Cell Culture Engineering Vi

Author : Michael J. Betenbaugh,Jeffrey J. Chalmers,Rob Arathoon,Frank W.R. Chaplen,Alison J. Mastrangelo
Publisher : Springer Science & Business Media
Page : 229 pages
File Size : 48,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401147866

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Cell Culture Engineering VI by Michael J. Betenbaugh,Jeffrey J. Chalmers,Rob Arathoon,Frank W.R. Chaplen,Alison J. Mastrangelo Pdf

The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings. Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.

Author : Wei-Shou Hu
Publisher : CRC Press
Page : 466 pages
File Size : 53,5 Mb
Release : 2020-03-06
Category : Science
ISBN : 9781498762861

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Cell Culture Bioprocess Engineering, Second Edition by Wei-Shou Hu Pdf

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

Author : Gyun Min Lee,Helene Faustrup Kildegaard
Publisher : John Wiley & Sons
Page : 436 pages
File Size : 50,9 Mb
Release : 2020-01-13
Category : Science
ISBN : 9783527343348

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Cell Culture Engineering by Gyun Min Lee,Helene Faustrup Kildegaard Pdf

Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

Author : Sadettin Ozturk,Wei-Shou Hu
Publisher : CRC Press
Page : 788 pages
File Size : 53,9 Mb
Release : 2005-08-30
Category : Medical
ISBN : 9780849351068

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Cell Culture Technology for Pharmaceutical and Cell-Based Therapies by Sadettin Ozturk,Wei-Shou Hu Pdf

Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel

Author : Wei-Shu Hu
Publisher : Springer
Page : 0 pages
File Size : 40,7 Mb
Release : 2010-11-18
Category : Science
ISBN : 3642070515

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Cell Culture Engineering by Wei-Shu Hu Pdf

Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.

Author : Mattheus F. A. Goosen
Publisher : CRC Press
Page : 260 pages
File Size : 53,7 Mb
Release : 2020-07-24
Category : Science
ISBN : 9781000105667

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Insect Cell Culture Engineering by Mattheus F. A. Goosen Pdf

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Author : B.C. Buckland
Publisher : Springer Science & Business Media
Page : 366 pages
File Size : 55,8 Mb
Release : 2013-06-29
Category : Science
ISBN : 9789401102575

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Cell Culture Engineering IV by B.C. Buckland Pdf

Cell Culture Engineering IV, Improvements of Human Health covers the latest approaches to improving the cell host through improved understanding of the molecular biology, the development of novel vaccines, approaches to bioreactor design and operation, monitoring techniques in process control and quality related topics. The work was carefully put together as one result of the Cell Culture Engineering IV Meeting held in San Diego, U.S.A. in 1994, however, the book may not be perceived as a proceedings volume - the criteria of the book series apply. For cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment, as well as in (biotechnology or pharmaceutical) industry.

Author : Wei-Shou Hu
Publisher : CRC Press
Page : 453 pages
File Size : 44,5 Mb
Release : 2020
Category : Medical
ISBN : 0429546599

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Cell Culture Bioprocess Engineering, Second Edition by Wei-Shou Hu Pdf

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

Author : Wei-Shu Hu,Richard Biener
Publisher : Unknown
Page : 192 pages
File Size : 51,8 Mb
Release : 2006-08-23
Category : Science
ISBN : UOM:39015066862791

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Cell Culture Engineering by Wei-Shu Hu,Richard Biener Pdf

Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.

Author : Ralf Pörtner
Publisher : Springer Nature
Page : 552 pages
File Size : 42,7 Mb
Release : 2022-02-20
Category : Medical
ISBN : 9783030798710

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Cell Culture Engineering and Technology by Ralf Pörtner Pdf

This contributed volume is dedicated towards the progress achieved within the last years in all areas of Cell Culture Engineering and Technology. It comprises contributions of active researchers in the field of cell culture development for the production of recombinant proteins, cell line development, cell therapy and gene therapy, with consideration of media development, process scale-up, reactor design, monitoring and control and model-assisted strategies for process design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. This book is conceived for graduate students, postdoctoral fellows and researchers interested in the latest developments in Cell Engineering.

Author : Barry C. Buckland
Publisher : Unknown
Page : 380 pages
File Size : 53,7 Mb
Release : 1994
Category : Biotechnology
ISBN : OCLC:38997743

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Cell Culture Engineering IV by Barry C. Buckland Pdf

Author : Gordana Vunjak-Novakovic,R. Ian Freshney
Publisher : John Wiley & Sons
Page : 528 pages
File Size : 46,8 Mb
Release : 2006-03-31
Category : Science
ISBN : 9780471741800

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Culture of Cells for Tissue Engineering by Gordana Vunjak-Novakovic,R. Ian Freshney Pdf

Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

Author : Hans-Peter Meyer,Diego Schmidhalter
Publisher : John Wiley & Sons
Page : 642 pages
File Size : 54,8 Mb
Release : 2014-07-30
Category : Science
ISBN : 9783527683352

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Industrial Scale Suspension Culture of Living Cells by Hans-Peter Meyer,Diego Schmidhalter Pdf

The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.

Author : E. Lindner-Olsson,N. Chatzissavidou,E. Lüllau
Publisher : Springer Science & Business Media
Page : 657 pages
File Size : 42,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401003698

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Animal Cell Technology: From Target to Market by E. Lindner-Olsson,N. Chatzissavidou,E. Lüllau Pdf

Proceedings of the 17th ESACT Meeting June 10-14, 2001, Tylösand, Sweden

Author : Mattheus F. A. Goosen
Publisher : CRC Press
Page : 264 pages
File Size : 48,7 Mb
Release : 2020-07-24
Category : Science
ISBN : 9781000148879

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Insect Cell Culture Engineering by Mattheus F. A. Goosen Pdf

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.