Cytogenomics

Author : Thomas Liehr
Publisher : Academic Press
Page : 430 pages
File Size : 40,5 Mb
Release : 2021-05-25
Category : Science
ISBN : 9780128235805

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Cytogenomics by Thomas Liehr Pdf

Cytogenomics demonstrates that chromosomes are crucial in understanding the human genome and that new high-throughput approaches are central to advancing cytogenetics in the 21st century. After an introduction to (molecular) cytogenetics, being the basic of all cytogenomic research, this book highlights the strengths and newfound advantages of cytogenomic research methods and technologies, enabling researchers to jump-start their own projects and more effectively gather and interpret chromosomal data. Methods discussed include banding and molecular cytogenetics, molecular combing, molecular karyotyping, next-generation sequencing, epigenetic study approaches, optical mapping/karyomapping, and CRISPR-cas9 applications for cytogenomics. The book’s second half demonstrates recent applications of cytogenomic techniques, such as characterizing 3D chromosome structure across different tissue types and insights into multilayer organization of chromosomes, role of repetitive elements and noncoding RNAs in human genome, studies in topologically associated domains, interchromosomal interactions, and chromoanagenesis. This book is an important reference source for researchers, students, basic and translational scientists, and clinicians in the areas of human genetics, genomics, reproductive medicine, gynecology, obstetrics, internal medicine, oncology, bioinformatics, medical genetics, and prenatal testing, as well as genetic counselors, clinical laboratory geneticists, bioethicists, and fertility specialists. Offers applied approaches empowering a new generation of cytogenomic research using a balanced combination of classical and advanced technologies Provides a framework for interpreting chromosome structure and how this affects the functioning of the genome in health and disease Features chapter contributions from international leaders in the field

Author : Tony Heitkam,Sònia Garcia
Publisher : Springer Nature
Page : 563 pages
File Size : 43,7 Mb
Release : 2023-07-21
Category : Science
ISBN : 9781071632260

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Plant Cytogenetics and Cytogenomics by Tony Heitkam,Sònia Garcia Pdf

This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.

Author : Ricardo Utsunomia,Francisco J. Ruiz-Ruano,Magdalena Vaio
Publisher : Frontiers Media SA
Page : 157 pages
File Size : 55,7 Mb
Release : 2022-07-04
Category : Science
ISBN : 9782889764914

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Cytogenomics: Structural Organization and Evolution of Genomes by Ricardo Utsunomia,Francisco J. Ruiz-Ruano,Magdalena Vaio Pdf

Author : Marilyn S. Arsham,Margaret J. Barch,Helen J. Lawce
Publisher : John Wiley & Sons
Page : 1216 pages
File Size : 44,7 Mb
Release : 2017-04-24
Category : Science
ISBN : 9781119061229

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The AGT Cytogenetics Laboratory Manual by Marilyn S. Arsham,Margaret J. Barch,Helen J. Lawce Pdf

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.

Author : Jean McGowan-Jordan,Ros J. Hastings,Sarah Moore
Publisher : Unknown
Page : 164 pages
File Size : 40,6 Mb
Release : 2020-12-31
Category : Electronic
ISBN : 3318067067

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Iscn 2020 by Jean McGowan-Jordan,Ros J. Hastings,Sarah Moore Pdf

This reprint of 'Cytogenetic and Genome Research' contains contributions discussing the subject in-depth. 'Cytogenetic and Genome Research' is a well-respected, international peer-reviewed journal in genetics.

Author : Thomas Liehr
Publisher : CRC Press
Page : 422 pages
File Size : 49,6 Mb
Release : 2022-12-07
Category : Science
ISBN : 9781000800401

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Cytogenetics and Molecular Cytogenetics by Thomas Liehr Pdf

Genomic technologies provide the means of diagnosis and management of many human diseases. Without insights from cytogenetics, correct interpretation of modern high-throughput results is difficult, if not impossible. This book summarizes applications of cytogenetics and molecular cytogenetics for students, clinicians and researchers in genetics, genomics and diagnostics. The book combines the state-of-the-art knowledge and practical expertise from leading researchers and clinicians and provides a comprehensive overview of current medical and research applications of many of these technologies. KEY FEATURES • Provides clear summaries of fluorescence in situ hybridization technologies and others • Comprehensively covers established and emerging methods • Chapters from an international team of leading researchers • Useful for students, researchers and clinicians

Author : Steven Gersen,Martha B. Keagle
Publisher : Humana Press
Page : 0 pages
File Size : 49,6 Mb
Release : 2010-10-12
Category : Medical
ISBN : 1617370703

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The Principles of Clinical Cytogenetics by Steven Gersen,Martha B. Keagle Pdf

This book provides a comprehensive, in-depth explanation of the basic concepts and interpretations involved in chromosome analysis, a critical technique in the diagnosis, prognosis, and monitoring of a wide variety of conditions. Designed for the health care provider who must use and explain the often complex results of these tests, this book details in understandable language the various applications of chromosome analysis in clinical settings and the clinical significance of abnormal results. In addition, the book offers an informative tutorial on basic laboratory procedures (including microscopy, photomicrography, automation, computerized karyotyping, and QA/QC), reports on novel synergistic technologies such as FISH, and discusses issues in genetic counseling. Enlightening and accessible, The Principles of Clinical Cytogenetics constitutes an indispensable reference for today's physicians and managed care practitioners who depend on the cytogenetics laboratory for the diagnosis of their patients' ailments.

Author : Marcelo L. Larramendy,Sonia Soloneski
Publisher : BoD – Books on Demand
Page : 174 pages
File Size : 51,7 Mb
Release : 2019-05-10
Category : Medical
ISBN : 9781838804053

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Cytogenetics by Marcelo L. Larramendy,Sonia Soloneski Pdf

Cytogenetics - Past, Present, and Further Perspectives discusses events that influenced the development of cytogenetics as a specialty within biology, with special attention paid to methodological achievements developed worldwide that have driven the field forward. Improvements to the resolution of chromosome analysis followed closely the introduction of innovative analytical technologies. In that sense, this book reviews and provides a brief account of the structure of chromosomes and stresses the high structural conservation in different species with an emphasis on aspects that require further research. However, it should be kept in mind that the future of cytogenetics will likely depend on improved knowledge of chromosome structure and function.

Author : Sverre Heim,Felix Mitelman
Publisher : John Wiley & Sons
Page : 648 pages
File Size : 54,5 Mb
Release : 2015-06-29
Category : Medical
ISBN : 9781118795514

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Cancer Cytogenetics by Sverre Heim,Felix Mitelman Pdf

The first three editions of this acclaimed book presented a much-needed conceptual synthesis of this rapidly moving field. Now, Cancer Cytogenetics, Fourth Edition, offers a comprehensive, expanded, and up-to-date review of recent dramatic advances in this area, incorporating a vast amount of new data from the latest basic and clinical investigations. New contributors reflecting broader international authorship and even greater expertise Greater emphasis throughout on the clinical importance and application of information about cytogenetic and molecular aberrations Includes a complete coverage of chromosome aberrations in cancer based on an assessment of the 60,000 neoplasms cytogenetically investigated to date Now produced in full color for enhanced clarity Covers how molecular genetic data (PCR-based and sequencing information) are collated with the cytogenetic data where pertinent Discusses how molecular cytogenetic data (based on studies using FISH, CGH, SNP, etc) are fused with karyotyping data to enable an as comprehensive understanding of cancer cytogenetics as is currently possible

Author : Steven L. Gersen,Martha B. Keagle
Publisher : Springer Science & Business Media
Page : 589 pages
File Size : 40,7 Mb
Release : 2008-08-17
Category : Medical
ISBN : 9781592598335

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The Principles of Clinical Cytogenetics by Steven L. Gersen,Martha B. Keagle Pdf

In the summer of 1989, one of us (SLG), along with his mentor, Dorothy Warb- ton, attended the Tenth International Workshop on Human Gene Mapping. The me- ing was held at Yale University in celebration of the first such event, which also took place there. This meeting was not open to the general public; one had to have contributed to mapping a gene to be permitted to attend. The posters, of course, were therefore all related to gene mapping, and many were covered with pretty, colorful pictures of a novel, fluorescent application of an old technology, in situ hybridization. Walking through the room, Dorothy remarked that, because of this new FISH technique, ch- mosomes, which had become yesterday’s news, were once again “back in style. ” Approximately three years later, a commercial genetics company launched a FISH assay for prenatal ploidy detection. A substantial number of cytogeneticists across the country reacted with a combination of outrage and panic. Many were concerned that physicians would be quick to adopt this newfangled upstart test and put us all on the unemployment line. They did not at the time realize what Dorothy instinctively already knew—that FISH would not spell the doom of the cytogenetics laboratory, but it would, rather, take it to new heights.

Author : Usha Dutta
Publisher : Cambridge Scholars Publishing
Page : 265 pages
File Size : 50,5 Mb
Release : 2022-03-22
Category : Science
ISBN : 9781527580657

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Essentials of Cytogenetic and Molecular Cytogenetic Laboratory Testing by Usha Dutta Pdf

This book is a compilation of cytogenetic and molecular cytogenetic techniques that are routinely performed in a molecular cytogenetic laboratory. It provides a summary of chromosomal disorders and mechanisms, along with pictures and details of laboratory procedures. Due to the simplicity of the language used, the principles and techniques discussed here are easily understandable. The book also details modern techniques, which will be of interest for geneticists, academicians, scientists, and clinical geneticists aspiring to establish a molecular cytogenetic lab. It also serves to help geneticists understand each protocol as it is written in a self-explanatory manner for standardizing techniques in their laboratory.

Author : International Standing Committee on Human Cytogenetic Nomenclature
Publisher : Karger Medical and Scientific Publishers
Page : 148 pages
File Size : 43,7 Mb
Release : 2013
Category : Medical
ISBN : 9783318022537

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ISCN 2013 by International Standing Committee on Human Cytogenetic Nomenclature Pdf

This publication extends the now classic system of human cytogenetic nomenclature prepared by an expert committee and published in collaboration with Cytogenetic and Genome Research' since 1963. Revised and finalized by the ISCN Committee and its advisors at a meeting in Seattle, Wash., in April 2012, the ISCN 2013 updates, revises and incorporates all previous human cytogenetic nomenclature recommendations into one systematically organized publication that supersedes all previous ISCN recommendations. There are several new features in ISCN 2013: an update of the microarray nomenclature, many more illustrative examples of uses of nomenclature in all sections some definitions including chromothripsis and duplication a new chapter for nomenclature that can be used for any region-specific assay. The ISCN 2013 is an indispensable reference volume for human cytogeneticists, technicians and students for the interpretation and communication of human cytogenetic nomenclature.

Author : Marcelo L. Larramendy,Sonia Soloneski
Publisher : BoD – Books on Demand
Page : 170 pages
File Size : 47,9 Mb
Release : 2021-07-14
Category : Medical
ISBN : 9781839689413

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Cytogenetics by Marcelo L. Larramendy,Sonia Soloneski Pdf

This edited book, Cytogenetics - Classical and Molecular Strategies for Analysing Heredity Material, presents recent advances in the field of cytogenetics, paying special attention to methodological achievements developed worldwide that have driven the field forward. The contributors clearly discuss several concepts and approaches useful for understanding chromosomal structure and function at its various levels, highlighting chromosomes as visible carriers of heredity material.

Author : Gurdev Khush
Publisher : Elsevier
Page : 316 pages
File Size : 44,6 Mb
Release : 2012-12-02
Category : Medical
ISBN : 9780323156295

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Cytogenetics Of Aneuploids by Gurdev Khush Pdf

Cytogenetics of Aneuploids deals with the cytogenetic aspects of aneuploidy in plants, emphasizing the trisomics, monosomics, and nullisomics and cytogenetics of substitution lines as well as alien additions and substitutions. An account of aneuploidy in animals and man is also given. This volume is organized into 12 chapters and begins with an overview of terminology and chromosomal formulas, along with a brief history of the cytogenetics of aneuploids as a field of enquiry. The next chapters review the entire literature on trisomics, their sources, cytology, transmission rates, genetics, morphology, anatomy, physiology, and biochemistry. The discussion then shifts to monosomics and nullisomics, including their sources and cytology as well as breeding behavior, morphology, and genetic studies. Other uses of monosomics and nullisomics are considered. The following chapters deal with intervarietal substitutions and alien additions and substitutions, emphasizing different methods of producing substitution lines and their utility in genetic analysis and practical plant breeding programs. The book concludes by describing special features of aneuploidy in animals and highlighting specific cases of aneuploidy in the animal kingdom. This book will be of interest to plant breeders and geneticists.

Author : P. K. Gupta
Publisher : Rastogi Publications
Page : 436 pages
File Size : 44,6 Mb
Release : 2007
Category : Cytogenetics
ISBN : 8171337376

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Cytogenetics by P. K. Gupta Pdf