Advanced Flow Cytometry Applications In Biological Research

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Advanced Flow Cytometry: Applications in Biological Research

R.C. Sobti,A. Krishan
Advanced Flow Cytometry Applications in Biological Research Book PDF

Author : R.C. Sobti,A. Krishan
Publisher : Springer Science & Business Media
Page : 114 pages
File Size : 49,5 Mb
Release : 2013-04-17
Category : Medical
ISBN : 9789401706230

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Advanced Flow Cytometry: Applications in Biological Research by R.C. Sobti,A. Krishan Pdf

Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.

Flow Cytometry

Andrew Yen
Flow Cytometry Book PDF

Author : Andrew Yen
Publisher : CRC Press
Page : 364 pages
File Size : 48,7 Mb
Release : 1989-08-31
Category : Science
ISBN : 084934834X

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Flow Cytometry by Andrew Yen Pdf

The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.

Flow Cytometry Advd Res & Clinical Applns

Andrew Yen
Flow Cytometry Advd Res Clinical Applns Book PDF

Author : Andrew Yen
Publisher : CRC-Press
Page : 360 pages
File Size : 48,5 Mb
Release : 1989-08-31
Category : Science
ISBN : 084934834X

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Flow Cytometry Advd Res & Clinical Applns by Andrew Yen Pdf

The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.

Flow Cytometry

Andrew Yen
Flow Cytometry Book PDF

Author : Andrew Yen
Publisher : CRC-Press
Page : 312 pages
File Size : 49,9 Mb
Release : 1989-08-31
Category : Science
ISBN : 0849348358

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Flow Cytometry by Andrew Yen Pdf

The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.

Flow Cytometry

Marion G. Macey
Flow Cytometry Book PDF

Author : Marion G. Macey
Publisher : Springer Science & Business Media
Page : 294 pages
File Size : 44,5 Mb
Release : 2007-11-03
Category : Medical
ISBN : 9781597454513

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Flow Cytometry by Marion G. Macey Pdf

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Flow Cytometry

Stefanos Papandreou
Flow Cytometry Book PDF

Author : Stefanos Papandreou
Publisher : Nova Science Publishers
Page : 0 pages
File Size : 49,5 Mb
Release : 2013
Category : Flow cytometry
ISBN : 1628087099

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Flow Cytometry by Stefanos Papandreou Pdf

Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characterisation of T regulatory and immune suppressor cells; multicolor panel design strategies in rare event analysis of dendritic cells and regulatory T cells; advanced characterisation of individual particle morphology light-scattering flow cytometry; and application of flow cytometry in environmental science.

Flow Cytometry Protocols

Teresa S. Hawley,Robert Hawley
Flow Cytometry Protocols Book PDF

Author : Teresa S. Hawley,Robert Hawley
Publisher : Springer Science & Business Media
Page : 436 pages
File Size : 55,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597734

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Flow Cytometry Protocols by Teresa S. Hawley,Robert Hawley Pdf

Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).

Advanced Optical Flow Cytometry

Valery V. Tuchin
Advanced Optical Flow Cytometry Book PDF

Author : Valery V. Tuchin
Publisher : John Wiley & Sons
Page : 698 pages
File Size : 54,7 Mb
Release : 2011-03-31
Category : Science
ISBN : 9783527634293

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Advanced Optical Flow Cytometry by Valery V. Tuchin Pdf

A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Awtar Krishan,H. Krishnamurthy,Satish Totey
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration Book PDF

Author : Awtar Krishan,H. Krishnamurthy,Satish Totey
Publisher : John Wiley and Sons
Page : 312 pages
File Size : 42,7 Mb
Release : 2011-05-12
Category : Science
ISBN : 9781118125854

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Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration by Awtar Krishan,H. Krishnamurthy,Satish Totey Pdf

A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.

Flow Cytometry

Zbigniew Darzynkiewicz,John Paul Robinson,Leslie Wilson,Harry A. Crissman,Paul T. Matsudaira
Flow Cytometry Book PDF

Author : Zbigniew Darzynkiewicz,John Paul Robinson,Leslie Wilson,Harry A. Crissman,Paul T. Matsudaira
Publisher : Academic Press
Page : 638 pages
File Size : 49,5 Mb
Release : 1994
Category : Science
ISBN : WISC:89052220563

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Flow Cytometry by Zbigniew Darzynkiewicz,John Paul Robinson,Leslie Wilson,Harry A. Crissman,Paul T. Matsudaira Pdf

Flow cytometry is a universally used method for sorting cells from biological samples. Cells, first given fluorescent tags which can be excited by a laser system, are then processed through a machine called aflow cytometer. The flow cytometer acts to excite, separate, count and collect the tagged cells. Flow cytometry facilities exist in all major biomedical and biological research centers. The techniques described in Flow Cytometry, Second Edition are essential to today's basic and clinical researchers. Rapid progress in this area necessitates updating the first edition with the second. In ad.

Flow Cytometry

M. G. Ormerod
Flow Cytometry Book PDF

Author : M. G. Ormerod
Publisher : Oxford University Press
Page : 300 pages
File Size : 52,6 Mb
Release : 2000-05-18
Category : Medical
ISBN : 0199638241

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Flow Cytometry by M. G. Ormerod Pdf

Flow cytometry is a technique widely used in biological research and in diagnostic medicine. Flow cytometers are found in most biological research institutions and most clinical laboratories in larger hospitals.

Flow Cytometry, Part B

Anonim
Flow Cytometry Part B Book PDF

Author : Anonim
Publisher : Elsevier
Page : 697 pages
File Size : 52,9 Mb
Release : 1994-12-23
Category : Science
ISBN : 9780080859408

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Flow Cytometry, Part B by Anonim Pdf

From the Reviews of the First Edition: "This is a good reference manual for multi-user facility faced with a wide variety of biological applications." - CYTOMETRY "Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology." - KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS Flow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells. * * Comprehensive methodological coverage in unique style * In depth treatment of procedures * Description of each procedure's: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the technique * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry * 10 methods cover assessment of apoptosis and other modes of cell death * Practical, handbook-style presentation works in lab or classroom * Printed on acid-free paper * Color plates

Flow Cytometry for Biotechnology

Larry A. Sklar
Flow Cytometry for Biotechnology Book PDF

Author : Larry A. Sklar
Publisher : Oxford University Press
Page : 401 pages
File Size : 45,9 Mb
Release : 2005-09-02
Category : Science
ISBN : 9780190293031

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Flow Cytometry for Biotechnology by Larry A. Sklar Pdf

Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

Practical Flow Cytometry

Howard M. Shapiro
Practical Flow Cytometry Book PDF

Author : Howard M. Shapiro
Publisher : John Wiley & Sons
Page : 736 pages
File Size : 45,7 Mb
Release : 2005-02-25
Category : Science
ISBN : 9780471434030

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Practical Flow Cytometry by Howard M. Shapiro Pdf

From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Flow Cytometry

Anonim
Flow Cytometry Book PDF

Author : Anonim
Publisher : Elsevier
Page : 591 pages
File Size : 50,8 Mb
Release : 1994-12-12
Category : Science
ISBN : 9780080859392

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Flow Cytometry by Anonim Pdf

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry