Capillary Electrophoresis And Microchip Capillary Electrophoresis

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Capillary Electrophoresis and Microchip Capillary Electrophoresis

Author : Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho
Publisher : John Wiley & Sons
Page : 638 pages
File Size : 47,8 Mb
Release : 2013-02-01
Category : Science
ISBN : 9781118529997

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Capillary Electrophoresis and Microchip Capillary Electrophoresis by Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho Pdf

Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications.

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques

Author : James P. Landers
Publisher : CRC Press
Page : 1598 pages
File Size : 44,6 Mb
Release : 2007-12-18
Category : Medical
ISBN : 9781420004953

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Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques by James P. Landers Pdf

Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.

Microchip Capillary Electrophoresis Protocols

Author : Ann Van Schepdael
Publisher : Unknown
Page : 200 pages
File Size : 44,7 Mb
Release : 2015
Category : Biochemistry
ISBN : 1493923536

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Microchip Capillary Electrophoresis Protocols by Ann Van Schepdael Pdf

Annotation In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis.

Microchip Capillary Electrophoresis

Author : Charles Henry
Publisher : Humana
Page : 238 pages
File Size : 44,6 Mb
Release : 2006-03-01
Category : Science
ISBN : 1588292932

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Microchip Capillary Electrophoresis by Charles Henry Pdf

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Microchip Capillary Electrophoresis

Author : Charles Henry
Publisher : Springer Science & Business Media
Page : 238 pages
File Size : 48,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450768

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Microchip Capillary Electrophoresis by Charles Henry Pdf

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Capillary Gel Electrophoresis

Author : Andras Guttman,László Hajba
Publisher : Newnes
Page : 391 pages
File Size : 54,9 Mb
Release : 2021-12-04
Category : Science
ISBN : 9780080931357

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Capillary Gel Electrophoresis by Andras Guttman,László Hajba Pdf

Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Analytical Techniques in Forensic Science

Author : Rosalind Wolstenholme,Sue Jickells,Shari Forbes
Publisher : John Wiley & Sons
Page : 464 pages
File Size : 52,5 Mb
Release : 2021-01-26
Category : Medical
ISBN : 9781119978282

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Analytical Techniques in Forensic Science by Rosalind Wolstenholme,Sue Jickells,Shari Forbes Pdf

An in-depth text that explores the interface between analytical chemistry and trace evidence Analytical Techniques in Forensic Science is a comprehensive guide written in accessible terms that examines the interface between analytical chemistry and trace evidence in forensic science. With contributions from noted experts on the topic, the text features a detailed introduction analysis in forensic science and then subsequent chapters explore the laboratory techniques grouped by shared operating principles. For each technique, the authors incorporate specific theory, application to forensic analytics, interpretation, forensic specific developments, and illustrative case studies. Forensic techniques covered include UV-Vis and vibrational spectroscopy, mass spectrometry and gas and liquid chromatography. The applications reviewed include evidence types such as fibers, paint, drugs and explosives. The authors highlight data collection, subsequent analysis, what information has been obtained and what this means in the context of a case. The text shows how analytical chemistry and trace evidence can problem solve the nature of much of forensic analysis. This important text: Puts the focus on trace evidence and analytical science Contains case studies that illustrate theory in practice Includes contributions from experts on the topics of instrumentation, theory, and case examples Explores novel and future applications for analytical techniques Written for undergraduate and graduate students in forensic chemistry and forensic practitioners and researchers, Analytical Techniques in Forensic Science offers a text that bridges the gap between introductory textbooks and professional level literature.

Capillary Electrophoresis and Microchip Technology, Second Edition

Author : Norberto A. Guzman,Ernest Kenndler
Publisher : CRC Press
Page : 900 pages
File Size : 47,8 Mb
Release : 2008-12
Category : Science
ISBN : 1574447394

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Capillary Electrophoresis and Microchip Technology, Second Edition by Norberto A. Guzman,Ernest Kenndler Pdf

As capillary electrophoresis transforms into a mature technology in nearly every field of chemistry and biology, Capillary Electrophoresis and Microchip Technology, Second Edition details the latest concepts, instrumentation, and applications of capillary electrophoresis (CE). Building on the success of its predecessor, this edition features expanded coverage of the Human Genome and the Human Proteomic Projects. It includes expanded instrumentation and application sections, with topics ranging from mass spectrometry to microfabrication tools and microchip design options. The text offers an overview of miniaturization techniques and modes of CE. It also discusses capillary electrochormatography and the background electrolyte system.

Microchip Capillary Electrophoresis Protocols

Author : Ann Van Schepdael
Publisher : Humana Press
Page : 200 pages
File Size : 41,5 Mb
Release : 2015-02-12
Category : Science
ISBN : 1493923544

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Microchip Capillary Electrophoresis Protocols by Ann Van Schepdael Pdf

In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis.

Microfluidic Chip-Capillary Electrophoresis Devices

Author : Ying Sing Fung,Qidan Chen,Fuying Du,Wenpeng Guo,Tongmei Ma,Zhou Nie,Hui Sun,Ruige Wu,Wenfeng Zhao
Publisher : CRC Press
Page : 396 pages
File Size : 51,5 Mb
Release : 2015-08-18
Category : Science
ISBN : 9781482251654

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Microfluidic Chip-Capillary Electrophoresis Devices by Ying Sing Fung,Qidan Chen,Fuying Du,Wenpeng Guo,Tongmei Ma,Zhou Nie,Hui Sun,Ruige Wu,Wenfeng Zhao Pdf

Capillary electrophoresis (CE) and microfluidic chip (MC) devices are relatively mature technologies, but this book demonstrates how they can be integrated into a single, revolutionary device that can provide on-site analysis of samples when laboratory services are unavailable. By introducing the combination of CE and MC technology, Microfluidic Ch

Capillary Electrophoresis - Mass Spectrometry (CE-MS)

Author : Gerhardus de Jong
Publisher : John Wiley & Sons
Page : 364 pages
File Size : 48,6 Mb
Release : 2016-09-13
Category : Science
ISBN : 9783527339242

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Capillary Electrophoresis - Mass Spectrometry (CE-MS) by Gerhardus de Jong Pdf

This monograph offers the reader a complete overview on both principles and applications of CE-MS. Starting with an introductory chapter on detection in CE, also related and more specialized techniques such as electrophoretic and chromatographic preconcentration are discussed. A special emphasis is put on CE-MS interfaces, which are described in detail. In a separate chapter, attention is paid to sheath-liquid interfacing. The developments and possibilities of microchip CE-MS are also described. Applications to all relevant areas are discussed in distinct chapters, each written by experts in the respective fields. Besides applications in pharmaceutical analysis and bioanalysis, recent implementations in food science, forensic analysis, analysis of intact proteins, metabolomics and proteomics are highlighted. MS is a perfectly appropriate detection system for CE, as efficient separation is coupled to sensitive and selection detection. Moreover, MS can provide structure information on the separated compounds. CE-MS has now been developed into a strong hyphenated system complementary to LC-MS. This monograph is an unique source of knowledge for everyone dealing with and interested in CE-MS.

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition

Author : James P. Landers
Publisher : CRC Press
Page : 1592 pages
File Size : 51,5 Mb
Release : 2007-12-18
Category : Medical
ISBN : 0849333296

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Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition by James P. Landers Pdf

Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.

Electromigration Techniques

Author : Boguslaw Buszewski,Ewelina Dziubakiewicz,Michal Szumski
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 42,7 Mb
Release : 2013-04-23
Category : Technology & Engineering
ISBN : 9783642350436

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Electromigration Techniques by Boguslaw Buszewski,Ewelina Dziubakiewicz,Michal Szumski Pdf

The book provides the broad knowledge on electromigration techniques including: theory of CE, description of instrumentation, theory and practice in micellar electrokinetic chromatography, isotachophoresis, capillary isoelectric focusing, capillary and planar electrochromatography (including description of instrumentation and packed and monolithic column preparation), 2D-gel electrophoresis (including sample preparation) and lab-on-a-chip systems. The book also provides the most recent examples of applications including food, environmental, pharmaceutical analysis as well as proteomics.

Capillary Electrophoresis of Proteins and Peptides

Author : Mark A. Strege,Avinash L. Lagu
Publisher : Springer Science & Business Media
Page : 340 pages
File Size : 46,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592597987

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Capillary Electrophoresis of Proteins and Peptides by Mark A. Strege,Avinash L. Lagu Pdf

Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.

Biological Applications of Microfluidics

Author : Frank A. Gomez
Publisher : John Wiley & Sons
Page : 562 pages
File Size : 46,5 Mb
Release : 2008-02-15
Category : Science
ISBN : 9780470074831

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Biological Applications of Microfluidics by Frank A. Gomez Pdf

Microfluidics has numerous potential applications in biotechnology, pharmaceuticals, the life sciences, defense, public health, and agriculture. This book details recent advances in the biological applications of microfluidics, including cell sorting, DNA sequencing on-a-chip, microchip capillary electrophoresis, and synthesis on a microfluidic format. It covers microfabricated LOC technologies, advanced microfluidic tools, microfluidic culture platforms for stem cell and neuroscience research, and more. This is an all-in-one, hands-on resource for analytical chemists and researchers and an excellent text for students.