Epigenetics Protocols

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Epigenetics Protocols

Trygve O. Tollefsbol
Epigenetics Protocols Book PDF

Author : Trygve O. Tollefsbol
Publisher : Springer Science & Business Media
Page : 301 pages
File Size : 45,9 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592598281

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Epigenetics Protocols by Trygve O. Tollefsbol Pdf

The field of epigenetics has grown exponentially in the past decade, and a steady flow of exciting discoveries in this area has served to move it to the forefront of molecular biology. Although epigenetics may previously have been considered a peripheral science, recent advances have shown considerable progress in unraveling the many mysteries of nontraditional genetic processes. Given the fast pace of epigenetic discoveries and the groundbreaking nature of these developments, a thorough treatment of the methods in the area seems timely and appropriate and is the goal of Epigenetics Protocols. The scope of epigenetics is vast, and an exhaustive analysis of all of the techniques employed by investigators would be unrealistic. However, this TM volume of Methods in Molecular Biology covers three main areas that should be of greatest interest to epigenetics investigators: (1) techniques related to analysis of chromatin remodeling, such as histone acetylation and methylation; (2) methods in newly developed and especially promising areas of epigenetics such as telomere position effects, quantitative epigenetics, and ADP ribosylation; and (3) an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure.

Population Epigenetics

Paul Haggarty,Kristina Harrison
Population Epigenetics Book PDF

Author : Paul Haggarty,Kristina Harrison
Publisher : Humana
Page : 0 pages
File Size : 53,7 Mb
Release : 2017-02-17
Category : Medical
ISBN : 1493969013

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Population Epigenetics by Paul Haggarty,Kristina Harrison Pdf

This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. /divAuthoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.

Epigenetics Methods

Trygve Tollefsbol
Epigenetics Methods Book PDF

Author : Trygve Tollefsbol
Publisher : Academic Press
Page : 736 pages
File Size : 40,6 Mb
Release : 2020-07-08
Category : Medical
ISBN : 9780128194157

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Epigenetics Methods by Trygve Tollefsbol Pdf

In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods Written at verbal and technical levels that can be understood by scientists and students alike Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics

Plant Epigenetics and Epigenomics

Charles Spillane,Peter C. McKeown
Plant Epigenetics and Epigenomics Book PDF

Author : Charles Spillane,Peter C. McKeown
Publisher : Humana
Page : 0 pages
File Size : 52,7 Mb
Release : 2016-08-27
Category : Science
ISBN : 1493962299

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Plant Epigenetics and Epigenomics by Charles Spillane,Peter C. McKeown Pdf

Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape. These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seek to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.

Plant Epigenetics

Igor Kovalchuk,Franz J. Zemp
Plant Epigenetics Book PDF

Author : Igor Kovalchuk,Franz J. Zemp
Publisher : Humana Press
Page : 273 pages
File Size : 50,7 Mb
Release : 2011-08-24
Category : Science
ISBN : 1607616505

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Plant Epigenetics by Igor Kovalchuk,Franz J. Zemp Pdf

The discovery of DNA as the genetic material brought great hope to scientists all over the world. It was believed that many of the lingering questions in genetics and the mechanisms of heredity would fnally be answered. However, as often is the case in science, more qu- tions arose out of this discovery. What defnes a gene? What are the mechanisms of gene regulation? Further discovery and technological innovations brought about sequencing techniques that allowed the study of complete genomes from many organisms, including Arabidopsis and humans. Despite all the excitement surrounding these technologies, many features of the genome remained unclear. Peculiar characteristics in genome composition such as signifcant redundancy consisting of many repetitive elements and noncoding sequences, active transcriptional units with no protein product, and unusual sequences in promoter regions added to the mysteries of genetic make-up and gene regulation. Indeed, the more we discovered about the genome, the more diffcult it became to understand the complexity of cellular function and regulation. Out of the study of the intricacies of the genome and gene regulation, arose a new science that was independent of actual DNA changes, but critical in maintaining gene regulation and genetic stability. Epigenetics, literally translated as “above genetics,” is the science that describes the mechanisms of heritable changes in gene regulation that does not involve modifcations of DNA sequence. These changes may last through somatic cell division and, in some cases, throughout multiple generations.

Epigenomics

Izuho Hatada,Takuro Horii
Epigenomics Book PDF

Author : Izuho Hatada,Takuro Horii
Publisher : Springer Nature
Page : 292 pages
File Size : 49,7 Mb
Release : 2022-09-29
Category : Science
ISBN : 9781071627242

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Epigenomics by Izuho Hatada,Takuro Horii Pdf

This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.

Cancer Epigenetics for Precision Medicine

Ramona G. Dumitrescu,Mukesh Verma
Cancer Epigenetics for Precision Medicine Book PDF

Author : Ramona G. Dumitrescu,Mukesh Verma
Publisher : Humana
Page : 298 pages
File Size : 49,7 Mb
Release : 2019-09-17
Category : Medical
ISBN : 1493993836

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Cancer Epigenetics for Precision Medicine by Ramona G. Dumitrescu,Mukesh Verma Pdf

This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more. The chapters in this book further delve into this field and cover topics such as epigenome-based precision medicine in lung cancer; interplay between genetic and epigenetic changes in breast cancer subtypes; genetic regulation of PDCD1 gene in cancer immunology; and pyrosequencing methylation analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Cancer Epigenetics for Precision Medicine: Methods and Protocols is a valuable resource to help researchers and scientists identify these specific biomarkers and work towards the prevention, diagnosis, and prognosis of different cancers in the future.

Cancer Epigenetics

Ramona G. Dumitrescu,Mukesh Verma
Cancer Epigenetics Book PDF

Author : Ramona G. Dumitrescu,Mukesh Verma
Publisher : Humana Press
Page : 492 pages
File Size : 51,5 Mb
Release : 2012-02-24
Category : Medical
ISBN : 1617796115

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Cancer Epigenetics by Ramona G. Dumitrescu,Mukesh Verma Pdf

The epigenetic regulation plays an important role in normal development and maintenance of tissue specific genes expression in humans and the disturbance of these patterns lead to changes involved in tumor formation. More recently, epigenetic changes have been observed in early stages of tumor development and together with the genetic alterations have been defined as abnormalities, necessary for cancer initiation and progression. In, Cancer Epigenetics: Methods and Protocols, expert researchers reviewed these epigenetics changes in different tumor types and described several technologies that are currently available to detect epigenetic changes. These technologies have lead to a better understanding of the processes in normal and cancerous cells. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Cancer Epigenetics: Methods and Protocols aids scientists in continuing to study epigenetic alterations used in clinical practice as biomarkers of early cancerous lesions or markers of progression and prognosis.

Epigenetic Reprogramming During Mouse Embryogenesis

Katia Ancelin,Maud Borensztein
Epigenetic Reprogramming During Mouse Embryogenesis Book PDF

Author : Katia Ancelin,Maud Borensztein
Publisher : Humana
Page : 0 pages
File Size : 49,9 Mb
Release : 2020-09-18
Category : Science
ISBN : 1071609602

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Epigenetic Reprogramming During Mouse Embryogenesis by Katia Ancelin,Maud Borensztein Pdf

This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming.

Epigenome Editing

Albert Jeltsch,Marianne G. Rots
Epigenome Editing Book PDF

Author : Albert Jeltsch,Marianne G. Rots
Publisher : Unknown
Page : 482 pages
File Size : 47,9 Mb
Release : 2018
Category : Epigenetics
ISBN : 1493977741

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Epigenome Editing by Albert Jeltsch,Marianne G. Rots Pdf

This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.

Handbook of Epigenetics

Trygve O Tollefsbol
Handbook of Epigenetics Book PDF

Author : Trygve O Tollefsbol
Publisher : Academic Press
Page : 920 pages
File Size : 51,5 Mb
Release : 2022-09-08
Category : Science
ISBN : 9780323919500

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Handbook of Epigenetics by Trygve O Tollefsbol Pdf

Handbook of Epigenetics: The New Molecular and Medical Genetics, Third Edition provides a comprehensive analysis of epigenetics, from basic biology to clinical application. This new edition has been fully revised to cover the latest and evolving topics in epigenetics, with chapters updated and new chapters added on topics such as single-cell epigenetics, DNA methylation clocks in age-related diseases, transposable elements and epigenetics, X chromosome inactivation, and the epigenetics of drug addiction, among other topics. Throughout this edition, greater emphasis falls on epigenomic analyses and incorporating multi-omics approaches rather than gene-specific analyses. In addition, this edition has also been enhanced with step-by-step instructions in research methods, as well as easy-to-digest disease case studies and clinical trials that provide context and applied examples of recent advances in disease understanding and epigenetic therapeutics. These features empower researchers to reproduce the approaches and studies discussed and aid clinical translation. Live links across chapters tie in relevant external datasets and resources. Provides a timely and comprehensive collection of fully up-to-date coverage of epigenetics Covers basic epigenetic biology, research methods and technology, disease relationships and clinical medicine Written at a verbal and technical level that can be understood by scientists and students alike, with chapter summaries and conclusions included throughout Discusses exciting new topics in epigenetics, such as DNA methylation clocks in age-related diseases, transposable elements and epigenetics, X chromosome inactivation, and the epigenetics of drug addiction Includes step-by-step instructions in research protocols to aid reproducibility, as well as easy-to-digest disease case studies and clinical trials, providing context and applied examples of recent clinical translation

Epigenome Editing

Albert Jeltsch,Marianne G. Rots
Epigenome Editing Book PDF

Author : Albert Jeltsch,Marianne G. Rots
Publisher : Humana Press
Page : 482 pages
File Size : 45,9 Mb
Release : 2018-03-10
Category : Medical
ISBN : 1493977733

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Epigenome Editing by Albert Jeltsch,Marianne G. Rots Pdf

This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.

Toxicoepigenetics

Shaun D. McCullough,Dana Dolinoy
Toxicoepigenetics Book PDF

Author : Shaun D. McCullough,Dana Dolinoy
Publisher : Academic Press
Page : 428 pages
File Size : 40,7 Mb
Release : 2018-11-02
Category : Medical
ISBN : 9780128124345

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Toxicoepigenetics by Shaun D. McCullough,Dana Dolinoy Pdf

Toxicoepigenetics: Core Principles and Applications examines the core aspects of epigenetics, including chromatin biology, DNA methylation, and non-coding RNA, as well as fundamental techniques and considerations for studying each of these mechanisms of epigenetic regulation. Although its integration into the field of toxicology is in its infancy, epigenetics have taken center stage in the study of diseases such as cancer, diabetes, and neurodegeneration. Increasing the presence of epigenetics in toxicological research allows for a more in-depth understanding of important aspects of toxicology such as the role of the environment and lifestyle influencing the individual susceptibility to these effects and the trans-generational transmission of these health effects and susceptibilities. Methods chapters are included to help improve efficacy and efficiency of protocols in both the laboratory and the classroom. Toxicoepigenetics: Core Principles and Applications is an essential book for researchers and academics using epigenetics in toxicology research and study. Introduces the fundamental principles and practices for understanding the role of the epigenome in toxicology Presents the foundation of epigenetics for toxicologists with a broad range of backgrounds Discusses the incorporation of epigenetics and epigenomics into current toxicological studies and interpretation of epigenetic data in toxicological applications

Epigenetics

David Martin
Epigenetics Book PDF

Author : David Martin
Publisher : Unknown
Page : 300 pages
File Size : 44,8 Mb
Release : 2002
Category : Electronic
ISBN : 0896039374

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Epigenetics by David Martin Pdf

Epigenetics concerns phenotypic alterations that lack a genetic basis, but generally any phenomenon with a clearly understood molecular basis has taken it's own name and has been excluded from the field. The text provides protocols for the most widely used techniques in the field, as well as a selection of methods focussed on particular systems or problems. These protocols will be useful primarily to basic scientists, but epigenetics has begun to have impact on medicine, and this trend will undoubtedlt be accented in years to come. This volume shows how epigenetic factors may extensively affect disease processing and how understanding of these factors may improve our ability to assess disease risk..

DNA Methylation Protocols

Jörg Tost
DNA Methylation Protocols Book PDF

Author : Jörg Tost
Publisher : Humana Press
Page : 704 pages
File Size : 42,9 Mb
Release : 2018-08-30
Category : Medical
ISBN : 1493984993

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DNA Methylation Protocols by Jörg Tost Pdf

This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.