Expression And Analysis Of Recombinant Ion Channels

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Expression and Analysis of Recombinant Ion Channels

Jeffrey J. Clare,Derek J. Trezise
Expression and Analysis of Recombinant Ion Channels Book PDF

Author : Jeffrey J. Clare,Derek J. Trezise
Publisher : John Wiley & Sons
Page : 302 pages
File Size : 40,9 Mb
Release : 2006-08-21
Category : Science
ISBN : 9783527607938

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Expression and Analysis of Recombinant Ion Channels by Jeffrey J. Clare,Derek J. Trezise Pdf

Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.

Ion Channels

James N. C. Kew,Ceri H. Davies
Ion Channels Book PDF

Author : James N. C. Kew,Ceri H. Davies
Publisher : Unknown
Page : 586 pages
File Size : 55,6 Mb
Release : 2010
Category : Medical
ISBN : 9780199296750

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Ion Channels by James N. C. Kew,Ceri H. Davies Pdf

Ion channels are intimately involved in the everyday physiological functions that enable us to live a full and varied life. When disease strikes, malfunction of ion channels or their dependent is often involved, either as the cause or the effect of the illness. Thus, billions of dollars have been, and still are being, invested in research to understand the physiological and pathophysiological functions of ion channels in an attempt to develop novel therapeutic treatments for a wide range of diseases. This book provides a comprehensive overview of ion channel structure and function. It comprises two major parts. Part one is an introductory overview of the ion channel superfamily and the generic aspects of ion channel function. This part also reviews the methodologies by which ion channel function can be studied from the perspective of performing detailed biophysical characterization through to the deployment of high throughput approaches for identifying novel ion channel ligands. Part two of the book provides an in-depth review of the individual ion channel subfamilies and, as such, is subdivided into four broad sections: Voltage-Gated Ion Channels, Extracellular Ligand-Gated Ion Channels, Intracellular Ligand-Gated Ion Channels, and Polymodal-Gated Ion Channels, with each chapter focused on specific family members. These chapters have been written by world leading experts and provide a detailed overview of the structure, biophysics, localization, pharmacology, physiology, and disease relevance of each particular ion channel subfamily. Reviewing both the basic principles of ion channel function and providing a detailed up-to-date review of the phsyiological and pharmacological aspects of individual ion channel sub-families, this book constitutes both an excellent introduction to the field for non-specialists, as well as a highly valuable reference text for experienced researchers already working in the ion channel area.

Neuroscience Methods

Rosemary Martin
Neuroscience Methods Book PDF

Author : Rosemary Martin
Publisher : CRC Press
Page : 280 pages
File Size : 49,7 Mb
Release : 2019-08-16
Category : Science
ISBN : 9781000725353

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Neuroscience Methods by Rosemary Martin Pdf

Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.

Textbook of Ion Channels

Jie Zheng,Matthew C. Trudeau
Textbook of Ion Channels Book PDF

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 1067 pages
File Size : 48,8 Mb
Release : 2023-07-06
Category : Medical
ISBN : 9781003820239

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Textbook of Ion Channels by Jie Zheng,Matthew C. Trudeau Pdf

The Textbook of Ion Channels is a set of three volumes providing a wide-ranging reference source on ion channels for students, instructors, and researchers. Ion channels are membrane proteins that control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli like light, sound, odor, and taste, and regulate the response to physical stimuli like temperature and pressure. In non-excitable tissues, ion channels are instrumental for the regulation of basic salt balance that is critical for homeostasis. Ion channels are located at the surface membrane of cells, giving them the unique ability to communicate with the environment, as well as the membrane of intracellular organelles, allowing them to regulate internal homeostasis. Ion channels are fundamentally important for human health and diseases, and are important targets for pharmaceuticals in mental illness, heart disease, anesthesia, pain and other clinical applications. The modern methods used in their study are powerful and diverse, ranging from single ion-channel measurement techniques to models of ion channel diseases in animals, and human clinical trials for ion channel drugs. All three volumes give the reader an introduction to fundamental concepts needed to understand the mechanism of ion channels, a guide to the technical aspects of ion channel research, offer a modern guide to the properties of major ion channel families, and include coverage of key examples of regulatory, physiological, and disease roles for ion channels.

Textbook of Ion Channels Volume I

Jie Zheng,Matthew C. Trudeau
Textbook of Ion Channels Volume I Book PDF

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 331 pages
File Size : 54,7 Mb
Release : 2023-06-09
Category : Medical
ISBN : 9781000857757

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Textbook of Ion Channels Volume I by Jie Zheng,Matthew C. Trudeau Pdf

The Textbook of Ion Channels is a set of three volumes providing a wide-ranging reference source on ion channels for students, instructors and researchers. Ion channels are membrane proteins that control the electrical properties of neurons and cardiac cells; mediate the detection and response to sensory stimuli like light, sound, odor, and taste; and regulate the response to physical stimuli like temperature and pressure. In non-excitable tissues, ion channels are instrumental for the regulation of basic salt balance that is critical for homeostasis. Ion channels are located at the surface membrane of cells, giving them the unique ability to communicate with the environment, as well as the membrane of intracellular organelles, allowing them to regulate internal homeostasis. Ion channels are fundamentally important for human health and diseases, and are important targets for pharmaceuticals in mental illness, heart disease, anesthesia, pain and other clinical applications. The modern methods used in their study are powerful and diverse, ranging from single ion-channel measurement techniques to models of ion channel diseases in animals, and human clinical trials for ion channel drugs. Volume I, Part 1 covers fundamental topics such as the basic principles of ion permeation and selectivity, voltage-dependent, ligand-dependent, and mechano-dependent ion channel activation mechanisms, the mechanisms for ion channel desensitization and inactivation, and basic ion channel pharmacology and inhibition. Volume I, Part 2 offers a practical guide of cardinal methods for researching ion channels, including heterologous expression and voltage-clamp and patch-clamp electrophysiology; isolation of native currents using patch clamping; modeling ion channel gating, structures, and its dynamics; crystallography and cryo-electron microscopy; fluorescence and paramagnetic resonance spectroscopy methods; and genetics approaches in model organisms. All three volumes give the reader an introduction to fundamental concepts needed to understand the mechanism of ion channels; a guide to the technical aspects of ion channel research; a modern guide to the properties of major ion channel families; and includes coverage of key examples of regulatory, physiological and disease roles for ion channels.

Receptor and Ion Channel Detection in the Brain

Rafael Luján,Francisco Ciruela
Receptor and Ion Channel Detection in the Brain Book PDF

Author : Rafael Luján,Francisco Ciruela
Publisher : Humana Press
Page : 475 pages
File Size : 51,8 Mb
Release : 2018-03-31
Category : Medical
ISBN : 1493979833

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Receptor and Ion Channel Detection in the Brain by Rafael Luján,Francisco Ciruela Pdf

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

Polypharmacology in Drug Discovery

Jens-Uwe Peters
Polypharmacology in Drug Discovery Book PDF

Author : Jens-Uwe Peters
Publisher : John Wiley & Sons
Page : 542 pages
File Size : 54,8 Mb
Release : 2012-03-13
Category : Medical
ISBN : 9780470590904

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Polypharmacology in Drug Discovery by Jens-Uwe Peters Pdf

An essential outline of the main facets of polypharmacology in drug discovery research Extending drug discovery opportunities beyond the "one drug, one target" philosophy, a polypharmacological approach to the treatment of complex diseases is emerging as a hot topic in both industry and academic research. Polypharmacology in Drug Discovery presents an overview of the various facets of polypharmacology and how it can be applied as an innovative concept for developing medicines for treating bacterial infections, epilepsy, cancer, psychiatric disorders, and more. Filled with a collection of instructive case studies that reinforce the material and illuminate the subject, this practical guide: Covers the two-sided nature of polypharmacology—its contribution to adverse drug reactions and its benefit in certain therapeutic drug classes Addresses the important topic of polypharmacology in drug discovery, a subject that has not been thoroughly covered outside of scattered journal articles Overviews state-of-the-art approaches and developments to help readers understand concepts and issues related to polypharmacology Fosters interdisciplinary drug discovery research by embracing computational, synthetic, in vitro and in vivo pharmacological and clinical aspects of polypharmacology A clear road map for helping readers successfully navigate around the problems involved with promiscuous ligands and targets, Polypharmacology in Drug Discovery provides real examples, in-depth explanations and discussions, and detailed reviews and opinions to spark inspiration for new drug discovery projects.

TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades

Wolfgang B. Liedtke, MD, PH.D.
TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades Book PDF

Author : Wolfgang B. Liedtke, MD, PH.D.
Publisher : CRC Press
Page : 502 pages
File Size : 47,5 Mb
Release : 2006-09-29
Category : Medical
ISBN : 9781420005844

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TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades by Wolfgang B. Liedtke, MD, PH.D. Pdf

Since the first TRP ion channel was discovered in Drosophila melanogaster in 1989, the progress made in this area of signaling research has yielded findings that offer the potential to dramatically impact human health and wellness. Involved in gateway activity for all five of our senses, TRP channels have been shown to respond to a wide range of st

Voltage-Gated Ion Channels as Drug Targets

David J. Triggle,Murali Gopalakrishnan,David Rampe,Wei Zheng
Voltage Gated Ion Channels as Drug Targets Book PDF

Author : David J. Triggle,Murali Gopalakrishnan,David Rampe,Wei Zheng
Publisher : John Wiley & Sons
Page : 492 pages
File Size : 47,5 Mb
Release : 2006-08-21
Category : Science
ISBN : 9783527607747

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Voltage-Gated Ion Channels as Drug Targets by David J. Triggle,Murali Gopalakrishnan,David Rampe,Wei Zheng Pdf

Edited by the most prominent person in the field and top researchers at US pharmaceutical companies, this is a unique resource for drug developers and physiologists seeking a molecular-level understanding of ion channel pharmacology. After an introduction to the topic, the authors evaluate the structure and function of ion channels, as well as related drug interaction. A section on assay technologies is followed by a section each on calcium, sodium and potassium channels. Further chapters cover genetic and acquired channelopathies, before the book closes with a look at safety issues in ion channel drug development. For medicinal and pharmaceutical chemists, biochemists, molecular biologists and those working in the pharmaceutical industry.

Insect Viruses

Byrony C Bonning
Insect Viruses Book PDF

Author : Byrony C Bonning
Publisher : Elsevier
Page : 547 pages
File Size : 54,8 Mb
Release : 2006-09-22
Category : Science
ISBN : 9780080464787

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Insect Viruses by Byrony C Bonning Pdf

Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Ion Channel Drug Discovery

Brian Cox,Martin Gosling
Ion Channel Drug Discovery Book PDF

Author : Brian Cox,Martin Gosling
Publisher : Royal Society of Chemistry
Page : 384 pages
File Size : 55,9 Mb
Release : 2014-09-24
Category : Medical
ISBN : 9781849731867

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Ion Channel Drug Discovery by Brian Cox,Martin Gosling Pdf

A rapidly growing field, this book covers the recent advances in screening technology, ion channel structure and modelling, with up-to-date case histories.

Ion Channels

James D. Stockand,Mark S. Shapiro
Ion Channels Book PDF

Author : James D. Stockand,Mark S. Shapiro
Publisher : Springer Science & Business Media
Page : 208 pages
File Size : 47,6 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450959

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Ion Channels by James D. Stockand,Mark S. Shapiro Pdf

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.

Site and Mechanism of Action of Resin Acids on Voltage-Gated Ion Channels

Malin Silverå Ejneby
Site and Mechanism of Action of Resin Acids on Voltage Gated Ion Channels Book PDF

Author : Malin Silverå Ejneby
Publisher : Linköping University Electronic Press
Page : 58 pages
File Size : 52,7 Mb
Release : 2018-05-15
Category : Cell membranes
ISBN : 9789176853184

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Site and Mechanism of Action of Resin Acids on Voltage-Gated Ion Channels by Malin Silverå Ejneby Pdf

Voltage-gated ion channels are pore-forming membrane proteins that open or close their gates when the voltage across the membrane is changed. They underlie the electrical activity that enables the heart to pump blood and the brain to receive and send signals. Changes in expression, distribution, and functional properties of voltage-gated ion channels can lead to diseases, such as epilepsy, cardiac arrhythmia, and pain-related disorders. Drugs that modulate the function of voltage-gated ion channels control these diseases in some patients, but the existing drugs do not adequately help all patients, and some also have severe side effects. Resin acids are common components of pine resins, with a hydrophobic three-ringed motif and a negatively charged carboxyl group. They open big-conductance Ca2+-activated K+ (BK) channels and voltage-gated potassium (KV) channels. We aimed to characterize the binding site and mechanism of action of resin acids on a KV channel and explore the effect of a resin acid by modifying the position and valence of charge of the carboxyl group. We tested the effect on several voltage-gated ion channels, including two KV channels expressed in Xenopus laevis oocytes and several voltage-gated ion channels expressed in cardiomyocytes. For this endeavour different electrophysiological techniques, ion channels, and cell types were used together with chemical synthesis of about 140 resin-acid derivatives, mathematical models, and computer simulations. We found that resin acids bind between the lipid bilayer and the Shaker KV channel, in the cleft between transmembrane segment S3 and S4, on the extracellular side of the voltage-sensor domain. This is a fundamentally new interaction site for small-molecule compounds that otherwise usually bind to ion channels in pockets surrounded by water. We also showed that the resin acids open the Shaker KV channel via an electrostatic mechanism, exerted on the positively charged voltage sensor S4. The effect of a resin acid increased when the negatively charged carboxyl group (the effector) and the hydrophobic three-ringed motif (anchor in lipid bilayer) were separated by three atoms: longer stalks decreased the effect. The length rule, in combination with modifications of the anchor, was used to design new resin-acid derivatives that open the human M-type (Kv7.2/7.3) channel. A naturally occurring resin acid also reduced the excitability of cardiomyocytes by affecting the voltage-dependence of several voltage-gated ion channels. The major finding was that the resin acid inactivated sodium and calcium channels, while it activated KV channels at more negative membrane voltages. Computer simulations confirmed that the combined effect on different ion channels reduced the excitability of a cardiomyocyte. Finally, the resin acid reversed induced arrhythmic firing of the cardiomyocytes. In conclusion, resin acids are potential drug candidates for diseases such as epilepsy and cardiac arrhythmia: knowing the binding site and mechanism of action can help to fine tune the resin acid to increase the effect, as well as the selectivity.

Pharmacology of Potassium Channels

Nikita Gamper,KeWei Wang
Pharmacology of Potassium Channels Book PDF

Author : Nikita Gamper,KeWei Wang
Publisher : Springer Nature
Page : 546 pages
File Size : 55,8 Mb
Release : 2021-09-27
Category : Medical
ISBN : 9783030840525

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Pharmacology of Potassium Channels by Nikita Gamper,KeWei Wang Pdf

The aim of the present book is to comprehensively review current advances in understanding of genetics, structural biology, pharmacology of potassium channels and their roles in disease as well as to identify current gaps in knowledge. The ultimate goal is to provide a scientific foundation for better understanding of modulatory mechanisms and pharmacology of potassium channels and to use this understanding to drive future drug discovery. This book will be a must-have for academic and industrial scientists interested in physiology, pharmacology, pathology and structure-functional relationships of ion channels. The book will also be helpful for lecturers and students in the college and university classrooms, as well as for anyone interested in the state-of-the art in modern cell biology, physiology and pharmacology.

Patch Clamp Electrophysiology

Mark Dallas,Damian Bell
Patch Clamp Electrophysiology Book PDF

Author : Mark Dallas,Damian Bell
Publisher : Humana
Page : 333 pages
File Size : 48,6 Mb
Release : 2021-11-13
Category : Medical
ISBN : 1071608207

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Patch Clamp Electrophysiology by Mark Dallas,Damian Bell Pdf

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.