Plant Tissue Culture Concepts And Laboratory Exercises

Author : Robert N. Trigiano
Publisher : Routledge
Page : 470 pages
File Size : 43,7 Mb
Release : 2018-04-27
Category : Science
ISBN : 9781351424035

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Plant Tissue Culture Concepts and Laboratory Exercises by Robert N. Trigiano Pdf

Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Author : Robert N. Trigiano
Publisher : Unknown
Page : 128 pages
File Size : 55,8 Mb
Release : 2017
Category : Electronic books
ISBN : 020374313X

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Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition by Robert N. Trigiano Pdf

"Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science."--Provided by publisher.

Author : Caula A. Beyl,Robert N. Trigiano
Publisher : CRC Press
Page : 520 pages
File Size : 44,7 Mb
Release : 2016-01-06
Category : Science
ISBN : 9781466503885

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Plant Propagation Concepts and Laboratory Exercises by Caula A. Beyl,Robert N. Trigiano Pdf

Includes a DVD Containing All Figures and Supplemental Images in PowerPoint This new edition of Plant Propagation Concepts and Laboratory Exercises presents a robust view of modern plant propagation practices such as vegetable grafting and micropropagation. Along with foundation knowledge in anatomy and plant physiology, the book takes a look into the future and how cutting edge research may impact plant propagation practices. The book emphasizes the principles of plant propagation applied in both temperate and tropical environments. In addition to presenting the fundamentals, the book features protocols and practices that students can apply in both laboratory and field experiences. The book shows readers how to choose the best methods for plant propagation including proper media and containers as well as performing techniques such as budding, cutting, layering, grafting, and cloning. It also discusses how to recognize and cope with various propagation challenges. Also included are concept chapters highlighting key information, laboratory exercises, anticipated laboratory results, stimulating questions, and a DVD containing all the figures in the book as well as some supplemental images.

Author : Roberta H. Smith
Publisher : Gulf Professional Publishing
Page : 260 pages
File Size : 43,6 Mb
Release : 2000
Category : Plant cell culture
ISBN : 0126503427

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Plant Tissue Culture by Roberta H. Smith Pdf

This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media.

Author : Robert N. Trigiano,Dennis J. Gray
Publisher : CRC Press
Page : 608 pages
File Size : 47,9 Mb
Release : 2016-03-30
Category : Medical
ISBN : 9781439896143

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Plant Tissue Culture, Development, and Biotechnology by Robert N. Trigiano,Dennis J. Gray Pdf

Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol

Author : Robert H. Smith
Publisher : Academic Press
Page : 190 pages
File Size : 43,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323160476

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Plant Tissue Culture by Robert H. Smith Pdf

Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Author : Robert N. Trigiano
Publisher : CRC Press
Page : 578 pages
File Size : 52,8 Mb
Release : 2007-11-30
Category : Science
ISBN : 9781482226935

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Plant Pathology Concepts and Laboratory Exercises by Robert N. Trigiano Pdf

Revised and updated with new concepts, case studies, and laboratory exercises, Plant Pathology Concepts and Laboratory Exercises, Second Edition supplies highly detailed and accurate information in a well-organized and accessible format. New additions to the second edition include five new topic and exercise chapters on soilborne pathogens, molecular tools, biocontrol, and plant-fungal interactions, information on in vitro pathology, an appendix on plant pathology careers, and how to use and care for the microscope. An accompanying cd-rom contains figures from the text as well as supplemental full-color photos and PowerPoint slides. Unique Learning Tools Retaining the informal style of the previous edition, this volume begins each topic with a concept box to highlight important ideas. Several laboratory exercises support each topic and cater to a wide range of skill sets from basic to complex. Procedure boxes for the experimental exercises give detailed outlines and comments on the experiments, step by step instruction, anticipated results, and thought provoking questions. Case studies of specific diseases and processes are presented as a bulleted list supplying essential information at a glance. Comprehensive Coverage Divided into six primary parts, this valuable reference introduces basic concepts of plant pathology with historical perspectives, fundamental ideas of disease, and disease relationships with the environment. It details various disease-causing organisms including viruses, prokaryotic organisms, plant parasitic nematodes, fungi, plant parasitic seed plants, and other biotic and abiotic diseases. Exploring various plant-pathogen interactions including treatments of molecular attack strategies, extracellular enzymes, host defenses, and disruption of plant function, the book presents the basic ideas of epidemiology, control strategies, and disease diagnosis.

Author : Robert N. Trigiano,Mark Townsend Windham,Alan S. Windham
Publisher : CRC Press
Page : 584 pages
File Size : 49,6 Mb
Release : 2008
Category : Science
ISBN : STANFORD:36105124027736

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Plant Pathology Concepts and Laboratory Exercises, Second Edition by Robert N. Trigiano,Mark Townsend Windham,Alan S. Windham Pdf

This title serves as a resource for both students and researchers, explaining definitions of plant disease, characteristics of organisms that cause disease, and how diseases interact with hosts and the environment.

Author : J. Reinert,M.M. Yeoman
Publisher : Springer Science & Business Media
Page : 85 pages
File Size : 53,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642817847

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Plant Cell and Tissue Culture by J. Reinert,M.M. Yeoman Pdf

The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

Author : John H. Dodds,Lorin W. Roberts
Publisher : International Potato Center
Page : 260 pages
File Size : 50,9 Mb
Release : 1985-10-31
Category : Science
ISBN : 0521315166

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Experiments in Plant Tissue Culture by John H. Dodds,Lorin W. Roberts Pdf

The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Author : Indra K. Vasil,Trevor A. Thorpe
Publisher : Springer Science & Business Media
Page : 593 pages
File Size : 43,8 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401726818

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Plant Cell and Tissue Culture by Indra K. Vasil,Trevor A. Thorpe Pdf

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Author : Michael R. Davey,Paul Anthony
Publisher : John Wiley & Sons
Page : 358 pages
File Size : 41,8 Mb
Release : 2010-05-20
Category : Science
ISBN : 0470686510

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Plant Cell Culture by Michael R. Davey,Paul Anthony Pdf

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Author : Caula A. Beyl,Robert N. Trigiano
Publisher : CRC Press
Page : 482 pages
File Size : 43,5 Mb
Release : 2008-06-09
Category : Science
ISBN : 9781420065091

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Plant Propagation Concepts and Laboratory Exercises by Caula A. Beyl,Robert N. Trigiano Pdf

A complete teaching guide with hands-on laboratories, this book is edited by two of the leading experts in the field. The text develops a working knowledge of the principles of plant propagation, as they apply in temperate and tropical environments. In addition to presenting the essential fundamentals, this carefully conceived w

Author : Angela Mason,Peter Beetham
Publisher : Unknown
Page : 27 pages
File Size : 41,8 Mb
Release : 1986
Category : Plant micropropagation
ISBN : 0730603156

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Plant Tissue Culture Micropropagation Laboratory Manual by Angela Mason,Peter Beetham Pdf

Author : S.S. Bhojwani,M.K. Razdan
Publisher : Elsevier
Page : 766 pages
File Size : 49,9 Mb
Release : 1996-11-08
Category : Science
ISBN : 0080539092

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Plant Tissue Culture: Theory and Practice by S.S. Bhojwani,M.K. Razdan Pdf

Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature. This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.