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Author : Sidney Fleischer,Becca Fleischer
Publisher : Academic Press
Page : 720 pages
File Size : 44,8 Mb
Release : 1983-08-28
Category : Science
ISBN : WISC:89031249493
Prokaryotic membranes; Mitochondria; Chloroplasts; Summary of membrane proteins.
Author : Anonim
Publisher : Academic Press
Page : 928 pages
File Size : 43,6 Mb
Release : 1982-05-12
Category : Science
ISBN : 0121819817
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Author : Robert M. Burton
Publisher : Springer
Page : 415 pages
File Size : 49,9 Mb
Release : 2012-11-25
Category : Science
ISBN : 146844798X
A NATO Advanced Study Institute held in Portugal in 1968 on cellular biology directed our attention to the important role that biological membranes played in the fundamental life processes, e.g. the transportation of cellular nutrients into cells and into subcellular par ticles coupled with energy use/or generation. A consi deration of biomembranes and their structure led us to organize a self-supporting course in 1972 under the gen eral sponsorship of the American Oil Chemists' Society on "Fundamentals of Lipid Chemistry." This course con cluded with important papers on lipoproteins, membrane structure and membrane models. The papers presented at the short course were published under the same title. With the background of the lipid course, a third program was developed entitled "Biomembranes - Lipids, Proteins and Receptors" and held as a NATO Advanced Study Insti tute in 1974. This ASI explored the structure of bio membranes and the relationship of non-lipid components to membrane function. This ASI was also published to provide the conference proceedings to interested scien tists who could not attend.
Author : Anonim
Publisher : Elsevier
Page : 392 pages
File Size : 50,8 Mb
Release : 2007-11-12
Category : Science
ISBN : 0080554091
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.
Author : Anonim
Publisher : Elsevier
Page : 480 pages
File Size : 55,6 Mb
Release : 2007-09-13
Category : Science
ISBN : 0080550916
The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in regards to the isolation, characterization, and reconstitution of ribonucleoprotein complexes, expanding the experimental repertoire available to all RNA researchers.
Author : C. H.W. Hirs,Serge N. Timasheff
Publisher : Elsevier
Page : 684 pages
File Size : 45,6 Mb
Release : 1986-09-28
Category : Science
ISBN : 0121820319
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Author : Harry Walter,Göte Johansson
Publisher : Elsevier
Page : 768 pages
File Size : 40,6 Mb
Release : 1994-04-18
Category : Nature
ISBN : 0121821293
General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Author : Anonim
Publisher : Elsevier
Page : 861 pages
File Size : 41,7 Mb
Release : 1999-09-22
Category : Science
ISBN : 9780080496672
This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Author : Krzysztof Palczewski
Publisher : Academic Press
Page : 999 pages
File Size : 48,9 Mb
Release : 2000
Category : Cellular signal transduction
ISBN : 9780121822163
Author : Anonim
Publisher : Elsevier
Page : 736 pages
File Size : 53,6 Mb
Release : 2006-11-02
Category : Science
ISBN : 9780080468983
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
Author : Robert Allison Harris,John Robert Sokatch
Publisher : Academic Press
Page : 587 pages
File Size : 55,5 Mb
Release : 2000
Category : Amino Acids
ISBN : 9780121822255
Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.
Author : Ravi Iyengar
Publisher : Elsevier
Page : 492 pages
File Size : 49,9 Mb
Release : 1994-09-08
Category : Medical
ISBN : 0121821390
This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.
Author : Eric F. Johnson,Michael R. Waterman
Publisher : Academic Press
Page : 461 pages
File Size : 47,5 Mb
Release : 2002
Category : Biochemistry
ISBN : 9780121822606
Human genomics and genetics; Structure and mechanism; Regulation of expression; Metabolism; Invertebrate P450s.
Author : John Abelson
Publisher : Elsevier
Page : 546 pages
File Size : 43,9 Mb
Release : 1996
Category : Combinatorial chemistry
ISBN : 0121821684
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Phage display libraries* Repression fusion proteins* Polysome libraries* Peptide libraries* Nucleic acid libraries* Other small molecule libraries.
Author : W. E. Balch,Channing J. Der,Alan Hall
Publisher : Gulf Professional Publishing
Page : 946 pages
File Size : 45,6 Mb
Release : 2005-12-13
Category : Science
ISBN : 0121828085
Rab GTPases now comprise a family of >63 members. They are emerging as the key hub element controlling the membrane architecture of eukaryotic cells. They are intimately involved in vesicle targeting and fusion in both the endocytic and exocytic pathways and direct the assembly and disassembly of protein complexes that include regulators (GEFs and GAPs), effectors (tethers/motors) and fusion components (SNAREs) that control membrane targeting and fusion. During the last 3 years the field has virtually exploded with the identification and characterization of many new Rab proteins and their effectors. Our understanding of how Rab GTPases control membrane function remains at its infancy. This volume of Methods in Enzymology, GTPases Regulating Membrane Targeting and Fusion, provides a wealth of new concepts, approaches and tools to study Rab proteins in the test tube and in living cells that will be of strong benefit to both established laboratories and new investigators in the field to elucidate Rab GTPase function in cellular development, differentiation and proliferation. Comprehensive overview of Rab GTPase phylogeny and systems biology Identification and characterization of Rab GEFs, GAPs and effectors General methodologies to study Rab GTPase function in vitro and in vivo using biochemical, molecular and microscopy approaches