Krishnamurthy,Satish Totey
Publisher : John Wiley and Sons
Page : 312 pages
File Size : 49,5 Mb
Release : 2011-05-12
Category : Science
ISBN : 9781118125854

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Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration by Awtar Krishan,H. Krishnamurthy,Satish Totey Pdf

A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.

Stem Cell-based Tissue Repair

Raphael Gorodetsky,Richard Schäfer
Stem Cell based Tissue Repair Book PDF

Author : Raphael Gorodetsky,Richard Schäfer
Publisher : Royal Society of Chemistry
Page : 405 pages
File Size : 47,9 Mb
Release : 2011
Category : Medical
ISBN : 9781849730013

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Stem Cell-based Tissue Repair by Raphael Gorodetsky,Richard Schäfer Pdf

This unique book by leading experts reviews the key therapeutic issues related to stem cell technology.

Advanced Flow Cytometry: Applications in Biological Research

R.C. Sobti,A. Krishan
Advanced Flow Cytometry Applications in Biological Research Book PDF

Author : R.C. Sobti,A. Krishan
Publisher : Springer Science & Business Media
Page : 152 pages
File Size : 55,8 Mb
Release : 2003-07-31
Category : Medical
ISBN : 1402014848

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Advanced Flow Cytometry: Applications in Biological Research by R.C. Sobti,A. Krishan Pdf

Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.

Stem Cells in Clinical Practice and Tissue Engineering

Rakesh Sharma
Stem Cells in Clinical Practice and Tissue Engineering Book PDF

Author : Rakesh Sharma
Publisher : BoD – Books on Demand
Page : 353 pages
File Size : 55,7 Mb
Release : 2018-05-02
Category : Science
ISBN : 9781789230109

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Stem Cells in Clinical Practice and Tissue Engineering by Rakesh Sharma Pdf

Stem Cells in Clinical Practice and Tissue Engineering is a concise book on applied methods of stem cell differentiation and optimization using tissue engineering methods. These methods offer immediate use in clinical regenerative medicine. The present volume will serve the purpose of applied stem cell differentiation optimization methods in clinical research projects, as well as be useful to relatively experienced stem cell scientists and clinicians who might wish to develop their stem cell clinical centers or research labs further. Chapters are arranged in the order of basic concepts of stem cell differentiation, clinical applications of pluripotent stem cells in skin, cardiac, bone, dental, obesity centers, followed by tissue engineering, new materials used, and overall evaluation with their permitted legal status.

Stem Cell Assays

Mohan C. Vemuri
Stem Cell Assays Book PDF

Author : Mohan C. Vemuri
Publisher : Springer Science & Business Media
Page : 815 pages
File Size : 53,9 Mb
Release : 2007-08-10
Category : Medical
ISBN : 9781588297440

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Stem Cell Assays by Mohan C. Vemuri Pdf

The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.

Embryonic Stem Cell Protocols

Kursad Turksen
Embryonic Stem Cell Protocols Book PDF

Author : Kursad Turksen
Publisher : Springer Science & Business Media
Page : 504 pages
File Size : 41,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450379

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Embryonic Stem Cell Protocols by Kursad Turksen Pdf

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Stem Cells in Regenerative Medicine

Julie Audet,William L. Stanford
Stem Cells in Regenerative Medicine Book PDF

Author : Julie Audet,William L. Stanford
Publisher : Methods in Molecular Biology
Page : 472 pages
File Size : 43,8 Mb
Release : 2009
Category : Science
ISBN : UCSD:31822030258412

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Stem Cells in Regenerative Medicine by Julie Audet,William L. Stanford Pdf

Here, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo.

Advances in Application of Stem Cells: From Bench to Clinics

Firdos Alam Khan
Advances in Application of Stem Cells From Bench to Clinics Book PDF

Author : Firdos Alam Khan
Publisher : Unknown
Page : 0 pages
File Size : 46,6 Mb
Release : 2021
Category : Electronic
ISBN : 303078102X

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Advances in Application of Stem Cells: From Bench to Clinics by Firdos Alam Khan Pdf

The field of stem cell biology is expanding with a continued surge of new information related to its applications. Over past few years, stem cells have been extensively used in cell therapy, tissue engineering, in vitro drug testing among others. At the moment there is no single book available which comprehensively describes the significance of various application of stem cells derived from embryonic and adult sources from lab to clinics. In this edited volume, we discuss basics and advanced topics of stem cells to help researchers, students and professional find the most important information in a single source of updated information about stem cells and relevant applications. This book is divided in 12 chapters and covers topics such as in vitro cell culture, 3D cell culture, cell therapy, tissue engineering, cell factory, cell functionality, in vitro drug testing, organ development, autologous transplantation, allogeneic transplantation, adult stem cells, multipotent stem cells, induced pluripotent stem cells, a pluripotent and embryonic stem cells.

Flow Cytometry and Cell Sorting

Andreas Radbruch
Flow Cytometry and Cell Sorting Book PDF

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
Page : 365 pages
File Size : 51,7 Mb
Release : 2013-03-14
Category : Science
ISBN : 9783662041291

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Flow Cytometry and Cell Sorting by Andreas Radbruch Pdf

The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Adipose-Derived Stem Cells

Jeffrey M. Gimble,Bruce A. Bunnell
Adipose Derived Stem Cells Book PDF

Author : Jeffrey M. Gimble,Bruce A. Bunnell
Publisher : Humana Press
Page : 474 pages
File Size : 52,6 Mb
Release : 2011-08-24
Category : Science
ISBN : 1617379611

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Adipose-Derived Stem Cells by Jeffrey M. Gimble,Bruce A. Bunnell Pdf

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

Stem Cells and the Future of Regenerative Medicine

Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research
Stem Cells and the Future of Regenerative Medicine Book PDF

Author : Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research
Publisher : National Academies Press
Page : 112 pages
File Size : 52,7 Mb
Release : 2002-01-25
Category : Science
ISBN : 9780309170420

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Stem Cells and the Future of Regenerative Medicine by Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research Pdf

Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.

Stem Cell Protocols

Ivan N. Rich
Stem Cell Protocols Book PDF

Author : Ivan N. Rich
Publisher : Humana Press
Page : 0 pages
File Size : 45,6 Mb
Release : 2014-11-11
Category : Science
ISBN : 1493917846

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Stem Cell Protocols by Ivan N. Rich Pdf

This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.

Stem Cells

Mariusz Z. Ratajczak
Stem Cells Book PDF

Author : Mariusz Z. Ratajczak
Publisher : Springer Nature
Page : 410 pages
File Size : 55,8 Mb
Release : 2020-01-02
Category : Science
ISBN : 9783030312060

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Stem Cells by Mariusz Z. Ratajczak Pdf

Since different types of stem cells for therapeutic applications have recently been proposed, this timely volume explores various sources of stem cells for tissue and organ regeneration and discusses their advantages and limitations. Also discussed are pros and cons for using embryonic stem cells, induced pluripotent stem cells, and adult stem cells isolated from postnatal tissues. Different types of adult stem cells for therapeutic applications are also reviewed, including hematopoietic stem cells, epidermal stem cells, endothelial progenitors, neural stem cells, mesenchymal stem cells, and very small embryonic-like stem cells. This book also addresses paracrine effects of stem cells in regenerative medicine that are mediated by extracellular microvesicles and soluble secretome. Finally, potential applications of stem cells in cardiology, gastroenterology, neurology, immunotherapy, and aging are presented. This is an ideal book for students and researchers working in the stem cell research field.

Cancer Stem Cells

Stanley Shostak
Cancer Stem Cells Book PDF

Author : Stanley Shostak
Publisher : BoD – Books on Demand
Page : 634 pages
File Size : 46,5 Mb
Release : 2011-08-01
Category : Medical
ISBN : 9789533075808

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Cancer Stem Cells by Stanley Shostak Pdf

Over the last thirty years, the foremost inspiration for research on metastasis, cancer recurrence, and increased resistance to chemo- and radiotherapy has been the notion of cancer stem cells.The twenty-eight chapters assembled in Cancer Stem Cells - The Cutting Edge summarize the work of cancer researchers and oncologists at leading universities and hospitals around the world on every aspect of cancer stem cells, from theory and models to specific applications (glioma), from laboratory research on signal pathways to clinical trials of bio-therapies using a host of devices, from solutions to laboratory problems to speculation on cancers' stem cells' evolution. Cancer stem cells may or may not be a subset of slowly dividing cancer cells that both disseminate cancers and defy oncotoxic drugs and radiation directed at rapidly dividing bulk cancer cells, but research on cancer stem cells has paid dividends for cancer prevention, detection, targeted treatment, and improved prognosis.

The Science of Stem Cells

Jonathan M. W. Slack
The Science of Stem Cells Book PDF

Author : Jonathan M. W. Slack
Publisher : John Wiley & Sons
Page : 290 pages
File Size : 49,7 Mb
Release : 2018-01-16
Category : Science
ISBN : 9781119235156

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The Science of Stem Cells by Jonathan M. W. Slack Pdf

Introduces all of the essential cell biology and developmental biology background for the study of stem cells This book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells—especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions. Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells. Content focused on the basic science underpinning stem cell biology Covers techniques of studying cell properties and cell lineage in vivo and in vitro Explains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division Includes instructor resources such as further reading and figures for downloading Offers an online supplement summarizing current clinical applications of stem cells Written by a prominent leader in the field, The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology.