Dna Cloning

Author : Seok-Yong Choi,Hyunju Ro,Hankuil Yi
Publisher : Springer
Page : 131 pages
File Size : 50,8 Mb
Release : 2019-04-17
Category : Medical
ISBN : 9789402416626

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DNA Cloning: A Hands-on Approach by Seok-Yong Choi,Hyunju Ro,Hankuil Yi Pdf

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Author : National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Engineering, and Public Policy
Publisher : National Academies Press
Page : 295 pages
File Size : 44,5 Mb
Release : 2002-06-17
Category : Science
ISBN : 9780309076371

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Scientific and Medical Aspects of Human Reproductive Cloning by National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Engineering, and Public Policy Pdf

Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.

Author : T. A. Brown
Publisher : John Wiley & Sons
Page : 337 pages
File Size : 54,7 Mb
Release : 2013-04-25
Category : Science
ISBN : 9781118685280

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Gene Cloning and DNA Analysis by T. A. Brown Pdf

Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)

Author : David M. Glover
Publisher : Springer Science & Business Media
Page : 79 pages
File Size : 55,6 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401576468

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Genetic Engineering Cloning DNA by David M. Glover Pdf

Author : Sunil Chandran,Kevin W. George
Publisher : Humana
Page : 334 pages
File Size : 41,6 Mb
Release : 2021-09-02
Category : Medical
ISBN : 1071609106

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DNA Cloning and Assembly by Sunil Chandran,Kevin W. George Pdf

This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : Dominic Wong
Publisher : Springer Science & Business Media
Page : 227 pages
File Size : 41,9 Mb
Release : 2007-12-31
Category : Science
ISBN : 9780387286792

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The ABCs of Gene Cloning by Dominic Wong Pdf

Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.

Author : Svein Valla,Rahmi Lale
Publisher : Humana Press
Page : 0 pages
File Size : 43,9 Mb
Release : 2014-01-07
Category : Medical
ISBN : 1627037632

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DNA Cloning and Assembly Methods by Svein Valla,Rahmi Lale Pdf

In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.

Author : Karl Drlica
Publisher : John Wiley & Sons
Page : 388 pages
File Size : 47,8 Mb
Release : 2004
Category : Science
ISBN : UOM:39015056212825

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Understanding DNA and Gene Cloning by Karl Drlica Pdf

Publisher's description: With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies.

Author : David M. Glover
Publisher : Springer
Page : 231 pages
File Size : 51,8 Mb
Release : 2013-12-19
Category : Medical
ISBN : 9781489932464

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Gene Cloning by David M. Glover Pdf

This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.

Author : Julia Lodge,Peter Lund,Steve Minchin
Publisher : Garland Science
Page : 612 pages
File Size : 51,6 Mb
Release : 2007-01-24
Category : Science
ISBN : 9781135728922

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Gene Cloning by Julia Lodge,Peter Lund,Steve Minchin Pdf

The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.

Author : Christopher Howe
Publisher : Cambridge University Press
Page : 250 pages
File Size : 52,6 Mb
Release : 2007-07-12
Category : Medical
ISBN : 9781139463584

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Gene Cloning and Manipulation by Christopher Howe Pdf

Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Author : Terence A. Brown
Publisher : Nelson Thornes
Page : 0 pages
File Size : 42,5 Mb
Release : 1998
Category : Cloning
ISBN : 0748740708

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Gene Cloning by Terence A. Brown Pdf

Gene Cloning provides a basic introduction for students and researchers who have no previous experience of experiments with DNA, and assumes very little prior knowledge on the part of the reader. A three part structure addresses the basic principles of gene cloning, the application of cloning in gene analysis, and the role of gene cloning in research and biotechnology. The book is written in clear, jargon-free language, and is extensively illustrated with two-color line drawings.

Author : T. A. Brown
Publisher : John Wiley & Sons
Page : 374 pages
File Size : 41,7 Mb
Release : 2016-01-19
Category : Science
ISBN : 9781119072560

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Gene Cloning and DNA Analysis by T. A. Brown Pdf

Known world-wide as the standard introductory text to this important and exciting area, the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the chapters on DNA sequencing and genome studies have been rewritten to reflect the continuing rapid developments in this area of DNA analysis: In depth description of the next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes New material on the use of ChiP-seq to locate protein-binding sites Extended coverage of the strategies used to assemble genome sequences Description of how the Neanderthal genome has been sequenced and what that sequence tells us about interbreeding between Neanderthals and Homo sapiens Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.

Author : David M. Glover
Publisher : Oxford University Press, USA
Page : 282 pages
File Size : 43,5 Mb
Release : 1985
Category : Language Arts & Disciplines
ISBN : UOM:39015020767839

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DNA Cloning by David M. Glover Pdf

Spplication of plasmids containing promoters specific for phage-encoded RNA polymerases; Chice and use of cosmid vectors; Genetic approaches to the cloning, modification and characterization of cosmid clones and clone libraries; The purification on eucaryotic polypeptides expressed in Escherichia coli; Production and prufication of polyclonal antibodies to the foreign segment of B-galactosidase fusion proteins; Production of monoclonal antibodies against fusion proteins produced in Escherichia coli; Expression and secretion of foreign genes in yeast; The use of vectors based of gene amplification for the expression of cloned genes in mammalian cells; Retroviral vectors; The production of transgenic mice by the microinjection of cloned DNA into fertilized one-cell eggs.

Author : Bing-Yuan Chen,Harry W. Janes
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 51,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591770

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PCR Cloning Protocols by Bing-Yuan Chen,Harry W. Janes Pdf

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.