Methods In Enzymology

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Peptide, Protein and Enzyme Design

Anonim
Peptide Protein and Enzyme Design Book PDF

Author : Anonim
Publisher : Academic Press
Page : 684 pages
File Size : 45,8 Mb
Release : 2016-08-27
Category : Medical
ISBN : 9780128054345

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Peptide, Protein and Enzyme Design by Anonim Pdf

De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. Continues the legacy of this premier serial with quality chapters on de novo enzyme design Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism

Guide to Protein Purification

Richard R Burgess,Murray P. Deutscher
Guide to Protein Purification Book PDF

Author : Richard R Burgess,Murray P. Deutscher
Publisher : Academic Press
Page : 915 pages
File Size : 45,7 Mb
Release : 2009-11-03
Category : Science
ISBN : 9780080923178

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Guide to Protein Purification by Richard R Burgess,Murray P. Deutscher Pdf

Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Methods of Soil Enzymology

Richard P. Dick
Methods of Soil Enzymology Book PDF

Author : Richard P. Dick
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 45,8 Mb
Release : 2020-01-22
Category : Technology & Engineering
ISBN : 9780891188544

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Methods of Soil Enzymology by Richard P. Dick Pdf

Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.

Enzyme Engineering and Evolution: General Methods

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Enzyme Engineering and Evolution General Methods Book PDF

Author : Anonim
Publisher : Academic Press
Page : 362 pages
File Size : 49,7 Mb
Release : 2020-09-05
Category : Science
ISBN : 9780128211502

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Enzyme Engineering and Evolution: General Methods by Anonim Pdf

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series

Methods in Systems Biology

Daniel Jameson,Malkhey Verma,Hans Westerhoff
Methods in Systems Biology Book PDF

Author : Daniel Jameson,Malkhey Verma,Hans Westerhoff
Publisher : Academic Press
Page : 775 pages
File Size : 48,9 Mb
Release : 2011-09-26
Category : Medical
ISBN : 9780123851185

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Methods in Systems Biology by Daniel Jameson,Malkhey Verma,Hans Westerhoff Pdf

Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research

Selenoprotein Structure and Function

Anonim
Selenoprotein Structure and Function Book PDF

Author : Anonim
Publisher : Academic Press
Page : 418 pages
File Size : 53,6 Mb
Release : 2022-01-28
Category : Science
ISBN : 9780323907361

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Selenoprotein Structure and Function by Anonim Pdf

Selenoprotein Structure and Function, Volume 662 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Identification of Selenoprotein O substrates using a biotinylated ATP analog, Selenium-encoded isotopic signature targeted profiling, Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters, Preparation of selenoprotein S by chemical ligation, Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells, SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond, Selenocysteine substitutions in thiyl radical enzymes, and much more. Additional chapters cover Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain, Metabolic labeling with radioactive selenium in zebrafish, Low pH isoTOP-ABPP to identify selenocysteines, Expression of selenoproteins via genetic code expansion in mammalian cells, Alpha-methyl selenocysteine as a tool for the study of selenoproteins, Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on Selenoprotein Structure and Function

Laboratory Methods in Enzymology: DNA

Anonim
Laboratory Methods in Enzymology DNA Book PDF

Author : Anonim
Publisher : Elsevier
Page : 405 pages
File Size : 47,5 Mb
Release : 2013-09-02
Category : Science
ISBN : 9780124199545

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Laboratory Methods in Enzymology: DNA by Anonim Pdf

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA

Enzymatic Plastic Degradation

Anonim
Enzymatic Plastic Degradation Book PDF

Author : Anonim
Publisher : Academic Press
Page : 500 pages
File Size : 41,8 Mb
Release : 2021-02-10
Category : Science
ISBN : 9780128220139

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Enzymatic Plastic Degradation by Anonim Pdf

Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Covers the latest research and technologies in enzymatic plastic degradation

Fragment Based Drug Design

Lawrence C. Kuo
Fragment Based Drug Design Book PDF

Author : Lawrence C. Kuo
Publisher : Academic Press
Page : 662 pages
File Size : 48,6 Mb
Release : 2011-03-09
Category : Medical
ISBN : 9780123812742

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Fragment Based Drug Design by Lawrence C. Kuo Pdf

There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD

The Resolution Revolution: Recent Advances In cryoEM

Anonim
The Resolution Revolution Recent Advances In cryoEM Book PDF

Author : Anonim
Publisher : Academic Press
Page : 486 pages
File Size : 51,7 Mb
Release : 2016-08-26
Category : Medical
ISBN : 9780128054352

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The Resolution Revolution: Recent Advances In cryoEM by Anonim Pdf

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their function Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Anonim
Laboratory Methods in Enzymology Cell Lipid and Carbohydrate Book PDF

Author : Anonim
Publisher : Academic Press
Page : 408 pages
File Size : 49,7 Mb
Release : 2013-10-30
Category : Science
ISBN : 9780124200944

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Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate by Anonim Pdf

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate

Contemporary Enzyme Kinetics and Mechanism

Daniel L. Purich
Contemporary Enzyme Kinetics and Mechanism Book PDF

Author : Daniel L. Purich
Publisher : Academic Press
Page : 567 pages
File Size : 48,9 Mb
Release : 1983-01-01
Category : Science
ISBN : 9781483214221

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Contemporary Enzyme Kinetics and Mechanism by Daniel L. Purich Pdf

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Methods in Non-Aqueous Enzymology

Munishwar N. Gupta
Methods in Non Aqueous Enzymology Book PDF

Author : Munishwar N. Gupta
Publisher : Birkhäuser
Page : 229 pages
File Size : 48,7 Mb
Release : 2013-12-01
Category : Science
ISBN : 9783034884723

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Methods in Non-Aqueous Enzymology by Munishwar N. Gupta Pdf

Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.

Ribonucleases, Part A: Functional Roles and Mechanisms of Action

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Ribonucleases Part A Functional Roles and Mechanisms of Action Book PDF

Author : Anonim
Publisher : Elsevier
Page : 771 pages
File Size : 44,9 Mb
Release : 2001-09-26
Category : Science
ISBN : 9780080496917

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Ribonucleases, Part A: Functional Roles and Mechanisms of Action by Anonim Pdf

This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Recombinant Protein Expression: Eukaryotic hosts

Anonim
Recombinant Protein Expression Eukaryotic hosts Book PDF

Author : Anonim
Publisher : Academic Press
Page : 384 pages
File Size : 51,6 Mb
Release : 2021-11-04
Category : Science
ISBN : 9780323907385

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Recombinant Protein Expression: Eukaryotic hosts by Anonim Pdf

Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression