Cellular In Vitro Testing

Author : John Haycock,Arti Ahluwalia,J. Malcolm Wilkinson
Publisher : CRC Press
Page : 194 pages
File Size : 55,8 Mb
Release : 2014-08-27
Category : Medical
ISBN : 9789814364973

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Cellular In Vitro Testing by John Haycock,Arti Ahluwalia,J. Malcolm Wilkinson Pdf

Growing cells in 2D under static conditions has long been the gold standard of cell culture, despite this method not being representative of the complex in vivo environment. The use of animal models also has clear ethical and scientific limitations, and increasingly the 3Rs (replacement, refinement, reduction) in relation to animal models are being integrated into the modern-day scientific practice. Focusing on new 3D in vitro methods now available to researchers, this book brings together examples of leading-edge work being conducted internationally for improving in vitro cell culture methods, in particular the use of systems for enabling cell culture under laminar flow and the use of 3D scaffolds for providing cells with a structure which replicates the function of the extracellular matrix and encouraging interactions more akin to an in vivo environment.

Author : Barry R. Bloom,Philip R. Glade
Publisher : Academic Press
Page : 602 pages
File Size : 43,7 Mb
Release : 2014-06-28
Category : Science
ISBN : 9781483277592

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In Vitro Methods in Cell-Mediated Immunity by Barry R. Bloom,Philip R. Glade Pdf

In Vitro Methods in Cell-Mediated Immunity focuses on methods for approaching cell-mediated immune responses in vitro. This book provides, in simplified in vitro systems, a basis for understanding the mechanism of the in vivo response and discusses useful and reliable in vitro tests for cell-mediated immune responses in humans, where in vivo testing is often not possible. The topics discussed include factors and activities produced in vitro by lymphocytes; biological implications of in vitro phenomena; and requirements and prospects for improved methodology. The leucocyte migration technique for in vitro detection of cellular hypersensitivity in man; proliferation of human blood lymphocytes stimulated by antigen in vitro; and virus plaque assay for antigen-sensitive are also elaborated in this text. This publication is a good reference for microbiologist and immunologists, including medical students researching on in vitro models for cell-mediated immune reactions.

Author : Jose V. Castell,Maria Jose Gmez-Lechn
Publisher : Elsevier
Page : 483 pages
File Size : 46,9 Mb
Release : 1996-10-04
Category : Medical
ISBN : 9780080534602

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In Vitro Methods in Pharmaceutical Research by Jose V. Castell,Maria Jose Gmez-Lechn Pdf

In Vitro Methods in Pharmaceutical Research provides a comprehensive guide to laboratory techniques for evaluating in vitro organ toxicity using cellular models. Step-by-step practical tips on how to perform and interpret assays for drug metabolism and toxicity assessment are provided, along with a comparison of different techniques available. It is a welcome addition to the literature at a time when interest is growing in cellular in vitro models for toxicology and pharmacology studies. Meets the continuing demand for information in this field Compares In Vitro techniques with other methods Describes cell-culture methods used to investigate toxicity in cells derived from different organs Includes contributions by leading experts in the field

Author : S. A. Brown
Publisher : ASTM International
Page : 182 pages
File Size : 41,7 Mb
Release : 1983
Category : Science
ISBN : 0803102496

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Cell-culture Test Methods by S. A. Brown Pdf

Author : Uwe Marx,Volker Sandig
Publisher : John Wiley & Sons
Page : 318 pages
File Size : 53,7 Mb
Release : 2007-02-27
Category : Science
ISBN : 9783527609567

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Drug Testing In Vitro by Uwe Marx,Volker Sandig Pdf

Here, expert scientists from industry and academia share their knowledge on the assembly of functional human tissues in vitro and how to design drug screenings predictive of human exposure. In so doing, they combine the latest technological developments with strategic outlooks, such as novel cell and tissue systems for drug screening and testing, as well as emerging in vitro culture technologies. Equally importantly, the book does not shy away from regulatory acceptance and ethical issues.

Author : G. Stacey,Alan Doyle,Margherita Ferro
Publisher : Springer Science & Business Media
Page : 153 pages
File Size : 45,5 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9789401709965

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Cell Culture Methods for In Vitro Toxicology by G. Stacey,Alan Doyle,Margherita Ferro Pdf

Cell Culture Methods for in vitro Toxicology introduces the reader to a range of techniques involved in the use of in vitro cell culture in toxicological studies. It deals with major cell types studied in the field of toxicology and will be useful for anyone wishing to start work with animal cell cultures or to refresh their knowledge relating to in vitro cell models. Fundamental chapters deal with the general biology of cytotoxicity and cell immortalisation these are key issues for in vitro systems addressing the `3Rs' principle. Up-to-date overviews deal with the use of cells from liver, brain and intestine. In addition, biochemical analysis of cell responses, biotransformation pathways in cells and recombinant approaches to the early detection of cell stress are also covered in detail. Prominent features of in vitro technologies also include regulation, biosafety and standardisation. Dedicated chapters deal with these issues in a practical way in order to lead the reader to the right source of information. This book provides an up-to-date, informative and practical review of cell culture methods for in vitro toxicology. It will be of equal benefit to students and experienced toxicologists with little experience of in vitro cell culture.

Author : Bruno Sarmento
Publisher : Woodhead Publishing
Page : 408 pages
File Size : 50,9 Mb
Release : 2015-09-30
Category : Science
ISBN : 9780081001141

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Concepts and Models for Drug Permeability Studies by Bruno Sarmento Pdf

This book intends to be an updated compilation of the most important buccal, gastric, intestinal, pulmonary, nasal, vaginal, ocular, skin and blood-brain barrier in vitro models for predicting the permeability of drugs. Concepts and Models for Drug Permeability Studies focuses on different approaches and comprises of various models. Each model describes the protocol of seeding and conservation, the application for specific drugs, and takes into account the maintenance of physiologic characteristics and functionality of epithelium, from the simplest immortalized cell-based monoculture to the most complex engineered-tissue models. Chapters also discuss the equivalence between in vitro cell and tissue models and in vivo conditions, highlighting how each model may provisionally resemble a different drug absorption route. Updated information regarding the most recent in vitro models to study the permeability of drugs Short and concise chapters covering all the biological barriers with interest in drug permeability A combination of bibliographic information related with individual models and footnote instructions of technical procedures for construction of cell and tissue-based models Simple and clear scientific content, adaptable for young scientists and experimented researchers

Author : National Research Council,Institute of Medicine,Board on Life Sciences,Food and Nutrition Board,Committee on the Framework for Evaluating the Safety of the Dietary Supplements
Publisher : National Academies Press
Page : 527 pages
File Size : 42,8 Mb
Release : 2005-01-03
Category : Medical
ISBN : 9780309091107

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Dietary Supplements by National Research Council,Institute of Medicine,Board on Life Sciences,Food and Nutrition Board,Committee on the Framework for Evaluating the Safety of the Dietary Supplements Pdf

The growing consumer interest in health and fitness has expanded the market for a wide range of products, from yoga mats to the multiple dietary supplements now on the market. Supplements are popular, but are they safe? Many dietary supplements are probably safe when used as recommended. However, since 1994 when Congress decided that they should be regulated as if they were foods, they are assumed to be safe unless the Food and Drug Administration can demonstrate that they pose a significant risk to the consumer. But there are many types of products that qualify as dietary supplements, and the distinctions can become muddled and vague. Manufacturers are not legally required to provide specific information about safety before marketing their products. And the sales of supplements have been steadily increasingâ€"all together, the various types now bring in almost $16 billion per year. Given these confounding factors, what kind of information can the Food and Drug Administration use to effectively regulate dietary supplements? This book provides a framework for evaluating dietary supplement safety and protecting the health of consumers.

Author : Claus-Michael Lehr
Publisher : Unknown
Page : 0 pages
File Size : 52,7 Mb
Release : 2002
Category : Cell culture
ISBN : 0203275039

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Cell Culture Models of Biological Barriers by Claus-Michael Lehr Pdf

Author : S. Dawids
Publisher : Springer Science & Business Media
Page : 684 pages
File Size : 42,6 Mb
Release : 2012-12-06
Category : Technology & Engineering
ISBN : 9789401116404

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Test Procedures for the Blood Compatibility of Biomaterials by S. Dawids Pdf

This book represents the first European effort to provide a collection of test descriptions used in evaluation of the compatibility of biomaterials in contact with tissues and blood. The urge to compile this book arose from the fact that it is the properties of the material which ultimatively seem to determine the functional outcome of a medical device, almost regardless of how ingenious the construction of the very device is. The longer the exposure is, the more important these basic properties become. Unfortunately only a small part of the interactive phenomena is fully elucidated and understood. This challenge reflects itself in an effort to cover numerous aspects of testing, beginning with fundamental analysis of the material, continuing with the mechanical properties, the resistance to degradation and the analysis of surface and chemical properties including adsorption patterns of proteins ending with test on cell cultures, ex vivo and in vivo. A number of the tests which are generally accepted as being important are already described as official requirements (primarily Pharmacopeas). These official requirements are not included in order to limit the size of the book. It is the aim of this book to present the tests like a recipe in a uniform way to ease the reader in finding his/her way through the material and to present it as a kind of "cook-book" in an order to provide an easy access to copy the procedures. This has unfortunately not been possible in all circumstances.

Author : Radwa Ali Mehanna
Publisher : BoD – Books on Demand
Page : 294 pages
File Size : 41,7 Mb
Release : 2019-01-23
Category : Science
ISBN : 9781789848663

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Cell Culture by Radwa Ali Mehanna Pdf

Cell culture is one of the major tools used in cellular and molecular biology, delivering an excellent model for studying the normal physiology and biochemistry of cells. This book covers some advanced aspects in cell culture methodologies. The book has four sections discussing different types of cell culture models, including 3D cell culture techniques, their advantages, and limitations in comparison to traditional 2D culturing; cell viability, autophagy, in vitro toxicity tests and live cell imaging; stem cell culture for cell-based therapeutics; and specific applications and methodologies for hybrid cell lines and cancer models. This book provides a comprehensive overview of some of the advanced cell culture methodologies and applications. It serves as a valuable source for scientists, researchers, clinicians and students.

Author : Frank A. Barile
Publisher : CRC Press
Page : 301 pages
File Size : 53,8 Mb
Release : 2019-06-04
Category : Medical
ISBN : 9781000012910

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Introduction to In Vitro Cytotoxicology by Frank A. Barile Pdf

First Published in 1994, Introduction to In Vitro Cytotoxicology examines in vitro cytotoxicology, which offers new methodologies to toxicity testing. This important new discipline of modern toxicology is gaining increased acceptance as a viable alternative to traditional testing methods. The text discusses the application of in vitro cytotoxicology to toxicity testing and human risk assessment, and it analyzes the advantages and limitations of the tests performed under scientific and regulatory conditions. The book also reviews the optimum utilization of certain tests for specific groups of chemicals relevant to validation programs currently in progress. This book is a useful reference tool for students, researchers, and practitioners interested in academic, industrial, and regulatory toxicology; environmental health; cell biology; pharmacology; dentistry; or human and veterinary medicine.

Author : OECD
Publisher : OECD Publishing
Page : 27 pages
File Size : 51,9 Mb
Release : 2016-07-29
Category : Electronic
ISBN : 9789264264885

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OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 489: In Vivo Mammalian Alkaline Comet Assay by OECD Pdf

The in vivo alkaline single cell gel electrophoresis assay, also called alkaline Comet Assay is a method measuring DNA strand breaks in eukaryotic cells.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 44,9 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Kitty Verhoeckx,Paul Cotter,Iván López-Expósito,Charlotte Kleiveland,Tor Lea,Alan Mackie,Teresa Requena,Dominika Swiatecka,Harry Wichers
Publisher : Springer
Page : 338 pages
File Size : 52,8 Mb
Release : 2015-04-29
Category : Technology & Engineering
ISBN : 9783319161044

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The Impact of Food Bioactives on Health by Kitty Verhoeckx,Paul Cotter,Iván López-Expósito,Charlotte Kleiveland,Tor Lea,Alan Mackie,Teresa Requena,Dominika Swiatecka,Harry Wichers Pdf

“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.