Early Detection Of Cyanobacterial Toxins Using Genetic Methods

Author : J. Paul Rasmussen,Paul T. Monis,Christopher P. Saint
Publisher : American Water Works Association
Page : 160 pages
File Size : 49,7 Mb
Release : 2008
Category : Bacterial pollution of water
ISBN : UCLA:L0101089936

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Early Detection of Cyanobacterial Toxins Using Genetic Methods by J. Paul Rasmussen,Paul T. Monis,Christopher P. Saint Pdf

This research sought to develop and test rapid genetic methods to identify toxic cyanobacteria. The key objectives were to (1) conduct a literature review and industry questionnaire examining options for rapid genetic tests; (2) characterize and understand the genes involved in cylindrospermopsin and anatoxin production; (3) adapt conventional PCR assays to real-time PCR; (4) develop rapid field methods for DNA preparation; and (5) develop probes for toxin genes and testing in the field.

Author : N. Thajuddin,A. Sankara narayanan,D. Dhanasekaran
Publisher : Springer Nature
Page : 504 pages
File Size : 43,9 Mb
Release : 2024-01-19
Category : Science
ISBN : 9789819945146

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Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin by N. Thajuddin,A. Sankara narayanan,D. Dhanasekaran Pdf

This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book. In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin. Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research. ​

Author : G A Codd,T M Jefferies,C W Keevil,E Potter
Publisher : Elsevier
Page : 202 pages
File Size : 43,9 Mb
Release : 1994-01-01
Category : Technology & Engineering
ISBN : 9781845698164

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Detection Methods for Cynobacterial toxins by G A Codd,T M Jefferies,C W Keevil,E Potter Pdf

Cyanobacteria, or blue-green algae, commonly occur in a variety of water types throughout the world. A variable, but, high proportion of the cyanobacterial blooms and scums, which can develop annually in lakes, reservoirs, canals and slow-flowing rivers, contain potent toxins. Although animal poisonings and human health problems associated with the ingestion of, or contact with, cyanobacterial scums have long been recognized, a developing understanding of the health hazards posed by the toxins requires that reliable, sensitive, specific and convenient methods are available for their detection and quantification. Detection methods for cynobacterial toxins looks at the application of biological, toxicological, biochemical and physicochemical techniques in studies of cyanobacterial toxins and at experimental methods that have identified at least 50 cyanobacterial compounds toxic to vertebrates. Academics, public health bodies, environmental protection agencies and water companies are all involved in monitoring cyanobacterial levels and effects, and this book will help to ensure that their research is not duplicated, is standardized and comparable, and that optimal progress can be made in an important area that is still in its developing stages.

Author : Rainer Kurmayer,Kaarina Sivonen,Annick Wilmotte,Nico Salmaso
Publisher : John Wiley & Sons
Page : 446 pages
File Size : 44,8 Mb
Release : 2017-09-05
Category : Science
ISBN : 9781119332107

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Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria by Rainer Kurmayer,Kaarina Sivonen,Annick Wilmotte,Nico Salmaso Pdf

A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

Author : Jussi Meriluoto,Lisa Spoof,Geoffrey A. Codd
Publisher : John Wiley & Sons
Page : 576 pages
File Size : 53,5 Mb
Release : 2017-01-30
Category : Science
ISBN : 9781119068686

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Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis by Jussi Meriluoto,Lisa Spoof,Geoffrey A. Codd Pdf

A valuable handbook containing reviews, practical methods and standard operating procedures. A valuable and practical working handbook containing introductory and specialist content that tackles a major and growing field of environmental, microbiological and ecotoxicological monitoring and analysis Includes introductory reviews, practical analytical chapters and a comprehensive listing of almost thirty Standard Operating Procedures (SOPs) For use in the laboratory, in academic and government institutions and industrial settings Those readers will appreciate the research that validates and updates cyanotoxin monitoring and analysis plus adding to approaches for setting standard methods that can be applied worldwide. Wayne Carmichael, Analytical and Bioanalytical Chemistry (2018)

Author : Rainer Kurmayer,Kaarina Sivonen,Annick Wilmotte,Nico Salmaso
Publisher : John Wiley & Sons
Page : 440 pages
File Size : 46,9 Mb
Release : 2017-06-20
Category : Science
ISBN : 9781119332121

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Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria by Rainer Kurmayer,Kaarina Sivonen,Annick Wilmotte,Nico Salmaso Pdf

A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

Author : B. Nicholson
Publisher : Unknown
Page : 172 pages
File Size : 45,5 Mb
Release : 2007-01-01
Category : Science
ISBN : 1843398265

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Determination and Significance of Emerging Algal Toxins (cyanotoxins) by B. Nicholson Pdf

Author : D. P. Singh,Gurpreet Kaur
Publisher : I. K. International Pvt Ltd
Page : 267 pages
File Size : 47,7 Mb
Release : 2009
Category : Algae
ISBN : 9789380026190

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Algal Biology and Biotechnology by D. P. Singh,Gurpreet Kaur Pdf

In the recent past, many advances have been made in the field of biology and biotechnology of algae, especially microalgae. This book includes chapters on taxonomy, diversity and physiology of blue-green algae as these organisms are most important from biotechnological point of view. Use of algae as biofertilizer, source of natural colours, bioactive compounds, phytochemicals with pharmaceutical and biotechnological applications, food and feed has been discussed. Environmental pollution is the major problem all over the world. The potential of algae in combating water pollution is also highlighted. Depleting fossil fuel is another concern and it is felt that there is a need for alternative renewable resources. Algae as a potential source of biofuel are also discussed in this book

Author : H. Kenneth Hudnell
Publisher : Springer Science & Business Media
Page : 950 pages
File Size : 49,9 Mb
Release : 2008-03-13
Category : Medical
ISBN : 9780387758657

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Cyanobacterial Harmful Algal Blooms: State of the Science and Research Needs by H. Kenneth Hudnell Pdf

With the ever-increasing incidence of harmful cyanobacterial algal blooms, this monograph has added urgency and will be essential reading for all sorts of researchers, from neuroscientists to cancer research specialists. The volume contains the proceedings of the 2005 International Symposium on Cyanobacterial Harmful Algal Blooms, and has been edited by H. Kenneth Hudnell, of the US Environmental Protection Agency. It contains much of the most recent research into the subject.

Author : Ingrid Chorus,Martin Welker
Publisher : CRC Press
Page : 701 pages
File Size : 51,9 Mb
Release : 2021-03-08
Category : Technology & Engineering
ISBN : 9781000262049

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Toxic Cyanobacteria in Water by Ingrid Chorus,Martin Welker Pdf

Cyanobacterial toxins are among the hazardous substances most widely found in water. They occur naturally, but concentrations hazardous to human health are usually due to human activity. Therefore, to protect human health, managing lakes, reservoirs and rivers to prevent cyanobacterial blooms is critical. This second edition of Toxic Cyanobacteria in Water presents the current state of knowledge on the occurrence of cyanobacteria and cyanotoxins as well as their impacts on health through water-related exposure pathways, chiefly drinking-water and recreational activity. It provides scientific and technical background information to support hazard identification, assessment and prioritisation of the risks posed by cyanotoxins, and it outlines approaches for their management at each step of the water-use system. It sets out key practical considerations for developing management strategies, implementing efficient measures and designing monitoring programmes. This enables stakeholders to evaluate whether there is a health risk from toxic cyanobacteria and to mitigate it with appropriate measures. This book is intended for those working on toxic cyanobacteria with a specific focus on public health protection. It intends to empower professionals from different disciplines to communicate and cooperate for sustainable management of toxic cyanobacteria, including public health workers, ecologists, academics, and catchment and waterbody managers. Ingrid Chorus headed the department for Drinking-Water and Swimming-Pool Hygiene at the German Environment Agency. Martin Welker is a limnologist and microbiologist, currently with bioMérieux in Lyon, France.

Author : Ingrid Chorus,Martin Welker
Publisher : CRC Press
Page : 840 pages
File Size : 52,6 Mb
Release : 2021-03-08
Category : Technology & Engineering
ISBN : 9781000262025

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Toxic Cyanobacteria in Water by Ingrid Chorus,Martin Welker Pdf

Cyanobacterial toxins are among the hazardous substances most widely found in water. They occur naturally, but concentrations hazardous to human health are usually due to human activity. Therefore, to protect human health, managing lakes, reservoirs and rivers to prevent cyanobacterial blooms is critical. This second edition of Toxic Cyanobacteria in Water presents the current state of knowledge on the occurrence of cyanobacteria and cyanotoxins as well as their impacts on health through water-related exposure pathways, chiefly drinking-water and recreational activity. It provides scientific and technical background information to support hazard identification, assessment and prioritisation of the risks posed by cyanotoxins, and it outlines approaches for their management at each step of the water-use system. It sets out key practical considerations for developing management strategies, implementing efficient measures and designing monitoring programmes. This enables stakeholders to evaluate whether there is a health risk from toxic cyanobacteria and to mitigate it with appropriate measures. This book is intended for those working on toxic cyanobacteria with a specific focus on public health protection. It intends to empower professionals from different disciplines to communicate and cooperate for sustainable management of toxic cyanobacteria, including public health workers, ecologists, academics, and catchment and waterbody managers. Ingrid Chorus headed the department for Drinking-Water and Swimming-Pool Hygiene at the German Environment Agency. Martin Welker is a limnologist and microbiologist, currently with bioMérieux in Lyon, France.

Author : G. A. Codd,T. M. Jefferies,C. W. Keevil
Publisher : Woodhead Pub Limited
Page : 202 pages
File Size : 44,6 Mb
Release : 1994-01-01
Category : Medical
ISBN : 1855738023

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Detection Methods for Cynobacterial Toxins by G. A. Codd,T. M. Jefferies,C. W. Keevil Pdf

Cyanobacteria, or blue-green algae, commonly occur in a variety of water types throughout the world. A high proportion of the cyanobacterial blooms and scums contain potent toxins. This volume looks at the application of biological, toxicological, biochemical and physicochemical techniques in studies of cyanobacterial toxins and at experimental methods that have identified at least 50 cyanobacterial compounds toxic to vertebrates.

Author : Anonim
Publisher : Newnes
Page : 5036 pages
File Size : 42,7 Mb
Release : 2008-09-01
Category : Science
ISBN : 9780444522726

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Encyclopedia of Environmental Health by Anonim Pdf

Environmental health has evolved over time into a complex, multidisciplinary field. Many of the key determinants and solutions to environmental health problems lie outside the direct realm of health and are strongly dependent on environmental changes, water and sanitation, industrial development, education, employment, trade, tourism, agriculture, urbanization, energy, housing and national security. Environmental risks, vulnerability and variability manifest themselves in different ways and at different time scales. While there are shared global and transnational problems, each community, country or region faces its own unique environmental health problems, the solution of which depends on circumstances surrounding the resources, customs, institutions, values and environmental vulnerability. This work contains critical reviews and assessments of environmental health practices and research that have worked in places and thus can guide programs and economic development in other countries or regions. The Encyclopedia of Environmental Health, Five Volume Set seeks to conceptualize the subject more clearly, to describe the best available scientific methods that can be used in characterizing and managing environmental health risks, to extend the field of environmental health through new theoretical perspectives and heightened appreciation of social, economic and political contexts, and to encourage a richer analysis in the field through examples of diverse experiences in dealing with the health-environment interface. The Encyclopedia of Environmental Health contains numerous examples of policy options and environmental health practices that have worked and thus can guide programs in other countries or regions It includes a wide range of tools and strategies that can assist communities and countries in assessing environmental health conditions, monitoring progress of intervention implementation and evaluating outcomes Provides a comprehensive overview of existing knowledge in this emerging field Articles contain summaries and assessments of environmental health practices and research, providing a framework for further research Places environmental health in the broader context of environmental change and related ecological, political, economic, social, and cultural issues

Author : Ian Robert Falconer
Publisher : CRC Press
Page : 303 pages
File Size : 49,6 Mb
Release : 2004-12-20
Category : Science
ISBN : 9780203022870

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Cyanobacterial Toxins of Drinking Water Supplies by Ian Robert Falconer Pdf

The contamination of both drinking and recreational water supplies by cyanobacteria is increasingly a cause for concern worldwide. While contamination causes livestock deaths with relative frequency, acute poisoning is rare in humans. However, there is growing apprehension over the possible role of cylindrospermopsins and microcystins in gastrointe

Author : Anonim
Publisher : Unknown
Page : 33 pages
File Size : 54,6 Mb
Release : 2022
Category : Diquat
ISBN : 0660395487

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Guidelines for Canadian Drinking Water Quality by Anonim Pdf