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Author : David Thompson
Publisher : Createspace Independent Publishing Platform
Page : 170 pages
File Size : 49,5 Mb
Release : 2011-06-14
Category : Electronic
ISBN : 1463530080
A laboratory manual for an undergraduate-level cell and molecular biology course.
Author : David A. Thompson
Publisher : Unknown
Page : 164 pages
File Size : 48,7 Mb
Release : 2009-07-12
Category : Electronic
ISBN : 1442196092
cell and molecular biology laboratory manual 2009
Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
Publisher : Academic Press
Page : 294 pages
File Size : 43,7 Mb
Release : 2019-03-05
Category : Science
ISBN : 9780128157756
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : K. V. CHAITANYA
Publisher : PHI Learning Pvt. Ltd.
Page : 156 pages
File Size : 49,8 Mb
Release : 2013-06-21
Category : Science
ISBN : 9788120348004
This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.
Author : Stefan Surzycki
Publisher : John Wiley & Sons
Page : 246 pages
File Size : 53,7 Mb
Release : 2008-04-15
Category : Science
ISBN : 9780470999240
Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Author : Gerald Karp
Publisher : Wiley
Page : 1120 pages
File Size : 43,5 Mb
Release : 2013-04-18
Category : Science
ISBN : 1118754034
Never HIGHLIGHT a Book Again! Includes all testable terms, concepts, persons, places, and events. Cram101 Just the FACTS101 studyguides gives all of the outlines, highlights, and quizzes for your textbook with optional online comprehensive practice tests. Only Cram101 is Textbook Specific. Accompanies: 9781118754030. This item is printed on demand.
Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 50,8 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : Melody S. Clark
Publisher : Springer Science & Business Media
Page : 551 pages
File Size : 52,8 Mb
Release : 2013-11-27
Category : Science
ISBN : 9783642878732
Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author : Thomas A. Lonergan,Sherwood Githens,Gary Braedt
Publisher : Unknown
Page : 150 pages
File Size : 50,6 Mb
Release : 1996-01-01
Category : Electronic
ISBN : 1890264016
Author : Julio E. Celis
Publisher : Elsevier
Page : 632 pages
File Size : 40,6 Mb
Release : 2006
Category : Science
ISBN : 9780121647308
This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) Organelle and Cellular Structures, Assays (Volume 2) Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) Indispensable bench companion for every life science laboratory Provides the latest information on the plethora of technologies needed to tackle complex biological problems Includes numerous illustrations, some in full color, supporting steps and results
Author : Quincy Quick
Publisher : Unknown
Page : 128 pages
File Size : 49,8 Mb
Release : 2009-07-01
Category : Electronic
ISBN : 0615310168
This book is a practical Undergraduate Cellular and Molecular Biology Laboratory manual with an emphasis on fundamental techniques used in Cell Biology
Author : Karp
Publisher : Unknown
Page : 128 pages
File Size : 50,6 Mb
Release : 2002-08-01
Category : Electronic
ISBN : 0471273899
Author : Anthony Contento
Publisher : Wiley Global Education
Page : 288 pages
File Size : 40,6 Mb
Release : 2012-10-26
Category : Science
ISBN : 9781118473955
The Contento Experimental Cell Biology Lab Book is a modular design that matches the topics discussed in Karp’s textbook. The manual itself consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text. There are three possible designs of the lab book, based on the instructor’s needs. These designs focus on either Techniques, Concepts, or Organelles. The procedures of the 30+ experiments remain standard and unchanged in all designs of the lab book. Special Overview pages, Discussion Questions and Datasheets bookend the procedures in order to create each of the possible textbook designs.This gives instructors flexibility to create a lab book that suits their lecture course curriculum, their experience, and available equipment and supplies.
Author : Gerald Karp
Publisher : Wiley
Page : 128 pages
File Size : 41,7 Mb
Release : 2014-11-05
Category : Science
ISBN : 1119071674
Author : S. K. Gakhar,Monika Miglani,Ashwani Kumar
Publisher : Unknown
Page : 0 pages
File Size : 46,5 Mb
Release : 2013-09-30
Category : Electronic
ISBN : 9382332308
The objective of this text is to train young teachers from colleges and research institutions so that they can advance their research in various fields of biology. It will also help students at BSc and MSc level to learn the techniques involved in molecular biology. The book contains four chapters providing step-by-step protocols. In addition, it has general instructions for safety procedures.