Aqueous Two Phase Systems

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Aqueous Two-Phase Systems

Rajni Hatti-Kaul
Aqueous Two Phase Systems Book PDF

Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
Page : 442 pages
File Size : 51,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590285

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Aqueous Two-Phase Systems by Rajni Hatti-Kaul Pdf

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Partitioning In Aqueous Two – Phase System

Harry Walter
Partitioning In Aqueous Two Phase System Book PDF

Author : Harry Walter
Publisher : Elsevier
Page : 730 pages
File Size : 43,8 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323142915

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Partitioning In Aqueous Two – Phase System by Harry Walter Pdf

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Marco Rito-Palomares,Jorge Benavides
Aqueous Two Phase Systems for Bioprocess Development for the Recovery of Biological Products Book PDF

Author : Marco Rito-Palomares,Jorge Benavides
Publisher : Springer
Page : 191 pages
File Size : 41,5 Mb
Release : 2017-07-18
Category : Technology & Engineering
ISBN : 9783319593098

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Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products by Marco Rito-Palomares,Jorge Benavides Pdf

This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Aqueous Two-Phase Systems

Vangelis Anatolijs Xanthopoulos
Aqueous Two Phase Systems Book PDF

Author : Vangelis Anatolijs Xanthopoulos
Publisher : Unknown
Page : 0 pages
File Size : 53,7 Mb
Release : 2018
Category : Phase partition
ISBN : 1536142417

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Aqueous Two-Phase Systems by Vangelis Anatolijs Xanthopoulos Pdf

Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids. In Aqueous Two-Phase Systems: Properties, Functions and Advantages, the authors begin by discussing the benefits of these systems.The following chapter presents the main forms of the purification of lipases from aqueous biphasic systems, addressing and discussing the fundamentals for the formation, use and optimization of these systems applied in the biotechnological context. The authors address the basis of aqueous-biphasic systems, aqueous two-phase systems for enzymatic purification, purification of lipases and combined uses of aqueous two-phase systems.The most important experimental parameters that affect the efficiency of metal ions extraction in aqueous PEG-based two-phase systems are examined to provide a starting point in the design of a suitable system for the extraction of metal ions. Compared to the conventional solvent extraction, which uses toxic, flammable and volatile organic solvents and can be quite expensive, the extraction in aqueous PEG-based two-phase systems is considered a more environmental friendly and economically viable method.Also in this compilation, an overview about application of ATPSs in microstructured devices is provided. Microstructured devices offer potential benefits due to well-defined high specific interfacial areas available for heat and mass transfer. These areas increase transfer rate and enhance yield, selectivity and process control. The application of ATPSs in Micro Total Analysis Systems is described as well.

Aqueous Two-Phase Partitioning

Boris Y. Zaslavsky
Aqueous Two Phase Partitioning Book PDF

Author : Boris Y. Zaslavsky
Publisher : CRC Press
Page : 720 pages
File Size : 46,7 Mb
Release : 1994-11-15
Category : Science
ISBN : 0824794613

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Aqueous Two-Phase Partitioning by Boris Y. Zaslavsky Pdf

Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.

Intensification of Biobased Processes

Andrzej Gorak,Andrzej Stankiewicz
Intensification of Biobased Processes Book PDF

Author : Andrzej Gorak,Andrzej Stankiewicz
Publisher : Royal Society of Chemistry
Page : 518 pages
File Size : 52,8 Mb
Release : 2018-06-18
Category : Science
ISBN : 9781788010320

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Intensification of Biobased Processes by Andrzej Gorak,Andrzej Stankiewicz Pdf

In recent years bioprocessing has increased in popularity and importance, however, bioprocessing still poses various important techno-economic and environmental challenges, such as product yields, excessive energy consumption for separations in highly watery systems, batch operation or the downstream processing bottlenecks in the production of biopharmaceutical products. Many of those challenges can be addressed by application of different process intensification technologies discussed in the present book. The first book dedicated entirely to this area, Intensification of Biobased Processesprovides a comprehensive overview of modern process intensification technologies used in bioprocessing. The book focusses on four different categories of biobased products: bio-fuels and platform chemicals; cosmeceuticals; food products; and polymers and advanced materials. It will cover various intensification aspects of the processes concerned, including (bio)reactor intensification; intensification of separation, recovery and formulation operations; and process integration. This is an invaluable source of information for researchers and industrialists working in chemical engineering, biotechnology and process engineering.

Ionic-Liquid-Based Aqueous Biphasic Systems

Mara G. Freire
Ionic Liquid Based Aqueous Biphasic Systems Book PDF

Author : Mara G. Freire
Publisher : Springer
Page : 315 pages
File Size : 45,9 Mb
Release : 2016-10-05
Category : Technology & Engineering
ISBN : 9783662528754

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Ionic-Liquid-Based Aqueous Biphasic Systems by Mara G. Freire Pdf

This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.

Protein Structure Analysis

Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold
Protein Structure Analysis Book PDF

Author : Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold
Publisher : Springer Science & Business Media
Page : 311 pages
File Size : 53,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642592195

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Protein Structure Analysis by Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold Pdf

"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Aqueous Two-Phase Systems

Harry Walter,Göte Johansson
Aqueous Two Phase Systems Book PDF

Author : Harry Walter,Göte Johansson
Publisher : Elsevier
Page : 768 pages
File Size : 53,7 Mb
Release : 1994-04-18
Category : Nature
ISBN : 0121821293

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Aqueous Two-Phase Systems by Harry Walter,Göte Johansson Pdf

General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.

Extractive Bioconversions

B. Mattiasson
Extractive Bioconversions Book PDF

Author : B. Mattiasson
Publisher : CRC Press
Page : 348 pages
File Size : 43,8 Mb
Release : 1990-11-30
Category : Medical
ISBN : 0824782720

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Extractive Bioconversions by B. Mattiasson Pdf

Contributors from European and US universities and corporations review recent developments in the integration of downstream processing and bioconversion, describing their experience with many separation technologies, including some still in the experimental stage. The topics include the construction

The Giant Vesicle Book

Rumiana Dimova,Carlos Marques
The Giant Vesicle Book Book PDF

Author : Rumiana Dimova,Carlos Marques
Publisher : CRC Press
Page : 1144 pages
File Size : 50,8 Mb
Release : 2019-11-19
Category : Science
ISBN : 9781351648554

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The Giant Vesicle Book by Rumiana Dimova,Carlos Marques Pdf

Giant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features • A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications • A start-up package of theoretical and experimental information for newcomers in the field • Extensive protocols for establishing the required preparations and assays • Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls • Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques • Handy tables with data and structures for ready reference

The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering

John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum
The Science and Application of Aqueous Two Phase Systems and Liquid Liquid Phase Separation in Biotechnology and Bioengineering Book PDF

Author : John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum
Publisher : Frontiers Media SA
Page : 117 pages
File Size : 45,7 Mb
Release : 2020-01-13
Category : Electronic
ISBN : 9782889633562

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The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering by John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum Pdf

The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.

Partition of Cell Particles and Macromolecules

Per-Ake Albertsson
Partition of Cell Particles and Macromolecules Book PDF

Author : Per-Ake Albertsson
Publisher : Wiley-Interscience
Page : 346 pages
File Size : 52,7 Mb
Release : 1986-03-28
Category : Science
ISBN : 0471828203

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Partition of Cell Particles and Macromolecules by Per-Ake Albertsson Pdf

Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.

Liquid Biphasic System

Pau Loke Show,Sze Ying Lee,Kit Wayne Chew
Liquid Biphasic System Book PDF

Author : Pau Loke Show,Sze Ying Lee,Kit Wayne Chew
Publisher : Elsevier
Page : 330 pages
File Size : 42,5 Mb
Release : 2020-11-28
Category : Technology & Engineering
ISBN : 9780128223987

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Liquid Biphasic System by Pau Loke Show,Sze Ying Lee,Kit Wayne Chew Pdf

Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings. Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. Provides information and examples of advanced separations in a single source Includes detailed descriptions of novel bioseparation systems Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries

Bijels

Paul S Clegg
Bijels Book PDF

Author : Paul S Clegg
Publisher : Royal Society of Chemistry
Page : 266 pages
File Size : 51,9 Mb
Release : 2020-03-27
Category : Science
ISBN : 9781788015202

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Bijels by Paul S Clegg Pdf

Bicontinuous interfacially jammed emulsion gels, now commonly termed 'bijels', are a class of soft materials, in which interpenetrating, continuous domains of two immiscible fluids are maintained in a rigid arrangement by a jammed layer of colloidal particles at their interface. Such gels have unusual material properties that promise exciting applications across diverse fields from energy materials and catalysis, to food science. This is the first book on the subject and provides the reader with a fundamental introduction. Edited by a recognised authority on bijels, the reader will learn about the bijel and its formation. Bringing together current understanding, this book aims to bring the potential application of bijels to diverse materials challenges closer to fruition. This is a must-have resource for anyone working in soft matter and applied fields.