Handbook Of Biological Confocal Microscopy

Author : James Pawley
Publisher : Springer Science & Business Media
Page : 639 pages
File Size : 55,7 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475753486

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Handbook of Biological Confocal Microscopy by James Pawley Pdf

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Author : James Pawley
Publisher : Springer Science & Business Media
Page : 234 pages
File Size : 55,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461571339

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Handbook of Biological Confocal Microscopy by James Pawley Pdf

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Author : James B. Pawley
Publisher : Springer Science & Business Media
Page : 672 pages
File Size : 49,5 Mb
Release : 1995
Category : Medical
ISBN : 0306448262

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Handbook of Biological Confocal Microscopy by James B. Pawley Pdf

This newly updated second edition details the latest instrumentation and applications of the confocal microscope. This edition features 21 new chapters and includes information on preparing living specimens for the confocal microscope.

Author : James B. Pawley
Publisher : Unknown
Page : 0 pages
File Size : 50,7 Mb
Release : 1989
Category : Confocal microscopy
ISBN : OCLC:20871222

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The Handbook of Biological Confocal Microscopy by James B. Pawley Pdf

Author : James Pawley
Publisher : Springer Science & Business Media
Page : 1014 pages
File Size : 48,6 Mb
Release : 2010-08-04
Category : Science
ISBN : 9780387455242

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Handbook of Biological Confocal Microscopy by James Pawley Pdf

Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.

Author : Anonim
Publisher : Academic Press
Page : 521 pages
File Size : 43,8 Mb
Release : 2003-01-03
Category : Science
ISBN : 9780080925356

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Cell Biological Applications of Confocal Microscopy by Anonim Pdf

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

Author : Robert L. Price,W. Gray (Jay) Jerome
Publisher : Springer Science & Business Media
Page : 302 pages
File Size : 40,7 Mb
Release : 2011-04-11
Category : Science
ISBN : 9780387781754

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Basic Confocal Microscopy by Robert L. Price,W. Gray (Jay) Jerome Pdf

Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.

Author : Gauri Misra
Publisher : Academic Press
Page : 188 pages
File Size : 48,6 Mb
Release : 2019-03-23
Category : Medical
ISBN : 9780128172803

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Data Processing Handbook for Complex Biological Data Sources by Gauri Misra Pdf

Data Processing Handbook for Complex Biological Data provides relevant and to the point content for those who need to understand the different types of biological data and the techniques to process and interpret them. The book includes feedback the editor received from students studying at both undergraduate and graduate levels, and from her peers. In order to succeed in data processing for biological data sources, it is necessary to master the type of data and general methods and tools for modern data processing. For instance, many labs follow the path of interdisciplinary studies and get their data validated by several methods. Researchers at those labs may not perform all the techniques themselves, but either in collaboration or through outsourcing, they make use of a range of them, because, in the absence of cross validation using different techniques, the chances for acceptance of an article for publication in high profile journals is weakened. Explains how to interpret enormous amounts of data generated using several experimental approaches in simple terms, thus relating biology and physics at the atomic level Presents sample data files and explains the usage of equations and web servers cited in research articles to extract useful information from their own biological data Discusses, in detail, raw data files, data processing strategies, and the web based sources relevant for data processing

Author : Barry R. Masters,Peter So
Publisher : Oxford University Press
Page : 896 pages
File Size : 55,5 Mb
Release : 2008-05-19
Category : Science
ISBN : 0198036825

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Handbook of Biomedical Nonlinear Optical Microscopy by Barry R. Masters,Peter So Pdf

The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Author : W. Gray (Jay) Jerome,Robert L. Price
Publisher : Springer
Page : 368 pages
File Size : 43,6 Mb
Release : 2018-10-30
Category : Science
ISBN : 9783319974545

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Basic Confocal Microscopy by W. Gray (Jay) Jerome,Robert L. Price Pdf

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 52,5 Mb
Release : 2017-03-27
Category : Medical
ISBN : 9783527687725

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Author : B.G. Yacobi,D.B. Holt,Lawrence L. Kazmerski
Publisher : Springer Science & Business Media
Page : 484 pages
File Size : 42,9 Mb
Release : 1994-02-28
Category : Science
ISBN : 030644433X

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Microanalysis of Solids by B.G. Yacobi,D.B. Holt,Lawrence L. Kazmerski Pdf

This book systematically describes the most widely used techniques for the microanalysis of the physical, structural, and compositional properties of solids. Covering electron beams, ion beams, photon beams, and acoustic waves, it will provide physicists, materials scientists, electrical engineers, chemists, and their students with a comprehensive reference source.

Author : Spencer L. Shorte,Friedrich Frischknecht
Publisher : Springer Science & Business Media
Page : 450 pages
File Size : 52,5 Mb
Release : 2007-09-12
Category : Science
ISBN : 9783540713319

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Imaging Cellular and Molecular Biological Functions by Spencer L. Shorte,Friedrich Frischknecht Pdf

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.

Author : Shinya Inoue
Publisher : Springer Science & Business Media
Page : 600 pages
File Size : 40,5 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781475769258

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Video Microscopy by Shinya Inoue Pdf

Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Author : Anonim
Publisher : Elsevier
Page : 507 pages
File Size : 48,8 Mb
Release : 2003-01-04
Category : Science
ISBN : 9780080496580

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Cell Biological Applications of Confocal Microscopy by Anonim Pdf

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures