Experiments In Plant Tissue Culture

Author : John H. Dodds,Lorin W. Roberts
Publisher : International Potato Center
Page : 260 pages
File Size : 55,5 Mb
Release : 1985-10-31
Category : Science
ISBN : 0521315166

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Experiments in Plant Tissue Culture by John H. Dodds,Lorin W. Roberts Pdf

The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Author : Roberta H. Smith
Publisher : Gulf Professional Publishing
Page : 260 pages
File Size : 47,6 Mb
Release : 2000
Category : Nature
ISBN : 0126503427

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Plant Tissue Culture by Roberta H. Smith Pdf

This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media.

Author : John H. Dodds,Lorin W. Roberts
Publisher : Cambridge University Press
Page : 280 pages
File Size : 43,9 Mb
Release : 1995-01-27
Category : Science
ISBN : 0521478928

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Experiments in Plant Tissue Culture by John H. Dodds,Lorin W. Roberts Pdf

This new edition of a highly successful book has been completely revised and updated, and features new illustrations and experiments.

Author : Roberta H. Smith
Publisher : Academic Press
Page : 206 pages
File Size : 51,6 Mb
Release : 2013
Category : Science
ISBN : 9780124159204

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Plant Tissue Culture by Roberta H. Smith Pdf

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Author : John H. Dodds
Publisher : Unknown
Page : 256 pages
File Size : 44,8 Mb
Release : 1995
Category : Plant tissue culture
ISBN : OCLC:1319328317

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Experiments in Plant Tissue Culture by John H. Dodds Pdf

Author : Robert H. Smith
Publisher : Academic Press
Page : 190 pages
File Size : 50,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323160476

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Plant Tissue Culture by Robert H. Smith Pdf

Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Author : Sunghun Park
Publisher : Academic Press
Page : 254 pages
File Size : 41,6 Mb
Release : 2021-02-17
Category : Science
ISBN : 9780323851374

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Plant Tissue Culture by Sunghun Park Pdf

Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012

Author : Robert N. Trigiano
Publisher : Routledge
Page : 470 pages
File Size : 40,5 Mb
Release : 2018-04-27
Category : Science
ISBN : 9781351424035

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Plant Tissue Culture Concepts and Laboratory Exercises by Robert N. Trigiano Pdf

Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Author : Roberta H. Smith
Publisher : Unknown
Page : 188 pages
File Size : 42,7 Mb
Release : 2012-08-03
Category : Plant tissue culture
ISBN : 1974804194

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Plant Tissue Culture by Roberta H. Smith Pdf

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature.

Author : B. N. Sathyanarayana,Dalia B. Varghese
Publisher : I. K. International Pvt Ltd
Page : 299 pages
File Size : 45,6 Mb
Release : 2007
Category : Botany, Experimental
ISBN : 9788189866112

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Plant Tissue Culture by B. N. Sathyanarayana,Dalia B. Varghese Pdf

Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Author : Robert N. Trigiano,Dennis J. Gray
Publisher : CRC Press
Page : 608 pages
File Size : 52,6 Mb
Release : 2016-03-30
Category : Medical
ISBN : 9781439896143

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Plant Tissue Culture, Development, and Biotechnology by Robert N. Trigiano,Dennis J. Gray Pdf

Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol

Author : Roberta H. Smith
Publisher : Unknown
Page : 231 pages
File Size : 47,5 Mb
Release : 2005-01-01
Category : Plant tissue culture
ISBN : 8181475860

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Plant Tissue Culture-Techniques & Experiments, 2E by Roberta H. Smith Pdf

Author : M. K. Razdan
Publisher : Science Publishers
Page : 394 pages
File Size : 46,7 Mb
Release : 2003
Category : Science
ISBN : 1578082374

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Introduction to Plant Tissue Culture by M. K. Razdan Pdf

Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.

Author : J. Reinert,M.M. Yeoman
Publisher : Springer Science & Business Media
Page : 85 pages
File Size : 46,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642817847

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Plant Cell and Tissue Culture by J. Reinert,M.M. Yeoman Pdf

The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

Author : Philip Rodney White
Publisher : Unknown
Page : 304 pages
File Size : 50,9 Mb
Release : 1943
Category : Plant cells and tissues
ISBN : UOM:39015004615640

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A Handbook of Plant Tissue Culture by Philip Rodney White Pdf

The history of plant tissue culture. The living materials. The laboratory. Nutrients. How cultures are started. Culture techniques. Growth measurements and their interpretation. Tissue culture and the study of problems in the pathology and general physiology of plants. Morphogenesis.