Fluorescence Microscopy

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Fluorescence Microscopy

Ulrich Kubitscheck
Fluorescence Microscopy Book PDF

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 54,9 Mb
Release : 2017-03-27
Category : Medical
ISBN : 9783527687725

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy

Anda Cornea,P. Michael Conn
Fluorescence Microscopy Book PDF

Author : Anda Cornea,P. Michael Conn
Publisher : Elsevier
Page : 260 pages
File Size : 41,9 Mb
Release : 2014-02-24
Category : Science
ISBN : 9780124167131

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Fluorescence Microscopy by Anda Cornea,P. Michael Conn Pdf

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Introduction to Confocal Fluorescence Microscopy

Michiel Muller
Introduction to Confocal Fluorescence Microscopy Book PDF

Author : Michiel Muller
Publisher : SPIE Press
Page : 142 pages
File Size : 53,5 Mb
Release : 2006
Category : Science
ISBN : 0819460435

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Introduction to Confocal Fluorescence Microscopy by Michiel Muller Pdf

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Fundamentals of Fluorescence Microscopy

Partha Pratim Mondal,Alberto Diaspro
Fundamentals of Fluorescence Microscopy Book PDF

Author : Partha Pratim Mondal,Alberto Diaspro
Publisher : Springer Science & Business Media
Page : 218 pages
File Size : 51,8 Mb
Release : 2013-12-12
Category : Science
ISBN : 9789400775459

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Fundamentals of Fluorescence Microscopy by Partha Pratim Mondal,Alberto Diaspro Pdf

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Fluorescence Microscopy in Life Sciences

Juan Carlos Stockert ,Alfonso Blazquez-Castro
Fluorescence Microscopy in Life Sciences Book PDF

Author : Juan Carlos Stockert ,Alfonso Blazquez-Castro
Publisher : Bentham Science Publishers
Page : 751 pages
File Size : 41,6 Mb
Release : 2017-12-15
Category : Science
ISBN : 9781681085180

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Fluorescence Microscopy in Life Sciences by Juan Carlos Stockert ,Alfonso Blazquez-Castro Pdf

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

Flourescence Microscopy

Brian Herman
Flourescence Microscopy Book PDF

Author : Brian Herman
Publisher : Springer
Page : 188 pages
File Size : 46,7 Mb
Release : 1998
Category : Medical
ISBN : UOM:49015002477850

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Flourescence Microscopy by Brian Herman Pdf

The fluorescence microscope is an important biological laboratory tool, used to detect dells or molecules that emit fluorescence. This book deals with the fundamental principles and techniques, covering topics such as immunofluorescence, in-situ hybridization, and fluorescence photomicrography. In addition, a complete troubleshooting guide with practical hints is provided. 60 illus.

Fluorescence Microscopy

F. W. D. Rost
Fluorescence Microscopy Book PDF

Author : F. W. D. Rost
Publisher : Cambridge University Press
Page : 486 pages
File Size : 42,9 Mb
Release : 1992
Category : Science
ISBN : 0521410886

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Fluorescence Microscopy by F. W. D. Rost Pdf

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Introduction to Fluorescence Microscopy

J. S. Ploem,H. J. Tanke
Introduction to Fluorescence Microscopy Book PDF

Author : J. S. Ploem,H. J. Tanke
Publisher : Garland Science
Page : 72 pages
File Size : 44,9 Mb
Release : 1987
Category : Language Arts & Disciplines
ISBN : UOM:39015012610625

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Introduction to Fluorescence Microscopy by J. S. Ploem,H. J. Tanke Pdf

Covers three main areas.The phenomenon of fluorescence, the main applications, and the most common problems.

Quantitative Fluorescence Microscopy

F. W. D. Rost
Quantitative Fluorescence Microscopy Book PDF

Author : F. W. D. Rost
Publisher : Cambridge University Press
Page : 264 pages
File Size : 48,6 Mb
Release : 1991-07-26
Category : Science
ISBN : 0521394228

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Quantitative Fluorescence Microscopy by F. W. D. Rost Pdf

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.

Optical Fluorescence Microscopy

Alberto Diaspro
Optical Fluorescence Microscopy Book PDF

Author : Alberto Diaspro
Publisher : Springer Science & Business Media
Page : 252 pages
File Size : 42,9 Mb
Release : 2010-11-25
Category : Science
ISBN : 9783642151750

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Optical Fluorescence Microscopy by Alberto Diaspro Pdf

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Fluorescence Microscopy

Ulrich Kubitscheck
Fluorescence Microscopy Book PDF

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 504 pages
File Size : 46,9 Mb
Release : 2017-06-19
Category : Medical
ISBN : 9783527338375

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Karsten König
Multiphoton Microscopy and Fluorescence Lifetime Imaging Book PDF

Author : Karsten König
Publisher : Walter de Gruyter GmbH & Co KG
Page : 450 pages
File Size : 51,9 Mb
Release : 2018-01-22
Category : Science
ISBN : 9783110430073

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Multiphoton Microscopy and Fluorescence Lifetime Imaging by Karsten König Pdf

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Fluorescence Microscopy

B. Herman
Fluorescence Microscopy Book PDF

Author : B. Herman
Publisher : Taylor & Francis
Page : 185 pages
File Size : 48,7 Mb
Release : 2020-08-26
Category : Science
ISBN : 9781000102505

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Fluorescence Microscopy by B. Herman Pdf

This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.

Fluorescence Microscopy of Living Cells in Culture, Part A

Anonim
Fluorescence Microscopy of Living Cells in Culture Part A Book PDF

Author : Anonim
Publisher : Academic Press
Page : 332 pages
File Size : 42,8 Mb
Release : 1989-02-01
Category : Science
ISBN : 0080859275

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Fluorescence Microscopy of Living Cells in Culture, Part A by Anonim Pdf

Fluorescence Microscopy of Living Cells in Culture, Part A

Protein Localization by Fluorescence Microscopy

Viki Allan
Protein Localization by Fluorescence Microscopy Book PDF

Author : Viki Allan
Publisher : OUP Oxford
Page : 258 pages
File Size : 48,5 Mb
Release : 1999-11-18
Category : Science
ISBN : 9780191565915

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Protein Localization by Fluorescence Microscopy by Viki Allan Pdf

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.