Dna Arrays

Author : Jang B. Rampal
Publisher : Springer Science & Business Media
Page : 268 pages
File Size : 55,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592340

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DNA Arrays by Jang B. Rampal Pdf

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

Author : Elena V. Grigorenko
Publisher : CRC Press
Page : 183 pages
File Size : 54,8 Mb
Release : 2001-12-21
Category : Science
ISBN : 9781420038859

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DNA Arrays by Elena V. Grigorenko Pdf

A quantum leap in technology took place a few years ago with the introduction of cDNA arrays that have been developed in response to the need for simultaneous analysis of the patterns of expression of thousands of genes. DNA Arrays: Technologies and Experimental Strategies offers a view of different aspects of this rapidly developing technology, in

Author : Pierre Baldi,G. Wesley Hatfield
Publisher : Cambridge University Press
Page : 238 pages
File Size : 42,8 Mb
Release : 2011-04-28
Category : Science
ISBN : 1139437607

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DNA Microarrays and Gene Expression by Pierre Baldi,G. Wesley Hatfield Pdf

Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.

Author : B.R. Jordan
Publisher : Springer Science & Business Media
Page : 139 pages
File Size : 49,9 Mb
Release : 2013-12-01
Category : Medical
ISBN : 9783642565175

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DNA Microarrays: Gene Expression Applications by B.R. Jordan Pdf

This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book.

Author : Richard M. Simon,Edward L. Korn,Lisa M. McShane,Michael D. Radmacher,George W. Wright,Yingdong Zhao
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 49,5 Mb
Release : 2006-05-09
Category : Medical
ISBN : 9780387218663

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Design and Analysis of DNA Microarray Investigations by Richard M. Simon,Edward L. Korn,Lisa M. McShane,Michael D. Radmacher,George W. Wright,Yingdong Zhao Pdf

The analysis of gene expression profile data from DNA micorarray studies are discussed in this book. It provides a review of available methods and presents it in a manner that is intelligible to biologists. It offers an understanding of the design and analysis of experiments utilizing microarrays to benefit scientists. It includes an Appendix tutorial on the use of BRB-ArrayTools and step by step analyses of several major datasets using this software which is available from the National Cancer Institute.

Author : Mark Schena
Publisher : Practical Approach Series
Page : 236 pages
File Size : 55,8 Mb
Release : 1999
Category : Medical
ISBN : 0199637768

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DNA Microarrays by Mark Schena Pdf

DNA Microarrays: A Practical Approach is the first comprehensive overview of an exciting and powerful new technology. DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA, providing a massive parallel platform for data gathering and representing a fundamental technical advance in biomedical research. Such biochips gather data at an unprecedented rate by enabling the use of advanced fabrication, detection, and data mining technologies. Written and edited by experts in the field, this book provides fascinating insight into this remarkable advancement. It opens with an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips, and continues with descriptions of the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. The chapters address many topics, among them assay optimization, antisense scanning arrays, the manufacture of molecular arrays, and gene expression analysis. Also addressed are the uses of expression data in bioinformatics, of active microelectronic arrays for DNA hybridization analysis, and of microarray technology in pharmacogenomics. DNA Microarrays: A Practical Approach is ideal for researchers investigating patterns of gene expression (and the relationship with disease), and it is essential for any researcher interested in the use of biochips.

Author : Anonim
Publisher : Elsevier
Page : 218 pages
File Size : 47,9 Mb
Release : 2004-12-10
Category : Medical
ISBN : 9780080495514

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DNA Arrays in Neurobiology by Anonim Pdf

DNA array technology is a technique for studying gene expression by comparing samples of different genes. The result is an enormous amount of data that must be carefully analyzed in order for it to be useful and meaningful. This book examines both data analysis and techniques for ensuring optimal experimental conditions. The array approach has applications in a number of model systems, including development, learning and drug abuse. In addition, the technique has applications in a number of neurological disorders such as Alzheimer's disease, schizophrenia, multiple sclerosis, and neurological cancers.

Author : Mark Schena
Publisher : Scion Publishing Ltd
Page : 289 pages
File Size : 48,9 Mb
Release : 2007-10-01
Category : Science
ISBN : 9781907904394

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DNA Microarrays by Mark Schena Pdf

DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. This book is a 'must have' manual for researchers in all fields of biology, medicine and agriculture.

Author : Steen Knudsen
Publisher : John Wiley & Sons
Page : 194 pages
File Size : 40,5 Mb
Release : 2005-03-04
Category : Science
ISBN : 9780471670261

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Guide to Analysis of DNA Microarray Data by Steen Knudsen Pdf

Written for biologists and medical researchers who don't have any special training in data analysis and statistics, Guide to Analysis of DNA Microarray Data, Second Edition begins where DNA array equipment leaves off: the image produced by the microarray. The text deals with the questions that arise starting at this point, providing an introduction to microarray technology, then moving on to image analysis, data analysis, cluster analysis, and beyond. With all chapters rewritten, updated, and expanded to include the latest generation of technology and methods, Guide to Analysis of DNA Microarray Data, Second Edition offers practitioners reliable information using concrete examples and a clear, comprehensible style. This Second Edition features entirely new chapters on: * Image analysis * Experiment design * Automated analysis, integrated analysis, and systems biology * Interpretation of results Intended for readers seeking practical applications, this text covers a broad spectrum of proven approaches in this rapidly growing technology. Additional features include further reading suggestions for each chapter, as well as a thorough review of available analysis software.

Author : Ulrike Nuber
Publisher : Taylor & Francis
Page : 320 pages
File Size : 41,8 Mb
Release : 2007-01-24
Category : Science
ISBN : 9781134243990

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DNA Microarrays by Ulrike Nuber Pdf

"DNA Microarrays" introduces the reader to the different types of DNA microarrays and their various applications. Furthermore, several data analysis methods are described. It is written for scientists who are entering the field of DNA microarrays, as well as for those already familiar with the technology, but interested in new applications and methods.

Author : Anonim
Publisher : Elsevier
Page : 512 pages
File Size : 43,5 Mb
Release : 2006-08-28
Category : Science
ISBN : 9780080464664

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DNA Microarrays, Part B: Databases and Statistics by Anonim Pdf

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis

Author : David B. Allison,Grier P. Page,T. Mark Beasley,Jode W. Edwards
Publisher : CRC Press
Page : 391 pages
File Size : 54,8 Mb
Release : 2005-11-14
Category : Mathematics
ISBN : 9781420028799

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DNA Microarrays and Related Genomics Techniques by David B. Allison,Grier P. Page,T. Mark Beasley,Jode W. Edwards Pdf

Considered highly exotic tools as recently as the late 1990s, microarrays are now ubiquitous in biological research. Traditional statistical approaches to design and analysis were not developed to handle the high-dimensional, small sample problems posed by microarrays. In just a few short years the number of statistical papers providing approaches

Author : Steen Knudsen
Publisher : John Wiley & Sons
Page : 148 pages
File Size : 50,8 Mb
Release : 2011-09-23
Category : Science
ISBN : 9780471461180

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A Biologist's Guide to Analysis of DNA Microarray Data by Steen Knudsen Pdf

A great introductory book that details reliable approaches to problems met in standard microarray data analyses. It provides examples of established approaches such as cluster analysis, function prediction, and principle component analysis. Discover real examples to illustrate the key concepts of data analysis. Written for those without any advanced background in math, statistics, or computer sciences, this book is essential for anyone interested in harnessing the immense potential of microarrays in biology and medicine.

Author : Anonim
Publisher : Elsevier
Page : 512 pages
File Size : 41,7 Mb
Release : 2011-08-19
Category : Science
ISBN : 0080464653

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DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols by Anonim Pdf

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta-analysis

Author : Anonim
Publisher : Taylor & Francis
Page : 321 pages
File Size : 53,7 Mb
Release : 2024-06-30
Category : Electronic
ISBN : 9781134182749

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Microarray Technology Through Applications by Anonim Pdf