Cilia From Mechanisms To Disease Part A

Author : Anonim
Publisher : Elsevier
Page : 270 pages
File Size : 43,6 Mb
Release : 2023-03-24
Category : Science
ISBN : 9780443185878

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Cilia: From Mechanisms to Disease–Part A by Anonim Pdf

Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more. Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more. Offers a detailed overview of the protocols used to study cilia structure and various aspects of ciliary function Provides an approach to the study of some diseases related to ciliary dysfunction, also known as ciliopathies Written in an accessible style by renowned experts in the field

Author : Anonim
Publisher : Elsevier
Page : 268 pages
File Size : 41,9 Mb
Release : 2023-04
Category : Science
ISBN : 9780443185861

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Cilia: From Mechanisms to Disease-Part A by Anonim Pdf

Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more. Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.

Author : Anonim
Publisher : Elsevier
Page : 270 pages
File Size : 41,5 Mb
Release : 2023-05
Category : Science
ISBN : 9780443185885

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Cilia: From Mechanisms to Disease-Part B by Anonim Pdf

Cilia: From Mechanisms to Disease Part B, Volume 176 of the Methods in Cell Biology series, presents interesting chapters on topics such as The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish, Human LUHMES and NES cells as models for studying primary cilia in neurons, Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations, Single-molecule imaging in the primary cilium, Methods to analyze primary cilia in mouse cardiac lesion model, Methods to study motile ciliated cell types in a zebrafish brain mode, and more. Other sections cover Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome), Modeling ciliopathies in patient-derived primary cells, Analysis of motility and mucociliary function of tracheal epithelial cilia, High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium, Methods for siRNA delivery in retina explants, Methods to study primary cilia and autophagy in the brain, and more.

Author : Anonim
Publisher : Academic Press
Page : 0 pages
File Size : 47,9 Mb
Release : 2023-04-21
Category : Science
ISBN : 0443185883

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Cilia: From Mechanisms to Disease-Part B by Anonim Pdf

Cilia: From Mechanisms to Disease Part B, Volume 176 of the Methods in Cell Biology series, presents interesting chapters on topics such as The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish, Human LUHMES and NES cells as models for studying primary cilia in neurons, Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations, Single-molecule imaging in the primary cilium, Methods to analyze primary cilia in mouse cardiac lesion model, Methods to study motile ciliated cell types in a zebrafish brain mode, and more. Other sections cover Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome), Modeling ciliopathies in patient-derived primary cells, Analysis of motility and mucociliary function of tracheal epithelial cilia, High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium, Methods for siRNA delivery in retina explants, Methods to study primary cilia and autophagy in the brain, and more.

Author : Anonim
Publisher : Academic Press
Page : 408 pages
File Size : 43,9 Mb
Release : 2009-11-30
Category : Science
ISBN : 0080962823

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Primary Cilia by Anonim Pdf

In recent years, the role of cilia in the study of health, development and disease has been increasingly clear, and new discoveries have made this an exciting and important field of research. This comprehensive volume, a complement to the new three-volume treatment of cilia and flagella by King and Pazour, presents easy-to-follow protocols and detailed background information for researchers working with cilia and flagella. *Covers protocols for primary cilia across several systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians and scientists working in a wide range of fields

Author : Douglas W Houston
Publisher : Academic Press
Page : 236 pages
File Size : 54,7 Mb
Release : 2017-11-14
Category : Science
ISBN : 9780128125915

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Cell Polarity in Development and Disease by Douglas W Houston Pdf

Cell Polarity in Development and Disease offers insights into the basic molecular mechanisms of common diseases that arise as a result of a loss of ordered organization and intrinsic polarity. Included are diseases affecting highly polarized epithelial tissues in the lung and kidney, as well as loss and gain of cell polarity in the onset and progression of cancer. This book provides a basic resource for understanding the biology of polarity, offering a starting point for those thinking of targeting cell polarity for translational medical research. Provides basic science understanding of cell polarity disease and development Covers diseases affecting polarized epithelial tissues in the lung and kidney, also covering the progression of cancer Includes historical context of cell polarity research for potential future breakthroughs

Author : Jong Hoon Park,Curie Ahn
Publisher : Springer
Page : 126 pages
File Size : 55,9 Mb
Release : 2016-10-12
Category : Medical
ISBN : 9789811020414

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Cystogenesis by Jong Hoon Park,Curie Ahn Pdf

Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a highly prevalent hereditary renal disorder in which fluid-filled cysts are appeared in both kidneys. Main causative genes of ADPKD are PKD1 and PKD2, encoding for polycystin-1 (PC1) and polycystin-2 (PC2) respectively. Those proteins are localized on primary cilia and function as mechanosensor in response to the fluid flow, translating mechanistic stimuli into calcium signaling. With mutations either of PKD1 or PKD2, hyper-activated renal tubular epithelial cell proliferation is observed, followed by disrupted calcium homeostasis and aberrant intracellular cyclic AMP (cAMP) accumulation. Increased cell proliferation with fluid secretion leads to the development of thousands of epithelial-lined, fluid-filled cysts in kidneys. It is also accompanied by interstitial inflammation, fibrosis, and finally reaching end-stage renal disease (ESRD). In human ADPKD, the age at which renal failure typically occurs is later in life, however no specific targeted medications are available to cure ADPKD. Recently, potential therapeutic targets or surrogate diagnostic biomarkers for ADPKD are proposed with the advances in the understanding of ADPKD pathogenesis, and some of them were attempted for clinical trials. Herein, we will summarize genetic and epi-genetic molecular mechanisms in ADPKD progression, and overview the currently available biomarkers or potential therapeutic reagents suggested.

Author : Lorenzo Galluzzi,Sheila Spada
Publisher : Academic Press
Page : 244 pages
File Size : 50,6 Mb
Release : 2022-02-11
Category : Science
ISBN : 9780323855020

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MCB: CAR T Cells: Development, Characterization and Applications by Lorenzo Galluzzi,Sheila Spada Pdf

MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using ?-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells

Author : Anonim
Publisher : Academic Press
Page : 228 pages
File Size : 44,7 Mb
Release : 2022-07-08
Category : Science
ISBN : 9780323900218

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Methods in Stem Cell Biology - Part A by Anonim Pdf

Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of Methods in Cancer Stem Cell Biology

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 40,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Anonim
Publisher : Academic Press
Page : 262 pages
File Size : 42,6 Mb
Release : 2022-08-09
Category : Science
ISBN : 9780323900195

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Methods in Stem Cell Biology - Part B by Anonim Pdf

Methods in Cancer Stem Cell Biology: Part B, Volume 171 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of Methods in Cancer Stem Cell Biology

Author : Anonim
Publisher : Academic Press
Page : 603 pages
File Size : 50,9 Mb
Release : 1995-08-31
Category : Science
ISBN : 0080859453

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Cilia and Flagella by Anonim Pdf

Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella. Key Features * A complete guide containing more than 80 concise technical chapters friendly to both the novice and experienced researcher * Covers protocols for cilia and flagella across systems and species from Chlamydomonas and Euglena to mammals * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time, including microscopy, electrophoresis, and PCR * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

Author : Wallace F. Marshall,Renata Basto
Publisher : Unknown
Page : 0 pages
File Size : 41,7 Mb
Release : 2016-11-30
Category : Science
ISBN : 1621821412

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Cilia by Wallace F. Marshall,Renata Basto Pdf

Nearly every cell in the human body has one or more protrusive structures called cilia or flagella. These power cell movement and fluid flow, sense the extracellular environment, coordinate cell signaling, and establish left-right asymmetry during development. Mutations in genes that encode cilia can lead to disorders known as ciliopathies. Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology examines key aspects of ciliary biology-from the molecular to the organismal level-in normal physiology and disease. The contributors dissect the complex structures of motile and nonmotile (primary) cilia, discuss how the intraflagellar transport machinery moves cargo across the central axoneme, and review how the ciliary gate controls the composition of cilia and flagella. The roles of cilia in coordinating cellular responses to environmental stimuli via cell signaling pathways (e.g., Hedgehog) are also covered, as are physiological functions in processes such as fertilization, mucociliary clearance, and vision. The authors also survey the wide spectrum of ciliopathies, describing their genetic bases, pathogenic mechanisms, and clinical manifestations. This volume is therefore an indispensable reference for all cell and developmental biologists, as well as medical geneticists and clinical scientists wishing to understand and treat disorders involving ciliary dysfunction.

Author : Anonim
Publisher : Academic Press
Page : 2390 pages
File Size : 51,8 Mb
Release : 2020-06-26
Category : Science
ISBN : 9780128140826

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Encyclopedia of Bone Biology by Anonim Pdf

Encyclopedia of Bone Biology, Three Volume Set covers hot topics from within the rapidly expanding field of bone biology and skeletal research, enabling a complete understanding of both bone physiology and its relation to other organs and pathophysiology. This encyclopedia will serve as a vital resource for those involved in bone research, research in other fields that cross link with bone, such as metabolism and immunology, and physicians who treat bone diseases. Each article provides a comprehensive overview of the selected topic to inform a broad spectrum of readers from advanced undergraduate students to research professionals. Chapters also explore the latest advances and hot topics that have emerged in recent years, including the Hematopoietic Niche and Nuclear Receptors. In the electronic edition, each chapter will include hyperlinked references and further readings as well as cross-references to related articles. Incorporates perspectives from experts working within the domains of biomedicine, including physiology, pathobiology, pharmacology, immunology, endocrinology, orthopedics and metabolism Provides an authoritative introduction for non-specialists and readers from undergraduate level upwards, as well as up-to-date foundational content for those familiar with the field Includes multimedia features, cross-references and color images/videos

Author : Takami Yamaguchi,Takuji Ishikawa,Yohsuke Imai
Publisher : William Andrew
Page : 314 pages
File Size : 46,9 Mb
Release : 2018-06-27
Category : Medical
ISBN : 9780323389594

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Integrated Nano-Biomechanics by Takami Yamaguchi,Takuji Ishikawa,Yohsuke Imai Pdf

Integrated Nano-Biomechanics provides an integrated look into the rapidly evolving field of nanobiomechanics. The book demystifies the processes in living organisms at the micro- and nano-scale through mechanics, using theoretical, computational and experimental means. The book develops the concept of integrating different technologies along the hierarchical structure of biological systems and clarifies biomechanical interactions among different levels for the analysis of multi-scale pathophysiological phenomena. With a focus on nano-scale processes and biomedical applications, it is shown how knowledge obtained can be utilized in a range of areas, including diagnosis and treatment of various human diseases and alternative energy production. This book is based on collaboration of researchers from a unique combination of fields, including biomechanics, computational mechanics, GPU application, electron microscopy, biology of motile micro-organisms, entomological mechanics and clinical medicine. The book will be of great interest to scientists and researchers involved in disciplines, such as micro- and nano-engineering, bionanotechnology, biomedical engineering, micro- and nano-scale fluid-mechanics (such as in MEMS devices), nanomedicine and microbiology, as well as industries such as optical devices, computer simulation, plant based energy sources and clinical diagnosis of the gastric diseases. Provides knowledge of integrated biomechanics, focusing on nano-scale, in this rapidly growing research field Explains how the different technologies can be integrated and applied in a variety of biomedical application fields, as well as for alternative energy sources Uses a collaborative, multidisciplinary approach to provide a comprehensive coverage of nano-biomechanics