Dna Cloning A Hands On Approach

Author : Seok-Yong Choi,Hyunju Ro,Hankuil Yi
Publisher : Springer
Page : 131 pages
File Size : 45,5 Mb
Release : 2019-04-17
Category : Medical
ISBN : 9789402416626

Get Book

DNA Cloning: A Hands-on Approach by Seok-Yong Choi,Hyunju Ro,Hankuil Yi Pdf

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Author : Seok-Yong Choi,Hyunju Ro,Hankuil Yi
Publisher : Unknown
Page : 139 pages
File Size : 46,6 Mb
Release : 2019
Category : Clone cells
ISBN : 9402416617

Get Book

DNA Cloning by Seok-Yong Choi,Hyunju Ro,Hankuil Yi Pdf

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Author : David M. Glover,B. D. Hames
Publisher : Unknown
Page : 255 pages
File Size : 53,8 Mb
Release : 1996
Category : Molecular cloning
ISBN : OCLC:37819776

Get Book

DNA Cloning by David M. Glover,B. D. Hames Pdf

Author : Ralph Rapley,John M. Walker
Publisher : Springer Science & Business Media
Page : 708 pages
File Size : 53,6 Mb
Release : 1998-07-13
Category : Science
ISBN : 9780896035010

Get Book

Molecular Biomethods Hand Book by Ralph Rapley,John M. Walker Pdf

The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.

Author : National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on Mapping and Sequencing the Human Genome
Publisher : National Academies Press
Page : 128 pages
File Size : 51,5 Mb
Release : 1988-01-01
Category : Science
ISBN : 9780309038409

Get Book

Mapping and Sequencing the Human Genome by National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on Mapping and Sequencing the Human Genome Pdf

There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.

Author : Joseph Sambrook
Publisher : Unknown
Page : 0 pages
File Size : 44,6 Mb
Release : 2003
Category : Eukaryotic cells
ISBN : 0879695773

Get Book

Molecular Cloning by Joseph Sambrook Pdf

Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 40,9 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459

Get Book

Molecular Biology Techniques by Heather Miller,D. Scott Witherow,Sue Carson Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Anil Kumar Anal
Publisher : CRC Press
Page : 144 pages
File Size : 49,6 Mb
Release : 2018-02-02
Category : Medical
ISBN : 9781351643634

Get Book

Bionanotechnology by Anil Kumar Anal Pdf

This book deals with a subject of high interest and importance in all sectors, including biomedical, food, agriculture, energy, and environment. Biological systems are essential in nanotechnology, and many new applications are being developed by mimicking the natural systems. Approaching these topics from an engineering perspective, the book offers insight on the details of nanoscale fabrication processes as well as cell biology. The basics of biology and chemistry, with a focus on how to engineer the behavior of molecules at the nanoscale, are also explored and analyzed. The aim of the text is to provide the reader with broader knowledge of biological methods for signal transduction and molecular recognitions systems and how they can be replicated in bio-sensing applications. The reader will learn the basic structures and interactions of biomacromolecules for developing biocompatible and eco-friendly devices.

Author : Dr. B. Usharani
Publisher : Notion Press
Page : 164 pages
File Size : 40,9 Mb
Release : 2019-04-05
Category : Science
ISBN : 9781645465119

Get Book

A Practical Hand Book of Genes to Clones -Manual by Dr. B. Usharani Pdf

To the student: There are a number of features to help you learn as you read. Each section is summarized with a bulleted list of key concepts. Key terms are highlighted in boldface in the text and defined in the margin for easy reference. Each chapter focuses on historical perspectives, methods, techniques and medical applications. Finally, each chapter concludes with suggested further reading, a brief list of current reviews and pivotal papers to supplement and reinforce the chapter content.

Author : B.C. Schaefer
Publisher : Taylor & Francis
Page : 181 pages
File Size : 52,9 Mb
Release : 2023-04-28
Category : Science
ISBN : 9781000950083

Get Book

Gene Cloning and Analysis by B.C. Schaefer Pdf

This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic

Author : Chittaranjan Kole
Publisher : Springer Nature
Page : 340 pages
File Size : 46,7 Mb
Release : 2021-08-31
Category : Science
ISBN : 9783030758790

Get Book

Genomic Designing for Biotic Stress Resistant Cereal Crops by Chittaranjan Kole Pdf

This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the biotic stresses caused by different diseases and pests that are important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in cereal crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to biotic stresses. The eight chapters each dedicated to a cereal crop in this volume elucidate on different types of biotic stresses and their effects on and interaction with the crop; enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing biotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate on different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genes for generating biotic stress-resistant crops.

Author : William S. M. Wold,Ann E. Tollefson
Publisher : Springer Science & Business Media
Page : 246 pages
File Size : 50,5 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781597451666

Get Book

Adenovirus Methods and Protocols by William S. M. Wold,Ann E. Tollefson Pdf

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.

Author : Christopher Howe
Publisher : Cambridge University Press
Page : 250 pages
File Size : 47,8 Mb
Release : 2007-07-12
Category : Medical
ISBN : 9781139463584

Get Book

Gene Cloning and Manipulation by Christopher Howe Pdf

Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Author : MCPHERSON.,S. G. Møller
Publisher : Garland Science
Page : 304 pages
File Size : 47,9 Mb
Release : 2000
Category : Juvenile Nonfiction
ISBN : 9781859960172

Get Book

PCR by MCPHERSON.,S. G. Møller Pdf

A thoroughly updated version of the successful first edition, with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections.

Author : Dorothee Langel
Publisher : Cuvillier Verlag
Page : 227 pages
File Size : 52,8 Mb
Release : 2008
Category : Electronic
ISBN : 9783867276467

Get Book

Biosynthesis of the Unique Necic Acid Moiety in Lycopsamine Type Pyrrolizidine Alkaloids by Dorothee Langel Pdf