Microarray Technology Through Applications

Author : Francesco Falciani
Publisher : Garland Science
Page : 276 pages
File Size : 41,8 Mb
Release : 2007-06-30
Category : Science
ISBN : 9780203967270

Get Book

Microarray Technology Through Applications by Francesco Falciani Pdf

Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536

Author : Anonim
Publisher : Taylor & Francis
Page : 321 pages
File Size : 47,7 Mb
Release : 2024-07-01
Category : Electronic
ISBN : 9781134182749

Get Book

Microarray Technology Through Applications by Anonim Pdf

Author : Uwe R. Müller,Dan V. Nicolau
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 52,5 Mb
Release : 2006-03-30
Category : Medical
ISBN : 9783540265788

Get Book

Microarray Technology and Its Applications by Uwe R. Müller,Dan V. Nicolau Pdf

Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications that are likely to have a dominating in?uence on the development of our society during this century. Given the process by which science builds on prior knowledge, it is perhaps unfair to single out a few inventions and credit them with having contributed most to thisavalancheofknowledge. Yet,therearesurelysomethatwillberecognized as having had a more profound impact than others, not just in the furthering of our scienti?c knowledge, but by leveraging commercial applications that provide a tangible return to our society. The now famous Polymerase Chain Reaction, or PCR, is surely one of those, as it has uniquely catalyzed molecular biology during the past 20 years, and continues to have a signi?cant impact on all areas that involve nucleic acids, ranging from molecular pathology to forensics. Ten years ago micro- ray technology emerged as a new and powerful tool to study nucleic acid - quences in a highly multiplexed manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues.

Author : Paul C.H. Li,Abootaleb Sedighi,Lin Wang
Publisher : Humana
Page : 0 pages
File Size : 48,7 Mb
Release : 2015-11-28
Category : Medical
ISBN : 1493931350

Get Book

Microarray Technology by Paul C.H. Li,Abootaleb Sedighi,Lin Wang Pdf

This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.

Author : James V. Rogers
Publisher : Nova Biomedical Books
Page : 0 pages
File Size : 42,5 Mb
Release : 2014
Category : Analytical biochemistry
ISBN : 1629486698

Get Book

Microarrays by James V. Rogers Pdf

The sequencing of the human genome was a pivotal event in science that opened the door to exploring the structure and function of genes and their regulation. The activation or suppression of transcribed genes is critical to the orchestration of everyday biological processes at the cellular, tissue, and physiological levels. The rapid advance of science and technology has yielded the development of the microarray technique, which has propelled a much deeper understanding of the genome. By combining these technological advances in microarrays with statistical and bioinformatics software, investigators are now able to perform scientific investigations geared towards answering unique biological problems that encompass many fields of research from genetics and evolution to molecular medicine, health, and disease. This book provides an overview of the use and application of microarrays throughout the life sciences to address diverse complex biological questions. In this collection, authors present information on using microarrays to unlock molecular mechanisms and gene expression patterns associated with research areas ranging from biodefense, pathological changes, and monitoring antimicrobial resistance genes to diagnostics, marine biodiversity, and dermal toxicology.

Author : Jang B. Rampal
Publisher : Springer Science & Business Media
Page : 268 pages
File Size : 53,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592340

Get Book

DNA Arrays by Jang B. Rampal Pdf

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

Author : Robert S. Matson
Publisher : CRC Press
Page : 325 pages
File Size : 51,5 Mb
Release : 2013-03-13
Category : Medical
ISBN : 9781439855638

Get Book

Applying Genomic and Proteomic Microarray Technology in Drug Discovery, Second Edition by Robert S. Matson Pdf

Microarrays play an increasingly significant role in drug discovery. The commercial landscape has changed dramatically over the past few years and researchers have made great advancements with regard to construction and use. Now in its second edition, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. Updated and revised to reflect recent progress in the field, the second edition discusses: Expanded omics-driven applications, including the areas of metabolomics and chemical biology The commercialization of the microarray platform, with a historical perspective aimed at recognizing key technological developments Solid-supports (substrates) and surface chemistries currently used in the creation of nucleic acid and protein microarrays Different approaches to producing microarrays that achieve spot equality with the same number of molecules properly oriented The development of the gene expression microarray and representative applications The development of protein microarray technology, including its history and key applications Unique to this edition is a new chapter on multiplex assays that examines the development and applications of arrays across diverse platforms. It discusses applications for qPCR, multiplex lateral flow, and multiplex bead assays. It also presents platform-to-platform comparisons. Microarrays remain an invaluable tool for omics-based research not only in drug discovery, but in the life sciences, in clinical research, and for diagnostic applications worldwide. This volume presents the current state of the art on the utility of this technology to solve a host of important biological problems.

Author : Robert S. Matson
Publisher : CRC Press
Page : 260 pages
File Size : 42,8 Mb
Release : 2004-12-28
Category : Medical
ISBN : 9780203491133

Get Book

Applying Genomic and Proteomic Microarray Technology in Drug Discovery by Robert S. Matson Pdf

Microarrays play an increasingly significant role in drug discovery. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. The author addresses the drawbacks, helping

Author : Jang B. Rampal
Publisher : Springer Science & Business Media
Page : 927 pages
File Size : 43,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781597453042

Get Book

Microarrays by Jang B. Rampal Pdf

This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.

Author : Richard M. Simon,Edward L. Korn,Lisa M. McShane,Michael D. Radmacher,George W. Wright,Yingdong Zhao
Publisher : Springer Science & Business Media
Page : 205 pages
File Size : 42,9 Mb
Release : 2006-05-09
Category : Medical
ISBN : 9780387218663

Get Book

Design and Analysis of DNA Microarray Investigations by Richard M. Simon,Edward L. Korn,Lisa M. McShane,Michael D. Radmacher,George W. Wright,Yingdong Zhao Pdf

The analysis of gene expression profile data from DNA micorarray studies are discussed in this book. It provides a review of available methods and presents it in a manner that is intelligible to biologists. It offers an understanding of the design and analysis of experiments utilizing microarrays to benefit scientists. It includes an Appendix tutorial on the use of BRB-ArrayTools and step by step analyses of several major datasets using this software which is available from the National Cancer Institute.

Author : Daniel P. Berrar,Werner Dubitzky,Martin Granzow
Publisher : Springer Science & Business Media
Page : 382 pages
File Size : 51,9 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306478154

Get Book

A Practical Approach to Microarray Data Analysis by Daniel P. Berrar,Werner Dubitzky,Martin Granzow Pdf

In the past several years, DNA microarray technology has attracted tremendous interest in both the scientific community and in industry. With its ability to simultaneously measure the activity and interactions of thousands of genes, this modern technology promises unprecedented new insights into mechanisms of living systems. Currently, the primary applications of microarrays include gene discovery, disease diagnosis and prognosis, drug discovery (pharmacogenomics), and toxicological research (toxicogenomics). Typical scientific tasks addressed by microarray experiments include the identification of coexpressed genes, discovery of sample or gene groups with similar expression patterns, identification of genes whose expression patterns are highly differentiating with respect to a set of discerned biological entities (e.g., tumor types), and the study of gene activity patterns under various stress conditions (e.g., chemical treatment). More recently, the discovery, modeling, and simulation of regulatory gene networks, and the mapping of expression data to metabolic pathways and chromosome locations have been added to the list of scientific tasks that are being tackled by microarray technology. Each scientific task corresponds to one or more so-called data analysis tasks. Different types of scientific questions require different sets of data analytical techniques. Broadly speaking, there are two classes of elementary data analysis tasks, predictive modeling and pattern-detection. Predictive modeling tasks are concerned with learning a classification or estimation function, whereas pattern-detection methods screen the available data for interesting, previously unknown regularities or relationships.

Author : Martin Dufva
Publisher : Humana Press
Page : 0 pages
File Size : 51,5 Mb
Release : 2011-01-26
Category : Science
ISBN : 1617379417

Get Book

DNA Microarrays for Biomedical Research by Martin Dufva Pdf

DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology.

Author : Dov Stekel
Publisher : Cambridge University Press
Page : 296 pages
File Size : 40,7 Mb
Release : 2003-09-08
Category : Medical
ISBN : 052152587X

Get Book

Microarray Bioinformatics by Dov Stekel Pdf

This book is a comprehensive guide to all of the mathematics, statistics and computing you will need to successfully operate DNA microarray experiments. It is written for researchers, clinicians, laboratory heads and managers, from both biology and bioinformatics backgrounds, who work with, or who intend to work with microarrays. The book covers all aspects of microarray bioinformatics, giving you the tools to design arrays and experiments, to analyze your data, and to share your results with your organisation or with the international community. There are chapters covering sequence databases, oligonucleotide design, experimental design, image processing, normalisation, identifying differentially expressed genes, clustering, classification and data standards. The book is based on the highly successful Microarray Bioinformatics course at Oxford University, and therefore is ideally suited for teaching the subject at postgraduate or professional level.

Author : Gary Hardiman
Publisher : CRC Press
Page : 362 pages
File Size : 55,5 Mb
Release : 2009-04-02
Category : Medical
ISBN : 9781420094510

Get Book

Microarray Innovations by Gary Hardiman Pdf

In recent years, high-density DNA microarrays have revolutionized biomedical research and drug discovery efforts by the pharmaceutical industry. Their efficacy in identifying and prioritizing drug targets based on their ability to confirm a large number of gene expression measurements in parallel has become a key element in drug discovery. Microarr

Author : Steve Russell,Lisa A. Meadows,Roslin R. Russell
Publisher : Academic Press
Page : 460 pages
File Size : 40,8 Mb
Release : 2008-11-21
Category : Science
ISBN : 9780080919768

Get Book

Microarray Technology in Practice by Steve Russell,Lisa A. Meadows,Roslin R. Russell Pdf

Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays