Electrophoretic Techniques

Author : Angela R. Piergiovanni,José Manuel Herrero-Martínez
Publisher : MDPI
Page : 110 pages
File Size : 50,6 Mb
Release : 2019-07-23
Category : Science
ISBN : 9783039212279

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Analysis of Peptides and Proteins by Electrophoretic Techniques by Angela R. Piergiovanni,José Manuel Herrero-Martínez Pdf

The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.

Author : Donald H. Whitmore
Publisher : CRC Press
Page : 366 pages
File Size : 42,7 Mb
Release : 1990-09-24
Category : Science
ISBN : 0849364167

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Electrophoretic and Isoelectric Focusing Techniques in Fisheries Management by Donald H. Whitmore Pdf

Probably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics.

Author : Colin F. Simpson,Mary Whittaker
Publisher : Unknown
Page : 302 pages
File Size : 50,8 Mb
Release : 1983
Category : Science
ISBN : UOM:39015042093107

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Electrophoretic Techniques by Colin F. Simpson,Mary Whittaker Pdf

Author : Ivor Smith
Publisher : Elsevier
Page : 224 pages
File Size : 51,6 Mb
Release : 2013-09-03
Category : Science
ISBN : 9781483222080

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Zone Electrophoresis by Ivor Smith Pdf

Chromatography and Electrophoretic Techniques, Volume II: Zone Electrophoresis presents a number of methods, all based on zone electrophoresis, which has been carried out on commercially available apparatus offering many advantages to the majority of laboratories. This book is composed of six chapters and begins with discussions on the principles, instrumentation, and applications of paper electrophoresis at low voltages, such as voltages not exceeding 300-400 volts or a potential drop of not more than 10 volts/cm in the direction of migration. The next chapter describes the general experimental methods for the separation of abnormal hemoglobins and surveys the application of paper electrophoresis to the final identification of a hemoglobin variant. The remaining chapters deal with the principles, apparatus, reagents, and applications of other zone electrophoretic techniques, including cellulose acetate; agar gel, starch block, starch gel, and sponge rubber; high voltage paper; and continuous electrophoresis. This book will prove useful to analytical chemists and biologists.

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 40,8 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

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Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Author : Anthony T. Andrews
Publisher : Unknown
Page : 476 pages
File Size : 55,5 Mb
Release : 1986
Category : Electrochemistry
ISBN : UCSD:31822002317543

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Electrophoresis by Anthony T. Andrews Pdf

The new edition has been extensively revised and updated, with enlarged sections on silver staining methods, nucleic acid sequencing, computerized data handling, iso-electric focusing, and isotachophoresis.

Author : Ivor Smith,J. W. T. Seakins
Publisher : Unknown
Page : 548 pages
File Size : 47,5 Mb
Release : 1976
Category : Analytical chemistry
ISBN : STANFORD:36105003342305

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Chromatographic and Electrophoretic Techniques by Ivor Smith,J. W. T. Seakins Pdf

Author : J. A. Leenheer,R. L. Malcolm
Publisher : Unknown
Page : 24 pages
File Size : 42,7 Mb
Release : 1973
Category : Electrophoresis
ISBN : IND:30000143974115

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Preparative Free-flow Electrophoresis as a Method of Fractionation of Natural Organic Materials by J. A. Leenheer,R. L. Malcolm Pdf

Author : Reiner Westermeier
Publisher : John Wiley & Sons
Page : 426 pages
File Size : 42,9 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527604449

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Electrophoresis in Practice by Reiner Westermeier Pdf

This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.

Author : J.M. Walker,W. Gaastra
Publisher : Springer Science & Business Media
Page : 304 pages
File Size : 51,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401165631

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Techniques in Molecular Biology by J.M. Walker,W. Gaastra Pdf

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Author : Anonim
Publisher : Elsevier
Page : 582 pages
File Size : 46,6 Mb
Release : 2000-04-01
Category : Science
ISBN : 0080858244

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Fundamentals and Techniques by Anonim Pdf

Fundamentals and Techniques

Author : Mrs. Mitali Yogesh Patil,Mr. Biprojit Paul,Mr. Mohit Chadha,Dr. Saroj Kumar Raul
Publisher : JEC PUBLICATION
Page : 229 pages
File Size : 50,8 Mb
Release : 2024-07-01
Category : Medical
ISBN : 9789358504958

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MODERN PHARMACEUTICAL ANALYTICAL TECHNIQUES by Mrs. Mitali Yogesh Patil,Mr. Biprojit Paul,Mr. Mohit Chadha,Dr. Saroj Kumar Raul Pdf

In the dynamic realm of pharmaceutical sciences, this project explores "Modern Pharmaceutical Analytical Techniques," delving into cutting-edge methodologies crucial for ensuring the quality and efficacy of drugs. From spectroscopy to advanced technologies like metabolomics, each chapter demystifies the application and significance of these techniques. Bridging academia and industry, this work aims to be a practical guide, underlining the realworld implications of these tools. Gratitude is extended to mentors, colleagues, and institutions, as this concise exploration seeks to serve students, researchers, and professionals navigating the ever-evolving landscape of pharmaceutical analysis.

Author : James W. Jorgenson,Marshall Phillips
Publisher : Unknown
Page : 294 pages
File Size : 52,7 Mb
Release : 1987
Category : Science
ISBN : UOM:39015016084124

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New Directions in Electrophoretic Methods by James W. Jorgenson,Marshall Phillips Pdf

Annotation Developed from a symposium at the 190th Meeting of the American Chemical Society, Chicago, Illinois, September 8-13, 1985. Electrophoresis remains the most powerful method available for separating and analyzing complex mixtures of charged biopolymers, playing a key role in the latest advances in medicine, agriculture, chemistry, and biotechnology. This sixteen-chapter volume traces recent developments in electrophoretic separations, technology, and applications. Isotachophoresis, zone electrophoresis, isoelectric focusing, two dimensional electrophoresis, and pulsed electrophoresis are among the methods discussed. Additional topics include applications of electrophoretic methods in agriculture and forensic serology, the development of standards for electrophoresis, and synthetic ion-containing polymers. Annotation(c) 2003 Book News, Inc., Portland, OR (booknews.com).

Author : Ivor Smith
Publisher : Unknown
Page : 546 pages
File Size : 49,6 Mb
Release : 1968
Category : Chromatographic analysis
ISBN : UOM:39015003797324

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Chromatographic and Electrophoretic Techniques: Zone electrophoresis by Ivor Smith Pdf

Author : Grady Hanrahan,Frank A. Gomez
Publisher : John Wiley & Sons
Page : 460 pages
File Size : 42,8 Mb
Release : 2009-10-22
Category : Science
ISBN : 9780470530184

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Chemometric Methods in Capillary Electrophoresis by Grady Hanrahan,Frank A. Gomez Pdf

Use chemometric techniques to develop optimum separation conditions for capillary electrophoreses For all its advantages, capillary electrophoresis (CE) also carries significant disadvantages for the researcher. Offering a unique blend of information from authors active in a variety of developments of chemometrics in CE, Chemometric Methods in Capillary Electrophoresis presents modern chemometric methods as an alternative to help alleviate the problems commonly encountered during routine analysis and method development. Focusing on current chemometric methods utilized in CE endeavours by research-active experts in the field, the book begins with a thorough introduction to CE and chemometric-related concepts and the need for modern chemometric methods in CE. Part 1 discusses differing types of screening designs and response surface methodology¿in an¿application based format Part 2 includes vital discussion on various exploratory data analysis, prediction, and classification techniques utilized in CE-related studies Part 3 provides practical information on modelling quantitative structure relationships Part 4 explores transformation techniques, in particular fundamental studies and applications of cross-correlation and Hadamard Transform Electrophoresis Showing how chemometric methods are applied in a wide array of applications including biological, medical, pharmaceutical, food, forensic, and environmental science, Chemometric Methods in Capillary Electrophoresis is not only highly significant to capillary electrophoresis-based endeavours, but instructive for investigators active in other areas of separation science who could benefit from its informative content.