Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 45,9 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181
Molecular Biology of The Cell
Bruce Alberts
Protein Expression
Protein Expression Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Protein Expression book. This book definitely worth reading, it is an incredibly well-written.
Recombinant Gene Expression
Paulina Balbas,Argelia Lorence
Author : Paulina Balbas,Argelia Lorence
Publisher : Springer Science & Business Media
Page : 505 pages
File Size : 41,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592597741
Recombinant Gene Expression by Paulina Balbas,Argelia Lorence Pdf
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Textbook on Cloning, Expression and Purification of Recombinant Proteins
Kakoli Bose
Author : Kakoli Bose
Publisher : Springer Nature
Page : 315 pages
File Size : 44,5 Mb
Release : 2022-01-25
Category : Medical
ISBN : 9789811649875
Textbook on Cloning, Expression and Purification of Recombinant Proteins by Kakoli Bose Pdf
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Protein Expression in Mammalian Cells
James L Hartley
Author : James L Hartley
Publisher : Humana Press
Page : 276 pages
File Size : 53,7 Mb
Release : 2011-10-12
Category : Science
ISBN : 1617793531
Protein Expression in Mammalian Cells by James L Hartley Pdf
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
High Throughput Protein Expression and Purification
Sharon A. Doyle
Author : Sharon A. Doyle
Publisher : Humana Press
Page : 0 pages
File Size : 48,6 Mb
Release : 2010-11-19
Category : Science
ISBN : 1617378216
High Throughput Protein Expression and Purification by Sharon A. Doyle Pdf
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
Production of Recombinant Proteins
Gerd Gellissen
Author : Gerd Gellissen
Publisher : John Wiley & Sons
Page : 429 pages
File Size : 53,6 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527604418
Production of Recombinant Proteins by Gerd Gellissen Pdf
While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
Recombinant Protein Expression in Mammalian Cells
David L. Hacker
Author : David L. Hacker
Publisher : Humana Press
Page : 311 pages
File Size : 43,5 Mb
Release : 2018-09-03
Category : Science
ISBN : 1493987291
Recombinant Protein Expression in Mammalian Cells by David L. Hacker Pdf
This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Protein Expression
S. J. Higgins,B. D. Hames
Author : S. J. Higgins,B. D. Hames
Publisher : OUP Oxford
Page : 303 pages
File Size : 45,7 Mb
Release : 1999-05-20
Category : Science
ISBN : 9780191565588
Protein Expression by S. J. Higgins,B. D. Hames Pdf
Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
Recombinant Protein Expression: Eukaryotic hosts
Anonim
Author : Anonim
Publisher : Academic Press
Page : 384 pages
File Size : 52,9 Mb
Release : 2021-11-04
Category : Science
ISBN : 9780323907385
Recombinant Protein Expression: Eukaryotic hosts by Anonim Pdf
Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression
Membrane Protein Protocols
Barry S. Selinsky
Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 48,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592594009
Membrane Protein Protocols by Barry S. Selinsky Pdf
Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Gene Expression Systems
Joseph M. Fernandez,James P. Hoeffler
Author : Joseph M. Fernandez,James P. Hoeffler
Publisher : Elsevier
Page : 493 pages
File Size : 45,8 Mb
Release : 1998-12-21
Category : Science
ISBN : 9780080532356
Gene Expression Systems by Joseph M. Fernandez,James P. Hoeffler Pdf
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method
Protein Expression Technologies
François Baneyx
Author : François Baneyx
Publisher : Garland Science
Page : 546 pages
File Size : 44,6 Mb
Release : 2004
Category : Medical
ISBN : 0954523253
Protein Expression Technologies by François Baneyx Pdf
Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus.
Cell-Free Protein Expression
W. Antoni Kudlicki
Author : W. Antoni Kudlicki
Publisher : CRC Press
Page : 242 pages
File Size : 43,7 Mb
Release : 2007-11-27
Category : Science
ISBN : 9781498713290
Cell-Free Protein Expression by W. Antoni Kudlicki Pdf
Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica
Recombinant protein expression in microbial systems
Eduardo A. Ceccarelli,Germán L. Rosano
Author : Eduardo A. Ceccarelli,Germán L. Rosano
Publisher : Frontiers E-books
Page : 103 pages
File Size : 53,6 Mb
Release : 2014-10-02
Category : Biotechnology
ISBN : 9782889192946
Recombinant protein expression in microbial systems by Eduardo A. Ceccarelli,Germán L. Rosano Pdf
With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.
Cell-Free Protein Expression
James R. Swartz
Author : James R. Swartz
Publisher : Springer Science & Business Media
Page : 213 pages
File Size : 49,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642593376
Cell-Free Protein Expression by James R. Swartz Pdf
Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems", edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.
