Forensic Dna Profiling Protocols

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Forensic DNA Profiling Protocols

Patrick J. Lincoln,Jim Thomson
Forensic DNA Profiling Protocols Book PDF

Author : Patrick J. Lincoln,Jim Thomson
Publisher : Springer Science & Business Media
Page : 617 pages
File Size : 43,7 Mb
Release : 1998-01-22
Category : Medical
ISBN : 9780896034433

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Forensic DNA Profiling Protocols by Patrick J. Lincoln,Jim Thomson Pdf

This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.

Forensic DNA Typing Protocols

Angel Carracedo
Forensic DNA Typing Protocols Book PDF

Author : Angel Carracedo
Publisher : Springer Science & Business Media
Page : 282 pages
File Size : 42,7 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592598670

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Forensic DNA Typing Protocols by Angel Carracedo Pdf

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

DNA Technology in Forensic Science

National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Technology in Forensic Science
DNA Technology in Forensic Science Book PDF

Author : National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Technology in Forensic Science
Publisher : National Academies Press
Page : 199 pages
File Size : 52,7 Mb
Release : 1992-02-01
Category : Science
ISBN : 9780309045872

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DNA Technology in Forensic Science by National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Technology in Forensic Science Pdf

Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.

Forensic DNA Analysis

Catherine Cupples Connon
Forensic DNA Analysis Book PDF

Author : Catherine Cupples Connon
Publisher : Springer Nature
Page : 423 pages
File Size : 53,5 Mb
Release : 2023-08-14
Category : Science
ISBN : 9781071632956

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Forensic DNA Analysis by Catherine Cupples Connon Pdf

This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.

Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing

Hirak Ranjan Dash,Pankaj Shrivastava,Surajit Das
Principles and Practices of DNA Analysis A Laboratory Manual for Forensic DNA Typing Book PDF

Author : Hirak Ranjan Dash,Pankaj Shrivastava,Surajit Das
Publisher : Humana
Page : 0 pages
File Size : 55,6 Mb
Release : 2020-02-07
Category : Medical
ISBN : 107160273X

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Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing by Hirak Ranjan Dash,Pankaj Shrivastava,Surajit Das Pdf

The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.

Forensic DNA Profiling Protocols. Methods in Molecular Biology

Patrick J. Lincoln,Jim Thomson
Forensic DNA Profiling Protocols Methods in Molecular Biology Book PDF

Author : Patrick J. Lincoln,Jim Thomson
Publisher : Unknown
Page : 128 pages
File Size : 55,6 Mb
Release : 1998
Category : Electronic
ISBN : OCLC:748540346

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Forensic DNA Profiling Protocols. Methods in Molecular Biology by Patrick J. Lincoln,Jim Thomson Pdf

Leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR- based test systems the now widely used STR typing systems (complete with details of both manual and automated detection systems) repeat unit mapping (MVR) direct phase minisequencing capillary electrophoresis and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood semen skeletal remains and saliva) species testing sex determination and mitochondrial DNA testing. SMP Version: This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types analysis of DNA as single and multi-locus VNTR probes PCR amplification of STR and other loci and mitochondrial sequencing.; The expert scientists writing here-many from laboratories around the world-also discuss how to interpret the results in cases of unknown identity and disputed parentage.

The Evaluation of Forensic DNA Evidence

National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Forensic Science: An Update
The Evaluation of Forensic DNA Evidence Book PDF

Author : National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Forensic Science: An Update
Publisher : National Academies Press
Page : 271 pages
File Size : 40,9 Mb
Release : 1997-01-12
Category : Science
ISBN : 9780309121941

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The Evaluation of Forensic DNA Evidence by National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Forensic Science: An Update Pdf

In 1992 the National Research Council issued DNA Technology in Forensic Science, a book that documented the state of the art in this emerging field. Recently, this volume was brought to worldwide attention in the murder trial of celebrity O. J. Simpson. The Evaluation of Forensic DNA Evidence reports on developments in population genetics and statistics since the original volume was published. The committee comments on statements in the original book that proved controversial or that have been misapplied in the courts. This volume offers recommendations for handling DNA samples, performing calculations, and other aspects of using DNA as a forensic toolâ€"modifying some recommendations presented in the 1992 volume. The update addresses two major areas: Determination of DNA profiles. The committee considers how laboratory errors (particularly false matches) can arise, how errors might be reduced, and how to take into account the fact that the error rate can never be reduced to zero. Interpretation of a finding that the DNA profile of a suspect or victim matches the evidence DNA. The committee addresses controversies in population genetics, exploring the problems that arise from the mixture of groups and subgroups in the American population and how this substructure can be accounted for in calculating frequencies. This volume examines statistical issues in interpreting frequencies as probabilities, including adjustments when a suspect is found through a database search. The committee includes a detailed discussion of what its recommendations would mean in the courtroom, with numerous case citations. By resolving several remaining issues in the evaluation of this increasingly important area of forensic evidence, this technical update will be important to forensic scientists and population geneticistsâ€"and helpful to attorneys, judges, and others who need to understand DNA and the law. Anyone working in laboratories and in the courts or anyone studying this issue should own this book.

A Guide to Forensic DNA Profiling

Scott Bader
A Guide to Forensic DNA Profiling Book PDF

Author : Scott Bader
Publisher : John Wiley & Sons
Page : 951 pages
File Size : 47,6 Mb
Release : 2016-03-08
Category : Law
ISBN : 9781118751510

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A Guide to Forensic DNA Profiling by Scott Bader Pdf

The increasingly arcane world of DNA profiling demands that those needing to understand at least some of it must find a source of reliable and understandable information. Combining material from the successful Wiley Encyclopedia of Forensic Science with newly commissioned and updated material, the Editors have used their own extensive experience in criminal casework across the world to compile an informative guide that will provide knowledge and thought-provoking articles of interest to anyone involved or interested in the use of DNA in the forensic context. Following extensive introductory chapters covering forensic DNA profiling and forensic genetics, this comprehensive volume presents a substantial breadth of material covering: Fundamental material – including sources of DNA, validation, and accreditation Analysis and interpretation – including, extraction, quantification, amplification and interpretation of electropherograms (epgs) Evaluation – including mixtures, low template, and transfer Applications – databases, paternity and kinship, mitochondrial-DNA, wildlife DNA, single-nucleotide polymorphism, phenotyping and familial searching Court - report writing, discovery, cross examination, and current controversies With contributions from leading experts across the whole gamut of forensic science, this volume is intended to be authoritative but not authoritarian, informative but comprehensible, and comprehensive but concise. It will prove to be a valuable addition, and useful resource, for scientists, lawyers, teachers, criminologists, and judges.

DNA Electrophoresis Protocols for Forensic Genetics

Antonio Alonso
DNA Electrophoresis Protocols for Forensic Genetics Book PDF

Author : Antonio Alonso
Publisher : Humana Press
Page : 394 pages
File Size : 44,7 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493962191

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DNA Electrophoresis Protocols for Forensic Genetics by Antonio Alonso Pdf

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Forensic DNA Profiling

Jo-Anne Bright,Michael Coble
Forensic DNA Profiling Book PDF

Author : Jo-Anne Bright,Michael Coble
Publisher : CRC Press
Page : 132 pages
File Size : 51,8 Mb
Release : 2019-12-09
Category : Law
ISBN : 9780429671425

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Forensic DNA Profiling by Jo-Anne Bright,Michael Coble Pdf

DNA testing and its forensic analysis are recognized as the “gold standard” in forensic identification science methods. However, there is a great need for a hands-on step-by-step guide to teach the forensic DNA community how to interpret DNA mixtures, how to assign a likelihood ratio, and how to use the subsequent likelihood ratio when reporting interpretation conclusions. Forensic DNA Profiling: A Practical Guide to Assigning Likelihood Ratios will provide a roadmap for labs all over the world and the next generation of analysts who need this foundational understanding. The techniques used in forensic DNA analysis are based upon the accepted principles of molecular biology. The interpretation of a good-quality DNA profile generated from a crime scene stain from a single-source donor provides an unambiguous result when using the most modern forensic DNA methods. Unfortunately, many crime scene profiles are not single source. They are described as mixed since they contain DNA from two or more individuals. Interpretation of DNA mixtures represents one of the greatest challenges to the forensic DNA analyst. As such, the book introduces terms used to describe DNA profiles and profile interpretation. Chapters explain DNA extraction methods, the polymerase chain reaction (PCR), capillary electrophoresis (CE), likelihood ratios (LRs) and their interpretation, and population genetic models—including Mendelian inheritance and Hardy-Weinberg equilibrium. It is important that analysts understand how LRs are generated in a probabilistic framework, ideally with an appreciation of both semicontinuous and fully continuous probabilistic approaches. KEY FEATURES: • The first book to focus entirely on DNA mixtures and the complexities involved with interpreting the results • Takes a hands-on approach offering theory with worked examples and exercises to be easily understood and implementable by laboratory personnel • New methods, heretofore unpublished previously, provide a means to innovate deconvoluting a mixed DNA profile, assign an LR, and appropriately report the weight of evidence • Includes a chapter on assigning LRs for close relatives (i.e., “It’s not me, it was my brother”), and discusses strategies for the validation of probabilistic genotyping software Forensic DNA Profiling fills the void for labs unfamiliar with LRs, and moving to probabilistic solutions, and for labs already familiar with LRs, but wishing to understand how they are calculated in more detail. The book will be a welcome read for lab professionals and technicians, students, and legal professionals seeking to understand and apply the techniques covered.

Handbook of DNA Profiling

Hirak Ranjan Dash,Pankaj Shrivastava,J. A. Lorente
Handbook of DNA Profiling Book PDF

Author : Hirak Ranjan Dash,Pankaj Shrivastava,J. A. Lorente
Publisher : Springer
Page : 1206 pages
File Size : 41,9 Mb
Release : 2022-06-29
Category : Science
ISBN : 9811643172

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Handbook of DNA Profiling by Hirak Ranjan Dash,Pankaj Shrivastava,J. A. Lorente Pdf

This reference book comprehensively reviews the significance of DNA technology in forensic science. After presenting the theory, basic principles, tools and techniques that are used in forensic DNA typing, it summarizes various techniques, including autosomal STR, Y-STR, X-STR, mitochondrial DNA and NGS, used in solving both criminal as and civil cases, such as paternity disputes, identification of mutilated remains, and culprit identification in sexual assault and murder cases. It also provides an overview of DNA-based genetic diagnostics for various diseases, and discusses the role of DNA typing in drug reactions, as well as the application of non-human DNA profiling of animals and plants in forensic science investigations. Lastly, the book examines the role of internal quality control in maintaining the high quality of DNA profiling.

Advanced Topics in Forensic DNA Typing: Interpretation

John M. Butler
Advanced Topics in Forensic DNA Typing Interpretation Book PDF

Author : John M. Butler
Publisher : Academic Press
Page : 609 pages
File Size : 49,5 Mb
Release : 2014-07-28
Category : Social Science
ISBN : 9780124058545

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Advanced Topics in Forensic DNA Typing: Interpretation by John M. Butler Pdf

Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler’s internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence Worked mixture examples illustrate the impact of different statistical approaches for reporting results Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011

Introduction to Forensic DNA Analysis

Keith Inman,Norah Rudin
Introduction to Forensic DNA Analysis Book PDF

Author : Keith Inman,Norah Rudin
Publisher : CRC Press
Page : 284 pages
File Size : 54,9 Mb
Release : 1997-02-07
Category : Law
ISBN : UOM:39015040619440

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Introduction to Forensic DNA Analysis by Keith Inman,Norah Rudin Pdf

This book emphasizes the advantages and limitations of using DNA techniques for the presentation of evidence in the courtroom and in the general development of various types of criminal cases. The authors present the material in an understandable manner for use by professionals in the legal system, as well as those in the fields of forensics and law enforcement. Coverage includes: Key terminology used in the field The scientific basis of DNA typing Statistical interpretations of DNA typing A summary of court decisions and admissibility standards

Forensic DNA Analysis

Jaiprakash G. Shewale,Ray H. Liu
Forensic DNA Analysis Book PDF

Author : Jaiprakash G. Shewale,Ray H. Liu
Publisher : CRC Press
Page : 449 pages
File Size : 52,7 Mb
Release : 2013-08-19
Category : Law
ISBN : 9781466571266

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Forensic DNA Analysis by Jaiprakash G. Shewale,Ray H. Liu Pdf

The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing, forensic casework, family lineage studies, identification of human remains, and DNA databasing. Forensic DNA Analysis: Current Practices and Emerging Technologies explores the fundamental principles and the application of technologies for each aspect of forensic DNA analysis. The book begins by discussing the value of DNA evidence and how to properly recognize, document, collect, and store it. The remaining chapters examine: The most widely adopted methods and the best practices for DNA isolation from forensic biological samples and human remains Studies carried out on the use of both messenger RNA and small (micro) RNA profiling Real-time polymerase chain reaction (PCR) methods for quantification and assessment of human DNA prior to genotyping Capillary electrophoresis (CE) as a tool for forensic DNA analysis Next-generation short tandem repeat (STR) genotyping kits for forensic applications, the biological nature of STR loci, and Y-chromosome STRs (Y-STRs) Mitochondrial DNA (mtDNA) sequence analysis Single nucleotide polymorphisms (SNPs) and insertion/deletion polymorphisms (indels) in typing highly degraded DNA Deep-sequencing technologies The current state of integrated systems in forensic DNA analysis The book concludes by discussing various aspects of sample-processing training and the entities that provide such training programs. This volume is an essential resource for students, researchers, teaching faculties, and other professionals interested in human identification/forensic DNA analysis.

Forensic DNA Typing: Principles, Applications and Advancements

Pankaj Shrivastava,Hirak Ranjan Dash,Jose A. Lorente,Jahangir Imam
Forensic DNA Typing Principles Applications and Advancements Book PDF

Author : Pankaj Shrivastava,Hirak Ranjan Dash,Jose A. Lorente,Jahangir Imam
Publisher : Springer Nature
Page : 673 pages
File Size : 52,6 Mb
Release : 2020-12-11
Category : Science
ISBN : 9789811566554

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Forensic DNA Typing: Principles, Applications and Advancements by Pankaj Shrivastava,Hirak Ranjan Dash,Jose A. Lorente,Jahangir Imam Pdf

The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.