Cell Free Protein Expression

Author : Ashty S. Karim,Michael C. Jewett
Publisher : Unknown
Page : 435 pages
File Size : 54,8 Mb
Release : 2022
Category : Biology
ISBN : 1071619985

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Cell-Free Gene Expression by Ashty S. Karim,Michael C. Jewett Pdf

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

Author : James R. Swartz
Publisher : Springer Science & Business Media
Page : 213 pages
File Size : 50,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642593376

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Cell-Free Protein Expression by James R. Swartz Pdf

Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems", edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.

Author : W. Antoni Kudlicki
Publisher : CRC Press
Page : 242 pages
File Size : 43,8 Mb
Release : 2007-11-27
Category : Science
ISBN : 9781498713290

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Cell-Free Protein Expression by W. Antoni Kudlicki Pdf

Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica

Author : Alexander S. Spirin,James R. Swartz
Publisher : John Wiley & Sons
Page : 272 pages
File Size : 41,6 Mb
Release : 2007-12-03
Category : Science
ISBN : 3527316493

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Cell-free Protein Synthesis by Alexander S. Spirin,James R. Swartz Pdf

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Author : Alexander S. Spirin,James R. Swartz
Publisher : John Wiley & Sons
Page : 262 pages
File Size : 46,6 Mb
Release : 2014-08-15
Category : Science
ISBN : 9783527691500

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Cell-free Protein Synthesis by Alexander S. Spirin,James R. Swartz Pdf

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Author : A.S. Spirin
Publisher : Springer Science & Business Media
Page : 254 pages
File Size : 40,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642593796

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Cell-Free Translation Systems by A.S. Spirin Pdf

This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.

Author : Jian Li,Yong-Chan Kwon,Yuan Lu,Simon J. Moore
Publisher : Frontiers Media SA
Page : 205 pages
File Size : 43,6 Mb
Release : 2022-01-13
Category : Science
ISBN : 9782889740802

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Cell-Free Synthetic Biology by Jian Li,Yong-Chan Kwon,Yuan Lu,Simon J. Moore Pdf

Author : Anonim
Publisher : Academic Press
Page : 462 pages
File Size : 43,9 Mb
Release : 2021-10-29
Category : Science
ISBN : 9780323901475

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Recombinant Protein Expression: Prokaryotic hosts and cell-free systems by Anonim Pdf

Recombinant Protein Expression, Part A, Volume 659 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression

Author : Seok Hoon Hong
Publisher : MDPI
Page : 152 pages
File Size : 41,9 Mb
Release : 2020-01-07
Category : Science
ISBN : 9783039280223

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Cell-Free Synthetic Biology by Seok Hoon Hong Pdf

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.

Author : Ron Milo,Rob Phillips
Publisher : Garland Science
Page : 400 pages
File Size : 43,7 Mb
Release : 2015-12-07
Category : Science
ISBN : 9781317230694

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Cell Biology by the Numbers by Ron Milo,Rob Phillips Pdf

A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid

Author : Yaeta Endo,Kazuyuki Takai,Takuya Ueda
Publisher : Humana
Page : 261 pages
File Size : 55,6 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493956981

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Cell-Free Protein Production by Yaeta Endo,Kazuyuki Takai,Takuya Ueda Pdf

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 44,9 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : S. J. Higgins,B. D. Hames
Publisher : OUP Oxford
Page : 303 pages
File Size : 50,5 Mb
Release : 1999-05-20
Category : Science
ISBN : 9780191565588

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Protein Expression by S. J. Higgins,B. D. Hames Pdf

Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.

Author : Christoph Wittmann,James C. Liao
Publisher : John Wiley & Sons
Page : 789 pages
File Size : 45,5 Mb
Release : 2017-04-10
Category : Science
ISBN : 9783527341795

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Industrial Biotechnology by Christoph Wittmann,James C. Liao Pdf

The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses. Of interest to biotechnologists and microbiologists, as well as those working in the biotechnological, chemical, food and pharmaceutical industries.The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses. Of interest to biotechnologists and microbiologists, as well as those working in the biotechnological, chemical, food and pharmaceutical industries.

Author : Anonim
Publisher : Academic Press
Page : 350 pages
File Size : 43,7 Mb
Release : 2009-07-24
Category : Science
ISBN : 0080921639

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Non-Natural Amino Acids by Anonim Pdf

By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods