Encyclopaedia Of Analytical Techniques In Biochemistry And Molecular Biology

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Analytical Techniques in Biochemistry and Molecular Biology

Author : Rajan Katoch
Publisher : Springer Science & Business Media
Page : 441 pages
File Size : 49,7 Mb
Release : 2011-07-19
Category : Science
ISBN : 1441997857

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Analytical Techniques in Biochemistry and Molecular Biology by Rajan Katoch Pdf

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Bioanalytics

Author : Friedrich Lottspeich,Joachim W. Engels
Publisher : John Wiley & Sons
Page : 1151 pages
File Size : 48,7 Mb
Release : 2018-05-29
Category : Science
ISBN : 9783527339198

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Bioanalytics by Friedrich Lottspeich,Joachim W. Engels Pdf

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Analytical Techniques in Biosciences

Author : Chukwuebuka Egbuna,Kingsley C. Patrick-Iwuanyanwu,Muhammad Ajmal Shah,Jonathan C. Ifemeje,Azhar Rasul
Publisher : Academic Press
Page : 302 pages
File Size : 52,7 Mb
Release : 2021-10-21
Category : Science
ISBN : 9780128227992

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Analytical Techniques in Biosciences by Chukwuebuka Egbuna,Kingsley C. Patrick-Iwuanyanwu,Muhammad Ajmal Shah,Jonathan C. Ifemeje,Azhar Rasul Pdf

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences

Principles of Analytical Biochemistry

Author : Thomas Rutherford
Publisher : Unknown
Page : 128 pages
File Size : 53,5 Mb
Release : 2019-08-23
Category : Electronic
ISBN : 1645320294

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Principles of Analytical Biochemistry by Thomas Rutherford Pdf

Analytical techniques such as spectroscopy, chromatography, etc. are particularly important in analytical biochemistry as well as in analytical chemistry generally. The principles of each technique are explained and the scope and applications are discussed. There are chapters on enzymes, antibodies and radio-isotopes, substances which it may be necessary to detect and measure but which also can be very useful in a variety of analytical methods. The present title "Principles of Analytical Biochemistry" is intended for those who wish to understand living organisms, especially man. Biochemistry is essential for this purpose, but it would be almost impossible for a student to survey on his own the massive body of existing knowledge, constantly augmented by a remarkable torrent of brilliant discoveries. Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions. Most people consider biochemistry to be synonymous with molecular biology. Biochemistry today has made spectacular progress in unraveling the mysteries of animate nature. This progress has allowed us to gain deeper insight into the principles of vital activity and has to a very significant extent stimulated the development of applied disciplines, especially medicine. The aim of this book is to present a core of biochemical knowledge that is desirable for undergraduate and postgraduate students and also those involved in the field of medical, microbiology, biotechnology and pharmaceutical.

Reviews in Cell Biology and Molecular Medicine

Author : Robert A. Meyers
Publisher : Wiley-Blackwell
Page : 0 pages
File Size : 43,5 Mb
Release : 2008-04-29
Category : Science
ISBN : 3527305424

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Reviews in Cell Biology and Molecular Medicine by Robert A. Meyers Pdf

"This series is a classic..." - Molecular Medicine Today/Trends in Molecular Medicine The second edition of this highly acclaimed, sixteen-volume Encyclopedia now contains 150 new articles and extended coverage of cell biology. It is thus the most comprehensive and most detailed treatment of molecular biology, cell biology and molecular medicine available today -- designed in collaboration with a founding board of 10 Nobel laureates. As such, the Encyclopedia provides a single-source library of the molecular basis of life, with a focus on molecular medicine, discussing in detail the latest advances of the post-genomic era. Each of the approximately 425 articles is written as a self-contained treatment, beginning with an outline and a key word section plus definitions. Peer-reviewed, they are written in a review-like style, complemented by an extensive bipartite bibliography of reviews and books as well as primary papers. A glossary of basic terms completes each volume and defines the most commonly used terms in molecular biology. Together with the introductory illustrations found in each volume, the articles are comprehensible for readers at every level without resorting to a dictionary, textbook, or other reference. Praise for the first edition: "...an authoritative reference source of the highest quality. ... It is extremely well written and well illustrated..." - American Reference Books Annual (Library & Information Science Annual) "This series can be recommended without hesitation to a broad readership including students and qualified researchers... . ...articles...set-up facilitates easy reading and rapid understanding. ...overwhelming amount of valuable data." - Molecular Biology Reports ".. highly valuable and recommendable both for libraries and for laboratory use." - FEBS Letters

Analytical Molecular Biology

Author : Helen Parkes,V (Ginny) Saunders
Publisher : Royal Society of Chemistry
Page : 209 pages
File Size : 51,6 Mb
Release : 2007-10-31
Category : Science
ISBN : 9781847559296

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Analytical Molecular Biology by Helen Parkes,V (Ginny) Saunders Pdf

In spite of the wide variety and complexity of biological materials, nucleic acids are ubiquitous. DNA is becoming the bioanalyte of choice due to the vast amount of information embedded in its sequence, its robust chemical nature and the range of highly sensitive analytical techniques that have been developed. The results of such analyses can have an important impact on our society both commercially and in terms of the quality of life. Absolute confidence in the data generated is therefore of the utmost importance. This book, produced by LGC as part of the VAM (Valid Analytical Measurement) Programme, introduces the issues of validation and quality to the bioanalytical community, specifically addressing DNA-based analyses. It aims to raise awareness of the factors that can influence the validity of DNA analysis and the production of quality data. Emphasis is placed on VAM principles, as well as additional challenges that are associated with the analysis of real samples, for example, complex food matrices or forensic samples that have been subjected to environmental insult. Information is collated from a variety of sources including literature, discussions and LGC research, and offers constructive advice where possible.

Encyclopedia of Lipidomics

Author : Markus R. Wenk
Publisher : Springer
Page : 0 pages
File Size : 43,9 Mb
Release : 2018-07-11
Category : Medical
ISBN : 9400774664

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Encyclopedia of Lipidomics by Markus R. Wenk Pdf

The Encyclopedia of Lipidomics will present a complete overview of the field from fundamentals to new discoveries and from concepts, theories and experimental techniques to clinical and industrial applications. The book will develop with the active involvement of a strong editorial board comprised of leaders from the field. The Encyclopedia of Lipidomics intends to be a comprehensive reference resource serving to bridge the gap between clinical and basic science investigators and provide authoritative and digested information to students, scientists as well as non specialists. The book will have an edge over protocol type of works and databases in terms of having wider coverage through rigorous essays on terms, concepts, experimental and analytical techniques, applications and new challenges. Since lipidomics has strong linkages with several of the other biomedical and life sciences disciplines, the proposed encyclopedia will have a wide audience including graduate students, researchers and different levels of scientists in biomedicine, cellular and molecular biology, bioengineering, physiological and biochemistry, and pharmacology across both academia and industry.

Handbook of Analysis of Oligonucleotides and Related Products

Author : Jose V. Bonilla,G. Susan Srivatsa
Publisher : CRC Press
Page : 514 pages
File Size : 46,7 Mb
Release : 2011-02-23
Category : Medical
ISBN : 9781439819937

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Handbook of Analysis of Oligonucleotides and Related Products by Jose V. Bonilla,G. Susan Srivatsa Pdf

Oligonucleotides represent one of the most significant pharmaceutical breakthroughs in recent years, showing great promise as diagnostic and therapeutic agents for malignant tumors, cardiovascular disease, diabetes, viral infections, and many other degenerative disorders. The Handbook of Analysis of Oligonucleotides and Related Products is an essential reference manual on the practical application of modern and emerging analytical techniques for the analysis of this unique class of compounds. A strong collaboration among thirty leading analytical scientists from around the world, the book provides readers with a comprehensive overview of the most commonly used analytical techniques and their advantages and limitations in assuring the identity, purity, quality, and strength of an oligonucleotide intended for therapeutic use. Topics discussed include: Strategies for enzymatic or chemical degradation of chemically modified oligonucleotides toward mass spectrometric sequencing Purity analysis by chromatographic or electrophoretic methods, including RP-HPLC, AX-HPLC, HILIC, SEC, and CGE Characterization of sequence-related impurities in oligonucleotides by mass spectrometry and chromatography Structure elucidation by spectroscopic methods (IR, NMR, MS) as well as base composition and thermal melt analysis (Tm) Approaches for the accurate determination of molar extinction coefficient of oligonucleotides Accurate determination of assay values Assessment of the overall quality of oligonucleotides, including microbial analysis and determination of residual solvents and heavy metals Strategies for determining the chemical stability of oligonucleotides The use of hybridization techniques for supporting pharmacokinetics and drug metabolism studies in preclinical and clinical development Guidance for the presentation of relevant analytical information towards meeting current regulatory expectations for oligonucleotide therapeutics This resource provides a practical guide for applying state-of-the-art analytical techniques in research, development, and manufacturing settings.

Molecular Biology and Biotechnology

Author : Robert Allen Meyers
Publisher : John Wiley & Sons
Page : 1090 pages
File Size : 53,9 Mb
Release : 1995-06-29
Category : Medical
ISBN : 0471186341

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Molecular Biology and Biotechnology by Robert Allen Meyers Pdf

This is one volume 'library' of information on molecular biology, molecular medicine, and the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. The purpose is to assist the expanding number of scientists entering molecular biology research and biotechnology applications from diverse backgrounds, including biology and medicine, as well as physics, chemistry, mathematics, and engineering.

Encyclopedia of Molecular Cell Biology and Molecular Medicine

Author : Robert A. Meyers
Publisher : Wiley-Blackwell
Page : 696 pages
File Size : 45,8 Mb
Release : 2004-04-16
Category : Science
ISBN : 3527305440

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Encyclopedia of Molecular Cell Biology and Molecular Medicine by Robert A. Meyers Pdf

This sixteen volume encyclopedia is the most comprehensive and detailed treatment of molecular biology, cell biology and molecular medicine available today! It was designed in collaboration with a founding board of 10 Nobel laureates. The Encyclopedia provides a single-source library of the molecular basis of life, with a focus on molecular medicine. The latest advances of the post-genomic era, e.g. in the fields of functional genomics, proteomics, and bioinformatics are discussed in detail. All articles are designed as self-contained treatments. Each of the approximately 425 articles begins with an outline and a key word section with definitions. Articles are written in a review-like style complemented with an extensive bipartite bibliography of reviews and books as well as primary papers. A glossary of basic terms completes each volume and defines the most commonly used terms in molecular biology. Together with the introductory illustrations found in each volume, the articles enable readers to understand articles without referring to a dictionary, textbook, or other reference. Praise for the first edition of the preceding "Encyclopedia of Molecular Biology and Molecular Medicine": "...an authoritative reference source of the highest quality. ... It is extremely well written and well illustrated..." - American Reference Books Annual (Library & Information Science Annual) "This series can be recommended without hesitation to a broad readership including students and qualified researchers... . ...articles...set-up facilitates easy reading and rapid understanding. ...overwhelming amount of valuable data." - Molecular Biology Reports ".. highly valuable and recommendable both for libraries and for laboratory use." - FEBS Letters "This series is a classic..." - Molecular Medicine Today/Trends in Molecular Medicine

Analytical Techniques in Biochemistry

Author : Mahin Basha
Publisher : Unknown
Page : 126 pages
File Size : 41,5 Mb
Release : 2020
Category : Analytical biochemistry
ISBN : 1071601342

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Analytical Techniques in Biochemistry by Mahin Basha Pdf

This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications. The contributions, which each focus on a specific technique, are based on a thorough review and analysis of the current literature as well as the authors' experiences in the lab. Divided into nine parts, the book provides insights into basic separation techniques like sedimentation, filtration and centrifugation, as well as analytical techniques such as spectrophotometry, chromatography, electrophoresis, immuno-techniques, radioactivity and microscopy. .

Encyclopedia of Molecular Cell Biology and Molecular Medicine

Author : Robert A. Meyers
Publisher : Wiley-Blackwell
Page : 720 pages
File Size : 50,8 Mb
Release : 2004-04-16
Category : Science
ISBN : 3527305459

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Encyclopedia of Molecular Cell Biology and Molecular Medicine by Robert A. Meyers Pdf

This sixteen volume encyclopedia is the most comprehensive and detailed treatment of molecular biology, cell biology and molecular medicine available today! It was designed in collaboration with a founding board of 10 Nobel laureates. The Encyclopedia provides a single-source library of the molecular basis of life, with a focus on molecular medicine. The latest advances of the post-genomic era, e.g. in the fields of functional genomics, proteomics, and bioinformatics are discussed in detail. All articles are designed as self-contained treatments. Each of the approximately 425 articles begins with an outline and a key word section with definitions. Articles are written in a review-like style complemented with an extensive bipartite bibliography of reviews and books as well as primary papers. A glossary of basic terms completes each volume and defines the most commonly used terms in molecular biology. Together with the introductory illustrations found in each volume, the articles enable readers to understand articles without referring to a dictionary, textbook, or other reference. Praise for the first edition of the preceding "Encyclopedia of Molecular Biology and Molecular Medicine": "...an authoritative reference source of the highest quality. ... It is extremely well written and well illustrated..." - American Reference Books Annual (Library & Information Science Annual) "This series can be recommended without hesitation to a broad readership including students and qualified researchers... . ...articles...set-up facilitates easy reading and rapid understanding. ...overwhelming amount of valuable data." - Molecular Biology Reports ".. highly valuable and recommendable both for libraries and for laboratory use." - FEBS Letters "This series is a classic..." - Molecular Medicine Today/Trends in Molecular Medicine

Encyclopedia of Molecular Cell Biology and Molecular Medicine, Syngamy and Cell Cycle Control to Triacylglyerol Storage and Mobilization, Regulation of

Author : Robert A. Meyers
Publisher : Wiley-Blackwell
Page : 766 pages
File Size : 55,6 Mb
Release : 2005-10-28
Category : Science
ISBN : 352730651X

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Encyclopedia of Molecular Cell Biology and Molecular Medicine, Syngamy and Cell Cycle Control to Triacylglyerol Storage and Mobilization, Regulation of by Robert A. Meyers Pdf

This second edition of the Encyclopedia of Molecular Cell Biology and Molecular Medicine covers the molecular and cellular basis of life, disease, and therapy at university and professional researcher level. With its 16 volumes, this is the most comprehensive and detailed treatment of molecular cell biology and molecular medicine available today. It represents a single source library for Molecular Biologists Cell Biologist Biochemists Structural Biologists Gene Technologists Developmental Biologists Medicinal Chemists Physicians Biotechnologists Pharmacologists An Editorial Board composed of renowned experts from all over the world – Nobel laureates, including the 2007 Nobel Prize winner in medicine, Sir Martin Evans, Lasker Award winners and directors of prestigious institutes and university departments – guarantees the high quality and comprehensive scope of this work. All major disciplines comprising and supporting molecular cell biology and molecular medicine are covered in true Encyclopedic detail. Each of the over 400 articles is conceived as a self-contained treatment and begins with an outline and a keyword section, including definitions. Descriptive illustrations – many in colour -, informative tables and a glossary of basic terms in each volume enable readers to understand articles without the need to consult a dictionary, textbook or other work. Numerous cross-references and a comprehensive bibliography round off every article. Praise from the reviews: "... It goes without saying that no library can afford to be without this new edition. Everyone working in the areas of molecular biology, genome research, medical science, or clinical research needs to have access to these volumes... " Angewandte Chemie "... an authoritative reference source of the highest quality… It is extremely well written and well illustrated..."American Reference Books Annual (Library & Information Science Annual - on the first edition) For further details please visit our homepage at www.meyers-emcbmm.de