Confocal Microscopy

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Handbook of Biological Confocal Microscopy

James Pawley
Handbook of Biological Confocal Microscopy Book PDF

Author : James Pawley
Publisher : Springer Science & Business Media
Page : 639 pages
File Size : 47,8 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475753486

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Handbook of Biological Confocal Microscopy by James Pawley Pdf

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Basic Confocal Microscopy

W. Gray (Jay) Jerome,Robert L. Price
Basic Confocal Microscopy Book PDF

Author : W. Gray (Jay) Jerome,Robert L. Price
Publisher : Springer
Page : 368 pages
File Size : 40,7 Mb
Release : 2018-10-30
Category : Science
ISBN : 9783319974545

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Basic Confocal Microscopy by W. Gray (Jay) Jerome,Robert L. Price Pdf

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.

Confocal Microscopy for Biologists

Alan R. Hibbs
Confocal Microscopy for Biologists Book PDF

Author : Alan R. Hibbs
Publisher : Springer Science & Business Media
Page : 467 pages
File Size : 51,6 Mb
Release : 2004-04-30
Category : Science
ISBN : 9780306485657

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Confocal Microscopy for Biologists by Alan R. Hibbs Pdf

There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.

Confocal Microscopy

Jian Liu,Jiubin Tan
Confocal Microscopy Book PDF

Author : Jian Liu,Jiubin Tan
Publisher : Morgan & Claypool Publishers
Page : 108 pages
File Size : 53,6 Mb
Release : 2016-12-21
Category : Science
ISBN : 9781681743387

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Confocal Microscopy by Jian Liu,Jiubin Tan Pdf

The confocal microscope is appropriate for imaging cells or the measurement of industrial artefacts. However, junior researchers and instrument users sometimes misuse imaging concepts and metrological characteristics, such as position resolution in industrial metrology and scale resolution in bio-imaging. And, metrological characteristics or influence factors in 3D measurement such as height assessment error caused by 3D coupling effect are so far not yet identified. In this book, the authors outline their practices by the working experiences on standardization and system design. This book assumes little previous knowledge of optics, but rich experience in engineering of industrial measurements, in particular with profile metrology or areal surface topography will be very helpful to understand the theoretical concerns and value of the technological advances. It should be useful for graduate students or researchers as extended reading material, as well as microscope users alongside their handbook.

Confocal Microscopy

Tony Wilson
Confocal Microscopy Book PDF

Author : Tony Wilson
Publisher : Unknown
Page : 456 pages
File Size : 53,8 Mb
Release : 1990
Category : Confocal microscopy
ISBN : UOM:39015031800868

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Confocal Microscopy by Tony Wilson Pdf

Numerous applications of confocal microscopes include the life sciences, ophthamology, industrial inspection and semiconductor linewidth metrology. Concentrating on the science and applications of confocal microscopy, this book includes all the latest developments in three-dimensional processing techniques.

Confocal Microscopy

Stephen W. Paddock
Confocal Microscopy Book PDF

Author : Stephen W. Paddock
Publisher : Springer Science & Business Media
Page : 446 pages
File Size : 52,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597222

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Confocal Microscopy by Stephen W. Paddock Pdf

In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.

Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology

R.F. Guthoff,C. Baudouin,J. Stave
Atlas of Confocal Laser Scanning In vivo Microscopy in Ophthalmology Book PDF

Author : R.F. Guthoff,C. Baudouin,J. Stave
Publisher : Springer Science & Business Media
Page : 207 pages
File Size : 48,9 Mb
Release : 2007-05-16
Category : Medical
ISBN : 9783540327073

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Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology by R.F. Guthoff,C. Baudouin,J. Stave Pdf

This lavishly illustrated atlas demonstrates normal in-vivo anatomy of the cornea, limbus, and conjunctiva; quantifies various cellular structures using cell-density calculations; and establishes correlations between novel optical sections of various diseases of the ocular surface and clinical findings. It also describes early signs of different eye diseases and supports the reader in diagnostic and therapeutic management.

Reflectance Confocal Microscopy for Skin Diseases

Rainer Hofmann-Wellenhof,Giovanni Pellacani,Joseph Malvehy,H. Peter Soyer
Reflectance Confocal Microscopy for Skin Diseases Book PDF

Author : Rainer Hofmann-Wellenhof,Giovanni Pellacani,Joseph Malvehy,H. Peter Soyer
Publisher : Springer Science & Business Media
Page : 484 pages
File Size : 55,9 Mb
Release : 2012-02-07
Category : Medical
ISBN : 9783642219979

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Reflectance Confocal Microscopy for Skin Diseases by Rainer Hofmann-Wellenhof,Giovanni Pellacani,Joseph Malvehy,H. Peter Soyer Pdf

This book focuses on the use and significance of in vivo reflectance confocal microscopy (RCM) for non-invasive high-resolution imaging of the skin. All of the chapters in this hands-on guide are generously illustrated with numerous confocal images and structured in a reader-friendly way. The contents include detailed information on the most relevant and up-to-date aspects of RCM, schematic drawings summarizing and explaining the most important RCM criteria, and a chapter specifically devoted to bridging the gap between dermoscopy, RCM, and histopathology. At the end of each chapter, core messages recapitulate the most pertinent aspects. Reflectance Confocal Microscopy for Skin Diseases will be a valuable resource for all physicians involved in the diagnosis and treatment of neoplastic and inflammatory skin diseases.

Introduction to Confocal Fluorescence Microscopy

Michiel Muller
Introduction to Confocal Fluorescence Microscopy Book PDF

Author : Michiel Muller
Publisher : SPIE Press
Page : 142 pages
File Size : 55,6 Mb
Release : 2006
Category : Confocal fluorescence microscopy
ISBN : 0819460435

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Introduction to Confocal Fluorescence Microscopy by Michiel Muller Pdf

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Confocal Scanning Optical Microscopy and Related Imaging Systems

Gordon S. Kino,Timothy R. Corle
Confocal Scanning Optical Microscopy and Related Imaging Systems Book PDF

Author : Gordon S. Kino,Timothy R. Corle
Publisher : Academic Press
Page : 335 pages
File Size : 52,7 Mb
Release : 1996-09-18
Category : Technology & Engineering
ISBN : 008052978X

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Confocal Scanning Optical Microscopy and Related Imaging Systems by Gordon S. Kino,Timothy R. Corle Pdf

This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications Discusses the theory and design of near-field optical microscopes Explains phase imaging in the scanning optical and interference microscopes

Confocal Raman Microscopy

Jan Toporski,Thomas Dieing,Olaf Hollricher
Confocal Raman Microscopy Book PDF

Author : Jan Toporski,Thomas Dieing,Olaf Hollricher
Publisher : Springer
Page : 596 pages
File Size : 45,6 Mb
Release : 2018-03-01
Category : Science
ISBN : 9783319753805

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Confocal Raman Microscopy by Jan Toporski,Thomas Dieing,Olaf Hollricher Pdf

This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.

Reflectance Confocal Microscopy of Cutaneous Tumors

Salvador Gonzalez
Reflectance Confocal Microscopy of Cutaneous Tumors Book PDF

Author : Salvador Gonzalez
Publisher : CRC Press
Page : 849 pages
File Size : 52,8 Mb
Release : 2017-07-06
Category : Medical
ISBN : 9781498757621

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Reflectance Confocal Microscopy of Cutaneous Tumors by Salvador Gonzalez Pdf

Reflectance confocal microscopy enables lesions in skin to be examined without excision, but with improved diagnostic accuracy, assessment of dermoscopic-histologic correlation, assessment of surgical margins, as well as speed and convenience for the physician and patient. This extensively enlarged and updated text reviews the current and future state of the art for those involved with the diagnosis and treatment of skin tumors, with a greatly increased amount of material on the expected normal patterns of skin throughout life and on non-melanocytic tumors.

Basic Confocal Microscopy

Robert L. Price,W. Gray (Jay) Jerome
Basic Confocal Microscopy Book PDF

Author : Robert L. Price,W. Gray (Jay) Jerome
Publisher : Springer Science & Business Media
Page : 302 pages
File Size : 42,7 Mb
Release : 2011-04-11
Category : Science
ISBN : 9780387781754

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Basic Confocal Microscopy by Robert L. Price,W. Gray (Jay) Jerome Pdf

Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.

Handbook of Biological Confocal Microscopy

James Pawley
Handbook of Biological Confocal Microscopy Book PDF

Author : James Pawley
Publisher : Springer Science & Business Media
Page : 1014 pages
File Size : 55,7 Mb
Release : 2010-08-04
Category : Science
ISBN : 9780387455242

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Handbook of Biological Confocal Microscopy by James Pawley Pdf

Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.

Confocal Microscopy

Andrea White
Confocal Microscopy Book PDF

Author : Andrea White
Publisher : Unknown
Page : 0 pages
File Size : 46,8 Mb
Release : 2017
Category : Confocal microscopy
ISBN : 1536106275

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Confocal Microscopy by Andrea White Pdf

Confocal laser microscopy is a microscopic observation technique patented by Minsk in 1957. Its success is due to the undeniable advantages over traditional optical microscopy including improved image clarity and contrast, greater vertical and horizontal resolution, etc., and above all the possibility to take optical sections of the sample, which allows us to obtain a tridimensional study. This authors of this book provide and discuss new research on the principles, techniques and applications of confocal microscopy.