Fluorescence Microscopy In Life Sciences

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Fluorescence Microscopy in Life Sciences

Juan Carlos Stockert,Alfonso Blázquez-Castro
Fluorescence Microscopy in Life Sciences Book PDF

Author : Juan Carlos Stockert,Alfonso Blázquez-Castro
Publisher : Unknown
Page : 128 pages
File Size : 47,7 Mb
Release : 2017-12-15
Category : Electronic
ISBN : 1681085194

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Fluorescence Microscopy in Life Sciences by Juan Carlos Stockert,Alfonso Blázquez-Castro Pdf

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained. Key learning points covered in the book include: Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology Fluorescent chemical compounds Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques Fluorescence Microscopy In Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.

Fluorescence Microscopy in Life Sciences

Juan Carlos Stockert ,Alfonso Blazquez-Castro
Fluorescence Microscopy in Life Sciences Book PDF

Author : Juan Carlos Stockert ,Alfonso Blazquez-Castro
Publisher : Bentham Science Publishers
Page : 751 pages
File Size : 54,7 Mb
Release : 2017-12-15
Category : Science
ISBN : 9781681085180

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Fluorescence Microscopy in Life Sciences by Juan Carlos Stockert ,Alfonso Blazquez-Castro Pdf

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

Fluorescence Microscopy

Ulrich Kubitscheck
Fluorescence Microscopy Book PDF

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 40,5 Mb
Release : 2017-03-27
Category : Medical
ISBN : 9783527687725

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy

Anda Cornea,P. Michael Conn
Fluorescence Microscopy Book PDF

Author : Anda Cornea,P. Michael Conn
Publisher : Elsevier
Page : 261 pages
File Size : 51,8 Mb
Release : 2014-02-24
Category : Science
ISBN : 9780124167131

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Fluorescence Microscopy by Anda Cornea,P. Michael Conn Pdf

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Fluorescence Applications in Biotechnology and Life Sciences

Ewa M. Goldys
Fluorescence Applications in Biotechnology and Life Sciences Book PDF

Author : Ewa M. Goldys
Publisher : John Wiley & Sons
Page : 398 pages
File Size : 48,9 Mb
Release : 2009-08-24
Category : Science
ISBN : 9780470083703

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Fluorescence Applications in Biotechnology and Life Sciences by Ewa M. Goldys Pdf

A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.

Fluorescence Microscopy of Living Cells in Culture, Part B

Anonim
Fluorescence Microscopy of Living Cells in Culture Part B Book PDF

Author : Anonim
Publisher : Academic Press
Page : 502 pages
File Size : 43,5 Mb
Release : 1989-04-01
Category : Science
ISBN : 0080859283

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Fluorescence Microscopy of Living Cells in Culture, Part B by Anonim Pdf

Fluorescence Microscopy of Living Cells in Culture, Part B

Fundamentals of Fluorescence Microscopy

Partha Pratim Mondal,Alberto Diaspro
Fundamentals of Fluorescence Microscopy Book PDF

Author : Partha Pratim Mondal,Alberto Diaspro
Publisher : Springer Science & Business Media
Page : 218 pages
File Size : 50,8 Mb
Release : 2013-12-12
Category : Science
ISBN : 9789400775459

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Fundamentals of Fluorescence Microscopy by Partha Pratim Mondal,Alberto Diaspro Pdf

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Fluorescence Microscopy and Fluorescent Probes

J. Slavík
Fluorescence Microscopy and Fluorescent Probes Book PDF

Author : J. Slavík
Publisher : Springer Science & Business Media
Page : 338 pages
File Size : 53,9 Mb
Release : 1996-10-31
Category : Science
ISBN : 0306453924

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Fluorescence Microscopy and Fluorescent Probes by J. Slavík Pdf

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Karsten König
Multiphoton Microscopy and Fluorescence Lifetime Imaging Book PDF

Author : Karsten König
Publisher : Walter de Gruyter GmbH & Co KG
Page : 450 pages
File Size : 45,7 Mb
Release : 2018-01-22
Category : Science
ISBN : 9783110429985

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Multiphoton Microscopy and Fluorescence Lifetime Imaging by Karsten König Pdf

This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology.

Fluorescence Microscopy

Brian Herman
Fluorescence Microscopy Book PDF

Author : Brian Herman
Publisher : Garland Science
Page : 0 pages
File Size : 47,7 Mb
Release : 1998
Category : Fluorescence microscopy
ISBN : 1872748848

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Fluorescence Microscopy by Brian Herman Pdf

Fluoresence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope and other required equipment is given, too. Applications to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical detail. Troubleshooting guidance is provided, along with many practical hints, to guide the user through commonly encountered problems.

Fluorescent Microscopy

Bryan Heit
Fluorescent Microscopy Book PDF

Author : Bryan Heit
Publisher : Humana
Page : 0 pages
File Size : 45,7 Mb
Release : 2023-03-13
Category : Science
ISBN : 1071620533

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Fluorescent Microscopy by Bryan Heit Pdf

This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field.

Light Microscopy in Biology

Alan J. Lacey
Light Microscopy in Biology Book PDF

Author : Alan J. Lacey
Publisher : OUP Oxford
Page : 475 pages
File Size : 50,7 Mb
Release : 1999-06-03
Category : Science
ISBN : 9780191565724

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Light Microscopy in Biology by Alan J. Lacey Pdf

Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.

Optical Fluorescence Microscopy

Alberto Diaspro
Optical Fluorescence Microscopy Book PDF

Author : Alberto Diaspro
Publisher : Springer Science & Business Media
Page : 252 pages
File Size : 46,7 Mb
Release : 2010-11-25
Category : Science
ISBN : 9783642151750

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Optical Fluorescence Microscopy by Alberto Diaspro Pdf

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Fluorescence Microscopy

F. W. D. Rost
Fluorescence Microscopy Book PDF

Author : F. W. D. Rost
Publisher : Cambridge University Press
Page : 486 pages
File Size : 55,7 Mb
Release : 1992
Category : Science
ISBN : 0521410886

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Fluorescence Microscopy by F. W. D. Rost Pdf

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Microscopic Image Analysis for Life Science Applications

Jens Rittscher,Raghu Machiraju,Stephen T. C. Wong
Microscopic Image Analysis for Life Science Applications Book PDF

Author : Jens Rittscher,Raghu Machiraju,Stephen T. C. Wong
Publisher : Artech House
Page : 533 pages
File Size : 46,7 Mb
Release : 2008
Category : Medical
ISBN : 9781596932371

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Microscopic Image Analysis for Life Science Applications by Jens Rittscher,Raghu Machiraju,Stephen T. C. Wong Pdf

Here's a first-of-its-kind book that bridges the gap between biomedical imaging and the bioscience community. This unique resource gives you a detailed understanding of imaging platforms, fluorescence imaging, and fundamental image processing algorithms. Further, it guides you through application of advanced image analysis methods and techniques to specific biological problems. The book presents applications that span a wide range of scales, from the detection of signaling events in sub-cellular structures, to the automated analysis of tissue structures. Other critical areas discussed include the dynamics of cell populations and in vivo microscopy. This cutting-edge volume is supported with over 160 illustrations that support key topics throughout the book. CD-ROM Included! Contains full-color images and videos that further illustrate topics discussed in the book.