Designer Primer

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PCR Primer Design

Chhandak Basu
PCR Primer Design Book PDF

Author : Chhandak Basu
Publisher : Unknown
Page : 276 pages
File Size : 53,8 Mb
Release : 2021
Category : DNA polymerases
ISBN : 1071617990

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PCR Primer Design by Chhandak Basu Pdf

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.

PCR Primer Design

Anton Yuryev
PCR Primer Design Book PDF

Author : Anton Yuryev
Publisher : Springer Science & Business Media
Page : 870 pages
File Size : 52,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781597455282

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PCR Primer Design by Anton Yuryev Pdf

At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

Microbial Biotechnology- A Laboratory Manual for Bacterial Systems

Surajit Das,Hirak Ranjan Dash
Microbial Biotechnology A Laboratory Manual for Bacterial Systems Book PDF

Author : Surajit Das,Hirak Ranjan Dash
Publisher : Springer
Page : 239 pages
File Size : 48,8 Mb
Release : 2014-11-24
Category : Science
ISBN : 9788132220954

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Microbial Biotechnology- A Laboratory Manual for Bacterial Systems by Surajit Das,Hirak Ranjan Dash Pdf

Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features • Easy to follow by the users as the experiments have been written in simple language and step-wise manner. • Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. • Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. • Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. • Flow-chart of each experiment will quickly guide the users in performing the experiments.

Microbial Biotechnology

Ratul Saikia
Microbial Biotechnology Book PDF

Author : Ratul Saikia
Publisher : New India Publishing
Page : 426 pages
File Size : 53,6 Mb
Release : 2008-01-05
Category : Science
ISBN : 8189422804

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Microbial Biotechnology by Ratul Saikia Pdf

Microbial Biotechnology is wide-ranging, multi-disciplinary activities which include recombinant DNA techniques, cloning and the application of microbes to the production of goods from bread to antibiotics. This book is an attempt to highlight the significant aspects of the vast subject area of microbial biotechnology likes bioinformatics tool for PCR primer designing, fungal biotransformations, bioremediation by microbes, natural products from fungi, microbial diversity etc to provide a complete overview of the subject. It also addresses the role of bacterial plasmid in xenobiotic degradation, antimicrobial resistance in bacteria, ultraviolet-B radiation effect on microbes and human health. The book will be valuable to the researchers, biologist, microbiologist, scientists, post graduate students of microbiology, agriculture, biotechnology and medical science also.

The Engineering Design Primer

K. L. Richards
The Engineering Design Primer Book PDF

Author : K. L. Richards
Publisher : CRC Press
Page : 302 pages
File Size : 46,8 Mb
Release : 2020
Category : Technology & Engineering
ISBN : 0429264917

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The Engineering Design Primer by K. L. Richards Pdf

"Created to support senior-level courses/modules in product design, K. L. Richard's ENGINEERING DESIGN PRIMER reflects the author's deep experience in engineering product management and design. The combination of specific engineering design processes within the boader context of creative, team-based product design, makes this the ideal resource for project-based coursework. Starting with design concepts and tasks, the text then explores materials selection, optimisation, reliability, statistics, testing, and economic factors- all supported with real-life examples. Student readers will gain a practical perspective of the work they'll be doing as their engineering careers begin"--

Autoimmunity

Andras Perl
Autoimmunity Book PDF

Author : Andras Perl
Publisher : Springer Science & Business Media
Page : 432 pages
File Size : 52,8 Mb
Release : 2008-02-01
Category : Medical
ISBN : 9781592598052

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Autoimmunity by Andras Perl Pdf

“Research is to see what everybody else has seen, and to think what nobody else has thought. ” — Albert Szentgyörgyi Autoimmunity: Methods and Protocols is intended to serve as a ready-to-use guide to establish and interrogate human and animal models of autoimmune diseases. The first chapter, “Pathogenesis and Spectrum of Autoimmunity,” discusses major hypotheses driving this most tantalizing area of research since Paul Ehrlich proposed the concept of autoimmunity in 1900. Considering the great diversity and ever-changing spectrum of autoimmunity, it has not been possible to include models and experimental protocols for each known disorder. Rather, several chapters have been devoted to the most prevalent and complex diseases, such as rheumatoid arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, and multiple sclerosis. The chapters are contributed by laboratories actively using the models presented. Each chapter contains an introductory section that discusses the relevance of the model for a particular disease and for autoimmunity in general. Part I contains methods and protocols to assess immunological and biochemical pathways relevant for disease pathogenesis. Chapters in this section focus on methods to identify susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor and signal processing via protein kinases, identification and enumeration of autoantigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Part II contains protocols to establish and assess inflammatory arthritis, systemic lupus erythematosus, myocarditis, thyroiditis, experimental autoimmune encepha- myelitis, insulin-dependent diabetes mellitus, scleroderma, uveitis, and vitiligo.

Essentials of Bioinformatics, Volume I

Noor Ahmad Shaik,Khalid Rehman Hakeem,Babajan Banaganapalli,Ramu Elango
Essentials of Bioinformatics Volume I Book PDF

Author : Noor Ahmad Shaik,Khalid Rehman Hakeem,Babajan Banaganapalli,Ramu Elango
Publisher : Springer
Page : 405 pages
File Size : 43,9 Mb
Release : 2019-03-27
Category : Science
ISBN : 9783030026349

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Essentials of Bioinformatics, Volume I by Noor Ahmad Shaik,Khalid Rehman Hakeem,Babajan Banaganapalli,Ramu Elango Pdf

Bioinformatics is an integrative field of computer science, genetics, genomics, proteomics, and statistics, which has undoubtedly revolutionized the study of biology and medicine in past decades. It mainly assists in modeling, predicting and interpreting large multidimensional biological data by utilizing advanced computational methods. Despite its enormous potential, bioinformatics is not widely integrated into the academic curriculum as most life science students and researchers are still not equipped with the necessary knowledge to take advantage of this powerful tool. Hence, the primary purpose of our book is to supplement this unmet need by providing an easily accessible platform for students and researchers starting their career in life sciences. This book aims to avoid sophisticated computational algorithms and programming. Instead, it mostly focuses on simple DIY analysis and interpretation of biological data with personal computers. Our belief is that once the beginners acquire these basic skillsets, they will be able to handle most of the bioinformatics tools for their research work and to better understand their experimental outcomes. Unlike other bioinformatics books which are mostly theoretical, this book provides practical examples for the readers on state-of-the-art open source tools to solve biological problems. Flow charts of experiments, graphical illustrations, and mock data are included for quick reference. Volume I is therefore an ideal companion for students and early stage professionals wishing to master this blooming field.

Interior Design Materials and Specifications

Lisa Godsey
Interior Design Materials and Specifications Book PDF

Author : Lisa Godsey
Publisher : A&C Black
Page : 433 pages
File Size : 42,6 Mb
Release : 2012-12-14
Category : Design
ISBN : 9781609012298

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Interior Design Materials and Specifications by Lisa Godsey Pdf

Written from the viewpoint of the working designer, this textbook describes each material's characteristics and teaches students how to evaluate, select and specify materials.

The Passivhaus Designer’s Manual

Christina J. Hopfe,Robert S. McLeod
The Passivhaus Designer s Manual Book PDF

Author : Christina J. Hopfe,Robert S. McLeod
Publisher : Routledge
Page : 328 pages
File Size : 42,9 Mb
Release : 2015-10-05
Category : Architecture
ISBN : 9781317536901

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The Passivhaus Designer’s Manual by Christina J. Hopfe,Robert S. McLeod Pdf

Passivhaus is the fastest growing energy performance standard in the world, with almost 50,000 buildings realised to date. Applicable to both domestic and non-domestic building types, the strength of Passivhaus lies in the simplicity of the concept. As European and global energy directives move ever closer towards Zero (fossil) Energy standards, Passivhaus provides a robust ‘fabric first’ approach from which to make the next step. The Passivhaus Designers Manual is the most comprehensive technical guide available to those wishing to design and build Passivhaus and Zero Energy Buildings. As a technical reference for architects, engineers and construction professionals The Passivhaus Designers Manual provides: State of the art guidance for anyone designing or working on a Passivhaus project; In depth information on building services, including high performance ventilation systems and ultra-low energy heating and cooling systems; Holistic design guidance encompassing: daylight design, ecological materials, thermal comfort, indoor air quality and economics; Practical advice on procurement methods, project management and quality assurance; Renewable energy systems suitable for Passivhaus and Zero Energy Buildings; Practical case studies from the UK, USA, and Germany amongst others; Detailed worked examples to show you how it’s done and what to look out for; Expert advice from 20 world renowned Passivhaus designers, architects, building physicists and engineers. Lavishly illustrated with nearly 200 full colour illustrations, and presented by two highly experienced specialists, this is your one-stop shop for comprehensive practical information on Passivhaus and Zero Energy buildings.

Cellular and Biochemical Science

G. Tripathi
Cellular and Biochemical Science Book PDF

Author : G. Tripathi
Publisher : I. K. International Pvt Ltd
Page : 1388 pages
File Size : 41,7 Mb
Release : 2010-03
Category : Medical
ISBN : 9788188237852

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Cellular and Biochemical Science by G. Tripathi Pdf

The fundamental aim underlying Cellular and Biochemical Sciences is to emphasize diversified topics of current interest to postgraduate students pursuing different courses in the area of biological sciences including Zoology, Botany, Biochemistry and Biotechnology. The text is also relevant to the students of Life Sciences, Biosciences, Cell Biology, Bioengineering and Pharmacology. A total of 58 topics have been incorporated in the book and some of the topics are rarely found in other books of Biology. New information has been introduced which updates existing knowledge and enables the book to justify its claim as the most comprehensive text in the sphere of cellular and biochemical sciences at the postgraduate and competitive examination levels. Each and every chapter has been designed in lucid and readable manner. There are references, suggested readings, long questions and objective questions at the end of chapters for revision of topics.

Designer Primer

Tom Porter,Sue Goodman
Designer Primer Book PDF

Author : Tom Porter,Sue Goodman
Publisher : Butterworth-Heinemann
Page : 144 pages
File Size : 52,9 Mb
Release : 1988
Category : Drawing
ISBN : 0408043180

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Designer Primer by Tom Porter,Sue Goodman Pdf

This fully illustrated manual of objective drawing and design drafting skills has been designed specifically for students of architecture and the arts. The novice is introduced to drawing mediums and their rendering potentials, along with a variety of useful methods for achieving drawing accuracy.

Estimating and Costing for Interior Designers

Diana Allison
Estimating and Costing for Interior Designers Book PDF

Author : Diana Allison
Publisher : Bloomsbury Publishing USA
Page : 875 pages
File Size : 53,8 Mb
Release : 2021-02-11
Category : Design
ISBN : 9781501361074

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Estimating and Costing for Interior Designers by Diana Allison Pdf

Math is an essential component of the interior design profession. Estimating and Costing for Interior Designers, Second Edition, teaches readers a logical process for calculating materials and estimating the costs of installed products based on their math calculations. Fully updated and revised, this book utilizes step-by-step examples and worksheets to simplify the math used in the interior design field. Sample problems and exercises take the calculations of quantities needed one step further to actually apply material and labor costs, to discover the installed costs of the specified products. Exercises are provided in introductory, intermediate, and advanced levels for all types of interior designers. Clear sections cover wall and ceiling treatments, window treatments, soft fabrications, upholstery, flooring, and cabinetry and countertops, making this book applicable to both commercial and residential design projects. New to This Edition -Key pedagogical features including: learning objectives, key terms, chapter summaries, professional tips, and glossary. -Student STUDIO materials including: calculation worksheets, schedules/cost worksheets, practice examples, and flashcards. -Robust Instructor Resources including: a revised instructor's guide, test questions, additional practice exercises and answers, PowerPoints lecture slides, and Excel worksheets.

Designer primer

Sue Goodman,Tom Porter
Designer primer Book PDF

Author : Sue Goodman,Tom Porter
Publisher : Macmillan Reference USA
Page : 152 pages
File Size : 50,7 Mb
Release : 1988
Category : Architecture
ISBN : WISC:89099266926

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Designer primer by Sue Goodman,Tom Porter Pdf

A concise, practical, fully illustrated manual of objective drawing and design drafting skills for beginners, by the authors of the successful four-volume Manual of Graphic Techniques.

Protocols in Advanced Genomics and Allied Techniques

Aruna Pal
Protocols in Advanced Genomics and Allied Techniques Book PDF

Author : Aruna Pal
Publisher : Springer Nature
Page : 762 pages
File Size : 42,8 Mb
Release : 2021-11-14
Category : Science
ISBN : 9781071618189

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Protocols in Advanced Genomics and Allied Techniques by Aruna Pal Pdf

This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.

Plant Pathology

Christian Joseph Cumagun
Plant Pathology Book PDF

Author : Christian Joseph Cumagun
Publisher : BoD – Books on Demand
Page : 378 pages
File Size : 41,9 Mb
Release : 2012-04-04
Category : Medical
ISBN : 9789535104896

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Plant Pathology by Christian Joseph Cumagun Pdf

Plant pathology is an applied science that deals with the nature, causes and control of plant diseases in agriculture and forestry. The vital role of plant pathology in attaining food security and food safety for the world cannot be overemphasized.