Techniques In Molecular Biology

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Molecular Biology Techniques

Heather Miller,D. Scott Witherow,Sue Carson
Molecular Biology Techniques Book PDF

Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 48,9 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459

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Molecular Biology Techniques by Heather Miller,D. Scott Witherow,Sue Carson Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Molecular Biology Techniques

Walt Ream,Katharine G. Field
Molecular Biology Techniques Book PDF

Author : Walt Ream,Katharine G. Field
Publisher : Academic Press
Page : 245 pages
File Size : 44,9 Mb
Release : 1998-11-17
Category : Science
ISBN : 9780080536828

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Molecular Biology Techniques by Walt Ream,Katharine G. Field Pdf

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter

Basic Techniques in Molecular Biology

Stefan Surzycki
Basic Techniques in Molecular Biology Book PDF

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
Page : 434 pages
File Size : 41,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642569685

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Basic Techniques in Molecular Biology by Stefan Surzycki Pdf

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Techniques in Molecular Biology

J.M. Walker,W. Gaastra
Techniques in Molecular Biology Book PDF

Author : J.M. Walker,W. Gaastra
Publisher : Springer Science & Business Media
Page : 304 pages
File Size : 45,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401165631

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Techniques in Molecular Biology by J.M. Walker,W. Gaastra Pdf

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Basic Methods in Molecular Biology

Leonard G. Davis,Mark D. Dibner,James F. Battey
Basic Methods in Molecular Biology Book PDF

Author : Leonard G. Davis,Mark D. Dibner,James F. Battey
Publisher : Unknown
Page : 404 pages
File Size : 49,7 Mb
Release : 1992
Category : Molecular biology
ISBN : IND:30000121511939

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Basic Methods in Molecular Biology by Leonard G. Davis,Mark D. Dibner,James F. Battey Pdf

The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another.

Advanced Methods in Molecular Biology and Biotechnology

Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Advanced Methods in Molecular Biology and Biotechnology Book PDF

Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Page : 220 pages
File Size : 48,9 Mb
Release : 2020-10-28
Category : Science
ISBN : 9780128244500

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Advanced Methods in Molecular Biology and Biotechnology by Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool Pdf

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Principles and Techniques of Biochemistry and Molecular Biology

Keith Wilson,John Walker
Principles and Techniques of Biochemistry and Molecular Biology Book PDF

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 48,6 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

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Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Practical Methods in Molecular Biology

Robert F. Schleif,Pieter C. Wensink
Practical Methods in Molecular Biology Book PDF

Author : Robert F. Schleif,Pieter C. Wensink
Publisher : Springer Science & Business Media
Page : 229 pages
File Size : 50,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461259565

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Practical Methods in Molecular Biology by Robert F. Schleif,Pieter C. Wensink Pdf

This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.

Molecular Biology

Susan J. Karcher
Molecular Biology Book PDF

Author : Susan J. Karcher
Publisher : Academic Press
Page : 280 pages
File Size : 48,8 Mb
Release : 1995-11-28
Category : Science
ISBN : 0080536832

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Molecular Biology by Susan J. Karcher Pdf

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)

Techniques in Molecular Medicine

Friedhelm Hildebrandt,Peter Igarashi
Techniques in Molecular Medicine Book PDF

Author : Friedhelm Hildebrandt,Peter Igarashi
Publisher : Springer Science & Business Media
Page : 394 pages
File Size : 52,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783642598111

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Techniques in Molecular Medicine by Friedhelm Hildebrandt,Peter Igarashi Pdf

This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual

Morphology Methods

Ricardo V. Lloyd
Morphology Methods Book PDF

Author : Ricardo V. Lloyd
Publisher : Springer Science & Business Media
Page : 435 pages
File Size : 55,6 Mb
Release : 2001-06-15
Category : Medical
ISBN : 9781592591909

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Morphology Methods by Ricardo V. Lloyd Pdf

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Computational Methods in Molecular Biology

S.L. Salzberg,D.B. Searls,S. Kasif
Computational Methods in Molecular Biology Book PDF

Author : S.L. Salzberg,D.B. Searls,S. Kasif
Publisher : Elsevier
Page : 368 pages
File Size : 55,5 Mb
Release : 1998-06-19
Category : Computers
ISBN : 0080860931

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Computational Methods in Molecular Biology by S.L. Salzberg,D.B. Searls,S. Kasif Pdf

Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Advances in Molecular Techniques

Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
Advances in Molecular Techniques Book PDF

Author : Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
Publisher : CRC Press
Page : 436 pages
File Size : 52,7 Mb
Release : 2018-05-11
Category : Medical
ISBN : 9781351021210

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Advances in Molecular Techniques by Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh Pdf

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Oral Biology

Gregory J. Seymour,Mary P. Cullinan,Nicholas C.K. Heng,Paul R. Cooper
Oral Biology Book PDF

Author : Gregory J. Seymour,Mary P. Cullinan,Nicholas C.K. Heng,Paul R. Cooper
Publisher : Springer Nature
Page : 523 pages
File Size : 50,7 Mb
Release : 2022-11-23
Category : Medical
ISBN : 9781071627808

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Oral Biology by Gregory J. Seymour,Mary P. Cullinan,Nicholas C.K. Heng,Paul R. Cooper Pdf

This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.

Techniques for Molecular Biology

D Tagu
Techniques for Molecular Biology Book PDF

Author : D Tagu
Publisher : CRC Press
Page : 230 pages
File Size : 41,8 Mb
Release : 2006-01-04
Category : Science
ISBN : 9781482294460

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Techniques for Molecular Biology by D Tagu Pdf

This book explains the theoretical principles of numerous techniques of genomic studies developed recently in laboratories. The book is designed for any reader who is curious to know the bases of different techniques of manipulation of nucleic acids.